Objective　To investigate the involvement of cell cycle negative regulator p16 in the replicative senescence of normal human diploid fibroblasts. 　Methods　 p16 cDNA and retroviral vector was introduced into normal human fibroblast 2BS cells by transfection technique. Then the effects of p16 on replicative cellular senescence of 2BS cells were examined. 　Results　 Compared with the control cells, 2BS cells transfected with p16 cDNA showed significant suppression of growth rate (decrease 50%) with cell cycle arrested at G1 phase. This phenomenon similar to that of the senescent cells, with the cellular response to stimulation by growth factor decreased 79.4%, showing the characteristics in morphology of senescent fibroblasts. 　Conclusions　 The over expression of p16 gene contributes to the process of cellular senescence of normal human diploid fibroblasts.

Adult ; Aged ; Bone Screws ; Calcaneus ; injuries ; surgery ; Female ; Fracture Fixation, Internal ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged

Adolescent ; Adult ; Bone Marrow Transplantation ; immunology ; Bone Morphogenetic Proteins ; therapeutic use ; Bone Neoplasms ; therapy ; Combined Modality Therapy ; Extremities ; pathology ; Female ; Humans ; Male ; Middle Aged ; Neoplasms ; therapy ; Transplantation, Autologous ; immunology ; Young Adult

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bone Nails ; Female ; Fracture Fixation, Internal ; Fractures, Bone ; diagnostic imaging ; physiopathology ; surgery ; Humans ; Male ; Middle Aged ; Patella ; injuries ; Tomography, X-Ray Computed ; Young Adult

OBJECTIVETo explore factors influencing acetabular development after closed reduction in developmental dislocation of the hip (DDH), to provide theoretical basis for improving the therapeutic effects of closed reduction treatment of DDH.

METHODSA total of 100 children with single side DDH who were treated by closed reduction from January 2002 to December 2005 were followed up, and the AI and AI (D/W) of dislocation hip were measured at the 12th month after reduction. Ten factors such as gender, side, age, AI and AI (D/W) of dislocation hip before reduction, degree of dislocation, the rate of width of femoral head, femoral neck anteversion of dislocation hip before reduction, h/b rate,cut off adductor and skeletal traction et al were taken as independent variable, AI and AI (D/W) at the 12th month after reduction were taken as dependent variable,then multiple linear stepwise regression analysis was used to screen the major influencing factors.

RESULTSAge, gender, degree of dislocation, h/b rate, the rate of width of femoral head and femoral neck anteversion exerted obviously influence on AI and AI (D/W),and then age,degree of dislocation and FNA had positive correlation with AI and negative correlation with AI (D/W); the rate of width of femoral head and h/b rate had negative correlation with AI and positive correlation with AI(D/W). The development of acetabular of female patients was faster than that of male patients.

CONCLUSIONAge, gender,degree of dislocation, concentric reduction or not, the development degree of femoral head before reduction and proximal femoral shape are the major factors influencing acetabular development.

Acetabulum ; abnormalities ; growth & development ; surgery ; Age Factors ; Child, Preschool ; Female ; Hip Dislocation ; surgery ; Humans ; Infant ; Male ; Sex Factors ; Treatment Outcome

Objective: To prepare the monoclonal antibody against ankyrin repeat domain 22（ANKRD22）and to investigate its expression in colorectal cancer tissues. Methods : The recombinant human ANKRD22 was expressed through E. coli and pET-42a and then used to immunize Balb/c mice after purification. Anti-human ANKRD22 specific monoclonal antibodies were selected by Western blotting with 293T cell lysate highly expressing ANKRD22 as antigen. The expression of ANKRD22 in the tissue microarrays of 112 patients with colorectal cancer was detected by immunohistochemical staining. Results : Four specific monoclonal antibodies against human ANKRD22 were screened out of 93 hybridoma cells,which reacted well with natural human ANKRD22. ANKRD22 was mainly distributed in the cytoplasm of colorectal cancer cells. In 112 cases of colorectal cancer,94 cases were detected positive for ANKRD22 expression,with the positive rate of 83.93%. The expression of ANKRD22 was statistically correlated with the expression of p53 and β-catenin（P<0.05）,but not with age,sex,location of tumors,AJCC stage,Dukes stage,degree of differentiation,lymph node metastasis and mismatch repair gene expression（P>0.05）. Conclusion The expression level of ANKRD22 was high in colorectal cancer. ANKRD22 might be involved in the carcinogenesis of colorectal epithelium and be a potential diagnostic marker.

OBJECTIVETo observe the clinical therapeutic effect of treatment for long bone fracture nonunion in limbs by bone morphogenetic protein compounds combined with autologous red bone marrow graft.

METHODSFrom January 2004 to December 2010,36 cases of long bone fracture nonunion in limbs were treated by bone morphogenetic protein compounds combined with autologous red bone marrow graft. There were 22 males and 14 females with an average age of 36.8 years old ranging from 22 to 68 years. Nonunion sites included humerus in 6 cases,ulna in 4 cases,radius in 3 cases,femur in 10 cases,and tibia in 13 cases. The latest reconstruction was performed on these 36 cases during 8 to 24 months (means 13.7 months) after injuries. There were 22 cases of hypertrophic nonunion,14 cases of atrophic nonunion. Main clinical symptoms were pain of fracture sites on weight bearing: and swelling of limbs. Clearness of fracture line and bone defect were indicated on X-ray. The therapeutic effect of the treatment after operation was been evaluated by wound healing, reaction in the area of bone graft and bone marrow aspiration,fracture healing,and recovery of adjacent joint function.

