OBJECTIVETo investigate the aporphine alkaloids in the branches and leaves of Polyalthia nemoralis.

METHODThe compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.

RESULTFive aporphine alkaloids were isolated and identified as: bidebiline A (1), annobraine (2), lanuginosine (3), liriodenine (4), oxostephanosine (5), respectively.

CONCLUSIONFor the first time, Compounds 2 and 5 were obtained from Polyalthia while 1, 3 and 4 isolated from this plant. The bioassays in vitro against five human tumor cell lines with MTT method showed moderate cytotoxic activities (IC50 1 mg x L(-1)) of compounds 3-5.

Antineoplastic Agents, Phytogenic ; chemistry ; isolation & purification ; pharmacology ; Aporphines ; chemistry ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Humans ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Polyalthia ; chemistry

OBJECTIVETo investigate the active constituents of the branches and leaves of Polyalthia nemoralis.

METHODThe compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.

RESULTEight compounds were isolated and identified as: zincpolyanemin (1), nickel bis-(pyridine-N-oxide-2-thiolate) (2), cupric bis (pyridine-N-oxide-2-thiolate (3), 2-methanesulfonyl-pyridine (4), 2-pyridinethiolate N-oxide (5), 2,2'-dithiodipyridine (6), 2-thiohydroxypyridin-N-oxide-2-S-beta-D-gluco pyranoside (7) and pyridine-N-oxide (8), respectively.

CONCLUSIONCompounds 2, 4-6, 8 were new natural products. The bioassays in vitro against five human tumor cell lines with MTT method showed stronger cytotoxic activities (IC50 0.05-0.09 mg x L(-1)) for compounds 1-3 and 6, and weaker cytotoxic activities (IC50 5.49-7.71 mg x L(-1)) for compound 5.

Cell Line, Tumor ; Cell Survival ; drug effects ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Magnetic Resonance Spectroscopy ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Polyalthia ; chemistry ; Pyridines ; chemistry

OBJECTIVETo investigate the chemical constituents of the branches and leaves of Polyalthia nemoralis.

METHODThe compounds were isolated and purified by silica gel, macroporous adsorption resin and Sephadex LH-20 column chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data.

RESULTFourteen compounds were isolated and identified as syringic acid (1), 3-methoxy-4-hydroxycinnamic acid (2), vanillic acid (3), 4-hydroxybenzoic acid (4), mauritianin (5), (+)-xylopinidine (6), (+)-oblongine(7), (+)-tembetarine (8), eythritol (9), D-mannitol (10), ethyl-beta-D-glucopyranoside (11), (+)-magnoflorine (12), stepharanine (13), (2S, 4R)-4-hydroxy-2-piperidine-carboxylic acid (14), respectively.

CONCLUSIONAll the compounds were isolated from the genus Polyalthia for the first time; compounds 6 and 13 showed inhibitation activities against multi tumor cell lines.

Alkaloids ; chemistry ; isolation & purification ; pharmacology ; Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Aporphines ; chemistry ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Survival ; drug effects ; Chromatography, Agarose ; methods ; Coumaric Acids ; chemistry ; isolation & purification ; pharmacology ; Gallic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology ; Humans ; Kaempferols ; chemistry ; isolation & purification ; pharmacology ; Parabens ; chemistry ; isolation & purification ; pharmacology ; Plant Extracts ; chemistry ; isolation & purification ; pharmacology ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Polyalthia ; chemistry ; Vanillic Acid ; chemistry ; isolation & purification ; pharmacology