HCMV infection can cause hepatopathy,pneumonia,diarrhea,thrombocytopenic purpura,and sensorineural hearing loss(SNHL),with high morbility and mortality.pp65(phosphoprotein 65),a major constitutive protein of virion,is closely associated with viral gene expression,immune evasion and cell metabolism.pp65 antigenemia level has positive correlation with clinical symptom,which can guide the preemptive therapyand improve prognosis,is considered as one of the golden standard methods of active cytomegalovirus infection.In this paper,the pp65 protein properties,pathogenesis,application in diagnosis and prevention are reviewed.

Objective To explore the feelings of the caregivers of dementia patients,and find the resource of the caregiver's abuse tendency to dementia patients in community.Methods The data of 10 caregivers were collected through deep interviews,and the Colaizzi analysis method was used to analyze the data.Results Through repeatedly reading,arranging,analysis,separation,and theme refining,five themes were drawn:lack of related knowledge and care skills,care tasks making caregivers tired,the economic burden,lack of social support,low sense of worth.Conclusions When concerning the problems caused by dementia patients,it's necessary to appeal the society and government to provide feasible service and assistance on the issue of abuse tendency,which is produced by the caregivers during the process of taking care of them.

Recent researches have shown that gut microbiota play an important role in pathogenesis of immune-related disorders.Type 1 diabetes mellitus,as an autoimmune disease,has received increasing attention,especially in terms of the relationship between the pathogenesis and intestinal microbiota.Change of gut microbiota is an important risk factor of the pathogenesis of type 1 diabetes mellitus which have been confirmed with the animal model studies.This article reviews the role of gut microbiota in the pathogenesis of type 1 diabetes mellitus,and the influence of gut microbiota on host immune response for possible prevention and treatment of type 1 diabetes mellitus in the future.

The ultrastructures of synapses in the head of caudate nuclei of rats were studied by means of electron microscopic technique. The experimental group of rats, in which the pain threshold was elevated after electro-acupuncture, showed no change of the number of the granular vesicles, but a significant decrease of the number of clear synaptic vesicles (without dense core) in the presynaptic boutons. This might be correlated to release of acetylcholine in the synapse.

The stimulate effects of synthetical endogenous peptides, tuftsin (Tuf) and thymopentin(TP5), on phagocytic function were studied. After subcutaneous administration (sc) of Tuf, thelysozyme content in both M? and sera, and the acid phosphatase activity in M?, were increasedin a dose-dependent manner. The peak time of serum lysozme level following a sigle sc injectionof Tuf was about on the 6th day. The elevation of lysozyme in sera was delayed and enhancedstrongly by Freund incomplete adjuvant (FIA). In cylcophosphamide (Cy) treated mice,TP5further increased both lysozyme content amd acid phosphatase activity in M?activated byCy, and recovered the lysozyme level in spleen inhibited by cy. The results suggested thatTP5could not only activate the funceion of M?, but recover the number of M?decreased by im-mumoimhibitant.

OBJECTIVE:To explore the clinical efficacy and safety of Yunkang granule combined with dydrogesterone in the treatment of early threatened abortion. METHODS:128 patients with early threatened abortion were randomly divided into single drug group and combined medication group,64 cases in each group. Single drug group received Dydrogesterone tablet with first dose of 40 mg,then 10 mg,q 12 h until vaginal bleeding;combined medication group additionally received 8 g Yunkang granule, tid,until symptoms disappeared. Human chorionic gonadotropin (HCG),clinical symptoms remission time and disappearance time,pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcome,and the incidence of adverse reac-tions in 2 groups were compared. RESULTS:After treatment,HCG in 2 groups significantly increased,and combined medication group was higher than single drug group,the differences were statistically significant(P<0.05);clinical symptoms remission time and disappearance time in combined medication group were shorter than single drug group,the difference was statistically signifi-cant (P<0.05). There were no significant differences in incidence of pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcomes in 2 groups (P>0.05). The incidence of adverse reactions in combined medication group was 10.9%, which was significantly lower than single drug group (40.6%),the difference was statistically significant (P<0.05). CONCLU-SIONS:Both dydrogesterone alone and Yunkang granule combined with dydrogesterone show good efficacy in the treatment of ear-ly threatened abortion,but the remission time and disappearance time of clinical symptoms and adverse reactions in combined medi-cation group are less than that in single drug group.

