Objective To study the protective effect of verapamil against ischemia reperfusion injury when liver was resected.Methods The routine Pringle's maneuver hepatic vascular occlusion in 18～20min. Thirty-nine patients for resection of liver with cirrhosis were randomly divided into two groups, Verapamil group (n=20), the 20 patients with hepatectomy were given by 5mg verapamil through right gastroepiploic vein before blocking porta hepatis.Physiologic saline group (n=19), were injected by 20ml saline in the same way.The variation of postoperative liver function were measured.Liver intracellular [Ca 2+ ]i and adenosine triphosphate (ATP) after being reperfused for 1h were measured as well as the hepatic histopathologic alterations were also examined.Results The levels of intracellular [Ca 2+ ]i concentrations,alanine aminotransferase (ALT) and aspartate aminotransferase(AST)from the verapamil group increment less than those of the physiologic saline group(P

Objective To investigate the status of Ureaplasma urealyticum (Uu) ,Mycoplasma hominis (Mh) and Chlamydia trachomatis(Ct) in patients with urogenital tract infection ,and to analyze the drug resistance of mycoplasma .Methods The myco‐plasma and its drug sensitivity of urogenital tract specimens were detected by culture‐identification‐susceptibility integration kit .Ct was detected by using immunochromatographic method .Results Among 497 cases of specimens ,there were 194 cases of simplex Uu infection (39 .0% ) ,9 cases of simplex Mh infection (1 .8% ) ,73 cases of Uu and Mh mixed infection (14 .7% ) ,and 54 cases of Ct infection (10 .9% ) .There were 38 cases of Ct and Uu mixed infection (7 .6% ) in the Ct positive specimens .The isolated myco‐plasmas were sensitive to doxycycline ,josamycin and minocycline ,but resistant to other drugs in varying degrees .Conclusion There are both simplex infection and mixed infection of chlamydia and mycoplasma in the patients with urogenital tract infection . The clinical use of antibiotics should be reasonable based on pathogen detection and drug susceptibility test results .

OBJECTIVE:To establish a method for bacterial endotoxin test of Boric acid rinse.METHODS:According to appendix volumeⅡof Chinese Pharmacopoeia(2005 edition),the tachypleus amebocyte lysate(TAL)method was applied for the pre-inference test and interfence test to investigate non-inference concentration,pH value and sensitivity of TAL.RESULTS:Boric acid did not interfere with TAL and the reaction of bacterial endotoxin at 2 times diluted concentration with pH value adjusted above 6.The sensitivity 0.25 EU?mL-1 of TAL was available.CONCLUSION:The TAL method can control the limit of bacterial endotoxins in Boric acid rinse.

Background and objectives To investigate the effect of hepatocyte growth factor (HGF) on left ventricular (LV) remodeling after acute myocardial infarction (AMI). Methods AMI was produced by ligation of proximal left anterior descending coronary artery(LAD) in 12 mongrel canines. These animals were randomized into 2 groups. In HGF group (n=6), canines were injected with pcDNA3-HGF lml (about 300ug) at the margin of infarcted myocardium; in control group (n=6) canines were injected with equal volume of normal saline. Cardiac function and left ventricular remodeling were evaluated with echocardiography at 1, 4, 8 weeks after MI. LV myocardium specimens were obtained at 8 weeks and stained with hematoxylin and eosin for histological examination or with sirius red to assess the collagen content. Results Compared with control group, LVEF in HGF group was significantly higher at 4 weeks (49.61+6.66 vs 39.84+6.39; P＜0.05) and at 8 weeks (51.57+8.53 vs 40.61+7.67; P＜0.05) after AMI, while LVESV was significantly lower in HGF group than that in control group at 8 weeks after AMI (18.98+3.47 vs 25.66+5.86; P＜0.05). Posterior left ventricular wall thickness decreased significantly from 1 wk to 8 wks after AMI in control group, while remained unchanged in HGF group. Compared with control group, histological examination showed more neovascularization and less scar, and sirius red staining indicated higher volume of type Ⅲ collagen (7.10±4.06% vs 3.77±1.09%; P＜0.05) and lower collagen Ⅰ/Ⅲ ratio value (1.11±0.52 vs 2.94±2.48; P＜0.05)in HGF group. Conclusion HGF gene transfer might improve cardiac function and LV remodeling after acute myocardial infarction by stimulating angiogenesis, reducing fibrosis, and reducing myocardial scarring.

Objective To investigate the relationships between the expressions of intercellular adhesion molecule 1(ICAM-1),heat shock protein 70(HSP70) and acute rejection after pancreas allotransplantation in miniature swine.Methods Ultrasound guided biopsies of the pancreas and histopathologic examination were performed after the miniature swine models of pancreas allotransplantation were established.According to the histological change,the samples were divided into group Ⅰ(no rejection),group Ⅱ(mild acute rejection),group Ⅲ(moderate acute rejection)and group Ⅳ(serious acute rejection).The expressions of ICAM-1 and HSP70 were analyzed with immunohistochemistry and imaging.Results The expressions of ICAM-1 and HSP70 were increased when acute rejection occurred after pancreas allotransplantation in miniature swine.The levels of ICAM-1 and HSP70 were sequentially high when acute rejection was serious.Conclusion ICAM-1 and HSP70 were involved in pancreas allograft rejection and useful for early diagnosis of acute rejection after pancreas transplantation.

