Objective: To monitor the quality and production procedure of Yiwei Capsules from various aspects. Methods: The method of HPLC gradient elution analysis was adopted to determine paeoniflorin, hesperidin, and glycyrrhizic acid of Yiwei Capsules at the same time. Results: Good results were obtained in recoveries and coefficient of variation of the three substances. Conclusion: In this method the pretreatment is simple and the operation is easy which is helpful in quick and accurate determination.

Objective To stuay the serum levels of sCD40L,hsCRP,ICAM-1 and VCAM-1,and the expression of CD40L of the CD4+T cells in patients withacute coronary syndrome(ACS),and to explore the relationship between CD40L and inflammatory factors and the effects of CD40/CD40L on ACS.Method Thirty-two coronary heart disease patients without history of other discernible systemic disease and medicine of steroids or immunosuppressants taken were divided into acute myocardial infarction group(AMI,n=11),unstable angina pectoris group(USP,n=14)and stable angina pectoris group(SAP,n=7).The control group was composed of eight healthy volunteers(CON group).Theexpression of CD40L Was determined by flow cytometry(FCM).Serum sCD40L.ICAM-1 and VCAM-1 were determined by using ELISA.The serum hsCRP was assayed by using immunoturbidimetry.Data were analyzed with SPSS 11.0 software for windows.Results The expression percentage(%)of CD40L of the CD4+T cells,and the serum levels of sCD40L,hsCRP,ICAM-1 and VCAM-1were sifnificantly higher in patients of AMI group than those in patients of other groups(P＜0.05 or P＜0.01).Similarly,those biomarkers in patients of UAP group were usually higher than those in patients of CON or SAP groups(P＜0.05).There Was a positive correlation between the expression of CD40L and the serum level of VCAM-1 in paients of AMI group(P＜0.05),and likewise,a positive correlation also existed between the serum level of sCD40L and other factors,hsCRP,ICAM-1 as well as VCAM-1,in patients of AMI group(P＜0.05).Conclusions The enhanced expression of CD40L of the CD4+T cells and high serum level of sCD40L are present in patients with acute coronary syndrome.The hsCRP,ICAM-1 and VCAM-1 play roles in the pathogenesis of ACS,and they have correlation with enhanced expression of CD40L and high serum level of sCD40L.Therefore,CD40L and sCD40L may be used as indicators of risk in coronary heart disease.

Objective: To investigate the clinicopathological characteristics and diagnosis of ovarian Brenner tumors. Methods: Forty-seven cases of ovarian Brenner tumors were enrolled from January 2012 to May 2018 at Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Clinical data, imaging examination, histopathological characteristics and immunohistochemical phenotype were analyzed. Results: The age of the patients ranged from 30-73 years and the mean age was 55 years. Thirty-nine patients (83.0%) were postmenopausal. Forty cases (85.1%) of the Brenner tumors were benign, five (10.6%) borderline and two (4.3%) malignant. Usual tumor markers of ovarian carcinoma, including CA199 and CA125 were normal or mild elevated in the 47 cases. Imaging before surgery was not specific to Brenner tumors. Microscopically, benign Brenner tumors were composed of nests of bland, transitional-type cells within a fibromatous stroma. In our 5 cases of borderline Brenner tumors, mildly atypical transitional-type cells were projected into the cyst lumens and lack of stromal invasion. In 2 cases of malignant Brenner tumors, different degrees of nuclear atypial transitional-type cells exhibited stromal invasion. Immunohistochemical stains for CK7, GATA3, p63 and CK5/6 were positive in all cases. Ki-67 was less than 5% in Brenner tumors, and up to 20%-30% in malignant Brenner tumors. Conclusion Brenner tumors are mostly seen in postmenopausal patients and are usually benign. Imaging examination and usual ovarian tumor markers do not provide diagnostic value. Diagnosis and classification of Brenner tumors depend on histopathological evaluation.

