HCMV infection can cause hepatopathy,pneumonia,diarrhea,thrombocytopenic purpura,and sensorineural hearing loss(SNHL),with high morbility and mortality.pp65(phosphoprotein 65),a major constitutive protein of virion,is closely associated with viral gene expression,immune evasion and cell metabolism.pp65 antigenemia level has positive correlation with clinical symptom,which can guide the preemptive therapyand improve prognosis,is considered as one of the golden standard methods of active cytomegalovirus infection.In this paper,the pp65 protein properties,pathogenesis,application in diagnosis and prevention are reviewed.

The ultrastructures of synapses in the head of caudate nuclei of rats were studied by means of electron microscopic technique. The experimental group of rats, in which the pain threshold was elevated after electro-acupuncture, showed no change of the number of the granular vesicles, but a significant decrease of the number of clear synaptic vesicles (without dense core) in the presynaptic boutons. This might be correlated to release of acetylcholine in the synapse.

Objective To explore the feelings of the caregivers of dementia patients,and find the resource of the caregiver's abuse tendency to dementia patients in community.Methods The data of 10 caregivers were collected through deep interviews,and the Colaizzi analysis method was used to analyze the data.Results Through repeatedly reading,arranging,analysis,separation,and theme refining,five themes were drawn:lack of related knowledge and care skills,care tasks making caregivers tired,the economic burden,lack of social support,low sense of worth.Conclusions When concerning the problems caused by dementia patients,it's necessary to appeal the society and government to provide feasible service and assistance on the issue of abuse tendency,which is produced by the caregivers during the process of taking care of them.

Recent researches have shown that gut microbiota play an important role in pathogenesis of immune-related disorders.Type 1 diabetes mellitus,as an autoimmune disease,has received increasing attention,especially in terms of the relationship between the pathogenesis and intestinal microbiota.Change of gut microbiota is an important risk factor of the pathogenesis of type 1 diabetes mellitus which have been confirmed with the animal model studies.This article reviews the role of gut microbiota in the pathogenesis of type 1 diabetes mellitus,and the influence of gut microbiota on host immune response for possible prevention and treatment of type 1 diabetes mellitus in the future.

Objective To evaluate the efficacy and safety of the combination of Weimaining capsule and XELOX regimen (oxaliplatin plus capecitabine) in the treatment of advanced gastric cancer patients.Methods A total of 62 patients with advanced gastric cancer who fulfilled all predetermined criteria were randomly divided into observation group and control group.The 31 patients in observation group received a combination of Weimaining capsule and XELOX regimen,the 31 patients in control group received only XELOX regimen at the same dose intensity.Each patient received at least two cycles (1 cycle =21 days) of treatment,the efficacy was assessed after two cycles.Results The median time-to-progression (TTP) of observation and control groups were 5.5 and 4.8 months,respectively.The difference had no statistical significance (P =0.238).The disease control rates (DCR) of observation group was significantly improved compared with control group [67.7 ％ (21/31) vs 41.9 ％ (13/21),P ＜ 0.05],the difference was statistically significant,while the objective response rate (ORR) of the 2 groups had no statistical difference [(32.3 ％ (10/31) vs 25.8 ％ (8/31),P ＞ 0.05].In drug safety aspects,there was no Ⅴ grade adverse reactions (deaths caused by drug) among all the patients.Rates of grades Ⅱ-Ⅳ neutrocytopenia and nausea,vomiting in observation group were obviously lower than control group [16.13 ％ (5/31) vs 38.71％ (12/31),P ＜ 0.05;12.90 ％ (4/31) vs 35.48 ％ (11/31),P ＜ 0.05],other adverse reactions between the 2 groups had no statistical difference.Conclusions A combination of Weimaining capsule and XELOX regimen in the treatment of advanced gastric cancer patients can improve DCR significantly and reduce part of adverse reaction induced by chemotherapy with satisfactory safety.This method is worthy of application widely.

