OBJECTIVETo study the chemical constituents in the roots of Indigofera pseudotinctoria.

METHODThe constituents were isolated by column chromatography and their structures were elucidated by spectroscopic methods.

RESULTFourteen compounds were isolated from the ethanol extract of the plant and identified as maackiain (1), 3beta-hydroxy-olean-9 (11), 12-diene (2), 12-oleanen-3,11-dione (3), 3beta-acetoxy-12-oleanen-11 -one (4), formononetin (5), formononetin-7-O-beta-D- glucoside (6), 7,4'-dihydroxy-3'-methoxy isoflavone (7), afromosin (8), genistein (9), calycosin-7-O-beta-D-glucoside (10), vicenin-2 (11), isoliquiritigenin (12), beta-sitosterol (13), and daucosterol (14).

CONCLUSIONAll compounds were isolated from the plant for the first time, eleven compounds (except 1, 13 and 14) were isolated from the genus Indigofera for the first time.

OBJECTIVETo investigate the nonvolatile chemical constituents from the ethanol extract of the stems of Pogostemon cablin.

METHODThe constituents were isolated and purified by repeated column chromatography on silica gel and Sephadex LH-20. Their structures were identified by physicochemical properties and spectroscopic analysis.

RESULTTwelve compounds were isolated and identified as tilianin (1), diosmetin-7-O-beta-D-glucopyranoside (2), 3"-O-methylcrenatoside (3), uracil (4), soya-cerebroside I and II (5), agastachoside (6), apigenin-7-O-(3", 6"-di-(E) -p-coumaroyl) -beta-D-galactopyranoside (7), 5-hydroxy-3, 3', 4', 7- tetramethoxy flavone (8), 4', 5-dihydroxy-3, 3', 7-trimethoxyflavone (9), acacetin (10), crenatoside (11), isocrenatoside (12).

CONCLUSIONCompounds 1, 2, 4-7, 10 were isolated from the genus Pogostemon for the first time.

OBJECTIVETo study the chemical constituents of Viola tianshanica.

METHODCompounds were isolated by silica column, pharmadex LH-20 column and polyamide column, and their structures were elucidated by UV, IR, ESI-MS and NMR.

RESULTSix compounds were isolated and identified as daucosterol (1), kaempferol-7-O-beta-D-glucopyranoside (2), kaempferol- 3-O-beta-D-glucopyranoside (3), isorhamnetin-3-O-beta-glucoside (4), kaempferol (5) and quercetin (6).

CONCLUSIONCompounds 2-5 were isolated from this plant for the first time.

OBJECTIVETo study the chemical constituents contained in ethanol extracts from aerial parts of Emilia sonchifolia.

METHODThe compounds were separated and purified with various chromatographic techniques, and their structures were identified on the basis of physicochemical properties and spectral data.

RESULTFifteen compounds were separated from ethyl acetate fraction of 90% ethanolic extract and identified as rhamnetin (1), isorhamnetin (2), quercetin (3), luteolin (4), tricin-7-O-beta-D-glucopyranoside (5), 8-(2"-pyrrolidinone-5"-yl) -quercetin (6), 5, -2', 6'-trihydroxy-7, 8-dimethoxyflavone-2'-O-beta-D-glucopyranoside (7), succinic acid (8), fumaric acid (9), p-hydroxybenzoic acid (10), 4-hydroxy isophthalic acid (11), 3, 4-dihydroxycinnamic acid (12), esculetin (13), isowedelolactone (14) and uracil (15), respectively.

CONCLUSIONAll compounds except compound 3 were separated from this genus for the first time.

OBJECTIVETo reveal the mutative discipline of the contents of the phenols in samples from different provenances.

METHODThe contents of magnolol and honokiol in samples from the filial generation of Guanxi, Hubei and Zhejiang provinces were determined respectively by HPLC.

RESULTThere were significant variations between provenances, the contents of honokiol, magnolol and phenols were the highest in samples from Hubei province, those were the lowest in samples from Zhejiang province. There were not only significant differences in polyphenol content between provenances, but also in families in the same provenance and individuals in families.

CONCLUSIONMagnolia polyphenol content and appearance traits were significantly related. The type with the small convex tip (original form) were superior to other types of M. officinalis judging from the characteristics of tree classification. The high quality of M. officinalis should actually be attributed to their local varieties and the breeding strategy of M. officinalis should be paid full attention to the good provenances, good families and good individuals.