Objective To explore the values of translocator protein(TSPO)and serum cell-free mitochondrial DNA(cf-mtDNA)in predicting the disease condition and prognosis of patients with trau-matic shock.Methods Eighty patients(traumatic shock group)with traumatic shock and eighty pa-tients(without traumatic shock group)without traumatic shock were selected.Complete demographic and clinical laboratory data of patients were collected.Blood samples of patients with traumatic shock were collected at the time points of immediately after admission(T,)and the first day(T2),the third day(T3)and seventh day(T4)after admission,the level of TSPO was measured by enzyme-linked immunosorbent assay(ELISA),and level of cf-mtDNA was measured by quantitative real-time poly-merase chain reaction(qPCR).The levels of TSPO and cf-mtDNA were compared between patients with and without traumatic shock;the patients with traumatic shock were divided into the poor progno-sis group and good prognosis group according to differed prognostic outcome,and the levels of TSPO and cf-mtDNA were compared between the two groups.The predictive values of TSPO and cf-mtDNA for the prognosis of patients with traumatic shock were analyzed by the receiver operating characteristic(ROC)curve.Results Compared with the no traumatic shock group,the traumatic shock group had higher levels of serum TSPO at T,to T4 and higher levels of cf-mtDNA at T2 to T3,and the differences were statistically significant(P＜0.05).Compared with the good prognosis group,the poor prognosis group had higher levels of serum TSPO and cf-mtDNA at T,to T4,and the differences were statisti-cally significant(P＜0.05).Taking the prognosis of traumatic shock patients as the state variable to perform ROC curve analysis,the results showed that the area under the curve(AUC)of TSPO from T1 to T4 for predicting prognosis in the poor prognosis group was 0.825,0.829,0.695 and 0.869 respec-tively,while those of cf-mtDNA level from T1 to T4 for predicting prognosis in the poor prognosis group was 0.766,0.766,0.837 and 0.783 respectively,and the differences were statistically significant(P＜0.001).Conclusion Traumatic shock patients have elevated levels of TSPO and cf-mtDNA,with significantly higher levels observed in those with poor prognosis,and the TSPO level on the sev-enth day and cf-mtDNA level on the third day have the highest predictive value for prognosis.

Objective To explore the values of translocator protein(TSPO)and serum cell-free mitochondrial DNA(cf-mtDNA)in predicting the disease condition and prognosis of patients with trau-matic shock.Methods Eighty patients(traumatic shock group)with traumatic shock and eighty pa-tients(without traumatic shock group)without traumatic shock were selected.Complete demographic and clinical laboratory data of patients were collected.Blood samples of patients with traumatic shock were collected at the time points of immediately after admission(T,)and the first day(T2),the third day(T3)and seventh day(T4)after admission,the level of TSPO was measured by enzyme-linked immunosorbent assay(ELISA),and level of cf-mtDNA was measured by quantitative real-time poly-merase chain reaction(qPCR).The levels of TSPO and cf-mtDNA were compared between patients with and without traumatic shock;the patients with traumatic shock were divided into the poor progno-sis group and good prognosis group according to differed prognostic outcome,and the levels of TSPO and cf-mtDNA were compared between the two groups.The predictive values of TSPO and cf-mtDNA for the prognosis of patients with traumatic shock were analyzed by the receiver operating characteristic(ROC)curve.Results Compared with the no traumatic shock group,the traumatic shock group had higher levels of serum TSPO at T,to T4 and higher levels of cf-mtDNA at T2 to T3,and the differences were statistically significant(P＜0.05).Compared with the good prognosis group,the poor prognosis group had higher levels of serum TSPO and cf-mtDNA at T,to T4,and the differences were statisti-cally significant(P＜0.05).Taking the prognosis of traumatic shock patients as the state variable to perform ROC curve analysis,the results showed that the area under the curve(AUC)of TSPO from T1 to T4 for predicting prognosis in the poor prognosis group was 0.825,0.829,0.695 and 0.869 respec-tively,while those of cf-mtDNA level from T1 to T4 for predicting prognosis in the poor prognosis group was 0.766,0.766,0.837 and 0.783 respectively,and the differences were statistically significant(P＜0.001).Conclusion Traumatic shock patients have elevated levels of TSPO and cf-mtDNA,with significantly higher levels observed in those with poor prognosis,and the TSPO level on the sev-enth day and cf-mtDNA level on the third day have the highest predictive value for prognosis.

