Aim To discuss the impact of important ac-tive ingredient of garlic diallyl sulfide———DATS on platelet activating factor (PAF ) mediated melanoma metastasis and its mechanisms.Methods ①MTT was used to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell growth number;②Scratch test and transwell were employed to test the effect of different concentrations of DATS on B16F10 and A375 melanoma cell migration;③ West-ern blot was used to test the effect of DATS on expres-sion of MMP-2,ERK,p38 induced by PFA;④Intrave-nous injection of tumor metastasis model was used to check the inhibition of DATS in PAF-mediated melano-ma metastasis.Results B16F10 cells relative growth rate fell to 73.21% and 48.78%,respectively,when DATS concentration reached 50 and 100 μmol·L-1 . DATS inhibited the levels of PAF-induced migration of melanoma cells B16F10 and vertical migration signifi-cantly,and inhibited B16F10 cells migration induced by PAF through inhibiting the expression of MMP-2, paxillin protein,FAK and other proteins.Conclusion DATS can significantly inhibit PAF-induced tumor metastasis, which is related to the inactivation of MAPKs.

Tumor metastasis is one of the most important biologi-cal characteristics of malignant tumor, and it is also the main factor resulting in poor prognosis and leading to failure of treat-ment. In recent years, studies have shown that the extracellular matrix ( ECM) of tumor microenvironment plays a critical role in tumor metastasis. Tumor ECM fibrogenesis could form the cross-linked network structure, which not only provides nutrition and support to tumor, also it is necessary to tumor growth and inva-sion. These research results indicate that blocking ECM fibro-genesis may exert an inhibitory effect on tumor metastasis. Therefore, targeting ECM fibrogenesis has become a particularly attractive strategy as it can be used in the treatment of metasta-sis-related diseases. The ECM fibrogenesis in tumor is reviewed in this paper as well as the treatment strategies on tumor metas-tasis by targeting ECM fibrogenesis, which may provide refer-ences for follow-up research and clinical treatment.

Objective To investigate the effect of perceived service quality improvement during reasonable use of intraoperative period among patient′s relatives by the application of process management model in Ophthalmology Department.Methods We investigated 100 relatives of patients who had surgery from July to September in 2013 by questionnaire.The investigation included the appropriate time to educate health knowledge and satisfaction degrees.Results The relatives′satisfaction degree was beyond 95% at one-to-one tutorials, repeated surgical knowledge on TV, the process of changing and attitude of medical staff in waiting time.For the appropriate time to educate, 70%of relatives believed the waiting time was the right time, but 27%of them preferred acquired after operation, and 3%of them had indifference of time.Conclusions Process management model plays a significant role to improve the perceived service quality of the relatives during the waiting time when the patients are having surgeries in Ophthalmology Department.

Objective:To examine the association between working hours and urinary sodium and potassium excretion levels in a population undergoing health examinations and the potential mediating effect of timely eating behaviors.Methods:In this cross-sectional study, 64 400 people who received health checkups from August 2017 to August 2022 at the Third Xiangya Hospital of Central South University were recruited as study subjects. General information on socio-demographic characteristics of the enrolled subjects were collected using the health checkup self-assessment questionnaire in the Expert Consensus on Basic Items for Health Checkups. Urinary excretion levels of sodium and potassium were measured with the Kawasaki method. The association between working hours and urinary sodium and potassium excretion levels was checked by using Pearson′s correlation analysis. The relationships between timely eating behaviors, working hours, and urinary sodium and potassium excretion levels and their ratios were analyzed using logistic regression and linear regression models, while the mediating mechanisms involved was also examined.Results:Among the 64 400 subjects, there were 39 274 males (60.98%) and 25 126 females (39.02%), the mean age was (43.80±11.13) years. There were 16 980 individuals (26.37%) with an average working time exceeding 8 hours per day. Additionally, 4 332 subjects (6.73%) were unable to eat three meals on time. The mean urinary sodium and potassium excretion and their ratios were (4.12±1.86) g/d, (2.06±0.80) g/d, 2.05±0.66, respectively. There was a significant positive correlation between working hours and urinary sodium excretion, urinary potassium excretion, and their ratios ( r=0.034, 0.021, 0.032, respectivley); it showed that timely eating behavior had a significant negative correlation with urinary sodium and potassium excretion ( r=-0.022, -0.019, respectivley) (all P<0.001). There was a partial mediating effect of timely eating behavior in the association between working hours and urinary sodium excretion (effect value of -0.006), and after stratifying the labor intensity, this mediating effect was only found among individuals engaged in light physical labor (both P<0.001). Conclusions:Prolonged working hours leads to increased levels of urinary sodium and urinary potassium excretion, and timely eating behavior facilitates salt/sodium reduction in light-duty workers.

Background::Pancreatic adenocarcinoma (PAAD) is an extremely lethal malignancy. Identification of the functional genes and genetic variants related to PAAD prognosis is important and challenging. Previously identified prognostic genes from several expression profile analyses were inconsistent. The regulatory genetic variants that affect PAAD prognosis were largely unknown.Methods::Firstly, a meta-analysis was performed with seven published datasets to systematically explore the candidate prognostic genes for PAAD. Next, to identify the regulatory variants for those candidate genes, expression quantitative trait loci analysis was implemented with PAAD data resources from The Cancer Genome Atlas. Then, a two-stage association study in a total of 893 PAAD patients was conducted to interrogate the regulatory variants and find the prognostic locus. Finally, a series of biochemical experiments and phenotype assays were carried out to demonstrate the biological function of variation and genes in PAAD progression process.Results::A total of 128 genes were identified associated with the PAAD prognosis in the meta-analysis. Fourteen regulatory loci in 12 of the 128 genes were discovered, among which, only rs4887783, the functional variant in the promoter of Ring Finger and WD Repeat Domain 3 ( RFWD3), presented significant association with PAAD prognosis in both stages of the population study. Dual-luciferase reporter and electrophoretic mobility shift assays demonstrated that rs4887783-G allele, which predicts the worse prognosis, enhanced the binding of transcript factor REST, thus elevating RFWD3 expression. Further phenotypic assays revealed that excess expression of RFWD3 promoted tumor cell migration without affecting their proliferation rate. RFWD3 was highly expressed in PAAD and might orchestrate the genes in the DNA repair process. Conclusions::RFWD3 and its regulatory variant are novel genetic factors for PAAD prognosis.