p38 mitogen-activated protein kinase (MAPK) is an important intracellular signaling transduction pathway.Its major role is involved in inflammatory regulation and apoptosis.p38 MAPK is activated during the cerebral ischemia.Its expression level and the upstream and downstream protein phosphorylation levels will change.Ischemic preconditioning may mediate the survival or death of neuronal cells after cerebral ischemia through signaling transduction pathway.

Based on the characteristics of the onset age of acne,relationship of both inter-promotion and inter-restraint among the five elements between the liver and the lung,route of main and collateral channels,relationship among emotions,menstruation and acne,and clinical effects,etc.this article emphasized treating acne from the liver theory and discussed its theoritical and clinical evidence.According to this artical,the liver fire attacking the lung was the important pathogenesis of the occurrence of acne,Eliminating the liver fire was the important method of treating acne.

This paper first described the concept of competitive intelligence and hospital competitive intelligence.Then,the resources of hospital library intelligence was analyzed with the method of SWOT,and the feasibility of competitive intelligence service was discussed.Finally,three aspects of the service were elaborated,which included collection of competitive intelligence,instruction of the knowledge of competitive intelligence,and the establishment of competitive intelligence system in hospitals.

Objective To evaluate the application of microcirculation temperature regulator for patient receiving reversed digital artery island flap surgery comparing with conventional heat lamp. Methods 68 patients after reversed digital artery island flap surgery in department of foot and hand surgery were randomized to treatment group using microcirculation temperature regulator (n=34) and control group using heat lamp (n=34) by random number table. Flap survival, length of hospital stay and satisfaction were evaluated. Results 100.00%of 34 flaps survived in the treatment group, higher than 67.65%(23 flaps of 34) in the control group, statistical differences were observed between two groups (χ2=13.123,P<0.01). Average length of hospital stay in treatment group was (11.6 ± 5.9) days, shorter than (19.5 ± 8.1) days in control group (t=4.600,P<0.01). Patients satisfaction rate was 100.00% (34/34) in microcirculation temperature regulator application group, which was also higher compared to using heat lamp 76.47% (26/34) (χ2=12.851,P<0.01). Conclusions Application of microcirculation temperature regulator could increase flap survival, shorten length of hospital stay, and improve patients′satisfaction.

Objective To study the effect of the products of entermorpha (EP), chlorella growth factor (CGF) and entermorpha dietary fiber (EPDF) on defecation and blood lipid regulation. Method Based on the composition of EP products, the male ICR mice were intervened with EP, EPDF and CGF with three doses. Using the constipation model induced by compound diphenoxylate, the effect of EP products on enterocinesia function of mice was determined by measuring defecation and the percentage of ink-pushing in small intestine. High-lipid rat model was established by feading high fat diet and the intervention effect with three doses of EPDF and CGF for 6 w was observed. Results As compared to the model group,all doses of EP,both middle and high doses of EPDF,and high dose of CGF could shorten the time of first defecation and increase the percentage of ink-pushing in small intestine of costive mice (P

Heat shock protein 27 is one of the members of the small heat shock protein family.Its molecular chaperone function and the interaction with the cell death pathway mediate cell survival under the stress state.The expression of heat shock protein 27 in ischemic lesions and remote areas and its changes in phosphorylation levels during cerebral ischemia is associated with neuronal tolerance to ischemia.However,the neuroprotective mechanism of heat shock protein 27 in cerebral ischemic tolerance still remains unclear.This article reviews the roles of heat shock protein 27 in cerebral ischemic tolerance and its potential mechanism.

Objective:To evaluate the wear resistance of four types of composite resin when they were opposed by human enamel antagonist. The relationship between pH value and wear resistance was also evaluated. Methods:Mutual wear between four types of composite resins(3 mm?20 mm?1 mm),Beautifit(B),SureFilTM(S),TE-Econom(T),Charisma (C),with same rough surface and opposing enamel was evaluated using a wear simulator of UMT-2 (n=10).Wear resistance was assessed as weight loss(g).The worn surfaces were observed with scanning electron microscope(SEM).All data were statistically analyzed with SPSS14.0.Results:SEM observation showed that the fillers particles of resin B,S were bigger than that of the others. Fillers of resin C was the smallest and the most well-distributed. Many filler particles were lost. In the acid environment,the enamel surface had the special appearance. The S,C,T composite resins were less worn. The weight loss of the T,B,S composite resins increased when pH value fell down to 4.0 (P0.05).Conclusion:The wear resistance among the different brands of composite resins are discrepant. The weight loss might be accelerated with the decreased pH value.

