1.Genetic Manipulation on Biosynthesis of Terpenoids
China Biotechnology 2006;0(01):-
Terpenoids constitute a chemically diverse family of naturally occuring compounds with pharmaceutical significance, such as artemisinin and taxin. They are synthesized in many kinds of microorganisms and plants with very low yield. Metabolic engineering, modifying metabolic pathways of terpenoids-synthesized cells by recombinant DNA techniques, has been developed and applied to improve the production of terpenoids, as well as create a novel biosynthesis pathway of terpenoids in terpenoid-free organisms. The biosynthetic pathway of terpenoids was sumed up, and recent progress of metabolic engineering aiming at raising content of terpenoids in microorganisms and plants was described.
3.Investigation and analysis of critical thinking ability in medical students
Ya-qing, ZHANG ; Li-sha, LI ; Ping, WU ; Ying, CHEN
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(7):869-872
Objective To investigate the critical thinking ability of medical students and its related factors in order to provide evidence for higher medical education reform. Methods A total of 325 medical students were selected by cluster sampling method, and Critical Thinking Disposition Inventory-Chinese Version (CTDI-CV) was adopted for survery. Results The average total score of critical thinking ability of medical students was above 280, and 51.08% of students showed positive critical thinking ability. Among all the seven critical thinking dispositions, average scores of each item were all positive (>40) except that the significance of truth seeking (37.23) and systematicity (39.05) were indefinite. There were significant differences in critical thinking ability between medical students with different gender and academic years (P<0.05). Conclusion The critical thinking ability of medical students is positive, however, there still exist some shortages. An equal relationship between teachers and students should be established, the traditional course system be continuously reformed, a good environment for clinical training be created, and a variety of teaching methods be implemented to further strengthen the critical thinking ability of medical students.
4.Consistency study of PowerPlex 21 kit and Goldeneye 20A kit and forensic application.
He REN ; Ying LIU ; Qing-Xia ZHANG ; Zhang-Ping JIAO
Journal of Forensic Medicine 2014;30(3):191-193
UNLABELLED:
To ensure the consistency of genotype results for PowerPlex 21 kit and Goldeneye 20A kit.
METHODS:
The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained.
RESULTS:
All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference (P > 0.05). The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymorphism information content was 0.810.
CONCLUSION
PowerPlex 21 kit and Goldeneye 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.
DNA/analysis*
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DNA Fingerprinting
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DNA Primers
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Genotype
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Heterozygote
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Humans
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Microsatellite Repeats
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Paternity
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Polymerase Chain Reaction
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Polymorphism, Genetic
5.Comparision of non-fasting with fasting blood lipid testing in in-hospital patients
Ying GAO ; Yuanlin GUO ; Naqiong WU ; Chenggang ZHU ; Ping QING ; Geng LIU ; Qian DONG ; Jianjun LI
Chinese Journal of Laboratory Medicine 2017;40(6):431-435
Objective To explore the feasibility of the application of non fasting blood lipid in the hospitalized population.Methods Self-control study was used.608 patients(aged 20~86 years old) were enrolled from April 2015 to October 2016 in lipid center of FuWai hospital.Fasting sample and non-fasting sample(1~4 h after breakfast) were collected from every patient and lipid profile including TG (triglyceride), TC (total cholesterol), HDL-C (high density lipoprotein cholesterol) and LDL-C (low density lipoprotein cholesterol) were measured in clinical laboratory.The results of two tests were compared using the Wilcoxon signed-rank test.Results The differences between non-fasting and fasting lipid test were +0.47 mmol/l (+30%) for TG,-0.03 mmol/l (-2.8%) for HDL-C,-0.09 mmol/l (-3%) for LDL-C and-0.24 mmol/l (-8.7%) for calculated LDL-C (P<0.001 respectively).The differenceswere +0.01 mmol/l for TC and +0.02 mmol/l for non-HDL-C,therefore no statistical difference was observed.When the TG level was stratified,the level of non-fasting LDL-C using directing test method was not significantly different between TG> 4.5 mmol/L and the whole (0.07 vs.0.09),but the level of non-fasting LDL-C using formula method wassignificantly different between TG> 4.5 mmol/L and the whole (0.66 Vs.0.24),andthe drops were 34.9% vs.8.7%.Conclusion Non-fasting lipid test could be an effective routine method for lipid evaluation in the hospitalized population.
7.Relationship between polymorphism of Aiolos exon 8+488C/T and bronchial asthma
ya-ping, YUAN ; min, ZHOU ; qing-yun, LI ; huan-ying, WAN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(01):-
0.05). Conclusion The exon 8+488C/T polymorphism of Aiolos gene exists in this population of Han ethnics,however,it is not associated with bronchial asthma.
