With the development of proteomics, the application in the research for the function of proteins becomes popular. This review article presents that proteomics, a kind of tools of molecular biology, has been successfully used for studying the pathogenesis of type 1 and type 2 diabetes.

Objective To analyze the clinical and pathological characteristics of thyroid carcinoma in patients from Qilu Hospital of Shandong University in last 7 years,and to explore the changes in disease spectrum.Methods Records of 772 patients with thyroid carcinoma,who underwent thyroidectomy and pathological examination,were reviewed from January 2005 to December 2011.Results (1) Individuals suffering from thyroid carcinoma have increased rapidly during these 7 years.(2) The high incidence of the disease resided in the age group of 30-59 for both men and women,with a male-to-female ratio of 1 ∶ 2.73.(3) The proportion of papillary microcarcinoma showed a growing trend,the rising trends were also apparent in papillary thyroid cancer (PTC) coexisting with nodular goiter or Hashimoto's thyroiditis (HT).Papillary microcarcinoma accounted for27.27％ of all PTC in 2005 and reached 54.22％ in 2011.Coexistence of PTC and HT accounted for 12.38％ of the cases with a male-to-female ratio of 1 ∶ 6.Conclusion The occurrence of thyroid carcinoma,especially papillary microcarcinoma has been increasing in recent years.The coexistence of PTC with nodular goiter or HT showed a trend to increase.

Objective To explore the role of extracellular signal-regulated kinase (ERK)in central nucleus of amygdala (CeA)in the mechanism of fentanyl-induced hyperalgesia (OIH)in rats. Methods Step 1:12 healthy male Sprague-Dawley rats,weighing 60-100 g,were randomly divided into OIH and Control group.The mechanical paw withdrawal threshold (PWT)and the thermal paw withdrawal latency (PWL)were tested at pre-and post-OIH induction.Then the level of p-ERK in the CeA was analyzed by Western blotting.Step 2:After successful induction of OIH and catheterization in CeA,another 30 SD male rats were randomly divided into 5 groups (n = 6 each):Group OIH;Group OIH+U0124;Group OIH+U0126(0.1 5 nmol);Group OIH+U0126(0.45 nmol)and Group OIH+U0126 (1.5 nmol),then 0.3 μl of DMSO,U0124 (1.5 nmol),U0126 (0.1 5 nmol,0.45 nmol,1.5 nmol)was given through the catheter separately.PWT and PWL were tested before cathe-terization,at pre-OIH induction,post-OIH induction and 0.5 h after CeA drug administration.After the last test of pain threshold,the rats were sacrificed and CeA tissues were sampled for analyzing the expression of p-ERK by western blot.Results In step 1 compared with control group,PWT and PWL of OIH group were sharply decreased post-OIH induction (P <0.05),concomitant increase of the expression of p-ERK in CeA in OIH group was also observed.In step 2,both PWT and PWL were sharply decreased post-OIH induction (P <0.05).Intra-CeA U0126 injection,but not U0124, reversed both behavioral hyperalgesia and molecular activation of ERK in CeA in a dose-dependent manner (P <0.05).Conclusion ERK plays a pivotal role in the maintenance of fentanyl-induced hy-peralgesia.Targeting inhibition of ERK activation in CeA can alleviate fentanyl-induced hyperalgesia.

Objective To compare the clinical effect of povidone-iedine cream plus vagina scrubbing with traditional preoperative vaginal preparation method in order to seek a more safe,effective,convenient and efficient method to prepare the vagina.Methods 100 cases of married patients undergoing elective gynecological surgery were divided into the observation group and the control group with 50 patients in each.The observation group adopted povidone-iodine cream plus vagina scrubbing and the control group used traditional method.Two groups were prepared in the vagina before and after taking cervical swabs for bacterial culture to compare the positive rate of bacterial culture.Results Intra-group comparison of vaginal bacterial culture before and after vaginal preparation was significantly different,but no statistical significance was seen between the two groups.Conclusions Vagina preparation with povidoneiodine cream plus vagina scrubbing can not only reduce the workload of nurses but also improve patient comfort degree.

