No abstract available.
Agglutination* ; Alisma* ; Animals ; Erythrocytes* ; Mice* ; Plantago*

Anticoccidial effects of the Plantago asiatica extract (PAE) were evaluated in chickens following oral infection with Eimeria (E.) tenella. This study was conducted on the 3-day-old chickens (n=30). Those animals were divided with 3 groups; PAE 0.1% treated/infected (n=10), PAE untreated/infected (n=10) and non-infected control (n=10). Chickens were fed a standard diet supplemented with or without PAE for 1 week prior to infection with E. tenella (10,000 sporulated oocysts per chicken). The effects of PAE on E. tenella infection were assessed by two parameters; fecal oocysts shedding and body weights gain. The PAE-fed chickens produced significantly reduced fecal oocysts (P<0.05) when compared to the E. tenella-infected group fed standard diet. Also, PAE-based diet, improved body weight loss caused by E. tenella infection. Our data demonstrated that PAE had remarkable anticoccidial activities against E. tenella. This finding might have implications for the development of anticoccidial drug. This study is the first to demonstrate anticoccidial effect of PAE on Eimeria parasites.
Animals ; Body Weight ; Chickens ; Diet ; Eimeria tenella* ; Eimeria* ; Oocysts ; Parasites ; Plantago*

Plantago major is not only used as medicinal herb but also an important model plant of ecology. Little work has been reported on the tissue culture of P. major. A reproducible system for direct shoot morphogenesis and callus induction of Plantago major L. 'Giant Turkish' was described. Using seed as explants, the adventitious buds were obtained 4 to 5 weeks following incubation on MS medium supplemented with 0.2 mg/L IAA and 1.0 mg/L TDZ. The frequency of adventitious buds was as high as 100%. The average number of buds per explant was 14.6. Random amplified polymorphic DNA analysis on 9 regenerants indicated that somaclonal variation occurred at DNA level. Using leaves as explants, calli were easily induced on MS medium supplemented with 1.0 mg/L NAA 3 weeks following inoculation. The frequency of callus induction can be as high as 98%. On MS medium containing 4.0 mg/L 6-BA, 25% of calli differentiated and the mean number of buds per piece of callus was 2.8. The buds developed roots on 11/2 MS medium and formed plantlets, 90% of which survived when transplanted to greenhouse.
Plantago ; growth & development ; Plants, Medicinal ; growth & development ; Tissue Culture Techniques ; methods

OBJECTIVETo study the chemical constituents of Lagotis brevituba.

METHODThe chemical constituents were isolated and purified by silica gel column chromatography, polyamide column chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of analysis of IR,NMR, 2D-NMR, and MS spectra.

RESULTTwo compounds were obtained and were identified as phenylethanoid glucosides, lagotiside A (1) and acteoside (2), respectively.

CONCLUSIONCompound 1 is a new compound and named as lagotiside A.

Glucosides ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Plant Extracts ; chemistry ; Plantago ; chemistry

OBJECTIVETo study on the chemical consitituents of Lagotis brevituba.

METHODThe chemical consitituents were isolated by silica gel column chromatography, polyamide column chromatography and semi-preparative HPLC, and their structures were identified by spectroscopic methods.

RESULTEight compounds were isolated and they were identified as beta-sitosterol (1), succinic acid (2), luteolin-7-O-beta-D-glucoside (3), uracil (4), apigenin (5), chrysoeriol (6), chrysoeriol-7-O-beta-D-glucoside (7), and apigenin-7-O-beta-D-glucoside (8).

CONCLUSIONCompound 4-8 were isolated from L. brevituba for the first time, and among them, compound 7 and 8 were isolated from genus Lagotis for the first time.

Chromatography, High Pressure Liquid ; Organic Chemicals ; analysis ; isolation & purification ; Plantago ; chemistry

This study was conducted to investigate the effects of foliar endophytic fungi and arbuscular mycorrhizal fungi (AMF) on plant community structure in experimental microcosms containing an assemblage of five species of plants (Oenothera odorata, Plantago asiatica, Trifolium repens, Isodon japonicas and Aster yomena). Leaves of Sasa borealis, Potentilla fragarioides, and Viola mandshurica were collected in Chungbuk, Korea. Endophytic fungi were isolated from the surface sterilized leaves and identified to species level using molecular and morphological techniques. Four isolates of the endophytic fungi were inoculated to the leaves of host plants in the microcosms. Also, three species of AMF spores were extracted from pure cultures and the mixture of the three species inoculated to the roots of the plants. After four months of growth in a green house, effects of both symbiotic fungi on plant species diversity, community composition and productivity were examined. The plant species diversity showed significant differences with inoculation of the symbiotic fungi. Results indicate that AMF significantly affect plant productivity and plant community structure.
Chungcheongbuk-do ; Efficiency ; Endophytes ; Fungi* ; Isodon ; Korea ; Mycorrhizae ; Plantago ; Plants* ; Potentilla ; Sasa ; Spores ; Trifolium ; Viola

OBJECTIVETo explore the affection factors to expansion degree of seeds of Plantaginis caused by different processing methods.

METHODDetermination of expansion degree and analysis by gel chromatography.

RESULTThe values of expansion degree were decreased and the structures of polysaccharides were changed for the processed products compared with their crude seeds.

CONCLUSIONThe polysaccharies in seeds of Plantaginis were degraded after toasted without additive and roasted with salt, which further affected the expansion degree.

