1.Detection of Extracellular Enzyme Activity in Penicillium using Chromogenic Media.
Ji Hwan YOON ; Seung Beom HONG ; Seung Ju KO ; Seong Hwan KIM
Mycobiology 2007;35(3):166-169
A total of 106 Penicillium species were tested to examine their ability of degrading cellobiose, pectin and xylan. The activity of beta-glucosidase was generally strong in all the Penicillium species tested. P. citrinum, P. charlesii, P. manginii and P. aurantiacum showed the higher ability of producing beta-glucosidase than other tested species. Pectinase activity was detected in 24 Penicillium species. P. paracanescens, P. sizovae, P. sartoryi, P. chrysogenum, and P. claviforme showed strong pectinase activity. In xylanase assay, 84 Penicillium species showed activity. Strong xylanase activity was detected from P. megasporum, P. sartoryi, P. chrysogenum, P. glandicola, P. discolor, and P. coprophilum. Overall, most of the Penicillium species tested showed strong beta-glucosidase activity. The degree of pectinase and xylanase activity varied depending on Penicillium species.
beta-Glucosidase
;
Cellobiose
;
Penicillium*
;
Polygalacturonase
2.Detection of Extracellular Enzyme Activities in Ganoderma neo-japonicum.
Woo Sik JO ; Ha Na PARK ; Doo Hyun CHO ; Young Bok YOO ; Seung Chun PARK
Mycobiology 2011;39(2):118-120
The ability of Ganoderma to produce extracellular enzymes, including beta-glucosidase, cellulase, avicelase, pectinase, xylanase, protease, amylase, and ligninase was tested in chromogenic media. beta-glucosidase showed the highest activity, among the eight tested enzymes. In particular, Ganoderma neo-japonicum showed significantly stronger activity for beta-glucosidase than that of the other enzymes. Two Ganoderma lucidum isolates showed moderate activity for avicelase; however, Ganoderma neo-japonicum showed the strongest activity. Moderate ligninase activity was only observed in Ganoderma neo-japonicum. In contrast, pectinase, amylase, protease, and cellulase were not present in Ganoderma. The results show that the degree of activity of the tested enzymes varied depending on the Ganoderma species tested.
Amylases
;
beta-Glucosidase
;
Cellulase
;
Cellulases
;
Ganoderma
;
Oxygenases
;
Polygalacturonase
;
Reishi
3.Detection of Extracellular enzymes Activities in Various Fusarium spp..
Hyuk Woo KWON ; Ji Hwan YOON ; Seong Hwan KIM ; Seung Beom HONG ; Youngah CHEON ; Seung Ju KO
Mycobiology 2007;35(3):162-165
Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested species Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum showed high beta-glucosidase acitivity. Xylanase activity was strongly detected in F. proliferatum and F. oxysporum. Strong pectinase activity was also found in F. oxysporum and F. proliferatum. Amylase activity was apparent in F. oxysporum. No clear activity in cellulase was found from all the Fusarium species tested.
Amylases
;
beta-Glucosidase
;
Cellobiose
;
Cellulase
;
Fusarium*
;
Polygalacturonase
;
Starch
4.Extracellular Enzyme Activities of the Monokaryotic Strains Generated from Basidiospores of Shiitake Mushroom.
Hyuk Woo KWON ; In Joung BACK ; Han Gyu KO ; Chang Hyun YOU ; Seong Hwan KIM
Mycobiology 2008;36(1):74-76
To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, beta-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.
Amylases
;
beta-Glucosidase
;
Cellulases
;
Humans
;
Parents
;
Polygalacturonase
;
Shiitake Mushrooms
5.Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus).
Hyuk Woo KWON ; Min Ah CHOI ; Yeo Hong YUN ; Youn Lee OH ; Won Sik KONG ; Seong Hwan KIM
Mycobiology 2015;43(1):81-86
To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of IGS I, which ranged from 1,301 to 1,348 bp, were present among twenty ASI1346-derived progeny strains, while 15 different sizes of IGS I, which ranged from 700 to 1,347 bp, were present among twenty ASI1038-derived progeny strains. Phylogenetic analysis of the IGS sequences revealed that different clades were present in both the ASI10388- and ASI1346-derived progeny strains. Plating assays of seven kinds of extracellular enzymes (beta-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease) also revealed apparent variation in the ability to produce extracellular enzymes among the 40 tested progeny strains from both parental A. bisporus strains. Overall, this study demonstrates that characterization of IGS I regions and extracellular enzymes is useful for the assessment of the substrate-degrading ability and heterogenicity of A. bisporus monokaryotic strains.
