0.05);Compared with the control group, the time of first stage(403?135) min and the total time of labor(455?148) min were shorter ( P

Objective To investigate the change of serum prealbumin (PA) and C-reactive protein (CRP) in burn patients and its clinical significance.Methods Sixty eight cases of burn patients were divided into two groups,group Ⅰ [n=38,with total burn surface area less than 15% of total body surface area (TBSA)] and groupⅡ(n=30,with total burn surface area more than 50% of TBSA). Thirty healthy people were set as healthy control group. Particle enhanced immunoturbidimetic assay was applied to detecting the serum levels of PA and CRP for all subjects on the day 1 after burns.Results The CRP level was much higher and serum level of PA was much lower in group Ⅱ than that of group Ⅰ,respectively. There existed significant negative correlation between PA and CRP level.Conclusion The level of serum PA decreases significantly along with total burn surface area expansion. PA can be used as a sensitive marker for estimation of inflammation degree after burns.

Objective:To establish a method for the determination of salicylic acid in the compound sulfur ointments by ultra-per-formance liquid chromatography (UPLC). Methods:An Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5 μm)column was used, the mobile phase was methanol-0. 1 mol·L-1 sodium dihydrogen phosphate (30∶70), the detection wavelength was 231nm, the column temperature was 35℃, the flow rate was 1. 0 ml·min-1 and the injection volume was 10μl. Results:The linear range of salicylic acid was 11. 96-59. 80 μg·ml-1(r=0. 999 9), and the average recovery was 99. 72%(RSD=1. 28%,n=9). Conclusion:The method is accurate, stable and specific, and suitable for the determination of salicylic acid in the compound sulfur ointments.

Objective To discuss the comparability of ALT, AST, CK and LDH results by ap- plying three different vacuum tubes (no additive tubes, separation gel coagulation promoting tubes and other coagulation promoting tubes) for venous blood samples, and to provide evidence for clinical tests. Methods Fast venous blood from 26 healthy blood donors were collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. Olympus AU5400 Biochemical Analyzer was applied to examining serum ALT, AST, CK and LDH for all pre- pared serum samples. Results There was no statistical difference of serum ALT, AST, CK and LDH level collected in test tubes without additives, separation gel coagulation promoting tubes and other co- agulation promoting tubes. With the no additive tubes as the standard tubes, the above result bias be- tween separation gel coagulation promoting tubes and other coagulation promoting tubes were clinically acceptable. Conclusion There is credible comparability of serum ALT, AST, CK and LDH level a- mong different kinds of venous blood samples collected in test tubes without additives, separation gel coagulation promoting tubes and other coagulation promoting tubes. The separation gel coagulation promoting tubes and other coagulation promoting tubes can substitute test tubes without additives for ALT, AsT, CK and LDH determination.

Objective To study the role of glucocorticoid (GC) in treating patients with cyto- megalovirus (CMV) pneumonia following renal transplantation.Methods There were 75 cases CMV pneumonia following renal transplantation during one month (3 cases),2-6 months (64 cases) and more than 6 months (8 cases).All patients were subjected to the comprehensive treatments including anti-virus therapy.In 47 cases,GC was given (GC group),and in the rest 28 cases,GC was not administered (non-GC group).Results In GC group,40 cases (85.1%) were cured and there were 7 deaths (14.9%).In non-GC group,17 cases (60.7%) were cured and there were 11 deaths (39.3%).There was significant difference between two groups (P

Adolescent ; Adult ; Aged ; Female ; Humans ; Induction Chemotherapy ; Intercellular Signaling Peptides and Proteins ; metabolism ; Leukemia ; metabolism ; therapy ; Male ; Middle Aged ; Proto-Oncogene Proteins ; metabolism ; Receptor Protein-Tyrosine Kinases ; metabolism ; Young Adult

Intrauterine growth retardation (IUGR) is one of the most commonly encountered developmental toxicity, which could lead to perinatal morbidity and mortality, be also extended from the fetus to adulthood, and seriously affect the quality of the population. Caffeine widely exists in a variety of daily beverages and some drugs. Its consumption is increasing year by year. Caffeine intake during pregnancy is one of the risk factors for IUGR. However, its mechanism of adverse outcome based on embryonic research is still unclear. In this paper, the possible mechanisms of caffeine-induced IUGR focusing on 3 important factors-the mother, placenta and fetus were explored. Caffeine's impact on the mother is the chronic activation of renin-angiotensin system; on the placenta, caffeine induces cell damage or the failure of the cell proliferation/apoptosis balance, leading to blockage of blood supply to the placenta; caffeine is also capable of directly affecting fetal development through interfering its neuroendocrine.

AIM: To investigate the protective effect of ischemic-preconditioning under the mild hypothermia against small intestine ischemia-reperfusion injury in rats and its mechanism. METHODS: Thirty-two rats were randomized into 4 groups (8 rats in each group): sham operated group (Sham), ischemia-reperfusion (I/R) group, ischemic-preconditioning (IP) group, mild hypothermia ischemic-preconditioning (MHIP) group. The wet/dry ratio, Ca 2+ -Mg 2+ -ATPase activity in intestine tissue, the malondialdehyde (MDA) content, activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and total antioxdase (TAX) in blood were determined. Ultrastructure, Bcl-2 and Bax expression in intestinal mucosa tissue were also observed. RESULTS: After I/R, the intestinal tissue wet/dry ratio, the content of MDA, LDH activity, the optic density of Bcl-2 and Bax proteins were significantly higher in I/R group than those in sham group (P

Technology readiness level (TRL) refers the general maturity level of a single technology or technical systems (including technology,component or subsystem) during the process of development.Base on the original TRL proposed by NASA,with reference to medical device TRL modified by US Department of Defense and NATO,this study modified TRL definition and description for advanced medical devices with consideration of R&D,review and approval characteristics of advanced medical devices in China.

Objective To study the effect of Licofelone,a novel non-steroid anti-inflammatory drug,on the expression of Fractalkine induced by interleukin-18(IL-18) in mesangial cells.Methods Rat mesangial cells were cultured and divided into IL-18 stimulated group,Licofelone-treated group and normal control group.The cells in IL-18 stimulated group were stimulated by 10 ?g/L IL-18 for 24 h.In Licofelone-treated group,ahead of exposure of IL-18 for 24 h,cells were treated with Licofelone in the doses of 10,50 and 100 ?mol/L for 30 min.Additionally,the mesangial cells without treatment of IL-18 and Licofelone were used as normal control group.Reverse transcription-polymerase chain reaction(RT-PCR) was used to measure the level of Fractalkine mRNA.The expressions of Fractalkine protein in every group were detected with enzyme linked immunosorbent assay (ELISA).Results In normal control group,the expression level of Fractalkine mRNA was 179.0?21.0.After exposure of IL-18 for 24 h,the level of Fractalkine mRNA was 1 220.1?185.7,which was higher than that in normal control group (t=9.646 P