Hot Temperature ; Plantago ; chemistry ; classification ; Plants, Medicinal ; chemistry ; Polysaccharides ; analysis ; Seeds ; chemistry ; Species Specificity ; Technology, Pharmaceutical ; methods

OBJECTIVETo study the high-yield and high-quality cultivating technology of Plantago asiatica, adapt to the demands of GAP.

METHODThe main factors which influenced the yield and quality in the production process of P. asiatica was studied.

RESULTThe standard system cultivating technology of high yield and quality of P. asiatica was established.

CONCLUSIONThe seeds of P. asiatica can reach the objective of high yield and high quality on the basis of this cultivating technology.

China ; Fertilizers ; Pest Control ; Plant Diseases ; Plantago ; growth & development ; Plants, Medicinal ; growth & development ; Quality Control ; Seeds ; Soil

BACKGROUND AND OBJECTIVE: The Allergodip(R) multi-allergen screening test is a simple method for measuring serum-specific IgE to common inhalant allergens simultaneously. The dipstick contains nine separate allergen pads including D. pteronyssinus, Alternaria, cat epithelium, dog epithelium, English plantain, mugwort, birch, rye and six-grass mixture pollen extracts, as well as positive and negative controls. This study was performed in order to evaluate sensitivity and specificity of this test in comparison to skin prick test. MATERIALS AND METHOD: We compared the results obtained by skin prick test (PT) to those obtained by Allergodip(R) test (AT) in 30 patients with various allergic diseases. The skin reactivity was presented as allergen to histamine ratio (A/H) and results of the AT test were assessed visually and classified into Allergodip(R) class 0-4. RESULTS: Compared with PT, results of AT gave a sensitivity of 55.3% and a specificity of 93.8% when the A/H > OR = 3 was considered a positive response for PT. The agreement rate between positive PT and positive AT to each antigen was 82.6% in D. pteronyssinus, 82.9% in Alternaria, 71.1% in dog epithelium, 57.2% in mugwort pollen, 57.2% in birch pollen, 49.9% in grasses mixture pollen, 23.6% in cat epithelium and 6.7% in rye grass pollen, respectively. Significant correlations were noted between PT and AT classes in D. pteronyssinus, Alternaria, dog epithelium, mugwort pollen, birch pollen, and in grasses mixture pollen (p < 0.05, respectively), but not in cat epithelium or rye grass pollen. CONCLUSION: We concluded that Allergodip(R) test is useful in screening serum-specific IgE antibodies against multiple allergens at one time.
Allergens ; Alternaria ; Animals ; Antibodies ; Artemisia ; Betula ; Cats ; Dogs ; Epithelium ; Histamine ; Humans ; Hypersensitivity* ; Immunoglobulin E ; Lolium ; Mass Screening* ; Plantago ; Poaceae ; Pollen ; Secale ; Sensitivity and Specificity ; Skin

In a large number of a1lergic cases, inhalant allergens, particularly airborne pollens and mo1ds am important causative, triggering agents in respiratory, even in skinanergy. It is essential for diagnosis and treatment of airborne allergy to survey the airborne pollen and mo1d in the patient's living area. Rotorod samp1er was installed on the well-ventilated rooftop of Hanyang Kuri Hospital in Kuri-shi, Kyunggi-do as the vicinity of Seoul. Airborne allergens were collected from October 1, 1995 to September 30, 1996 daily and were stained with Calberla`s fuchsin and were identified, counted under the light microscope (400Xfor pollen, 1000Xfor mold). The data were compared with the local daily weather report. The airborne pollen was found from 12th February to 22nd December. The maximal period for pollen collection was 7th-25th May (Maximal count:492 grains/ August. There were Alnus, Betula, Pinus, Quercus, Acer, Ulmus, PopuluS, Cupressaceae, Ginkgo, Corylus, Salk in the tree season from middle of February to middle of July. The grass season ranged from middle of March to end of November. There were Artemisia, Ambrosia, HumuluS, Chenopodium-Amaranthus, rumex, Plantago, Trifoium, Humulus, Chrysanthemum, Cyperus in the weed season ranged from middle of July to end of December. Airborne molds were collected all through the year except JanuaIgamma but, counted maximally from the middle of June to end l August. There were CladosPorium, Alternaria, ascospore of Leptospheria, Periconia, Myxomycete, Drechs1era, Rust urediniospores, Basidiospore, ToruIa, Xylariaceae, IOidimn, Nigrospom, Stempi1y1ium, Amphispheda, Fusarium, Aspergil1us. In Korea, there are great number of the allergic patients, but we have had a little records and data about study of relationship between the allergic diseases and airborne environmental allergens. we will reported those results completely in future.
Acer ; Allergens ; Alnus ; Alternaria ; Ambrosia ; Artemisia ; Betula ; Chrysanthemum ; Cladosporium ; Corylus ; Cupressaceae ; Cyperus ; Diagnosis ; Fungi* ; Fusarium ; Ginkgo biloba ; Gyeonggi-do* ; Humans ; Humulus ; Hypersensitivity ; Korea ; Myxomycetes ; Pinus ; Plantago ; Poaceae ; Pollen ; Populus ; Quercus ; Rosaniline Dyes ; Rumex ; Seasons ; Seoul ; Trees ; Ulmus ; Weather