1.Results of the study on the anti- Helicobacter pylori in vitro of active components from leaves of peper beetle
Journal of Practical Medicine 2002;435(11):22-25
Helicobacter pylori’s role in pathogenesis of gastroduodenitis and gastroduodenal ulcer has been documented in recent studies. Treatment efficacy has been changed because H.pylori get more and more persistent to treatment. Using antibiotic from herbal is the aim of our study. A product extracted from Piper beetle. L, a strongly antibiotic agent in vitro, can eradicate HP? The specimen was taken from gastric mucosa of patients with gastritis. These specimens have been crushed and cultured in brain- heart agar medium in the atmosphere of 10% CO2, and to: 37oC. If the bacteria developed, we would measure their sensitivity to total extract (Betelvine) from Piper beetle. L. 35 strains of HP developed from 56 specimens were assessed their sensitivity by using round paper pieces, which had been soaked with or Betelvine, either Tetracycline, Ampicilline, metronidazole- 100% strains of HP were sensitive to Betelvine with antibacterial rings from 12mm to 40mm.- 100% strains of HP were not sensitive to metronidazole, all antibacterial rings of metronidazole were O mm. 3,32% strains of HP were not sensitive to Tetracycline. - 12,9% trains of HP weren’t sensitive to Ampiciline.
Piper
;
Helicobacter
;
therapeutics
2.Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
Wan Joo Tan ; Tu Anh Vu Thanh ; Elissa Stella Rafael ; Yi Shang Chen ; Freddy Kuok San Yeo
Malaysian Journal of Microbiology 2021;17(2):165-177
Aims:
Piper nigrum L. (black pepper) is an economically important commodity plant in Malaysia, which generated RM
200.95 million from pepper export in the year of 2018. However, the increase in pepper production is restricted by
diseases. Fusarium wilt is one of the major diseases of P. nigrum L. The objectives for this study were to isolate
Fusarium spp. associated with Fusarium wilt of P. nigrum L. from selected pepper farms in the northwestern region of
Sarawak and to characterize the Fusarium spp. isolated morphologically and molecularly.
Methodology and results:
Fusarium spp. were isolated from diseased root samples. The pathogen was grown on
potato dextrose agar (PDA) under dark condition at circa (ca.) 25 °C for morphological characterisation. Molecular
characterisation was done by using internal transcribed spacer (ITS). Phylogenetic tree was constructed to study the
genetic relationship of the isolates. Fusarium solani, F. oxysporum, F. proliferatum were the three Fusarium species
identified. There were variations in morphological characters observed between and among the species, including the
colony form, margin, elevation, surface appearance and pigmentation. No distinctive morphological characteristic was
specific to a location. In addition, growth rate, macroconidia sporulation rate, and microconidia sporulation rate of the
isolates were not correlated. In molecular phylogeny, the three Fusarium species were separated into three distinct
clades representing the three identified species. The genetic relatedness between isolates within each species was
depicted in the tree.
Conclusion, significance and impact of study
Variations were observed among isolates in this study based on
morphological and molecular characterization. This study would contribute information on the variations of Fusarium
spp. associated with Fusarium wilt of P. nigrum L. from the northwestern region of Sarawak.
Fusarium
;
Fusariosis
;
Piper nigrum
3.Antibacterial activity of Sireh (Piper betle L.) leaf extracts for controlling bacterial leaf blight diseases in rice plant
Nor Umaira Abu Asan ; Yaya Rukayadi ; Geok Hun Tan
Malaysian Journal of Microbiology 2022;18(3):291-300
Aims:
This study aimed to determine the antibacterial activity of Piper betle L. leaf extract against Xanthomonas oryzae pv. oryzae that causes bacterial leaf blight in rice plant.
Methodology and results:
The antibacterial activity of the P. betle leaf extract (100, 50, 25 and 12.25 mg/mL) with four different solvents (methanol, ethyl acetate, hexane and acetone) was evaluated using a disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. The inhibition zone of methanolic extract appeared to have the maximum diameter compared to those of other extracts, which is 32.67 mm at a concentration of 100 mg/mL, followed by 30.33 mm, 22.00 mm and 20.30 mm for the concentrations of 50 mg/mL, 25 mg/mL and 12.5 mg/mL, respectively. The MIC and MBC values of the methanolic extract were 0.625 mg/mL suggesting that the extract has a bactericidal effect on X. oryzae pv. oryzae (Xoo). The time-kill curve studies revealed that the 1× MIC (0.625 mg/mL) concentration of methanolic extract had a time and concentration-dependent killing effect on Xoo. Gas chromatography-mass spectrometry (GC-MS) analysis of methanol extract revealed the presence of eugenol acetate (29.53%), 4-allyl-1,2-diacetoxybenzene (29.51%) and 2,3-dimethyl benzoic acid (22.82%) as major compounds.
