1.A New Sesterterpene from the Korean Sarcotragus sp. Sponge.
Jung Kyun WOO ; Ju Eun JEON ; Bora KIM ; Chung J SIM ; Dong Chan OH ; Ki Bong OH ; Jongheon SHIN
Natural Product Sciences 2015;21(4):237-239
Sarcotragin C (1), a new sesterterpene metabolite was isolated from a Sarcotragus sp. sponge collected from Chuja Island, Korea. On the basis of the combined spectroscopic analyses, the structure of this compound was determined to be a linear norsesterterpene containing a leucine-derived gamma-lactam moiety. This compound exhibited moderate cytotoxicity against K562 and A549 cell-lines.
Korea
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Porifera*
2.Study on isolation of two components with biological activities from the Species of sponge Dysidea cinerea collected from Ha Long maritime area
Pharmaceutical Journal 2005;0(1):10-12
Dys-1 and Dys-2 compounds were isolated and determined structure from extracts of n-hexane and dichloromethane of sponge Dysidea cinerea by normal and reverse phases of silica gel column chromatography. Dys-1 is a white crystallized agent. Through data and reference materials, the agent is determined as Isofucosterol (24-Stigmasta-5, 24(24')-dien-3β-ol). Dys-2 compound is a crystallized agent with crystal of white needle form. The compound is determined as 5, 8- epidioxycholest-6--en-3-ol. It is cyto-toxic against three tumor cell lines in human such as epithelium cancer, liver cancer, and uterus membrane cancer
Porifera
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Chromatography
3.Modification of surface pretreatment of white spot lesions to improve the safety and efficacy of resin infiltration.
Hyun Kyung YIM ; Ji Hyun MIN ; Ho Keun KWON ; Baek Il KIM
The Korean Journal of Orthodontics 2014;44(4):195-202
OBJECTIVE: A low-viscosity resin (infiltrant) was used to inhibit the progression of white spot lesions (WSLs) and resolve associated esthetic issues. An alternative pretreatment was explored to increase the pore volume of the surface layer of the WSLs. Also, the penetration effects of the infiltrant were evaluated for various pretreatments. METHODS: Sixty two artificial lesions were fabricated on bovine teeth. As a positive control, 15% HCl gel was applied for 120 seconds. Further, 37% H3PO4 gel was applied for 30 seconds using three methods. The samples were divided as follows: H3PO4 only group, H3PO4 sponge group, and H3PO4 brush group. The acid was gently rubbed with the applicators (i.e., a sponge or brush) throughout the application time. To compare the effects of resin infiltration, twenty paired halves of specimens were treated with an infiltrant (ICON(R)). RESULTS: Thicknesses of the removed surface layers and infiltrated areas were evaluated by confocal laser scanning microscope. The positive control and the 37% H3PO4 brush group failed to show significant differences in the removed thickness (p > 0.05); however, the mean percentage of the infiltrated area was higher in the 37% H3PO4 brush group (84.13 +/- 7.58%) than the positive control (63.51 +/- 7.62%, p < 0.001). Scanning electron microscope observations indicate higher pore volumes for the 37% H3PO4 brush group than for the positive control. CONCLUSIONS: Application of 37% H3PO4 with a brush for 30 seconds increased the pore volume of WSL surface layers and the percentage of infiltrated areas in comparison to the use of 15% HCl for 120 seconds.
Dental Caries*
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Porifera
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Tooth
4.Improved stress analyses of dental systems implant by homogenization technique.
Chul Su KOH ; Man Sup LEE ; Kui Won CHOI
The Journal of the Korean Academy of Periodontology 1997;27(1):263-290
Homogenization technique is employed to investigate the series of stress analyses of mandible for three different types of dental implants. This technique helps to make proper material model of bone and analyze such a non homogeneous structure at the level of individual microstructural unit. The stress analyses with homogenization technique show much higher stress level in the sponge bone, compared to those of conventional FEM. It also manifested that even a minor lateral force results in crucial stresses in the dental implant system and that the macroscale model should take the shape and size after real mandible to produce reasonable solution in the analyses of dental implant systems. The shapes of dental implants simulated in this study are rectangularcross-sectioned type, hemi-sphere rooted type, and wedge type implant. The stress states of mandible with hemisphere rooted type implant and wedge type implant show similar levels, while those with sectioned rectangular implant results in higher stresses. It is suggested that the distance between the implant tip and cortical bone be kept far enough to prevent stress concentrations in the mandible.
Dental Implants
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Mandible
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Porifera
5.Two New Scalaranes from a Korean Marine Sponge Spongia sp..
Inho YANG ; Sang Jip NAM ; Heonjoong KANG
Natural Product Sciences 2015;21(4):289-292
Intensive chemical investigation of Korean marine sponge Spongia sp. has led to the isolation of two new scalaranes. The planar structures of the new compounds 1 and 2 were determined through 1D and 2D NMR spectral data analysis, while the relative stereochemistry of the compounds was determined based on the analysis of 1H-1H coupling constants and NOESY spectroscopic data. Compounds 1 and 2 did not display any significant biological activities on farnesoid X-activated receptor (FXR) in co-transfection assay.
Porifera*
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Statistics as Topic
6.The effects of tetracycline-HCl on SLA implant surface structure.
