Objective To investigate the dose fraction of gamma knife treatment brain deep larger glioma clinical efficacy.Methods To adopt rotating gamma knife in dose fraction treatment of brain deep larger(d＞3 cm),64 cases of gliomas,were installed stereotactic head frame can repeat accessories,for a day,for two consecutive days to five,each around 5-10 Gy for each treatment,fixed the first-place after the removal,the next treatment repeat.ReslIlts After 6 to 24 months of follow-up was 57 patients,complete response(CR)in 8 cases,partial response(PR)in 29 cases,the total efficiency of 64.9%,no change(NC)in 14 cases,clinical benefit rate of 83.4%,progressive disease(PD)in 6 cases.Brain edema response after treatment was the major side effects.Conclusion For deep larger brain glioma,dose fraction of gamma knife treatment was clearly effective recently,it could ease symptoms and improve quality of life.

Objective To investigate the right ventricular function of patients with chronic pulmonary hypertension using Doppler tissue imaging(DTI)and to evaluate the relationship between DTI parameters and pulmonary artery systolic pressure(PASP).Methods Thirty-five patients with chronic pulmonary hypertension and thirty-five healthy subjects underwent Doppler tissue echocardiographic evaluation of right ventricular function.The tricuspid lateral annular systolic velocity(Sm),early(Em)and late(Am)peak diastolic velocities,isovolumic contraction time(IVCT)and isovolumic relaxation time(IVRT)were acquired from apical 4-chamber view.Results When compared with control group,Sm,Ea and Em/Am values were found to be lower and Am,IVRT values higher in patients with chronic pulmonary hypertension.When all patients were analyzed,there was a significant positive correlation between PASP and IVRT(r=0.78,P

Objective To study the immunophenotype and chromosome karyotype of 57 adult patients with newly?diagnostic acute lymphoblastic leukemia(ALL). Methods The immunophenotype and chromosome karyotype of 57 adult patients with newly?diagnostic ALL were determined by using flow cytometer and karyotyping,and then the clinical characteristics and significance were analyzed. Results In patients with B cell acute lymphoblastic leukemia(B?ALL),the expression of CD19 and CD22 were significantly higher than CD10 and CD20(P<0.05). The expression of CD7 and CD3 were much higher in patients with T cell acute lymphoblastic leukemia(T?ALL). As for the early antigen,patients of all subtypes of ALL showed high expression rate of CD34 and CD38. In addition,the HLA?DR was only detected in B?ALL. There was no difference in comparison with clinical characteristics,total rate of CR and rate of relapse between ALL with and without myeloid antigen expression. The expression rate of CD34 and HLA?DR was higher in My+?ALL(P<0.05). The relapse rate was much higher in these patients with abnormal karyotype than other pa?tients(P<0.05). Patients with high?risk karyotype showed higher expression rates of CD20 and CD3 than those with standard?risk. Conclusion The immunophenotypes and chromosome karyotypes show obviously heterogeneous features,and the patients with abnormal karyotypes have higher relapse rate. These above mentioned methods are applicable for the diagnosis and individualized treatment of ALL patients.

Objective To investigate the ratios and the distribution of subjects with indeterminate results of HIV antibody in different populations who were positive for HIV screening test in Fujian and to ana-lyze the features of Western blot bands for further understanding the relationships between the Western blot band patterns and HIV infection. Methods The subjects who were diagnosed with indeterminate results of HIV antibody at the first examination were re-tested with Western blot assay every month until the results turned into HIV antibody-positive or HIV antibody-negative. Results The detection rate of indeterminate results of HIV antibody was 3. 69% in Fujian in 2015. Higher rates were detected in blood donors, pregnant woman and other clinical patients. The most common band patterns of HIV antibody-indeterminate samples were p24, gp160+p24 and gp160. Results of the follow-up confirmatory tests showed that 76% of the sam-ples of p24 band pattern were HIV antibody-negative and 76. 47% of the samples of gp160+p24 band pattern were HIV antibody-positive. The nucleic acid test showed that positive results were detected in 29 samples whose band patterns were gp160+gp120+p24,gp160+gp120+p66andgp160+gp120+p31. Conclusion The detection rates of indeterminate results of HIV antibody varied in different populations. The most com-mon band patterns of HIV antibody-indeterminate samples were p24, gp160+p24 and gp160. The more bands the samples presented themselves in Western blot assay, the more likely they would be HIV antibody-positive. Follow-up should be strengthened to prevent the spread of HIV infection. Samples with the band pattern of gp160+gp120+p24 showed a low probability of false positive and they were more likely to be end-stage AIDS with immunosuppression.