Conclusion, significance and impact of study
The methanolic leaf extract of P. betle was proven to have an effective inhibitory effect on Xoo and may have the potential to be used as an alternative management strategy for controlling rice diseases. In the future study, the methanolic leaf extract of P. betle is one of the recommendations to be applied in glasshouse and field trials.
Piper betle
;
Oryza--microbiology
4.Physical Activity and Fatigue in Patients with Cancer.
Journal of Korean Oncology Nursing 2010;10(1):30-37
PURPOSE: The purpose of this study was to identify the relationship between physical activity and fatigue in patients with cancer. METHODS: The participants of the study were 118 patients who had been diagnosed with cancer at least more than three months before at a university hospital. Physical activity was measured by IPAQ Korean version (2006), and fatigue by the Revised Piper Fatigue Scale. The collected data were analyzed using frequency, percentage, t-test, ANOVA, Pearson correlation coefficient. RESULTS: Continuous physical activity overall in this study was on average 2,154.03+/-2,845.86 MET, and the overall degree of fatigue of the participants was 4.47+/-1.80. There were significant negative correlations between fatigue and moderate physical activity. CONCLUSION: The findings of the study suggested that moderate physical activity is effective for improving fatigue in cancer patients.
Fatigue
;
Humans
;
Motor Activity
;
Piper
5.Relationship between Fatigue and Sleep Quality in Patients with Cancer.
Journal of Korean Academy of Adult Nursing 2002;14(3):378-389
PURPOSE: The purpose of this study was to identify the relationship of fatigue and quality of sleep in patients with cancer. METHOD: The data was collected from January to February 2001. Study objects were recruited K university hospital in Busan, Korea. Their fatigue was measured using the Revised Piper Fatigue Scale developed by Piper et. al(1998), and quality of sleep was measured using Quality of Sleep Questionare by Oh et. al(1998). RESULT: 1) The fatigue score was mean 114.80+/-34.88(range: 22-220). The sub dimension that showed behavior/severity score at 33.70+/-13.89, affective score at 24.23+/-3.33, sensory score at 27.74+/-12.51, and cognitive/mood score at 29.11+/-3.71. And sleep quality score was mean 37.32+/-8.18. 2) There was a significant difference in religion(F=4.157, P= .008), present therapy(F=2.536, P= .043), past therapy(F= 6.625, P= .000), major caregiver(F=3.133, P= .028), and change of weight(F=7.965, P= .006), according to general characteristics in the fatigue in patients with cancer. 3) There was a significant difference in present pain(t=-2.103, P= .037) and change of weight(F=5.484, P= .005), according to general characteristics in the sleep quality in patients with cancer. 4) There was a significant negative correlation between fatigue and quality of sleep(r=- .340, P= .000). CONCLUSION: Patients with cancer experience in fatigue. Increase in fatigue are associated with decreases in quality of sleep. Nurses must provide patients with nursing care about the occurrence of fatigue and interventions to deal with sleep disturbance.
Busan
;
Fatigue*
;
Humans
;
Korea
;
Nursing Care
;
Piper
7.In Vitro Effect of Fungicides, Plant Extracts and Smoke on Conidial Germination of Fusarium oxysporum Root Rot Pathogen of Piper betle.
Shahidul ALAM ; M Rafiqul ISLAM ; Montaz Ali SARKAR ; Arfatun Nahar CHOWDHURY ; M S ALAM ; Min Woong LEE
Mycobiology 2004;32(1):42-46
Five fungicides such as rovral, bavistin, cupravit, dithane M-45 and thiovit were tested against conidial germination of Fusarium oxysporum. Dithane M-45 was the most effective against the fungus. Rests of the fungicides were more or less effective in the inhibition of conidial germination after 5~30 minutes immersion in 500~2500 ppm concentration. Five plant extracts(leaf extracts of Ocimum sanctum, Lantana camera, Calotropis procera, Azadirachta indica and Vinca rosea) found to be more or less effective against the fungus. 'Dhup' somke have good inhibitory effect on conidial germination of F. oxysporum.
Azadirachta
;
Calotropis
;
Fungi
;
Fusarium*
;
Germination*
;
Immersion
;
Lantana
;
Ocimum
;
Piper betle*
;
Piper*
;
Plant Extracts*
;
Plants*
;
Smoke*
;
Vinca
8.Chemical constituents from air-dried Piper longum.
Wenfeng LIU ; Zhiyong JIANG ; Jijun CHEN ; Xuemei ZHANG ; Yunbao MA
China Journal of Chinese Materia Medica 2009;34(9):1101-1103
OBJECTIVETo study the chemical constituents of Piper longum.
METHODThe whole plant of air-dried P. longum. was extracted with 95% EtOH. The EtOH extract was suspended in H2O and extracted with petroleum ether, CHC13 and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the CHCl3 fraction, and identified based on spectral analyses (MS,1H-NMR, 13C-NMR).