8.Effects of low-intensity laser irradiation on aortic atherosclerosis in rabbits
Shi-Quan XU ; Jian-Zhong ZHANG ; Ying-Qi LIU ; Qing-Ping JING ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(07):-
Objective To observe the effects of low-intensity(650 nm,5 mW)laser irradiation on the pathological changes associated with aortic atherosclerosis using a rabbit model.Methods Thirty-six male Zelanian rabbits were randomly divided into 4 equal groups:a control group,a laser irradiation group,a simvastatin treatment group and a laser plus simvastatin group,and were treated accordingly.After being fed the basic diet for a week,all the animals were fed a high fat diet during the experiment.Blood samples were taken for lipid assay at the 60th day. The animals were sacrificed at the 61st day and their aortas removed for gross and microscopic examination.Any pathological changes were graded as mild,intermediate or severe according to the indicators of atherosclerosis observed.Results There were no significant differences in blood fat levels among the four groups before the experi- ment,but significant differences in serum triglyceride prevailed after the treatments.Low-density lipoprotein(LDL)- C level in the control group was significantly higher than those in the laser irradiation,simvastatin treatment and laser plus simvastatin groups.There was also a very significant difference in high-density lipoprotein(HDL)-C levels among the laser irradiation group,the laser plus simvastatin group and the control group.The pathological changes observed were correlated with blood fat levels.Mild atherosclerosis was found in the treated groups,but severe or in- termediate atherosclerosis was more prevalent in the control group.Conclusion Low-intensity laser irradiation a- lone or combined with simvastatin can significantly decrease blood lipid levels and the severity of pathological changes associated with aortic atherosclerosis in this animal model.
9.Effect of orthokeratology on progression of juvenile myopia and the relative influencing factors
Jia-Li, LI ; Shu-Yan, LI ; Min-Yu, CHEN ; Yu-Ying, XIAO ; Qing-Yang, LIU ; Ping-Ping, MA
International Eye Science 2017;17(8):1516-1518
AIM: To evaluate the effect of orthokeratology on progression of juvenile myopia and analysis its influencing factors.METHODS: Totally 97 patients (189 eyes,aging from 8 to 17 years old) who received orthokeratology lenses treatment in our hospital from January 2012 to December 2014,were followed up for 2a.The visual acuity,corneal curvature,diopter,and ocular axial length were observed.Factors of influencing myopia progress in juvenile were analyzed.RESULTS: At 1mo after receiving orthokeratology contact lenses,the visual acuity and corneal curvature were changed compared with that of before(P<0.001).After 2a of receiving orthokeratology contact lenses,the difference was significant compared with baseline: spherical equivalence (-0.51±0.64D,t=10.864,P<0.001),axial length(0.33±0.31mm,t=14.879,P<0.001),corneal astigmatism (-0.25±0.43D,t=5.375,P<0.001).Statistic analysis showed that there was a negative correlation between the spherical equivalence and age,baseline of diopter or ocular axial length(P<0.05).CONCLUSION: Orthokeratology can effectively improve the visual acuity of patients.Although there is slightly progression in diopter and ocular length after 2a of wearing orthokeratology contact lenses.Orthokeratology is an effective treatment on controlling progression of juvenile myopia,especially in the elder children who with the longer basic axial length and the greater diopter.
10.Application of Ion Torrent PGM™ System in Detection of Fetal DNA in Maternal Plasma.
Ya-nan LIU ; Xue-ying ZHAO ; Yuan PING ; Qing-wen XU ; Jiang-ping HUANG ; Kai-nan ZOU ; Huai-gu ZHOU
Journal of Forensic Medicine 2015;31(6):432-435
OBJECTIVE:
To explore the feasibility of detecting of Y-STR of fetal DNA in maternal plasma using Ion Torrent PGM™ System.
METHODS:
A total of 16 fetal DNA samples from maternal plasmas (8 cases from 38 weeks gestational age and 8 ones from 12 weeks) were prepared and a multiplex assay with 7 STR loci (DYS390, DYS391, DYS393, DYS438, DYS437, DYS456, DYS635) was designed for multiplex-PCR amplification. Using Ion Torrent PGM™ System, the results of Y-STR sequences and capillary electrophoresis were obtained and compared.
RESULTS:
Y-STR specific alleles were detected in the maternal plasma of all the pregnant women having male babies of second and third trimester, which were higher than that detected by capillary electrophoresis. Consistent Y-STR genotypes were observed between fetal DNA from maternal plasma and genomic DNA from the newborn babies.
CONCLUSION
Based on Ion Torrent PGM™ System, the prenatal Y-STR detection method may provide a high-sensitive and high-throughput choice for prenatal STR detection in forensic testing.
Alleles
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Chromosomes, Human, Y/genetics*
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DNA/blood*
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Family
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Female
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Fetal Blood/chemistry*
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Genotype
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Haplotypes
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Humans
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Male
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Polymerase Chain Reaction
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Polymorphism, Genetic
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Pregnancy
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Sensitivity and Specificity
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Sex Determination Analysis
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Tandem Repeat Sequences/genetics*