1.Preparation and verification of antibodies for five rice receptor-like kinases.
Bo WANG ; Liyun LI ; Yinghao CAO ; Ziguang LIU ; Guozhen LIU
Chinese Journal of Biotechnology 2010;26(6):796-801
Receptor-like kinase involves self-incompatibility, male sterility, stress responses, and disease resistance. To better understand the physiological function and biological characteristics of rice receptor-like kinase, we cloned five predicted epitope fragments of rice receptor-like kinase. The purified fusion protein was used as antigen to immunize rabbit to get specific polyclonal antibodies. Western blotting analysis shows that the five receptor-like kinases were expressed in rice leaves.
Animals
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Antibodies, Monoclonal
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biosynthesis
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immunology
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Cloning, Molecular
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Epitopes
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immunology
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Immunization
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Oryza
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enzymology
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genetics
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Plant Proteins
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genetics
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immunology
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Plantibodies
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immunology
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metabolism
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Protein Kinases
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genetics
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immunology
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Rabbits