The stimulate effects of synthetical endogenous peptides, tuftsin (Tuf) and thymopentin(TP5), on phagocytic function were studied. After subcutaneous administration (sc) of Tuf, thelysozyme content in both M? and sera, and the acid phosphatase activity in M?, were increasedin a dose-dependent manner. The peak time of serum lysozme level following a sigle sc injectionof Tuf was about on the 6th day. The elevation of lysozyme in sera was delayed and enhancedstrongly by Freund incomplete adjuvant (FIA). In cylcophosphamide (Cy) treated mice,TP5further increased both lysozyme content amd acid phosphatase activity in M?activated byCy, and recovered the lysozyme level in spleen inhibited by cy. The results suggested thatTP5could not only activate the funceion of M?, but recover the number of M?decreased by im-mumoimhibitant.

In order to evaluate the effect of chinese medicine (Yi Qi Xiao Zheng decoction) in reducing radiation side effect, an observation of peripheral blood WBC, stem cell amount, the function of liver and renal in mice was made during and after radiation.The results showed that WBC and stem cell amount were higher in Chinese medicine combined with radiation group than radiation only group after 3 fraction radiation(P

Objective To develop a rapid ,convenient ,sensitive and specific method for the detection of marine Vbrio vulnificus by using loop‐mediated isothermal amplification(LAMP) technique ,and evaluate the specificity and sensitivity of this method . Methods According to marine Vibrio vulnificus cytolysin(vvhA) gene sequence published by GenBank ,a set of LAMP primers were designed .LAMP and polymerase chain reaction(PCR) amplification were carried out in 47 strains of bacteria(including 20 strains of Vibrio) and specificities of LAMP and PCR were compared .Serial ten‐fold dilutions of an overnight Vibrio vulnificus M06 culture were prepared in sterile saline solution ,and compared the sensitivity of LAMP with that of PCR after extracting DNA tem‐plates .A recombinant plasmid containing fragment of vvhA gene was constructed and set as a standard positive control of LAMP assay .Results False‐positive results occurred in the conventional PCR ,while in the LAMP assay positive amplifications were only seen in strains of Vibrio vulnificus and no false‐positive or false‐negative results were generated among 47 strains of bacteria ,which indicated that primers had high specificity .Additionally ,the results of electrophoresis were consistent with those after adding the calcein .The detection limit of LAMP was 4 × 10 CFU each reaction for detecting vvhA gene in pure culture ,which was 10‐fold more sensitive than that of conventional PCR(4 × 102 CFU each reaction) .It indicated that LAMP assay had good sensitivity .Repeated the test twice ,the detection results of LAMP and PCR were both stable .Conclusion An LAMP detection method for marine Vibrio vulnificus has been developed ,which is highly specific ,sensitive ,convenient and suitable for rapid field detection and point‐of‐care testing .

OBJECTIVE:To explore the clinical efficacy and safety of Yunkang granule combined with dydrogesterone in the treatment of early threatened abortion. METHODS:128 patients with early threatened abortion were randomly divided into single drug group and combined medication group,64 cases in each group. Single drug group received Dydrogesterone tablet with first dose of 40 mg,then 10 mg,q 12 h until vaginal bleeding;combined medication group additionally received 8 g Yunkang granule, tid,until symptoms disappeared. Human chorionic gonadotropin (HCG),clinical symptoms remission time and disappearance time,pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcome,and the incidence of adverse reac-tions in 2 groups were compared. RESULTS:After treatment,HCG in 2 groups significantly increased,and combined medication group was higher than single drug group,the differences were statistically significant(P<0.05);clinical symptoms remission time and disappearance time in combined medication group were shorter than single drug group,the difference was statistically signifi-cant (P<0.05). There were no significant differences in incidence of pregnancy/childbirth complications,pregnancy outcome and neonatal adverse outcomes in 2 groups (P>0.05). The incidence of adverse reactions in combined medication group was 10.9%, which was significantly lower than single drug group (40.6%),the difference was statistically significant (P<0.05). CONCLU-SIONS:Both dydrogesterone alone and Yunkang granule combined with dydrogesterone show good efficacy in the treatment of ear-ly threatened abortion,but the remission time and disappearance time of clinical symptoms and adverse reactions in combined medi-cation group are less than that in single drug group.