1.Advances in innate immune responses induced by Mycoplasma hyopneumoniae infection.
Jiacui LAI ; Jiawei HE ; Honglei DING
Chinese Journal of Biotechnology 2023;39(12):4773-4783
Mycoplasma hyopneumoniae is the pathogen causing swine mycoplasmal pneumonia. The lack of well-established animal models of M. hyopneumoniae infection has delayed the progress of M. hyopneumoniae-related anti-infection immunity studies. This paper reviews the inflammatory response, the recognition of M. hyopneumoniae by the innate immune system, and the role of innate immune cells, complement system, antimicrobial peptides, autophagy, and apoptosis in M. hyopneumoniae infection. The aim was to elucidate the important roles played by the components of the innate immune system in the control of M. hyopneumoniae infection, and prospect key research directions of innate immune response of M. hyopneumoniae infection in the future.
Animals
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Swine
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Mycoplasma hyopneumoniae
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Pneumonia of Swine, Mycoplasmal
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Immunity, Innate
2.A sensitive and specific polymerase chain reaction to detect Mycoplasma hyopnemoniae using Mycoplasma protein P97 gene.
Sunhwa HONG ; Sang Ho PARK ; Yun Seong LEE ; Okjin KIM
Journal of Biomedical Research 2013;14(3):160-164
Mycoplasma (M.) hyopneumoniae is the causative agent of swine enzootic pneumonia, a disease that is prevalent in every country where pigs are raised. In this study, we aimed to develop a sensitive and specific PCR assay to detect M. hyopneumoniae in pigs. The suitability of this PCR assay for the detection of mycoplasmal infection was also tested using clinical lung samples from slaughtered pigs. We developed a probe and M. hyopneumoniae-specific primer pairs, MhyoP-F and MhyoP-R, for the new PCR assay based on regions in the Mycoplasma protein P97 gene that are unique to M. hyopneumoniae. The developed PCR assay was very specific and sensitive for the detection of M. hyopneumoniae. The assay was able to detect the equivalent of 10 pg of target template DNA, which indicates that the assay was very sensitive. In addition, the M. hyopneumoniae PCR assay detected only M. hyopneumoniae and no other Mycoplasma spp. or bacterial species of another genera. Further, the newly developed PCR assay effectively detected M. hyopneumoniae infection in pigs. We suggest that this PCR assay using M. hyopneumoniae-specific primer pairs, MhyoP-F and MhyoP-R, will be useful and effective for monitoring M. hyopneumoniae infection in pigs.
Diagnosis
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DNA
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Lung
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Mycoplasma*
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Pneumonia of Swine, Mycoplasmal
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Polymerase Chain Reaction*
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Swine