OBJECTIVE:To establish a method for content determination of baicalin in Scutellaria baicalensis Georgi extract and to optimize the extraction technique.METHODS:The content of baicalin was adopted as index and was deter?mined by HPLC;Single factor test and orthogonal test were employed to optimize the extraction technique.RESULTS:The detected concentration of baicalin showed a good linear relation in the range of0.05912～0.02956mg/ml(r=0.99974),the average recovery was99.5%,RSD=1.10%(n=5);The optimal technique was twice recirculation in water solvent14times the amount of Scutellaria baicalensis Georgi flour,each time for1hour.CONCLUSION:The established method is conve?nient,and the extraction technique is stable,reasonable and practicable.

Objective To discuss the selection of operation methods of total hysterectomy in obese women. Methods We reviewed clinical data of 116 obese women with the body mass index (BMI) ≥ 28 kg/m~2 treated with total hysterectomy. According to the operative procedure, 56 women underwent the vaginal total hysterectomy (Vaginal Group) and 60 women, abdominal total hysterectomy (Abdominal Group). Intra- and post-operative records were compared between the two groups. Results As compared with the Abdominal Group, the Vaginal Group presented a significantly shorter operation time (t=-3.693,P=0.000) and hospital stay (t=-7.019, P=0.000), a significantly less intraoperative blood loss (t=-4.006,P=0.000), and a significantly lower postoperative pyrexia rate (?~2=4.388,P=0.036) and complication rate (?~2=5.243,P=0.022). Conclusions Vaginal hysterectomy is superior to abdominal hysterectomy in obese women, but should be used with caution in those with severe adhesions or multiple abdominal operation history.

The pharmacokinetics of ketamine were studied in 8 preschool children,aged 3.5～6 years. The ketamine was administrated by I. V. infusion at a dose of 3mg?kg~(-1) in one hour constantly. The plasma concentrations of ketamine were measured by high performance liquid chromatography. The disposition of intravenous infusion with ketamine could be described by two-compartment open model, and the variables of pharmacokinetics of ketamine were as follows: T_(1/2?)=0.15?0.04 hours,T_(1/2?)=2.84?0.44 hours,Cl=0.34?0.06L?kg~(-1)?h~(-1), AUC=9.21?1.00mg/L?h, and V_d=0.66?0.29 L?kg~(-1). This suggests that intravenous infusion of ketamine at 3mg?kg~(-1)?h~(-1) is safe for the maintenance of general anesthesia in preschool children, attention must he paid to the children anesthetized with long period of ketamine infusion because recovery may be delayed due to the prolonged elimination half-life.

Objective: To determine the influence of ?-hydroxybutyric sodium (7-OH)on the pharmacokinetics of ketamine infusion in preschool children. Method: Sixteen patients were randomly assigned to group Ⅱ (Ketamine) and group Ⅱ (Ketamine plus ?-OH). After trachea intubation,0.05% ketamine was infused in both groups at 3mg?kg~(-1)?h~(-1) and ?-OH 125mg?kg~(-1) only in group Ⅱ. The plasma concentration of ketamine was measured using high performance liquid chromatography. Pharmacokinetic parameters were calculated using 3P_(87) software composed by Chinese Pharmacologic Society. Result: In both groups,pharmaeokinetics could be descrided as two-compartment open model. The T_(1/2)? and the Cl differ between group Ⅰ and group Ⅱ (P

Objective: To study the expressions of CD147and MMP9 in the lung metastasis model of breast cancer in BCML-TAII99 mice and their correlation with tumor growth and metastasis thereof. Methods: The breast cancer model with lung metastasis was established in BCML-TAII99 mice. The immunohistochemical staining method(SP method) was used to analyze the expressions of CD147 and MMP9 in mice lung metastasis tissue. The relationship between the tumor growth and the metastasis was analyzed. Results: The positive rates of CD147 and MMP9 were 63.33%（19/30）and 53.33%（16/30）respectively in mouse models. There was significant difference in positive rate of expressions between the metastasis group and the non-metastasis group（χ~2= 6.238， P ＝ 0．013； χ~2＝ ＝5．129， P ＝ 0．024). There was a statistically significant positive correlation between the expression of CD147 and MMP9（r = 0．786， P ＝ 0.025). Conclusion: The expression of CD147 may play a crucial role in infiltration and metastasis of breast cancer by inducing the expression of MMP9.

Humans ; Orthodontic Appliances ; Orthotic Devices ; Sleep Apnea, Obstructive ; therapy

Animals ; Chick Embryo ; physiology ; Electrocardiography ; methods ; Heart ; embryology ; physiology ; Heart Rate ; physiology

Cardiology ; Humans ; Myocardial Ischemia ; Physicians ; Stroke

Objective To investigate the expressions of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2) and relationships of these expressions with the clinicopathology in breast cancer patients aged 70 years old and over. Methods The expressions of ER, PR and HER-2 were detected by immunohistochemical staining from 124 elderly breast cancer patients, and the relationships of these expressions with pathological classification, tumor size, lymph node metastasis and TNM stage were analyzed. Results Among 124 patients, the positive expression rate of ER and PR was 75.0% (93/124) and 71.0% (88/124),respectively. They were not associated with pathological classification, tumor size, lymph node metastasis or TNM stage (all P＞0. 05). The positive expression rate of Her-2(+++) and Her-2 (++) was 11.3% (14/124) and 24.2% (30/124), respectively. And the expression of Her-2 was significantly associated with tumor size, lymph node metastasis and TNM stage (P＜0. 05) but not with pathological classification (P＞0. 05). Conclusions The positive expressions of ER and PR are not correlated with the clinicopathology in elderly breast cancer patients. Her-2 overexpression is positively associated with tumor size, lymph node metastasis and TNM stage.

Objective This research is to observe the treatment effects of hip bath and oxygen blowing on severe neonatal diaper rash.Methods 289 neonates with severe diaper rash were randomly divided into three groups.91 cases in the flat tube group,102 cases in the round tube group and 96 cases in the control group.Three groups of neonates were cleaned on the hips and perineuma after poops and then dried with wet tissues and Mupirocin Ointment.The control group was treated with the above-mentioned method.The oxygen blowing groups were treated with hip bath of 1:5000 chameleon solution twice a day.Blowing the hips with oxygen five minutes every time after hip bath.The method of blowing oxygen was that oxygen humidifying containers as normal oxygen aspiration facilities was not filled with water,whose oxygen flowing volume standed at 10 L/min and whose tube blew at the afflicted parts until being dry.The oxygen blower held the oxygen exit and blowed at the afflicted parts in the round tube group and the oxygen blower flattened the oxygen exit and blew at the afflicted parts in the fiat tube group.The treatment effects will be compared among the three groups four days later.Results The cure period of the round tube group was obviously shorter than that of the control group,and the cure period of the fiat tube group was remarkably shorter than that of the round tube group.The total effective rate in the round tube group was obviously higher than that of the control group and the total effective rate in the fiat tube group was obviously higher than that in the round tube group.The difference had a statistical significance.Conclusions The treatment effects for the severe neonatal diaper rash with hip bath and oxygen blowing are remarkable and the oxygen blowing effects with fiat tubes are better than those with round tubes.