The abnormality of platelet function plays an important role in the pathogenesis and evolution of blood stasis syndrome (BSS). The explanation of its mechanism is a key scientific issue in the study of cardiovascular and cerebrovascular diseases and treatment. System biology technology provides a good technical platform for further development of platelet multi-omics, which is conducive to the scientific interpretation of the biological mechanism of BSS. The article summarized the pathogenesis of platelets in BSS, the mechanism of action of blood activating and stasis resolving drugs, and the application of genomics, proteomics, and metabonomics in platelet research, and put forward the concept of "plateletomics in BSS". Through the combination and cross-validation of multi-omics technology, it mainly focuses on the clinical and basic research of cardiovascular and cerebrovascular diseases; through the interactive verification of multi-omics technology and system biology, it mainly focuses on the platelet function and secretion system. The article systematically explains the molecular biological mechanism of platelet activation, aggregation, release, and other stages in the formation and development of BSS, and provides a new research idea and method for clarifying the pathogenesis of BSS and the mechanism of action of blood activating and stasis resolving drugs.
Blood Platelets ; Hemostasis ; Platelet Activation ; Proteomics ; Technology

No abstract available.
Blood Platelets* ; Disseminated Intravascular Coagulation* ; Platelet Activation*

BACKGROUND: We evaluate the efficacy of ultra-hydrophilic coated bypass circuits in comparison with uncoated bypass circuits in a porcine cardiopulmonary bypass model. MATERIAL AND METHOD: Normothermic cardiopulmonary bypass was performed in 10 anesthetized pigs via the left atrium and ascending aorta with a centrifugal biopump. Ultra-hydrophilic coated bypass circuits were used in 5 pigs (the study group) and uncoated bypass circuits were used for the control group. Platelet counts and platelet aggregation tests were performed. The thrombin-antithrombin (TAT) complex level and total protein level were evaluated. RESULT: There were no significant changes in the platelet counts and aggregation ability of both groups. The TAT complex levels were not different between the two groups. The total protein level was significantly lower in the control group after cessation of cardiopulmonary bypass. CONCLUSION: The clinical effects of ultra-hydrophilic coating circuits were not remarkable, in terms of reducing inflammatory reaction and protection of platelet function. However, the effect of protection for blood protein adsorption might be acceptable.
Adsorption* ; Aorta ; Blood Platelets* ; Cardiopulmonary Bypass ; Heart Atria ; Platelet Activation* ; Platelet Aggregation ; Platelet Count ; Swine

OBJECTIVE: Decrease of platelet density by degranulation of activated platelet is well correlated with decrease of mean platelet component (MPC) value. We intended to investigate the change of MPC, mean platelet volume, and platelet count according to the stroke stage and difference between ischemic and hemorrhagic infarction. METHOD: Thirty eight patients (ischemic stroke 28 men, hemorrhagic stroke 10 men) and twenty age-matched healthy persons were included in this study. They were divided into acute stage group and subacute stage group. Each of them were sampled by venously and investigated about mean platelet component, mean platelet volume, and platelet counts. RESULTS: In ischemic stroke, there was statically (p <0.05) meaningful decrease of MPC value in acute stage (27.5+/-1.7) compared to control group (28.8+/-0.9). And MPC value in subacute stage showed meaningful increase (28.1+/-1.3) compared to acute stage but still remained in statically lower value compared to control value. In hemorrhagic stroke, there was no meaningful difference of MPC value in acute stage group (28.6+/-2.0) and subacute stage group (27.9+/-1.1) compared to control group. CONCLUSION: In ischemic stroke patients, MPC value in acute stage decreased meaningfully and this change might be useful as a landmark in predicting the activity of infarction.
Blood Platelets* ; Humans ; Infarction ; Male ; Mean Platelet Volume ; Platelet Activation ; Platelet Count ; Stroke*

Platelets play a key role in the pathogenesis of atherothrombosis, and also perform the physiological hemostasis. The platelet function assays have values in the investigating patients with suspected platelet disorders and in studying the effects of anti-platelet drugs. There are increasing assays for investigating platelet function, including assessment of platelet adhesion, activation and aggregation, etc. However, all of these assays have certain limited sensitivity. It is necessary to develop a simple, sensitive assay that measures the activated platelet. This article reviewed advances of researches on platelet function assays, including assay for general function of platelet, assay of platelet adhesion function, platelet activation assay, platelet aggregation assay, platelet coagulation assay and application of flow cytometry in assessment of platelet functions, etc. and looked forward to research prospect in this field.
Blood Platelets ; physiology ; Humans ; Platelet Activation ; physiology ; Platelet Adhesiveness ; physiology ; Platelet Aggregation ; physiology ; Platelet Function Tests ; methods ; trends

