Objective To observe the effect of tai chi chuan on anneal dyspnea and endurance stamina of patients with chronic obstructive pulmonary disease (COPD).Methods 80 COPD patients were randomly divided into control group (n=40) and training group (n=40). The common therapy of two groups was the same; but the training group was added with Chen style tai chi chuan training for 30 minutes one day. At the same time, all patients were evaluated about the dyspnesa exponent before and after 3 months training.Results There were no significant differences in FEV1(%), FEV1/FVC(%),heart rate and breath frequency/minute before and after treatment (P>0.05) between two groups, but in dyspnea exponent, there was a significant difference between two groups (P<0.05).Conclusion Tai chi chuan training can reduce the symptom of COPD patients.

Objective To study the impact of gender,gender role and gender traits on depression in adolescents.Methods A sample of 1330 middle school students from seven provinces in China completed Beck Depression Inventory (BDI) and Gender Traits Inventory.Results 27.1 percents of participants displayed the symptoms of depression.The mean score of males of BDI was 10.04 ± 8.38 while that of females was 10.33 ± 8.37,indicating that there was no sex differences in depression of adolescents(P=0.535).The participants could be classified into 4 sex role types:masculine,feminine,androgynous and undifferentiated,and the BDI mean score of each type was 9.51 ± 7.66,12.30 ± 8.81,9.14 ± 8.10,10.72 ± 8.66,and significant differences were found among adolescents with different gender roles (P =0.000).BDI scores of the feminine adolescents were the highest among the four gender roles,which were significantly higher than scores of the masculine and androgynous adolescents (P =0.000).Regression analysis indicated that adolescents with more positive masculine traits tended to be less depressive (t =-4.966,P ＜ 0.01) while adolescents with more negative masculine (t =2.574,P ＜ 0.01) or feminine (t =10.566,P ＜ 0.01)traits tended to be more depressive.Conclusion Gender role rather than gender has greater impacts on adolescent depression,and specifically,positive masculine traits represent protective factors for depression.

Objective To analyse the role of haptoglobin in the immunomodulatory in patients with premature coronary heart disease.Methods Clinical data of 60 premature coronary heart disease patients and 40 healthy people was analysed.The general information and serume haptoglobin,hs-CRP and IL-6 were compared.Results There were no statistically difference between the two groups of the general information such as sex,years,BMI,hypertension and diabetes (P>0.05),excepts the smoking history(27/33 vs 9/31,χ2=3.324,P<0.05).Hp,hs-CRP and IL-6 levels in the observer group were signifi-cantly higher than the control group,the difference was statistically significant (97.76±23.08 mg/L vs 65.81±19.42 mg/L,10.43±3.81 mg/L vs 3.05±0.94 mg/L,16.43±4.59 pg/L vs 4.62±1.83 pg/L,P<0.001).Correlation analysis showed that in the observer group Hp and hs-CRP showed a significant positive correlation (r=0.518,P<0.001),and the same results was found between IL-6 and Hp (r= 0.291,P=0.024).Conclusion Haptoglobin level was significantly in-creased in patients with coronary heart disease,which suggested that haptoglobin played a role in the development of coro-nary heart disease,and its level was correlated with IL-6.