Objective To compare the molecular characteristics of hantanvirus strains isolated from 1980 to 2002 in Zhejiang Province by PCR and nucleotide sequencing.Methods Total RNA was extracted from hantanvirus infected Vero-E6 cells. The M segment cDNA of hantaan ZJ4 and ZJ7 strains were obtained by reverse transcription and polymerase chain reaction, subsequently cloned into pUCm-T vector and sequencing.Results Two strains ZJ4 and ZJ7 of hantanvirus isolated from Rottus in Zhejiang province were HTN viruses. HTN virus strain Z10 was isolated in 1980. Comparison ZJ4 and ZJ7 with Z10 strains indicated that there were 88.3% and 92.3% homology at the nucleotide level.Conclusion The HTN virus were very conserved. The homology of HTN virus isolated from Zhejiang Province was high nucleotide level, though they were isolated at more than 20-year intervals.

Objective To study the expression and clinical significance of miR-183-5p, TβRⅠ and TβRⅡ in esophageal squamous cell carcinoma (ESCC). Methods The mRNA and protein expression of miR-183-5p, TβRⅠ and TβRⅡ were examined in ESCC cell lines ECA-109, TE-1, normal esophageal epithelial cells, tumor tissues and tumor-free tissues from 72 ESCC patients. Their clinical significance and the relationship between miR-183-5p and the latter two were analyzed. The effects of miR-183-5p on the expression of TβRⅠand TβRⅡ in ECA-109 cells and the cell functions of ECA-109 were also investigated. Results Compared with the normal esophageal epithelia cells, ESCC cell lines TE-1 and ECA-109 were statistically characterized by a high expression of miR-183-5p (all P<0.05) and low expression of TβRⅠand TβRⅡ(all P<0.05). The expression of miR-183-5p in ESCC tissues was higher than that in adjacent normal tissues, while the expressions of TβRⅠ and TβRⅡ were lower (all P< 0.05). The expression of miR-183-5p was closely related to sex, tumor differentiation, tumor staging, distant metastasis, lymphatic metastasis, and tumor location (all P<0.05). TβRⅠlevel was associated with sex, lymph node metastasis and tumor size (all P<0.05). Experimental data showed the negative correlation between the expression of miR-183-5p and TβRⅠin ESCC tissues (r= -0.521, P< 0.05). Over expression of miR-183-5p significantly inhibited the expression of TβRⅠ in ECA-109 cells (P< 0.05) and promoted the growth, invasion and metastasis of ECA-109 cells (P< 0.05). Low expression of miR-183-5p significantly promoted the expression of TβRⅠ in ECA-109 cells (P< 0.05), and suppressed the growth, invasion and metastasis of ECA-109 cells (P< 0.05). There was no significant change in the expression of TβRⅡ in the transfection experiments. Conclusion MiR-183-5p is closely related to the abnormal expression of TβRⅠ, which may exert an important role in the progression of lymphatic metastasis.

ObjectiveTo observe the effects of ultrafiltration extract from Angelica Sinensis Radix and Hedysari Radix on PC12 cell apoptosis induced by H2O2; To discuss its mechanism of action.Methods H2O2 was used in the incubation of PC12 cells to establish the oxidative damage nerve cell apoptosis model. The experiment was divided into normal control group, model group, and three different dosages (0.375, 0.75, 1.5 g/L) of ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix after modeling for interference. Rate of cell apoptosis was detected by flow cytometry; mitochondrial membrane potential was measured by laser scanning confocal microscopy; the protein expressions of cleaved Caspase-3, Bax and Bcl-2 were detected by Western blot.Results Compared with the model group, rates of cell apoptosis in the different dosage groups decreased significantly; membrane potential of mitochondria increased; the protein expressions of cleaved Caspase-3 and Bax decreased; the expression of Bcl-2 increased; the ratio of Bcl-2 and Bax increased (P<0.05).Conclusion The ultra-filtration extracted from Angelica Sinensis Radix and Hedysari Radix can inhibit PC12 cell apoptosis induced by H2O2.