RESULTSAll incisions primarily healed,immunologic rejection and anaphylaxis were not detected in these incisions. Infection and haematoma formation were not detected in the area of bone marrow aspiration. All these 36 cases were followed up for 3 to 20 months (means 16.2 months). Chronic pain in the area of bone marrow aspiration, bone infection in the area of bone graft, and red swelling of the skin near incisions or sinus tract were not detected. Bony union was achieved in all cases in 3 to 12 months (means 6.2 months) after operation, malunion was not detected. Pain of fracture sites on weight bearing and swelling of limbs disappeared after bony union. The adjacent joint function completely recovered in most cases,only 5 cases remained some function limited part.

CONCLUSIONIn the treatment of long bone fracture nonunion in limbs, bone morphogenetic protein compounds combined with autologous red bone marrow graft have the advantage of wide range of sources, safety and promoting bone union,which is one of the ideal bone graft

Adult ; Aged ; Bone Marrow Transplantation ; Bone Morphogenetic Proteins ; urine ; Extremities ; injuries ; Female ; Fracture Healing ; Fractures, Ununited ; therapy ; Humans ; Male ; Middle Aged ; Transplantation, Autologous

OBJECTIVETo study technique and clinical therapeutic effects of internal fixation with three-column plates for the treatment of complex tibial plateau fractures through antero-midline and postero-medial approaches.

METHODSFrom January 2010 to December 2012, 28 patients with complex tibial plateau fractures were treated with internal fixation using three-column plates through antero-midline and postero-medial approaches. There were 17 males and 11 females, with an average age of 45.3 years old (ranged, 28 to 64 years old). Twelve patients had injuries in the left side and 16 patients had injuries in the right side. According to Schatzker classification, 12 patients were type V, 16 patients were type VI. According to three-column classification, all the patients had injuries of lateral, medial and posterior columns. The mean interval from injury to operation was 9.4 days (ranged, 6 to 15 days). The main clinical symptoms were knee joint swelling, deformity and limitation of motion before operation. The X-ray and CT showed all patients had complex tibial plateau fractures, which involved in the lateral, medial and posterior columns. The therapeutic effects were evaluated by fracture healing time, hospital for special surgery knee score (HSS) at one year after operation. The indexes such as tibial plateau-tibial shaft angle (TPA), posterior slope angle (PA) and femoral-tibial angle (FfA) were compared between immediate postoperation and one year after operation.

RESULTSAll incisions primarily healed without postoperative complications such as infection and cutaneous necrosis. All the patients were followed up, and the duration ranged from 12 to 24 months, with a mean of 18.1 months. The bone union time ranged from 5 to 10 months (mean, 7.8 months) after operation. Knee joint swelling and pain disappeared after bony union, and joint function completely recovered. The results of hospital for special surgery knee score (HSS) was 27.81 ± 2.17 in pain, 19.52 ± 2.05 in function,15.82 ± 1.73 in passive range of motion, 8.51 ± 1.32 in muscle strength, 8.33 ± 1.08 in flexion deformity, 9.36 ± 0.52 in joint stability, and the total mean score was 89.35 ± 3.19. According to results of HSS, 20 patients got an excellent result, 5 good,2 fair and 1 poor. There were no significant differences in tibial plateau-tibial shaft angle (TPA), posterior slope angle (PA) and femoral-tibial angle (FTA) between immediate postoperation and one year after operation.

CONCLUSIONThree-column plate internal fixation for the treatment of complex tibial plateau fractures through antero-midline and posteromedial approaches is effective to achieve anatomic reduction,rigid internal fixation and early functional exercise.

Adult ; Bone Plates ; Female ; Follow-Up Studies ; Fracture Fixation, Internal ; methods ; Humans ; Male ; Middle Aged ; Tibial Fractures ; physiopathology ; surgery

The diagnosis of bone metastasis of prostate cancer (PC) is of great significance to the treatment and prognosis of patients with PC.Bone scan is the most commonly used in the early diagnosis of bone metastasis, but its specificity is low and there is a high false positive.In recent years, with the in-depth study of the application of CT, MRI, emission computed tomography (ECT), positron emission computed tomography/computed tomography (PET/CT) and deep learning algorithm-convolutional neural networks (CNN) in the diagnosis of bone metastasis, the combined application of various auxiliary parameters in the diagnosis of bone metastasis has significantly been improved. The therapeutic effect of PC patients with bone metastasis can also be evaluated, which is expected to achieve the treatment of bone metastasis as well as diagnosis. By systematically expounding the research progress of the above-mentioned techniques in the diagnosis of bone metastasis, it can provide clinicians with new methods for the diagnosis of bone metastasis and improve the diagnostic efficiency for bone metastasis.
Bone Marrow Diseases ; Bone Neoplasms/diagnostic imaging* ; Humans ; Magnetic Resonance Imaging ; Male ; Positron Emission Tomography Computed Tomography ; Prostatic Neoplasms/diagnostic imaging*