OBJECTIVETo investigate the chemical constituents of Peperomia tetraphylla.

METHODThe constituents of EtOAc-soluble portion were isolated and purified by chromatography. Their structures were identified by spectral features.

RESULTSix amides were isolated and identified as, aristololactam All (1), aristololactam B II (2), N-trans-feruloyltyramine (3), N-trans-sinapoyltyramine (4), N-trans-feruloylmethoxytyramine (5), N-p-coumaroyltyramine (6).

CONCLUSIONAll compounds were isolated from this plant for the first time.

The chemical constituents of Parthenocissus quinque were investigated. The chemical constituents were isolated by column chromatography on silical gel and sephadex LH-20. Their structures were elucidated on the basis of spectral analysis and of comparison of physical constant. Nine compounds were isolated from this plant and the structures of them were identified as 3,4,5-trihydroxy- benzoic acid (1), piceatannol (2), resveratrol (3), resveratrol trans-dehydrodimer (4), cyphoste mmin B (5), pallidol (6), cyphostemmin A (7), quercetin-3-O-alpha-L-rhamnoside (8), myricetin-3-O-alpha-L-rhamnoside (9), respectively. Compounds 1, 4-9 were isolated from this plant for the first time.
Plant Extracts ; analysis ; isolation & purification ; Vitaceae ; chemistry

OBJECTIVETo provide evidences for the pharmacognostical and chemical identification and the further development of Lysinotus wilsonii.

METHODThe paraffin section method was used for the microscopic identifications of stems and leaves. The slide with chloral hydrate was applied for the microscopic identifications of the powder of stems and leaves. The HPLC was used for the identification of the phenylpropanoids.

RESULTAn obvious periderm consisted of a line of inseparable cells, lots of stone cells existed in the periderm and cortex, narrow ring of xylem and broad pith could be easily observed in the stem transaction of L. wilsonii. Epidermis was composed of one lines of parenchyma, three lines of epithelial cells in the side of above epidermis, palisade tissue was composed of 2-3 lines of square and thin cells and amphicribral vascular bundle in transaction of midrib also could be observed in the leaf transaction of L. wilsonii. Long xylem fibers, lots of pitted vessels, stone cells and anomocytic type stomas existed in the powder of L. wilsonii. Acteosidel and caleolarioside B were detected in L wilsonii.

CONCLUSIONThe pharmacognostic and chemical characteristics of L. wilsonii can be used for authentication of the plant.

OBJECTIVETo study the chemical constituents in the rhizoma of Iris dichotoma.

METHODThe chemical constituents were isolated by various column chromatographic methods. The structures of the compounds were elucidated on the basis of physiochemical properties and spectral analysis.

RESULTEleven compounds, hispidulin (1), rhamnocitrin (2), iristectorigenin A (3), 4', 5, 7, 8-tetrahydroxy-6-methoxy isoflavone (4), 6-hydroxybiochanin A (5), iristectorin B (6), iristectorigenin A (7), kaempferol-7-methyl ether (8), tamarixetin-7-glucoside (9), iristectorin A (10), 3', 3, 5-trihydroxy-4', 7-dimethoxy-flavone-3-O-beta-D-galactopyranoside (11) were isolated and identified.

CONCLUSIONCompounds 1-11 were obtained from this plant for the first time.

OBJECTIVETo study the chemical constituents of Swertia macrosperma.

METHODThe air-dried whole plants of Swertia macrosperma were extracted with boiling water. The extract was concentrated to a small amount of volume and extracted with petroleum ether, EtOAc and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the EtOAc fraction, and identified based on spectral analyses (MS, 1H-NMR, 13C-NMR).

RESULTThirteen compounds were isolated from S. macrosperma, and were characterized as norbellidifolin (1), 1-hydroxy-3,7, 8-trimethoxy-xanthone (2), norswertianolin (3), swertianolin (4), 1,3,7,8-tetrahydroxyxanthone-8-O-beta-D-glucopyranoside (5), swertiamatin (6), decentapicrin (7), coniferl aldehyde (8), sinapaldehyde (9), balanophonin (10), together with beta-sitosterol, daucosterol, and oleanolic acid .

CONCLUSIONCompounds 2, 4-10 were obtained from Swertia macrosperma for the first time.