Objective To investigate the effect of intensive nursing intervention on the secondary infection in chemotherapy of elderly patients with lung cancer.Methods 168 cases of elderly lung cancer patients whose neutrophil ≤ 1.0×109/L during chemotherapy were randomly divided into two groups,namely,the intensive care group and the control group,with 84 cases in each group.The control group was given routine nursing of infection prevention,while the intensive care group was given intensified nursing for infection prevention.A contrastive analysis was conducted in terms of the incidence,clinical characteristics and the duration of antibiotic treatment of the two groups of patients with secondary infection.Results The secondary infection rate of the intensive care group was lower than that of the control group during chemotherapy.What's more,the duration of fever and treatment of the intensive care group was shorter than that of the control group,hence the patients were safe during infection period.The difference was statistically significant.Conclusions Strengthening the nursing intervention can reduce the incidence rate of secondary infection in aged patients with lung cancer,increase the tolerance of patients to chemotherapy and ensure the successful completion of the chemotherapy cycle,prolong the life span of the patients with an improved quality of life.

Objective To explore the expression level and clinical significance of microRNA‐451 (miR‐451) in the plasma of patients with diffuse large B lymphoma (DLBCL) .Methods We enrolled 27 patients with DLBCL and 27 healthy controls for the study .The plasma level of miR‐451 was detected by reverse transcription polymerase chain reaction (RT‐PCR) .Then we analyzed the relationship between the plasma level of miR‐451 and pathological characteristics of DLBCL .Results The plasma level of miR‐451 in the patients with DLBCL was significantly lower than in healthy controls [(0 .41 ± 0 .15) vs .(0 .64 ± 0 .21)] (P<0 .01) .However ,the plasma level of miR‐451 was not significantly correlated with the patients'gender ,age ,serum LDH level or primary site (P>0 .05) ,but with Ann Arbor tumor stage and IPI score ( P< 0 .05 ) .Conclusion The plasma level of miR‐451 decreases in patients with DLBCL ,which indicates that miR‐451 may participate in the pathology of DLBCL .

Objective:To discuss the value of ultrasonography in the diagnosis and differential diagnosis of inguinal masses in children. Methods:Five hundred and eighty-two patients were examined by ultrasonography,and the sonogram features were observed. Results:The feature of sonogram of majority cases were distinctive,which were can be separated into three kinds as spina bifida cystica,parenchymatous and hyperechogenicity. Conclusion:The ultrasonic examination should be used as the regular differential diagnostic method for inguinal masses in children.

Objective To investigate the CT features of active pulmonary tuberculosis.Methods The clinical data and HRCT in 79 patients with histologically proved active pulmonary tuberculosis were studied retrospectively.The diagnosis of active pulmonary tuberculosis was based on positive acid-fast bacilli in sputum (43 patients) and the features at the serial radiographs obtained treatment period(36 patients).Results The main CT finding of active pulmonary tuberculosis were centrilobular nodules in 65 cases(82.3),tree in bud appearance in 65 cases(82.3),thickening of bronchial wall in 58 cases(73.5), consolidation in 61 cases(77.3), cavity in 56(73.2), fluid-bronchogran in 53cases(72.8),ground-grass sign in 45 cases(56.8),line shadows in 55 cases(73.1),pleural thickening in 21 cases (26.6), hilar and mediastinal adenopathy in 12 cases(15.1) and more two abnormal findings co-existed in 79 cases.The centrilobular nodules (n=25) ,tree in bud appearance (n=26) ,thickening of bronchial wall(n=8), consolidation (n=19) and ground-grass shadows (n=21) were improved after therapy .Conclusion The active pulmonary tuberculosis are of some specific HRCT manifestations,which may be helpful to improve the dignosis combined with other clinical formation.