Agaricales*
;
Amylases
;
Breeding
;
Cellulases
;
DNA, Ribosomal
;
Enzyme Assays
;
Humans
;
Parents
;
Polygalacturonase
6.Yeast Associated with the Ambrosia Beetle, Platypus koryoensis, the Pest of Oak Trees in Korea.
Yeo Hong YUN ; Dong Yeon SUH ; Hun Dal YOO ; Man Hwan OH ; Seong Hwan KIM
Mycobiology 2015;43(4):458-466
Oak tree death caused by symbiosis of an ambrosia beetle, Platypus koryoensis, and an ophiostomatoid filamentous fungus, Raffaelea quercus-mongolicae, has been a nationwide problem in Korea since 2004. In this study, we surveyed the yeast species associated with P. koryoensis to better understand the diversity of fungal associates of the beetle pest. In 2009, a total of 195 yeast isolates were sampled from larvae and adult beetles (female and male) of P. koryoensis in Cheonan, Goyang, and Paju; 8 species were identified by based on their morphological, biochemical and molecular analyses. Meyerozyma guilliermondii and Candida kashinagacola were found to be the two dominant species. Among the 8 species, Candida homilentoma was a newly recorded yeast species in Korea, and thus, its mycological characteristics were described. The P. koryoensis symbiont R. quercusmongolicae did not show extracelluar CM-cellulase, xylanase and avicelase activity that are responsible for degradation of wood structure; however, C. kashinagacola and M. guilliermondii did show the three extracellular enzymatic activities. Extracelluar CM-cellulase activity was also found in Ambrosiozyma sp., C. homilentoma, C. kashinagacola, and Candida sp. Extracelluar pectinase activity was detected in Ambrosiozyma sp., C. homilentoma, Candida sp., and M. guilliermondii. All the 8 yeast species displayed compatible relationships with R. quercus-mongolicae when they were co-cultivated on yeast extract-malt extract plates. Overall, our results demonstrated that P. koryoensis carries the yeast species as a symbiotic fungal associate. This is first report of yeast diversity associated with P. koryoensis.
Adult
;
Ambrosia*
;
Beetles*
;
Candida
;
Cellulases
;
Chungcheongnam-do
;
Fungi
;
Humans
;
Korea*
;
Larva
;
Platypus*
;
Polygalacturonase
;
Quercus*
;
Symbiosis
;
Wood
;
Yeasts*
7.In vivo/in vitro evaluation of Kangfuxin colon targeting capsules.
Xue-lan QIU ; Ming YANG ; Xing-liang XIE ; Juan LAI ; Si-wei CHEN
China Journal of Chinese Materia Medica 2007;32(16):1644-1647
OBJECTIVETo evaluate the release in fixed position of pH-dependent and enzyme-dependent Kangfuxin colon targeting capsules in vivo and in vitro.
METHODThe dissolution was tested in vitro and X-ray radiography was used for the evaluation in vivo.
RESULTAfter two hours pH-dependent colon targeting in man-made colon fluid, medicine release in fixed position on the whole, colon loc-release. Add enzyme into man-made colon, when enzyme-dependent colon targeting in it, then medicine release quickly, mainly release in fixed position; The conveying time in vivo of pH-dependent and enzyme-dependent capsules have big individuality difference. In the experiment, disintegration is stabilize among individuales, between 2.0-3.5 hours.
CONCLUSIONKangfuxin colon targeting capsules of two principles all release in fixed position to achieve the goal.
Animals ; Capsules ; Colon ; diagnostic imaging ; metabolism ; Delayed-Action Preparations ; chemistry ; Drug Carriers ; Drug Delivery Systems ; Female ; Humans ; Hydrogen-Ion Concentration ; Male ; Materia Medica ; administration & dosage ; isolation & purification ; pharmacokinetics ; Periplaneta ; chemistry ; Polygalacturonase ; chemistry ; Radiography
8.Expression of endopolygalacturonase A of Aspergillus oryzae in Escherichia coli.
