1.Whole Mitochondrial Genome Sequence of an Indian Plasmodium falciparum Field Isolate.
Suchi TYAGI ; Veena PANDE ; Aparup DAS
The Korean Journal of Parasitology 2014;52(1):99-103
Mitochondrial genome sequence of malaria parasites has served as a potential marker for inferring evolutionary history of the Plasmodium genus. In Plasmodium falciparum, the mitochondrial genome sequences from around the globe have provided important evolutionary understanding, but no Indian sequence has yet been utilized. We have sequenced the whole mitochondrial genome of a single P. falciparum field isolate from India using novel primers and compared with the 3D7 reference sequence and 1 previously reported Indian sequence. While the 2 Indian sequences were highly divergent from each other, the presently sequenced isolate was highly similar to the reference 3D7 strain.
DNA, Mitochondrial/*chemistry/*genetics
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Genetic Variation
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*Genome, Mitochondrial
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Humans
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India
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Malaria, Falciparum/parasitology
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Molecular Sequence Data
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Plasmodium falciparum/*genetics/isolation & purification
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
2.Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection.
Hyeong Woo LEE ; Sung Ung MOON ; Hye Sun RYU ; Yeon Joo KIM ; Shin Hyeong CHO ; Gyung Tae CHUNG ; Khin LIN ; Byoung Kuk NA ; Yoon KONG ; Kyung Suk CHUNG ; Tong Soo KIM
The Korean Journal of Parasitology 2006;44(1):49-54
In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
Recombinant Proteins/biosynthesis/genetics/*immunology
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Plasmodium vivax/isolation & purification
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Plasmodium falciparum/*immunology
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Molecular Sequence Data
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Malaria, Falciparum/blood/*diagnosis
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Humans
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Genes, Protozoan/genetics/immunology
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Fluorescent Antibody Technique, Direct/methods
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Escherichia coli/genetics
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Enzyme-Linked Immunosorbent Assay/methods
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Early Diagnosis
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DNA, Protozoan/chemistry
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DNA Primers/chemistry
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Cloning, Molecular/methods
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Base Sequence
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Antigens, Protozoan/biosynthesis/chemistry/genetics/*immunology
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Animals
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Amino Acid Sequence
3.A Locally Acquired Falciparum Malaria via Nosocomial Transmission in Korea.
Jung Yeon KIM ; Jeong Su KIM ; Mi Hyun PARK ; Young A KANG ; Jun Wook KWON ; Shin Hyeong CHO ; Byeong Chul LEE ; Tong Soo KIM ; Jong Koo LEE
The Korean Journal of Parasitology 2009;47(3):269-273
A 57-year old man who was admitted to an emergency room of a tertiary hospital with hemoptysis developed malarial fever 19 days later and then died from severe falciparum malaria 2 days later. He had not traveled outside of Korea for over 30 years. Through intensive interviews and epidemiological surveys, we found that a foreign patient with a recent history of travel to Africa was transferred to the same hospital with severe falciparum malaria. We confirmed through molecular genotyping of the MSP-1 gene that Plasmodium falciparum genotypes of the 2 patients were identical. It is suggested that a breach of standard infection control precautions resulted in this P. falciparum transmission between 2 patients in a hospital environment. This is the first report of a nosocomial transmission of falciparum malaria in Korea.
Africa
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Amino Acid Sequence
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Animals
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Cross Infection/parasitology/*transmission
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Fatal Outcome
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Humans
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Korea
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Malaria, Falciparum/parasitology/*transmission
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Male
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Merozoite Surface Protein 1/chemistry/genetics
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Middle Aged
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Molecular Sequence Data
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Plasmodium falciparum/chemistry/genetics/*isolation & purification
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Protozoan Proteins/chemistry/genetics
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Sequence Alignment
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Travel