OBJECTIVE:To observe the clinical efficacy of Xuebijing injection for hemorrhagic fever with renal syndrome(HFRS) complicating acute renal failure(ARF). METHODS:A total of 72 patients with HFRS complicating ARF were randomly assigned to receive combined therapy of western medicines(control group,n=30) or combined therapy of western medicines in combination with Xuebijing injection(trial group,n=42). RESUTLS:There were significant differences between the trial group and the control group in total effective rate (95.24% vs.80.00%) (P

OBJECTIVE:To analyze the utilization and cost of three kinds antibiotics in patients undergoing coronary artery bypass graft (CABG). METHODS:Eight four CABG patients were divided into three groups:Mezlocillin Sodium group (n=28),Cefuroxime Sodium group (n=28) and Ciprofloxacin group (n=28). The postoperative infection rate,changes in body temperature WBC count and the cost of antibiotics were compared among the 3 groups. RESULTS:No postoperative infection case was noted in all the 3 groups. The duration for breathing machine used in Cefuroxime sodium group was the shortest at (14.21?5.45) hours,and the average cost for antibiotics in this group was also the lowest at (678.28?256.74) yuan,showing significance as compared with the other two groups (P

Cytokines of the transforming growth factor (TGF-?)family exert multiple biological effects by their signal transduction pathways that undergo a basic course of ligands of TGF-? family→receptors→SMADs→transcription factors→DNA expression. The genetic variety of different signal molecules in transduction pathways of TGF-? family were found in various benign or malignant tumors, and this had much significance in designing effective therapeutic strategy.

0.05). Conclusion:Accurate working length may be obtained with RootZX electronic apex locator.

Objective:To investigate the mechanism of ~(131)I-GM-CSF inducing apoptosis of HL-60/ADM cells.Methods:An in vitro radioimmunotherapy(RAIT) model was employed.Data were collected from transmission electrion microscopy,flow cytometric analysis and immunocytochemistry assay.Results:After exposure to ~(131)I-GM-CSF,HL-60/ADM cells were induced to apoptosis.The cell mitochondrial transmembrane potential(MTP) reduced and HL-60/ADM cells appeared the classical apoptotic morphology.The Bax was up regulated,while Bcl-2 and Bcl-xl was decreased.Conclusion:~(131)I-GM-CSF can induce HL-60/ADM apoptosis through opening the mitochondrial permeability transition pore and reducing MTP,which suggests that ~(131)I-GM-CSF may be available in therapy of AML.

Objective To examine the effectiveness of a gene transfer of rat glial cell-line derived neurotrophic factor(GDNF)into Schwann cells Methods Rat GDNFcDNA was amplified from newly-born rat sciatic nerve by RT-PCR and ligated into the retroviral vector pLNCX-2 A fter being packaged by the amphotropic packaging cell line PT67,the viral supernatants from these anti-clonal producing lines were titred on fibroblast N IH3T3 The highest tire recombinant retrovirus was used to infect deviding populations of newly-born rat SCs The level of GDNF mRNA in GDNF-SCs and normal SCs was tested by RT-PCR The GDNF protein in genetically modified SCs and normal SCs was assayed by immunocytochemistry The amounts of GDNF in conditioned medium of GDNF-SCs in different phrase and normal SCs were detected by enzyme-linked immunoassay sensitive assay Biological activity of the secreted GDNF was tested using motoneuron bioassay by MTT method Results (1)The GDNF cDNA band of GDNF-SCs was more prominent than that of SCs (2)Cells in both groups were immunohistochemically positive for GDNF expression Staining for GDNF was more prominent in the Scs infected with pLNCX2-GDNF (3)The rate of GDNF secreted by GDNF-Scs was 5 1-fold compared with normal SCs (4)The bioassay comfirmed that the secreted GDNF from GDNF-Scs was biologically active,and more motoneurons survived than normal SCs group Conclusion With the help of retrovirus,GDNF gene can be transferred and stably expressed in SCs,and it′s may be a better way to graft SCs promoing regeneration of PNS injuries.