1.Application of cutinase in the degradation of biodegradable polyester poly(butylene adipate-co-terephthalate).
Hui WANG ; Jing WU ; Sheng CHEN ; Wei XIA
Chinese Journal of Biotechnology 2023;39(5):1987-1997
With the environmental pollution caused by waste plastics becoming increasingly serious, biodegradable polyester has become the focus of public attention. Poly(butylene adipate-co-terephthalate) (PBAT) is a biodegradable polyester formed by the copolymerization of aliphatic and aromatic groups, which has excellent performance of both. The degradation of PBAT under natural conditions requires strict environmental conditions and long degradation cycle. To address these shortcomings, this study explored the application of cutinase in PBAT degradation and the impact of butylene terephthalate (BT) content on the biodegradability of PBAT, so as to improve the degradation rate of PBAT. Five Polyester degrading enzymes from different sources were selected to degrade PBAT to pick out the most efficient enzyme. Subsequently, the degradation rate of PBAT materials with different BT content were determined and compared. The results showed that cutinase ICCG was the best enzyme for PBAT biodegradation, and the higher the BT content, the lower the degradation rate of PBAT. Furthermore, the optimum temperature, buffer type, pH, the ratio of enzyme to substrate (E/S) and substrate concentration in the degradation system were determined to be 75 ℃, Tris HCl, 9.0, 0.4% and 1.0% respectively. These findings may facilitate the application of cutinase in PBAT degradation.
Polyesters/chemistry*
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Adipates
2.Study on degradation performance of star-shaped polymer of ESO and lactide.
Bei ZHANG ; Fu CHUNHUA ; Wang YUANLIANG
Journal of Biomedical Engineering 2011;28(3):517-542
Star-shaped polymer of epoxidized soybean oil (ESO) and lactide is a new material. Its degradation function has an importance to its applications. There are many factors affecting its degradation. This paper is mainly aimed at investigation in which the surface morphology, pH, molecular weight and weight loss rate, branching ESO-lactide star polymers the degree of change were studied. It was found through the experiments that, the changing rates of the branching ESO-lactide star polymers were slower than the degree of PDLLA change compared to the surface morphology, pH, molecular weight and weight loss rate. This has a guiding significance to the further application of ESO-lactide.
Polyesters
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chemistry
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Polymers
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chemistry
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Soybean Oil
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chemistry
3.Recent advances in nucleating agents used for poly (lactic acid).
Zhiyang LIU ; Yunxuan WENG ; Zhigang HUANG ; Nan YANG ; Min ZHANG
Chinese Journal of Biotechnology 2016;32(6):798-806
Poly (lactic acid) (PLA) is a polymer synthesized from lactic acid with good biocompatibility and biodegradability. At present, PLA manufactured on industrial scale is mainly synthesized from L-lactic acid. The obtained products have good transparency but poor heat resistance. Adding nucleating agents could increase the crystallinity of PLA, to improve heat resistance. We reviewed the progress of research on organic and inorganic nucleating agents that can be used for PLA synthesis.
Chemical Industry
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Lactic Acid
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Polyesters
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chemistry
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Polymers
4.Research on Chemical Equivalence Characteristics of Polylactic Acid in Medical Devices.
Jing QIN ; Yahong KANG ; Yong SHEN ; Xin SONG ; Hongyu LUO ; Hongyan JIANG
Chinese Journal of Medical Instrumentation 2018;42(4):246-249
Polylactic acid is synthesized indirectly by the polymerization method, according to the standard GB/T 16886.18-2011, the evaluation parameters and methods about chemical characterization of polylactic acid have been established. By using rigorous and comprehensive comparative analysis, the chemical equivalency of domestic and imported polylactic acid materials has been proved, along with the "Medical Device Biology Evaluation and Review Guide", paving the way of using domestic polylactic acid in implantable medical devices.
Equipment and Supplies
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Lactic Acid
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Polyesters
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chemistry
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Polymers
5.Studies on a kind of new biodegradable material--polycaprolactone and developments in medical area.
Journal of Biomedical Engineering 2003;20(4):746-749
Nowadays, there are more and more researches on characters and applications of polycaprolactone (PCL). This paper reviews the researches of polycaprolactone, including its synthesis, physical and chemical properties, biodegradation, absorption properties, and its applications in clinical use and drug delivery systems. PCL can be from open ring polymerizaion. It is a kind of semi-crystalline polymer, and has good flexibility. The degradation reaction of PCL is hydrolysis. PCL is not cumulated in the body, and can be totally excreted. It can be used as suture, material for fixation of bone fracture, vector of drug, etc. PCL is widely used in medical area because of its safety, low toxicity, biodegradability.
Biodegradation, Environmental
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Drug Carriers
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Polyesters
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chemical synthesis
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chemistry
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metabolism
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Sutures
6.Processing, properties and application of poly lactic acid (PLA) fiber.
Shenyang CAI ; Guang HU ; Jie REN
Chinese Journal of Biotechnology 2016;32(6):786-797
Poly lactic acid (PLA) fibers a biodegradable fiber produced from PLA resin by melt spinning, solvent spinning or electrostatic spinning. Based on the excellent safety, comfortability, environmental protection and good mechanical properties, PLA can be widely applied in textile fabric, nonwoven, filler fabric and many downstream health products application, such as sanitary napkins, baby diapers, facial masks, and wipes.
