OBJECTIVETo study the chemical constituents of the essential substance from the Polygonum oriental.

METHODChromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence.

RESULTSeven compounds were obtained and identified as ombuine-3-O-beta-D-galactopyranoside (1), ombuine-3-O-rutinoside (2), tryptophan (3), quercetin-3-O-methyl ether (4), kaempferol-3-O-(2"-O-alpha-L-rhamnopyranosyl) -beta-D-glucuronopyranoside (5), quercetin-3-O-(2"-O-alpha-L-rhamnopyranosyl)-beta-D-glucuronopyranoside (6), quercetin-3-O-beta-D-glucuronide (7).

CONCLUSIONCompounds 4-7 were isolated from P. oriental for the first time and compounds 1-3 were firstly obtained from the genus Polygonum. The total 1H and 13C-NMR date of compound 1 were assigned for first time.

Magnetic Resonance Spectroscopy ; Polygonum ; chemistry

To understand the distribution and accumulation rules of polydatin, resveratrol, anthraglycoside B, emodin and physicion in different tissue structure of rhizome and root of Polygonum cospidatum, the content of 5 active compounds were analyzed simultaneously by HPLC, based on plant anatomy and histochemistry. The rhizome and root consist of different tissues, with an increased diameter, the proportions of the secondary xylem and phloem have increased. Resveratrol and polydatin mainly distributed in the pith, the secondary phloem and periderm of rhizome, and the secondary phloem and periderm of the root, while emodin and anthraglycoside B concentrated in the secondary structure and pith of rhizome mostly. In different thickness of the measured samples, the total contents of 5 compounds were correspondingly higher in thinner rhizome and root than those in the coarse ones.
Plant Roots ; chemistry ; Polygonum ; chemistry ; Rhizome ; chemistry

OBJECTIVETo evaluate the quality of Polygonum perfoliatum collected from GAP planting base efficiently so as to provide scientific evidence for its GAP tending plant by HPLC fingerprinting method.

METHODAnalysis was performed on a reserved-phase column Lichrosher-C18 column (4.6 mm x 250 mm, 5 microm), A linear gradient elution of 0.2% aqueous acetic acid and acetonitrile was used for the separation.

RESULTWildlife tending P. perfoliatum was relatively stable. The chromatograms of the samples from 2011 were more accordant than those from 2010. Samples in Dafang were the most stable and had little differences during the two years. The quality of P. perfoliatum planted in the regions of sample GBG-(GD) 002 in Guiding and sample GBG-(LL) 003 in Longli were more stable and better than those from other regions.

CONCLUSIONIt is a feasible method that can be used to evaluate the quality of P. perfoliatum for GAP planting.

Chromatography, High Pressure Liquid ; methods ; Polygonum ; chemistry

OBJECTIVETo explore the character of inorganic elements in Polygonum multiflorum.

METHODThe contents of elements such as Al, B, Ba, Ca, Cu, Mn, Mg, Fe, Na, Ni, P, Se, Sr, Ti and Zn in nine P. multiflorum samples were determined by means of ICP-AEC. The results were used for the development of element distrubution diagram. The principal component analysis and one-way ANOVA of SPSS were applied for the study of characteristic elements in P. multiflorum.

RESULTThe contents of Al, Ca, K, Mg, Sr,Ti in wild P. multiflorum were remarkable higher than those in cultured P. multiflorum, and there was no significant difference between cultured and wild in the other elements. Five principal components which accounted for over 90% of the total variance were extracted from the original data. The analysis results show that Al, B, Ba, Fe, Na, Ni, Ti, Ca and Sr may be the characteristic elements in P. multiflorum. The element distrubution diagram of the sample from Tianyang was remarkable different comparing with the others.

CONCLUSIONThe principal component analysis could be used in data processing in inorganic elements.

Elements ; Polygonum ; chemistry ; Principal Component Analysis ; methods

OBJECTIVETo investigate the chemical constituents in herbs of Polygonum jucundum.

METHODThe 85% ethanol extract was separated by means of silica gel and Sephadex LH-20 column chromatography. The compounds isolated from the plant were identified by physicochemical properties and spectroscopic evidence.

RESULTEight compounds were isolated and identified as: quercetin-3'-O-beta-D-galactoside (1), 8-methoxyquercetin (2), pigenin (3), luteolin (4), quercetin (5), 3,5,7-trihydroxychromone (6), p-hydroxybenzaldehyde (7), beta-sitosterol (8).

CONCLUSIONAll compounds were isolated from this plant for the first time, compounds 5- 8 were isolated from the genus Polygonum for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Polygonum ; chemistry

OBJECTIVETo study the chemical constituents in the active portion from the flowers of Polygonum oriental.