Objective To compare effect of chemotherapy agent DDP to MACS in sorting cancer stemcells (CSC) of laryngeal carcinoma cell line Hep-2. Methods CD133 magnetic beads were applied to sort Hep-2 cells. Different dosages of DDP were used to treat Hep-2 cells for 48 hours. Enrichment rate of CD133+ cells by MACS and after DDP treatment was detected by Flow Cytometer (FCM). Morphologic change was observed under inverse-phase microscope. Results FCM showed that the sorting rate of CD133+ cells through MACS was 64.33 %, while after DDP treatment for 48 hours, the rate of CD133+ cells was enriched significantly in each dosage of DDP, with the maximal rate was 50.7 %, in the dosage of 4 μg/ml. There was a significantly difference between MACS and each of DDP group (P <0.01). Cells treated with DDP were abnormal in morphology. Conclusion MACS and DDP sorting has respective advantages in enriching CSC in Hep-2 cell lines.

Objective To develop measles and Japanese encephalitis(JE)combined attenuated live vaccine. Methods The independent development of China′s Shanghai 191 measles vaccine strains and Japanese encephalitis SA14-14-2 vaccine strains were selected to prepare two of monovalent vaccines solution,which were used to conduct the interference test,the ratio test,the screening test of protection agent,the screening test of dilution,and the alternative of the curve of freeze-dried.Results The preparation technology of measles and Japanese encephalitis combined live attenuated vaccine was determined.The batches of the products met the quality requirement of the three kinds of monovalent vaccine in the Pharmacopoeia of the people's Republic of China(edition 2005,volume 3).The cross-interference and increased response were not found in the animal test,and the immune response of mouse was not influenced,which indicated the safety and immunogenicity of with good combined vaccine.Conclusion Measles and Japanese encephalitis combined attenuated live vaccine was developed successfully.

Objective To evaluate the effect of laparoscopic myomectomy. Methods Clinical data of 62 cases of laparoscopic myomectomy were analyzed retrospectively. Results Success was achieved in all the operations.Of the 62 cases,intramural myoma was confirmed in 45 cases,and subserous myoma,17 cases (non-pedicled,10 cases; pedicled,7 cases).A total of 101 myomas were removed,with a diameter of 1～9 cm (mean,6 8 cm).The mean operative time was 25～270 min (mean,99 min),and the intraoperative blood loss,10～400 ml (mean,63 ml).The postoperative hospital stay was 2～8 days (mean,4 7 days) and the overall complication rate was 25 8% (16/62).Follow-up observation in 42 cases for 2～36 months (mean,9 months) found recurrence in 2 cases and full-term pregnancy in 2 out of 10 cases with infertility. Conclusions Laparoscopic myomectomy has advantages of safety,quick recovery,short hospital stay and fewer complications.

Surgery is the first choice for the treatment of early non-small cell lung cancer(NSCLC),and lymph node metastasis is the most important factor influencing prognosis of resectable NSCLC.A large number of studies have identified the importance of systematic mediastinal lymph node(N2) dissection,which is also an important basis for the development of NCCN guidelines.But there are few reports about the dissection of intrapulmonary lymph node(N1),especially no detailed study on the dissection of the lymph nodes around the bronchi of non-primary tumor-bearing lobes and segments in lung cancer,and the NCCN guidelines were not detailed elaborate.This article will make a summary of the research on the correlation of intrapulmonary lymph node(N1) dissection.

In order to evaluate the effect of chinese medicine (Yi Qi Xiao Zheng decoction) in reducing radiation side effect, an observation of peripheral blood WBC, stem cell amount, the function of liver and renal in mice was made during and after radiation.The results showed that WBC and stem cell amount were higher in Chinese medicine combined with radiation group than radiation only group after 3 fraction radiation(P