Objective To study the clinical effectiveness analysis of early use of low molecular weight hepa-rin and aspirin in the treatment of acute cerebral infarction.Methods 120 cases with acute cerebral infarction were randomly divided into the observation group and the control group according to a random number table,with 60 cases in each group.The patients of the control group were treated with aspirin,while the patients of the observation group were given low molecular weight heparin on the basis of the control group.Both groups were treated for one month,and the therapeutic effects and adverse reactions of the two groups were compared.Results The total efficiency of the ob-servation group was 96.7%(58 /60),which was significantly higher than the 76.7%(46 /60)of the control group, the difference was statistically significant(χ2 =12.051,P =0.019);The neurological deficits after treatment of the observation group was (16.21 ±2.05 )points,which was significantly lower than (26.04 ±1.03)points of the control group,the difference was statistically significant (t =10.372,P =0.027 ).Conclusion Acute cerebral infarction early use of low molecular weight heparin and aspirin had better clinical efficacy,and it can significantly promote the recovery of neurological function in patients.

Objective To develop a rapid ,convenient ,sensitive and specific method for the detection of marine Vbrio vulnificus by using loop‐mediated isothermal amplification(LAMP) technique ,and evaluate the specificity and sensitivity of this method . Methods According to marine Vibrio vulnificus cytolysin(vvhA) gene sequence published by GenBank ,a set of LAMP primers were designed .LAMP and polymerase chain reaction(PCR) amplification were carried out in 47 strains of bacteria(including 20 strains of Vibrio) and specificities of LAMP and PCR were compared .Serial ten‐fold dilutions of an overnight Vibrio vulnificus M06 culture were prepared in sterile saline solution ,and compared the sensitivity of LAMP with that of PCR after extracting DNA tem‐plates .A recombinant plasmid containing fragment of vvhA gene was constructed and set as a standard positive control of LAMP assay .Results False‐positive results occurred in the conventional PCR ,while in the LAMP assay positive amplifications were only seen in strains of Vibrio vulnificus and no false‐positive or false‐negative results were generated among 47 strains of bacteria ,which indicated that primers had high specificity .Additionally ,the results of electrophoresis were consistent with those after adding the calcein .The detection limit of LAMP was 4 × 10 CFU each reaction for detecting vvhA gene in pure culture ,which was 10‐fold more sensitive than that of conventional PCR(4 × 102 CFU each reaction) .It indicated that LAMP assay had good sensitivity .Repeated the test twice ,the detection results of LAMP and PCR were both stable .Conclusion An LAMP detection method for marine Vibrio vulnificus has been developed ,which is highly specific ,sensitive ,convenient and suitable for rapid field detection and point‐of‐care testing .

Objective To evaluate the clinical application value of the HC-900 fully automated urine dry chemistry analyzer and its matched test strip (HC-900 analysis system) for detecting urine microalbumin (U-mAlb) .Methods 660 urine samples were collected with the negative urine protein detected by the HC-900 analysis system as the standard ,among them ,159 samples with positive U-mAlb screened by the test strip and 106 samples with the partial negative were performed the quantitative analysis by the Immage 800 fully automatic specific protein analyzer for verifying the results screened by the HC-900 analysis system .The compari-son of the U-mAlb quantitative detection results between the screening positive group and the screening negative group adopted the two samples rank sum test .The comparison between the enumeration data was carried out by Kappa test for consistency .Results Among 660 samples ,159 samples with positive U-mAlb were quantitatively detected by the Immage 800 analyzer and 101 samples were confirmed positive U-mAlb with the real positive rate of 63 .5% .Among 106 samples of negative U-mAlb randomly extracted by the HC-900 analysis system ,9 samples were confirmed to be positive U-mAlb quantitatively detected by the Immage 800 analy-zer .By the consistency test ,the difference between the two methods had statistical significance (Kappa=0 .495 ,P<0 .01) .The U-mAlb level was 18 .02(8 .23-34 .89)mg/L in the screening positive group ,which was significantly higher than 4 .78(2 .51-8 .46) mg/L in the screening negative group ,the difference had statistical significance (Z= -8 .689 ,P<0 .01) .Relative to the detection by the Immage 800 quantitative analyzer ,the sensitivity and specificity of the U-mAlb for the rapid screening by the dry chemistry method was 91 .8% (101/110) and 62 .5% (97/155) respectively .Conclusion The HC-900 analysis system for screening U-mAlb has a certain clinical application value .