Objective To study the effects of antimicrobial peptide LL-37 expressed and purified from prokaryotes on candida albicans growth. Methods (1) Thirty female Kunming mice were treated with estrogen and white candida yeast suspension were poured into vagina to establish a vulvovaginal candidiasis (VVC) murine model. After successful establishing the VVC mouse model, mice were randomly sorted into test group (n=15) and control group (n=15) . Suspension(30μl, 100μg/ml)of recombinant peptide LL-37 expressed and purified in Prokaryotes was given by intravaginal administration to the test group for 5 days, while the same amount of phosphate buffered saline (PBS) was given to the control group. (2) Tweenty-four hours after treatment, the fungal burden and colony-forming unit (CFU) of vaginal fluids were evaluated. All mice were subsequently sacrificed and vaginal tissues were harvested for tissue homogenate preparation. ELISA was used to determine the levels of nterleukin-10(IL-10)and interferon-γ(IFN-γ)in the isolated vaginal tissues. Results (1) VVC mouse model was established successfully in this study. Vaginal mucosa congestion, edema, vaginal plica disappearing were obviously observed in the control group. After treatment with recombinant protein LL-37 vaginal mucosa has no obvious change in the test group. (2) Fungal burden and CFU of vaginal fluids were significantly lower in the test group [(4.8±1.0)×104 CFU/ml] than that in the control group [(8.5 ± 2.1) × 104 CFU/ml, P=0.017]. IFN-γlevel of the test group was increased [(257 ± 11) vs (197 ± 4) pg/ml, P=0.000], while the level of IL-10 was reduced [ (287 ± 15) vs (379 ± 17) pg/ml P=0.000] resulting in a the ratio of IFN-γ/IL-10 was in significantly higher in test group (0.892±0.008 vs 0.496±0.013, P=0.000). Conclusion Recombinant protein LL-37 expressed and purified from prokaryotes inhibits the growth candida albicans and improves vaginal immunity by adjusting IFN-γand IL-10 secretion in the VVC mouse model, highlighting the therapeutic potential of LL-37 for VVC.

Objective To construct the prokaryotic expression vector for HD-5 and purify the recombinant HD-5 protein then analyze its antifungal activity. Methods The HD-5 gene was cloned by PCR, then was inserted into prokary-otic expression plasmid pQE-30Xa to construct pQE-30Xa/HD-5. After sequencing, pQE-30Xa/HD-5 was transformed in-to E.coli M15 cells. Its expression was induced by IPTG and confirmed by SDS-PAGE. The recombinant protein was purified through Ni-NTA affinity purification system. The antifungal activity was tested by disk diffusion method. Results HD-5 gene and pQE-30Xa/HD-5 vector were obtained successfully. E.coli M15 strains was used to express HD-5 fusion protein. After purification, the fusion protein was confirmed by Western blot. The disk diffusion test confirmed that the fusion pro-tein can inhibit Candida albicans. Conclusion Expression vector pQE-30Xa/HD-5 was successfully constructed. The HD-5 fusion protein was expressed in E.coli successfully, which showed a certain degree of antifungal activity.