Biotechnology
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Chemical Industry
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Lactic Acid
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Polyesters
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chemistry
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Polymers
7.Biomanufactured polyhydroxyalkanoates (PHA) modification: a review.
Yingxin ZHOU ; Nan YANG ; Xiyuan WANG ; Yunxuan WENG ; Xiaoqian DIAO ; Min ZHANG ; Yujuan JIN
Chinese Journal of Biotechnology 2016;32(6):738-747
In this review, we presented the industrial status of biomanufactured polyhydroxyalkanoates (PHA), including poly (3-hydroxybutyrate) (PHB), poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), poly (3-hydroxybutyrate-co-4-hydroxybutyrate) (P3/4HB)), and poly (3-hydroxybutyrate-3-hydroxycaproate) (PHBH). A lot of modification studies, aimed at solving problems of poor thermal stability, narrow processing window and other drawbacks of PHA, are discussed. The properties of PHA can be optimized by using proper modification method, in order to expand its applications.
3-Hydroxybutyric Acid
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Biotechnology
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Hydroxybutyrates
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Polyesters
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Polyhydroxyalkanoates
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chemistry
8.Preparation and biological evaluation of Chitin short fiber reinforced polycaprolactone composite.
Liang DUAN ; Zhifei XU ; Kang SUN ; Xuewei ZHAO ; Jia FANG ; Xiong QIN ; Zhiyun GONG
Journal of Biomedical Engineering 2006;23(3):565-568
Chitin short fiber reinforced polycaprolactone composite was prepared by melting blending method. The cytotoxicity and biocompatibility of pure polycaprolactone and of chitin short fiber reinforced polycaprolactone composite were investigated in order to provide useful scientific basis for clinical application. The biocompatibility of pure polycaprolactone and that of chitin short fiber reinforced polycaprolactone composite were evaluated by a series of tests, including cytotoxicity test in vitro, acute systemic toxicity test, hemolysis test, pyrogen test and sensitivity test. The results showed that the cytotoxicity scores of the two materials were grade 0 and the growth and proliferation of the cultured cells were not significantly inhibited by the two materials. There were no potential allergic materials in the composites and the maceration extract showed no hemolytic reaction, no acute systemic toxicity and no pyrogen reaction. We conclude that the composites have fine biocompatibility and are safe for clinical use in the reconstruction treatment for chest wall defect.
Biocompatible Materials
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chemistry
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Bone Substitutes
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chemistry
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Chitin
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chemistry
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Materials Testing
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Polyesters
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chemistry
9.Preparation of sustained release microspheres containing extracts from Caulis sinomenii with polylactic acid and their release property in vitro.
China Journal of Chinese Materia Medica 2010;35(16):2142-2145
OBJECTIVETo prepare polylactic acid microspheres containing total alkaloid extracts of Caulis sinomenii and study their release characteristics in vitro.
METHODPolylactic acid microspheres containing total alkaloid extracts of C. sinomenii were prepared by O/W emulsification solvent-evaporation process. The microspheres were characterized in terms of morphology, encapsulation efficiency, and particle size distribution. The effect of different conditions on release property of microspheres was studied.
RESULTThe formed microspheres were spherical with smooth surfaces. The encapsulation efficiency and rate of drug loading were (83.4 +/- 5.63)% and (8.7-0.35)%, respectively. The distribution of particle size was uniform and the average size was (21.5 +/- 1.22) microm. In vitro release study revealed that the 32-hour accumulative release percentage reached 80%.
CONCLUSIONPolylactic acid microspheres containing total alkaloid extracts of C. sinomenii were prepared successfully. Microspheres with good sustained-release characteristics can be produced by controlling different process parameters.
Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Lactic Acid ; chemistry ; Microspheres ; Polyesters ; Polymers ; chemistry ; Sinomenium ; chemistry
10.In vivo degradation and tissue compatibility of polyphosphazene blend films.
Journal of Biomedical Engineering 2002;19(2):191-195
The degradation performance and tissue compatibility of polyphosphazene/polyester or polyanhydride (PGP/PLGA or PGP/PSTP) blend films were investigated in mice. The results showed that the blend degradation rate could be regulated by adjusting PLGA or PSTP content in the blends. For example, the weight loss of PGP/PLGA (70:30 by wt) film was 68.4% after 55 days, whereas that of PGP/PSTP (70:30 by wt) was 88.5% only after 12 days. Also the mechanisms for in vivo degradation of blends of PGP/PLGA and PGP/PSTP were hydrolysed enzymatically and non-enzymatically, respectively. The tissue compatibility of PGP/PLGA was better than that of PGP/PSTP, and an increase of the percentage of PGP in PGP/PSTP blends would help improve the tissue compatibility. These findings suggested that polyphosphazene blends be potential materials applied in medical field.
Animals
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Biocompatible Materials
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chemistry
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Mice
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Organophosphorus Compounds
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chemistry
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Polyesters
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chemistry
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Polymers
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chemistry