Objective To investigate the clinicopathological characteristics and diagnosis of ovarian Brenner tumors. Methods Forty?seven cases of ovarian Brenner tumors were enrolled from January 2012 to May 2018 at Beijing Obstetrics and Gynecology Hospital, Capital Medical University. Clinical data, imaging examination, histopathological characteristics and immunohistochemical phenotype were analyzed. Results The age of the patients ranged from 30-73 years and the mean age was 55 years. Thirty?nine patients (83.0%) were postmenopausal. Forty cases (85.1%) of the Brenner tumors were benign, five (10.6%) borderline and two (4.3%) malignant. Usual tumor markers of ovarian carcinoma, including CA199 and CA125 were normal or mild elevated in the 47 cases. Imaging before surgery was not specific to Brenner tumors. Microscopically, benign Brenner tumors were composed of nests of bland, transitional?type cells within a fibromatous stroma. In our 5 cases of borderline Brenner tumors, mildly atypical transitional?type cells were projected into the cyst lumens and lack of stromal invasion. In 2 cases of malignant Brenner tumors, different degrees of nuclear atypial transitional?type cells exhibited stromal invasion. Immunohistochemical stains for CK7, GATA3, p63 and CK5/6 were positive in all cases. Ki?67 was less than 5% in Brenner tumors, and up to 20%-30% in malignant Brenner tumors. Conclusion Brenner tumors are mostly seen in postmenopausal patients and are usually benign. Imaging examination and usual ovarian tumor markers do not provide diagnostic value. Diagnosis and classification of Brenner tumors depend on histopathological evaluation.

OBJECTIVETo evaluate the rationality of T staging of gastric cancer with transverse mesocolon invasion.

METHODSData of 808 patients with primary gastric cancer undergoing surgical treatment was screened from the Data base of Gastric Cancer of Sun Yat-sen University, from April 1996 to October 2009. According to the information of transverse mesocolon invasion, all cases were divided into groups NOI (T4a stage, non organ invasion, n = 638), NTMI (T4b stage, non transverse mesolon invasion, with organ invasion, n = 126), and TMI (transverse mesocolon invasion, n = 44). The clinicopathological features, surgical procedure and prognosis were compared among the three groups.

RESULTSNo significant difference was found in gender, age, lymph node metastasis, hepatic metastasis, tumor's Borrmann type, histological type, differentiation degree, value of serum CEA among the 3 groups (all P > 0.05). In the groups NOI, NTMI and TMI, the ratio of mean tumor diameter ≥ 5 cm was 39.0% (249/638), 61.1% (77/126) and 54.5% (24/44), respectively; the ratio of distal metastasis was 11.9% (76/638), 30.2% (38/126) and 43.2% (19/44), respectively; the ratio of peritoneal metastasis was 8.2% (52/638), 26.2% (33/126) and 38.6% (17/44), respectively; the ratio of TNM IV stage was 25.4% (162/638), 84.7% (107/126) and 93.7% (41/44), respectively; and the ratio of radical resection was 92.0% (587/638), 69.8% (88/126) and 77.3% (34/44), respectively; all with significant differences (P < 0.01), and the results of pairwise comparisons (Bonferroni correction, significant level α = 0.05/3 = 0.0167) showed that these parameters were significantly different between groups NOI and TMI (P < 0.0167), but non-significant between groups NTMI and TMI (P > 0.0167). The median survival time was 42.0, 16.4 and 19.0 months in the groups NOI, NTMI and TMI, respectively (P < 0.01), and the results of pairwise comparison showed that the prognosis were significant different between the groups NOI and TMI (P < 0.01), but non-significant between the groups NTMI and TMI (P > 0.05). In the cases who received radical resection, the median survival time was 47.9, 23.5 and 21.4 months in the groups NOI, NTMI and TMI, respectively (P < 0.01), and the results of pairwise comparison showed that the prognosis was significantly different between the groups NOI and TMI (P < 0.05), but not significant between groups NTMI and TMI (P > 0.05).

CONCLUSIONSThe tumor size, distal meatastasis, peritoneal metastasis, TNM stage, surgical procedure and prognosis of gastric cancer with transverse mesocolon invasion are similar to that of T4b gastric cancer, but are significantly different from that of T4a gastric cancer. Gastric cancer with transverse mesocolon invasion should be reclassified as T4b stage.

Colonic Neoplasms ; pathology ; Humans ; Mesocolon ; pathology ; Neoplasm Staging ; Stomach ; pathology ; Stomach Neoplasms ; pathology