Yu-Ling ZHANG ; Qing-Xin ZHAO ; Hong ZHU ; Jing SUN ; Feng-Min HAN ; Sheng YUAN
Chinese Journal of Biotechnology 2007;23(1):101-105
Pectinases are mainly used in the food industry to clarify fruit juices and wine, improve oil extraction, remove the peel from the citrus fruit, increase the firmness of some fruits and degum fibres. The filamentous fungus Aspergillus oryzae, used for the production of traditional fermented foods, only could produce less pectinases under general conditions. So far only a few of PGs expressed in yeast or E. coli were reported but they did not show higher activity. The cDNA of mature PGA (without signal peptide) was synthesized with specific primers from total RNA of Aspergillus oryzae by RT-PCR. PGA cDNA was ligated into pET-28a( + ) expression vector, creating plasmid pET-28a( + )-pgA. The plasmid pET-28a( + )-pgA was transformed into E. coli Turner (DE3) plac I cells to express PGA heterogeneously. For improving the efficiency of PGA expression in E. coli, the conditions for expression of the PGA in E. coli were optimized. E. coli Turner (DE3) plac I cells with pET-28a( + )-pgA was first cultivated at 37 degrees, 220r/min until OD600nm reached about 0.8. Then, cultivation broth was added with 0.5 mmol/L IPTG and incubated at 15 degrees C, 170r/min for other 24 h for induced-expression of PGA. Our data showed that the activity of recombinant expressed PGA could reach to 70u/mL medium, which is 87.5-fold of the activity of PGA produced in culture of A. oryzae and superior than known recombinant expression amount of PGA reported by other researchers.
Amino Acid Sequence
;
Aspergillus oryzae
;
enzymology
;
genetics
;
Base Sequence
;
Biocatalysis
;
DNA, Complementary
;
genetics
;
Escherichia coli
;
genetics
;
Fungal Proteins
;
genetics
;
metabolism
;
Gene Expression Regulation, Enzymologic
;
Gene Expression Regulation, Fungal
;
Molecular Sequence Data
;
Plasmids
;
genetics
;
Polygalacturonase
;
genetics
;
metabolism
;
Recombinant Proteins
;
metabolism
;
Sequence Homology, Amino Acid
;
Sequence Homology, Nucleic Acid
;
Temperature
;
Time Factors
9.A simplified and miniaturized glucometer-based assay for the detection of β-glucosidase activity.
Min-Yi JIN ; Tong ZHANG ; Yi-Shun YANG ; Yue DING ; Jun-Song LI ; Gao-Ren ZHONG
Journal of Zhejiang University. Science. B 2019;20(3):264-272
β-Glucosidase activity assays constitute an important indicator for the early diagnosis of neonatal necrotizing enterocolitis and qualitative changes in medicinal plants. The drawbacks of the existing methods are high consumption of both time and reagents, complexity in operation, and requirement of expensive instruments and highly trained personnel. The present study provides a simplified, highly selective, and miniaturized glucometer-based strategy for the detection of β-glucosidase activity. Single-factor experiments showed that optimum β-glucosidase activity was exhibited at 50 °C and pH 5.0 in a citric acid-sodium citrate buffer when reacting with 0.03 g/mL salicin for 30 min. The procedure for detection was simplified without the need of a chromogenic reaction. Validation of the analytical method demonstrated that the accuracy, precision, repeatability, stability, and durability were good. The linear ranges of β-glucosidase in a buffer solution and rat serum were 0.0873-1.5498 U/mL and 0.4076-2.9019 U/mL, respectively. The proposed method was free from interference from β-dextranase, snailase, β-galactosidase, hemicellulase, and glucuronic acid released by baicalin. This demonstrated that the proposed assay had a higher selectivity than the conventional dinitrosalicylic acid (DNS) assay because of the specificity for salicin and unique recognition of glucose by a personal glucose meter. Miniaturization of the method resulted in a microassay for β-glucosidase activity. The easy-to-operate method was successfully used to detect a series of β-glucosidases extracted from bitter almonds and cultured by Aspergillus niger. In addition, the simplified and miniaturized glucometer-based assay has potential application in the point-of-care testing of β-glucosidase in many fields, including medical diagnostics, food safety, and environmental monitoring.
Animals
;
Aspergillus niger
;
Calibration
;
Cellulase/analysis*
;
Chemistry, Clinical/methods*
;
Dextranase/analysis*
;
Enterocolitis, Necrotizing/diagnosis*
;
Equipment Design
;
Flavonoids/analysis*
;
Glucose/analysis*
;
Glucuronic Acid/analysis*
;
Glucuronidase/analysis*
;
Glycoside Hydrolases/analysis*
;
Hydrogen-Ion Concentration
;
Linear Models
;
Multienzyme Complexes/analysis*
;
Plants, Medicinal
;
Polygalacturonase/analysis*
;
Rats
;
Reproducibility of Results
;
beta-Galactosidase/analysis*
;
beta-Glucosidase/analysis*