RESULTThreeteen compounds were isolated from P. longum, and were characterized as 1-(3',4'-methylenedioxyphenyl)-1E-tetradecene (1), 3-(3', 4'-methylenedioxophenyl)-propenal (2), piperoic acid (3), 3',4'-di-hydroxy-biabola-1,10-diene (4), eudesm-4(15)-ene-1beta, 6alpha-diol (5), 7-epi- eudesm-4( 15)-ene-1beta, 6beta-diol (6), guineesine (7), piperine (8), pipericide (9), 2E, 4E-dienamide (10), (2E, 4E, 8E) -N-isobutylhenicosa-2,4,8-trienamide (11), piperlonguminine (12), methyl piperate (13),
CONCLUSIONCompounds 1-6 were obtained from P. longum for the first time.
Air ; Desiccation ; Organic Chemicals ; analysis ; isolation & purification ; Piper ; chemistry
9.Chemical constituents from Piper longum.
Wenfeng LIU ; Zhiyong JIANG ; Jijun CHEN ; Xuemei ZHANG ; Yunbao MA
China Journal of Chinese Materia Medica 2009;34(22):2891-2894
OBJECTIVETo study the chemical constituents of Piper longum.
METHODThe whole plant of air-dried P. longum was extracted with 95% EtOH. The EtOH extract was suspended in H2O and extracted with petroleum ether, CHCl3 and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the CHCl3 fraction, and identified based on spectral analyses (MS, 1H-NMR, 13C-NMR).
RESULTEleven compounds were isolated from P. longum, and were characterized as coumaperine (1), N-5-(4-hydroxy-3-methoxyphenyl)-2E-pentenoyl piperidine (2), piperolactam A (3), 1-[1-oxo-5 (3,4-methylenedioxyphenyl) -2E,4E-pentadienyl] -pirrolidine (4), 1-[1-oxo-5 (3,4-methylenedioxyphenyl) -2E-pentenyl] -pirrolidine (5), 1-[1-oxo-9 (3,4-methylene dioxyphenyl)-2E, 8E-nonadienyl] -pyrrolidine (6), (R)-(-) -turmerone (7), octahydro-4-hydroy-3alpha-methyl-7-methylene-alpha-(1-methylethyl)-1H-indene-1-methanol (8), (+) -aphanamol I (9), bisdemethoxycurcumin (10), demethoxycurcumin (11).
CONCLUSIONCompounds 1-11 were obtained from P. longum for the first time.
Drugs, Chinese Herbal ; chemistry ; Magnetic Resonance Spectroscopy ; Piper ; chemistry
10.Effects of aqueous and pelletized admixute of Piper nigrum L on the oviposition behavior of Aedes aegypti mosquitoes and its larvicidal-ovicidal activity.
Briones Annabelle V ; Garbo Alicia G ; Casa Edmar P ; Bion Hermelina H ; Almanzor Nuna E ; Bernardo Severino T
Acta Medica Philippina 2012;46(3):55-58
OBJECTIVE: The aqueous and pelletized admixture of Piper nigrum L. was evaluated for its oviposition response and larvicidal activity against Aedes aegypti mosquitoes.
METHODS: The aqueous and pelletized extract of Piper nigrum L. was prepared and first tested in the laboratory. Efficiency is evaluated using the mosquito-chamber test. A small-scale field test was also done to determine the oviposition response of the pepper extract to ovicidal-larvicidal (OL) traps. Larvicidal bioassay following the WHO standard protocols with slight modification at different concentrations was performed.
RESULTS: Results of the mosquito chamber test in the laboratory showed that the aqueous solution exhibited an increasing rate of oviposition attraction of female Aedes aegypti to increasing rate of concentration with an average of 70% attraction at 1000 ppm as compared to 30% attraction to OL traps with water alone. The aqueous pepper-based solution and pelletized pepper solution at 1000 and 2000 ppm are considered attractants to Aedes mosquitoes. Both solutions have oviposition activity index (OAI) of > +0.3. It was also field tested on the 10 buildings within the Department of Science and Technology (DOST) compound. Results showed an oviposition ratio two times better in both the 1000 ppm concentration of the aqueous pepper-based solution and pelletized pepper solution as compared to the control. The positive ovitrap index was in the range of 78%-84% for both the aqueous pepper-based and pelletized pepper against water which is 70.0%. Larvicidal activity of the aqueous pepper-based solution against 3rd larval instars of Aedes aegypti at increasing dosages from 75mg/1 to 600 mg/I had an LCso of 127 mg/I and 395 mg/I for LC90 The LCso for the solution with pelletized pepper at the same dosing concentration is 117 mg/I with LC90 of 285 mg/1. The results also showed that these can be used to control larval instars of the Aedes aegypti mosquitoes.
CONCLUSION: The overall results indicate that the aqueous and pelletized extracts of Piper nigrum L. are effective in attracting the Aedes aegypti mosquitoes for oviposition and exhibit a larvicidal activity.
Animal ; Aedes ; Piper Nigrum ; Larva ; Oviposition ; Water ; Biological Assay