OBJECTIVE: To study the effect of gradient shear stress on platelet aggregation by microfluidic chip Technology. METHODS: Microfluidic chip was used to simulate 80% fixed stenotic microchannel, and the hydrodynamic behavior of the stenotic microchannel model was analyzed by the finite element analysis module of sollidwork software. Microfluidic chip was used to analyze the adhesion and aggregation behavior of platelets in patients with different diseases, and flow cytometry was used to detect expression of the platelet activation marker CD62p. Aspirin, Tirofiban and protocatechuic acid were used to treat the blood, and the adhesion and aggregation of platelets were observed by fluorescence microscope. RESULTS: The gradient fluid shear rate produced by the stenosis model of microfluidic chip could induce platelet aggregation, and the degree of platelet adhesion and aggregation increased with the increase of shear rate within a certain range of shear rate. The effect of platelet aggregation in patients with arterial thrombotic diseases were significantly higher than normal group (P<0.05), and the effect of platelet aggregation in patients with myelodysplastic disease was lower than normal group (P<0.05). CONCLUSION The microfluidic chip analysis technology can accurately analyze and evaluate the platelet adhesion and aggregation effects of various thrombotic diseases unde the environment of the shear rate, and is helpful for auxiliary diagnosis of clinical thrombotic diseases.
Humans ; Microfluidics ; Platelet Adhesiveness ; Platelet Aggregation ; Blood Platelets/metabolism* ; Platelet Aggregation Inhibitors/pharmacology* ; Platelet Activation/physiology* ; Thrombosis

BACKGROUND: Platelet activation is an important process of atherothrombosis. However, there are few studies demonstrating the serial changes of platelet activation and the influences of the kinds of therapeutic strategies in atherosclerotic ischemic stroke from acute onset to the subacute phase. METHODS: We serially measured the expressions of CD63 on platelets in patients with atherosclerotic ischemic stroke (n=29) and compared them with normal subjects (n=52) and analyzed the effects to reduce the platelet activation according to the therapeutic strategies. RESULTS: The platelet CD63 expression (p<0.001) in atherosclerotic ischemic stroke significantly increased at 24 hours of ischemic stroke onset compared to normal subjects and their increment continually remained until 90 days after the ischemic events. Among the 29 patients, 12 patients treated with clopidogrel and aspirin showed significant decreases of the platelet CD63 expression (p=0.01) at 7 days compared to that at 24 hours. However, the reducing effect of this combination regimen disappeared after 90 days. CONCLUSIONS: These results suggest that the hyperexpression of CD63 on platelets in atherosclerotic ischemic stroke is not easily suppressed by regular anti-platelet medications.
Aspirin ; Blood Platelets* ; Brain Ischemia ; Humans ; Inflammation ; Platelet Activation* ; Stroke*

No abstract available.
Blood Platelets* ; Hypertension, Pulmonary* ; Lung Diseases, Obstructive* ; Platelet Activation*

In China, the evaluation of hemocompatibility of biomaterials is limited to hemolysis, coagulation time,and the number and morphology of platelets adhered on biomaterials. The present research, however, is aimed to establish a method for evaluating the function of sheet biomaterials in platelet activation. Platelet activation caused by glass, polyvinyl chloride or polymethylvinylsiloxane sheets was evaluated by measuring alpha-granule membrane protein (GMP-140) in platelet poor plasma, using a reasonable blood-material contact model vibrating at different speed. The result showed that the difference in platelet activation was not significantly different among the three above-mentioned materials at 140r/m or 200r/m. However, when it comes to 230r/m, significant difference was observed among these three groups, with glass > polyvinyl chloride > polymethylvinylsiloxane. But the order was reversed at 270r/m, which may be due to the different interfacial tension of different materials. Therefore, the method is suitable to evaluate platelet activation caused by sheet biomaterials, but an appropriate vibrating speed should be chosen. The interfacial tension plays an important role in the model and should be considered for results assessment.
Biocompatible Materials ; Humans ; Materials Testing ; methods ; Platelet Activation ; Surface Properties

In recent years a huge amount of clinical studies have proved that fluctuant hypertension could aggravate the damage of target organs and increase the incidence of acute cardio-/cerebrovascular events, when compared with stable hypertension [increased mean artery blood pressure (MBP)]. How to prevent and treat fluctuant hypertension and its damage of target organs has become one of the hot and difficult problems in the field of hypertension studies all over the world. Hypertensive patients often suffer from thromboembolic target organ damage. Platelet activation plays a key role in this progress, but its concrete mechanisms have not been clearly clarified. Based on the in-depth discussions on progress of fluctuant hypertension, its relationship with platelet activation and blood stasis syndrome of Chinese medicine (CM) in recent three years, we proposed, under the fluctuant hypertensive state, the prethrombotic state has occurred in the organisms, i.e., a pathological state featured by platelet activation, liable to have vulnerable thrombopoiesis, and accompanied by endothelial dysfunction. Blood stasis syndrome might occupy an important position in CM syndrome typing of fluctuant hypertension. Herbs for activating blood circulation and removing stasis might have an extensive application prospect.
Humans ; Hypertension ; blood ; prevention & control ; Integrative Medicine ; Platelet Activation