Objective To establish a successful model for pancreaticoduodenal allotransplantation with enteric drainage in piglet and surveillance acute rejection by biopsy.Methods We implemented the model same as protocol of clinical pancrease transplantation.40 piglets were divided into donors and recipients randomly.20 times experimental operation were finished. The total pancreas with a short segment of duodenum was transplanted to a recipient by anastomoses of vessels to the recipient iliac vessels and small bowel segment to the jejunum with enteric drainage. During operation, mean arterial pressure(MAP), central venous pressure(CVP)were monitored. The levels of amylase activity in blood were investigated.Doppler color blood flow ultrasonography was used to evaluate blood flow of pancreatic allografts,and ultrasound-guided percutaneous pancreas allograft biopsy and histopathologic examination were performed.Results The successful rate of operation was 90%. Recipient's MAP was decreased after the reopening of inosculated blood vessels.(P

In order to construct a eukaryotic co-expression plasmid containing membrane-anchored Sjcl4FABP and Sjc26GST genes and identify their expression in vitro, Sj14 and Sj26 genes were obtained by RT-PCR with total RNA of Schistosoma japonicum adult worms as the template and cloned into eukaryotic expression plasmid pVAC to construct recombinant plasmids pVAC-Sj14 and pVAC-Sj26. Then a 23 amino-acid signal peptide of human interleukin-2 (IL-2) upstream Sj14 or Sj26 gene and a membrane-anchored sequence containing 32 amino-acids of carboxyl-terminal of human placental alkaline phosphatase (PLAP) downstream were amplified by PCR as the template of plasmid pVAC-Sj14 or pVAC-Sj26 only to get two gene fragments including Sj14 gene and Sj26 gene. The two modified genes were altogether cloned into a eukaryotic co-expression plasmid pIRES, resulting in another new recombinant plasmid pIRES-Sj26-Sj14. The expression of Sj14 and Sj26 genes was detected by RT-PCR and indirect immunofluorescent assays (IFA) when the plasmid pIRES-Sj26-Sj14 was transfected into eukaryotic Hela cells. Restriction enzyme analysis, PCR and sequencing results revealed that the recombinant plasmids pVAC-Sj14, pVAC-Sj26 and plRES-Sj26-Sj14 were successfully constructed and the expression of modified Sj14 and Sj26 genes could be detected by RT-PCR and IFA. A bivalent membrane-anchored DNA vaccine encoding Sj14 and Sj26 genes was acquired and expressed proteins were proved to be mostly anchored in cellular membranes.

Objective To investigate the current situation and the differences of nurse work environment between a military hospital and a civilian hospital.Methods By quota sampling,a questionnaire survey was conducted among a total of 1244 nurses in a military hospital and a civilian hospital in Urumqi.Results The nurse work environment in both hospitals was favorable overall.The total scores of nursing environment and subscale scores of the military hospital were lower than those of the civilian hospital.There were no statistical differences between the two hospitals on their perceptions of the collaboration with physicians as well as leadership and ability of nurse manager.Conclusions The nurse work environ-ment in the civilian hospital was a little more favorable than the military one.Both of their managers,espe-cially the military hospital,should pay more attention to manpower and resources as well as nurses'involve-ment in hospital affairs in order to further optimize nurse work environment.

Objective To contrast the radiation dose to patients of 320 row CT coronary artery imaging (CTCA) and coronary angiography(CAG) .Methods 90 patients random sampled with 320 row CTCA were group A .Among of them ,7 patients with cal-cification integral scanning (CI) were A1 group;43 patients with CT coronary artery imaging/cardiac function analysis scanning (CTCA/CFA) were A2 group;40 patients with Prospective scanning (Prospective CTA ) were A3 group;139 patients random sam-pled with CAG were group B .Results Radiation dose had statistically significant between Group A and group B ,A1 and B ,A2 and B ,A1 and A3 ,A2 and A3(P<0 .05) .radiation dose had no statistically significant between Group A3 and group B ,A1 and A2(P>0 .05) .Conclusion In order to reduce the radiation dose to patients ,the prospective CTCA was used in the patients need 320 row CTCA as far as possible .

Objective To discuss the nursing intervention of Rey syndrome in children patients.so as to search effective nursing measures.Methods 12 children patients with Rey syndrome received comprehensive nursing treatment with reducing intracranial pressure and were under continuous close observation.Slightest changes were feeded back timely,then effective nursing measures were taken to stabilize their illness,patients also received dietary therapy,medication nursing and rehabilitation training,etc.Results All patients ameliorated after 3 to 7 days,and achieved clinical recovery after 20 to 30 days without sequelae.Conclusions Early diagnosis and correct effective nursing care can improve curative rate and avoid the occurrence of complications and sequelae.