Mi Ran SEO ; Joon Bong PARK ; Young Hyuk KWON ; Yeek HERR ; Jong Hyuk CHUNG
The Journal of the Korean Academy of Periodontology 2007;37(2):251-263
The present study was performed to evaluate the effects of Tetracycline-HCl on the microstructure change of SLA implant surface according to application time. In the Tetracycline-HCl group, 6 implants were rubbed with sponges soaked 50mg/ml Tetracycline-HCl solution for 0.5min., 1min., 1.5min., 2min., 2.5min. and 3min. In the saline group, another 6 implants conditioned with sponges soaked saline using same methods. One implant wasn't conditioned anything. Then, the changes of surface roughness values were evaluated by optical interferometer & specimens were processed for scanning electron microscopic observation. The results of this study were as follows: 1. In both Tetracycline-HCl group & saline group, there are no significant differences between surface roughness values before & after surface detoxification. And in scanning electron microscopic observation, there are slightl9y changes of implant surface structures but this changes were not significant by comparison with no treatment implant surface. 2. In the changes of surface roughness values & the scanning electron microscopic observation, there were no significant differences between saline & Tetracycline-HCl groups. In conclusion, the detoxification with 50mg/ml Tetracycline-HCl within 3 minutes can be applied for treatment of peri-implantitis in SLA surface implants, without surface microstructure changes.
Peri-Implantitis
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Porifera
7.Aspiration of a sponge during conscious sedation.
Ji Young LEE ; Jin Young CHON ; Hyun Jung KOH ; Yu Mi JU ; Mi Ran PARK
Korean Journal of Anesthesiology 2013;65(6 Suppl):S14-S15
No abstract available.
Conscious Sedation*
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Porifera*
8.Culture of Chondrocytes on Scaffolds with Different Pore Network of PLGA and PLLA.
Jong Won RHIE ; Tae Joo AHN ; Jae Gu PARK ; Joo Young SOHN ; Hae Suk CHO ; Poong LIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2003;30(2):237-244
This study was performed to investigate the in vitro proliferation and migration of rabbit auricular chondrocytes into the various sized pore of PLLA and PLGA scaffolds. The chondrocytes were harvested, expanded, and seeded onto PLGA(50 : 50, 75 : 25, 85 : 15) and PLLA scaffold having either small(50 - 100 micrometer) or large(300 - 350 micrometer) pores. On the 4th and 8th week after culture, histologic observation and quantitative DNA assay were done. We noted that the largest amount of DNA was found in the 85 : 15 PLGA sponges than others, and in the 4th and 8th week, some amount of DNA was detected in the lower portion of 85 : 15 PLGA sponge only, and DNA amounts were increased during the culture period in the 85 : 15 PLGA, significantly. We also found that the numbers of cells were low in middle portion of scaffolds, and in large pore-sized group of 85 : 15 PLGA, there were many cells in the lower portion of the scaffolds more than that of small pore group. In conclusion, the pore size of the scaffold for chondrocyte culture is important for cell migration and proliferation, and PLGA, especially 85 : 15 PLGA with 300- 350 micrometer sized pore is the more suitable biomatrix for proliferation and migration of the chondrocytes.
Cell Movement
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Chondrocytes*
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DNA
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Porifera
9.Isolation and Structure Determination of an Imidazo-pyrimidine, 5-Chlorocavernicolin, Maleimide oximes and Nucleosides from a Marine Sponge Extract.
Roshan R KULKARNI ; Jang Hoon KIM ; Young Ho KIM ; Sangtaek OH ; Minkyun NA
Natural Product Sciences 2015;21(1):25-29
In a continuation of our studies to discover bioactive secondary metabolites from marine sources, we further investigated samples from a tryptamine and phenyl-alkane producing sponge, which resulted in the isolation of four uncommon small molecules and five nucleosides. Their structures were determined to be 7,8-dihydroimidazo[1,5-c]pyrimidin-5(6H)-one (1), 5-chlorocavernicolin (2), maleimide-5-oxime (3), 3-methylmaleimide-5-oxime (4), uridine (5), 2'-deoxyuridine (6), thymidine (7), adenine (8), and adenosine (9) by spectroscopic analyses. The isolated compounds were evaluated for inhibitory activity against soluble epoxide hydrolase (sEH) as well as the Wnt/beta-catenine signaling pathway.
Adenine
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Adenosine
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Nucleosides*
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Oximes*
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Porifera*
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Thymidine
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Uridine
10.Marine Sponges as a Drug Treasure.
Komal ANJUM ; Syed Qamar ABBAS ; Sayed Asmat Ali SHAH ; Najeeb AKHTER ; Sundas BATOOL ; Syed Shams ul HASSAN
Biomolecules & Therapeutics 2016;24(4):347-362
Marine sponges have been considered as a drug treasure house with respect to great potential regarding their secondary metabolites. Most of the studies have been conducted on sponge's derived compounds to examine its pharmacological properties. Such compounds proved to have antibacterial, antiviral, antifungal, antimalarial, antitumor, immunosuppressive, and cardiovascular activity. Although, the mode of action of many compounds by which they interfere with human pathogenesis have not been clear till now, in this review not only the capability of the medicinal substances have been examined in vitro and in vivo against serious pathogenic microbes but, the mode of actions of medicinal compounds were explained with diagrammatic illustrations. This knowledge is one of the basic components to be known especially for transforming medicinal molecules to medicines. Sponges produce a different kind of chemical substances with numerous carbon skeletons, which have been found to be the main component interfering with human pathogenesis at different sites. The fact that different diseases have the capability to fight at different sites inside the body can increase the chances to produce targeted medicines.
Carbon
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Humans
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In Vitro Techniques
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Pharmacokinetics
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Porifera*
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Skeleton