1.Effect of Anchang Decoction on intestinal flora and fecal calcitonin in rats with severe ulcerative colitis
Xuan YU ; Sihua LAI ; Dan GAO ; Zhiren ZHU ; Zebin TANG ; Linguang SI ; Pinghao NONG ; Pingliang SUN
International Journal of Traditional Chinese Medicine 2023;45(6):714-718
Objective:To investigate the effects of Anchang Decoction on intestinal microflora and faecal calprotectin (FC) in rats with severe ulcerative colitis based on probiotics.Methods:Totally 50 rats were taken to prepare a model of severe ulcerative colitis. The rats who successfully modeled were divided into model group, Anchang Decoction low-, medium-, high-dosage groups, Lizhu Changle group, and mesalazine group, with 5 rats in each group. Another 6 rats were set and the blank group. Lizhu Changle group received Lizhu Changle suspension (containing 50 million live bacteria/ml) for gavage; Anchang Decoction low-, medium-, high-dosage groups received Anchang Decoction 1, 5, 10 ml/(kg·d) for gavage; Mesalazine group received Mesalazine suspension (10.5 mg/100 g) for gavage. Rats in the blank group and model group were gavaged with the same volume of normal saline for 7 days. Body mass and disease activity index (DAI) before and after administration were measured. 16s rDNA of lactobacillus and bifidobacterium in feces of rats was detected. FC content of rats was detected by ELISA.Results:Compared with the model group, the weight of rats in each administration group increased ( P<0.05), DAI score decreased ( P<0.05), and the level of calprotectin in feces decreased ( P<0.05), and there was no significant difference in the levels of lactobacillus and bifidobacterium in each administration group ( P>0.05). Conclusion:Anchang Decoction can improve the intestinal micro-ecology by regulating the level of calprotectin in rats with severe ulcerative colitis, thereby playing a therapeutic role.
2.Effects of Anchang Decoction on TLR 4/NF-κB Signaling Pathway and the Expression of Fecal Calprotectin in Rats with Ulcerative Colitis Induced by TNBS
Yunte LIANG ; Zhonghai HUANG ; Zhiyuan LIAO ; Qin ZHANG ; Yong TANG ; Pingliang SUN
China Pharmacy 2021;32(2):189-194
OBJECTIVE:To st udy the effects of Anchang decoction on TLR 4/NF-κB signaling pathway and the expression of fecal calprotectin (FC)in TNBS-induced ulcerative colitis (UC)model rats . METHODS :SD rats were randomly divided into blank group ,model group ,positive control group [Live Bifidobacterium capsules ,5 mL(containing Bifidobacterium 0.35 g)], Anchang decoction low -dose,medium-dose and high-dose groups (1,5,10 mL,each milliliter is approximately equivalent to 0.11 g of total crude drug ),with 15 rats in each group. Other groups were given TNBS combined with ethanol enema to establish UC model rat ,except blank group was given normal saline. Two days after successful modeling ,blank group and model group were given normal saline 5 mL,administration groups were given relevant medicine intragastrically ,once a day ,for consecutive 14 d. After last medication ,HE staining was used to observe the pathological change of colon tissue in rats. Western blotting assay was used to detect the protein expression of TLR 4,TRAF6 and NF-κB in colon tissues of rats;Real-time fluorescent quantitative PCR was used to detect mRNA expression of TLR 4,TRAF6,TNF-α and NF-κB;ELISA assay was adopted to detect serum level of TNF-α,IL-6 and FC in stool samples. RESULTS :Compared with blank group ,the colonic mucosa of model group was severely damaged,and the protein expression of TLR 4,TRAF6 and NF-κB,mRNA expression of TLR 4,TRAF6,TNF-α and NF-κb as well as serum levels of TNF-α,IL-6 and FC level in stool samples were increased significantly (P<0.05). Compared with model group,the pathological changes of colon tissue in rats were improved in different administration groups to different extents ,and above indexes were all decreased significantly (P<0.05). CONCLUSIONS :Anchang decoction may relieve the inflammation of UC model rats by regulating the TLR 4/NF-κB signaling pathway and the expression of FC.
3. Clinical analysis of 31 cases of 2019 novel coronavirus infection in children from six provinces (autonomous region) of northern China
Duan WANG ; Xiuli JU ; Feng XIE ; Yan LU ; Feiyu LI ; Huihong HUANG ; Xiuling FANG ; Yuanjun LI ; Jianyun WANG ; Bin YI ; Juxia YUE ; Jing WANG ; Lingxiao WANG ; Bo LI ; Yi WANG ; Bingping QIU ; Zhiyuan ZHOU ; Keliang LI ; Jianhua SUN ; Xuegong LIU ; Guodong LI ; Yongjun WANG ; Aihua CAO ; Yanni CHEN
Chinese Journal of Pediatrics 2020;58(4):E011-E011
Objective:
To analyze the epidemiological history, clinical manifestations, treatment and the short-term prognosis of 31 cases of 2019 novel coronavirus(2019-nCoV) infection in children from six provinces (autonomous region) in northern China.
Methods:
A retrospective analysis of the epidemiological history, clinical symptoms, signs, laboratory examinations, chest imaging, treatment and the short-term prognosis of 31 cases of 2019-nCoV was conducted. The patients were diagnosed between January 25th, 2020 and February 21st, 2020 in 21 hospitals in 17 cities of six provinces(autonomous region) of Shaanxi, Gansu, Ningxia, Hebei, Henan and Shandong.
Results:
The age of the 31 children with 2019-nCoV infection was 7 years and 1 month (6 months -17 years). Nine cases (29%) were imported cases. Other 21 cases (68%) had contact with confirmed infected adults. One case (3%) had contact with asymptomatic returnees from Wuhan. Among the 31 children, 28 patients (90%) were family cluster cases. The clinical types were asymptomatic type in 4 cases (13%), mild type in 13 cases (42%), and common type in 14 cases (45%). No severe or critical type existed. The most common symptom was fever (