METHODChromatographic techniques were employed for isolation and purification of the constituents and their structures were determined by spectral analysis and chemical evidence.

RESULTNine compounds were obtained and identified as alphitonin (1), methyl 3, 4-dihydroxybenzoate (2), apocynin (3), kaempferol-3-O-beta-D-glucoside (4), 1,3,5-trihydroxybenzene (5), 3,3'-dimethoxyellagic-acid-4-O-beta-D-glucoside (6), kaempferol-3-O-alpha-L-rhamnoside (7), quercetin-3-O-alpha-L-rhamnoside (8), kaempferol (9).

CONCLUSIONCompounds 2, 4, 5 were isolated from P. oriental for the first time and compounds 1, 3 were firstly obtained from the genus Polygonum.

Drugs, Chinese Herbal ; analysis ; Flowers ; chemistry ; Polygonum ; chemistry

To establish a UPLC method for simultaneous determination of seven chemical markers (gallic acid, catechin, alphitonin, taxifolin, kaempferol-3-O-beta-D-glucoside, quercetin-3-O-alpha-L-rhamnoside, and kaempferol-3-O-alpha-L-rhamnoside) of the flowers of Polygonum Oriental collected from Guizhou province. Separation was performed at 45 degrees on a ACQUITY UPLC BEH C18 column (2.1 mm x 150 mm, 1.7 microm) with a gradient solvent system of 0.1% formic acid in acetonitrile-0.1% aqueous formic acid as the mobile phase. The flow rate was 0.3 mL x min(-1), the detection wavelength was 280 nm, and the sample injection volume was 1 microL. In the selected linear range, all calibration curves of the seven chemical markers showed good linearity. The recoveries (n = 6) were in the range of 97.90% -101.4% and RSD were less than 3.8%. The results showed that the seven compounds contained in all the samples of the flowers of P. orientale can be chosen as their chemical markers and that this UPLC-PAD method is simple, reliable and suitable for the quality control of the flowers of P. orientale. However, the chemical markers may be present at different levels for samples collected in different areas and at different times. A collection time of mid-to-late August with relatively high content of the chemical markers was suggested for the flowers of P. orientale.
Chromatography, Liquid ; instrumentation ; methods ; Flowers ; chemistry ; Lasers, Semiconductor ; Polygonum ; chemistry

Several kinds of column chromatography methods were used to investigate the chemical constituents of roots of Polygonum multiflorum. The structures of the isolated compounds were identified based on their physicochemical properties, spectral data and chemical methods. A new chromone glycoside was isolated and its structure was identified as (S)-2-(2'-hydroxypropyl)-5-methyl7-hydroxychromone-7-0-alpha-L-fucopyranosyl (1-->2)-beta-D-glucopyranoside (1).
Drugs, Chinese Herbal ; chemistry ; Glycosides ; chemistry ; Molecular Structure ; Plant Roots ; chemistry ; Polygonum ; chemistry

OBJECTIVETo establish a near-infrared qualitative analysis model to identify the authenticity of Polygonum multiflo- rum and distinguish processed products Polygoni Multiflori Radix.

METHODThe NIR spectra were peformed on over 30 batches of P. multiflorum and Polygoni Multiflori Radix samples and the adulterants Cynanchum bungei, Pteroxygonum giraldii, Polygonum cillinerve to establish the qualitative discriminant model and the conformity test model of Polygonum multifiorum , and cluster analysis was used to analyze the samples from different origins.

RESULTThe model is able to identify correctly P. multiflorum with its counterfeit, and distinguish between P. multiflorum and Polygoni multiflori Radix.

CONCLUSIONNear-infrared spectroscopy can be applied in the identification of P. multiflorum, which could be used to screen Chinese herbal medicine preliminarily.

Drugs, Chinese Herbal ; chemistry ; Plant Roots ; chemistry ; Polygonum ; chemistry ; Spectroscopy, Near-Infrared ; methods

OBJECTIVETo study the technical conditions of the extraction and purification of the active composition from Polygonum orientale and Crataegus pinnatifida Bge. in Hongye Xingtong soft capsules with the macroporous resin.

METHODThe orientm, isorientm and hyperoside were used as index to screen the five kinds of resins. And the technical conditions of the enrichment and purification of D101 resin selected out of above were all-round studied.

RESULTThe D101 was fit for adsorbing orientm, isorientm and hyperoside. Under the optimal conditions, the transfer rate of orientm, isorientm and hyperoside was above 91%, and the total solid was cut down by more than 60%.

CONCLUSIONThe D101 is greatly effective for the enrichment and purification of the active composition of P. orientale and C. pinnatifida Bge.

Crataegus ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Polygonum ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; isolation & purification ; Resins, Synthetic ; chemistry