Objective To investigate the effects of almitrine-raubasine on learning-memory ability and brain choline acetyltransferase (ChAT) activity in chronic episodic hypoxia (EHYP) rat. Methods After establishing the rat model of EHYP, almitrine-raubasine (0.03 tablets/250 g body weight , Bid) was given to the EHYP rats. The learning-memory ability was evaluated by using passive avoidance test and the ChAT activity in three different brain regions (including cerebral cortex, hippocampus and striatum) was determined using radiochemical method. Results As compared with the controlled rats, the performance on passive avoidance test of EHYP rats was impaired significantly (P

Objective To investigate the impact of workplace bullying,general self?efficacy,self?es?teem on mental health of nurses. Methods With random sampling,450 nurses from six hospitals in Nanning of Guangxi were selected to complete questionnaire survey. Results 34.7% of nurses were reported to have been subjected to bullying at their workplace. Workplace bullying(23.78±6.94) showed significantly nega?tive relationship with mental health(36.63±4.53)( r=-0.32, P<0.01),while general self?efficacy(26.86± 4.82) and self?esteem(30.62±4.08) showed significantly positive correlations with mental health( r=0.45, 0.44, P<0.01). Workplace bullying had a significantly negative effect on mental health ( β=-0.25, P<0.01) ,and the relationship was affected by general self?efficacy and self?esteem. Conclusion Workplace bullying can influent nurses' mental health through self?esteem and the relationship is moderated by general self?efficacy.

OBJECTIVEThis study aims to investigate the color stability of ceromer with different thicknesses and different types of resin adhesive materials after accelerated aging and provide references for clinical application and selections.

METHODSNine groups of experimental samples were used, and each group contained five samples. We made joint samples with ceromer having three different thicknesses (1.00, 0.75, 0.50 mm) combined with three different resin adhesive materials (RelyX Veneer, RelyX Unicem, Filtek Z350 Flow), respectively. All samples were placed into Xenon Lamp Aging Instrument to implement accelerated aging. Spectrophotometer was used to measure the lightness (L*), red green color value (a*), and blue yellow color value (b*) of all samples before and after accelerated aging. The change of lightness (ΔL), red green color value (Δa), blue yellow color value (Δb), and color variation (ΔE) were also calculated. We investigated the influence of ceromer veneer thicknesses and resin adhesive material types on color variation by two-factor analysis of variance.

RESULTSThe thickness and type factors showed significant influence on ΔE values, and exhibited interactions (P < 0.05). The ΔE values of all experimental groups were lower than 3.3. After the accelerated aging process, all L*, a*, and b* values of the experimental groups decreased and the ΔL values were lower than 2.0.

CONCLUSIONCeromer veneer thickness and resin adhesive material types could affect the color stability of ceromer veneer and resin adhesive materials. The changes in lightness and color in ceromer veneer and resin adhesive materials are considered clinically acceptable after accelerated aging.

OBJECTIVETo determine the effect of type I transmembrane protein (IRE1alpha) deletions on the cell cycle of human periodontal ligament fibroblasts (hPDLFs) cells.

METHODSBased on the IRE1alpha deletions, a full-length model was successfully constructed. Moreover, overlapping polymerase chain reaction mutagenesis facilitated the establishment of two deletion mutants of IREla (pD-Kinase, pD-Rnase). The full-length model and two mutant eukaryotic expression vectors were transfected into hPDLFs cells. Western blot analysis was performed to identify the expression in the cells. The changes in the cell cycle of hPDLFS cells were detected by flow cytometry (FCM).

RESULTSThe two deletion mutants of IRE1alpha with eukaryotic expression vectors were successfully constructed and correctly expressed in hPDLFs cells based on Western blot analysis. Under stress conditions, the FCM assay showed that cell percentage of S phases increased, whereas that of G1 phases decreased in the IRE1alpha group (P < 0.05) compared with the control group of tunicamycin (TM) treatment. Moreover, the cell percentage of the S phases decreased, whereas that of the G1 phases increased in the D-Rnase group (P < 0.05) compared with the control. The deletion mutant D-Kinase had no influence on hPDLFS cell proliferation and cycle (P>0.05).

CONCLUSIONUnder stress conditions, IRE1alpha can improve the cell cycle of hPDLFs cells from the G1 to the S phase. The deletion mutant D-Rnase cause hPDLFs cell growth arrest at the G1 phase, whereas deletion mutant D-Kinase has no significant effect.