Objective To study the roles of regulated upon activation normal T cell expressed and secreted(RANTES) and monocyte cbemoattractant protein-1 (MCP-1) in human immunodeficien- cy virus-1(HIV 1) infected patients.Methods RANTES and MCP-1 in HIV-1 infected patients, including treated and untreated groups,and healthy control group were determined by enzyme-linked immunosorbent assay(ELISA).The recombinant plasmids,hMCP-pcDNA3.1,bRANTES- peDNA3.1 and hMCP/bRANTES-pcDNA3.1,were constructed and transfected into CHO cells to overexpress the corresponding recombinant proteins,whose chemoattract function was then studied. Results The level of RANTES was (164.3?21.3) pg/mL in healthy control group,(1 224.1?62.0) pg/mL in untreated group and (475.3?36.2) pg/mL in treated group.The level of MCP-1 was (90.6?28.5) pg/mL in healthy control group,(335.0?30.3) pg/mL in untreated group and (807.2?62.6) pg/mL in treated group.In HIV-1 infected patients,the levels of RANTES and MCP-1 were significantly increased.After highly active antiretroviral therapy (HAART),the level of RANTES declined,but MCP-1 increased further.Western blot assay revealed that the three recombinant proteins could be recognized by monoclonal antibodies respectively.All of them could chemoattract human peripheral blood mononuclear cells(PBMCs).And the chemoattractant potency of MCP/RANTES fusion protein was stronger.When the recombinant proteins were used with con- centrations as 50,200,400 and 800 pg/mL,respectively,the number of PBMCs chemoattracted by MCP/RANTES fusion protein was 52?10~4/mL,102?10~4/mL,132?10~4/mL and 184?10~4/mL; the number of PBMCs chemoattracted by RANTES was 27?10~4/mL,51?10~4/mL,65?10~4/mL and 96?10~4/mL;the number of PBMCs chemoattracted by MCP-1 was 18?10~4/mL,44?10~4/mL, 54?10~4/mL and 74?10~4/mL.Conclusion RANTES and MCP-1 may both be involved in the HIV infection process and host immunological reaction against HIV.

OBJECTIVE: To study the in vitro metabolism and enzyme kinetics of di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride in rat liver microsomes, and to identify the major cytochrome P450 isozymes involved in the metabolism of di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride in rat liver microsomes. METHODS: By optimizing the incubation conditions of di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride in rat liver microsomes, the enzyme kinetics in different enzyme sources was researched; the cytochrome P450 isozymes involved in metabolism of di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride were preliminarily explored through in vitro inhibition experiments. RESULTS: Different enzyme source metabolism experiments showed that between phenobarbital(PB) and desamethasone(Dex) induction groups and blank control group there had significant differences, but between the BNF group and blank control group there had no significant difference; the inhibition experiments revealed that ketoconazole had strong inhibition effect on di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride metabolism. CONCLUSION: CYP3A plays a leading role in di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride metabolism, and CYP2C9 may be partly involved. CYP1A has no catalysis action on metabolism of di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride. The Results suggest that attention should be paid to the possibility of drug interactions when di-n-butyl-(4-chlorobenzohydroxamato) tin(IV) chloride is combined with the drugs metabolized by above-mentioned isozymes.

OBJECTIVE: To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit. METHODS: The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework. RESULTS: The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA. CONCLUSION Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.
Asian People/genetics* ; China ; Chromosomes, Human, Y ; DNA/genetics* ; DNA Fingerprinting/standards* ; Databases, Nucleic Acid ; Female ; Forensic Genetics/methods* ; Genotype ; Humans ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Reproducibility of Results ; Sensitivity and Specificity

BACKGROUND: Body temperature has significant effects on brainstem auditory evoked potentials (BAEP). The wave latency of BAEP is the sensitive and objective index for reflecting the effects of body temperature on the synaptic transmission and nerve fiber conduction. The wave origin of electrocochleogram (ECochG) and the effects of body temperature on it need to be probed into to further define its wave origin so as to guide its clinical application.OBJECTIVE: To observe the effects of body temperature on ECochG and provide important basis for further defining its wave origin and guiding its clinical application.DESIGN: A randomized block design.MATERIALS: This study was performed in the Department of Physiology, Medical College of Jinan University between July and September 2002. Forty adult guinea pigs were randomly divided into hypothermia group (n=20) and hyperthermia group (n=20).INTERVENTIONS: The body temperature of the guinea pigs was decreased or increased step by step with their body contacted with physical method. and the changed rate of body temperature was kept as decreased or increased by 1 ℃ every 5-10 minutes. BAEP and ECochG were detected when the body temperature was changed by 1 ℃.MAIN OUTCOME MEASURES: The PL and IPL of BAEP and ECochG were observed.RESULTS: Finally, 37 guinea pigs were involved in the analysis of relonged as the body temperature was decreased step by step from 36 ℃ to 25 ℃, and shortened as the body temperature was increased gradually from 36 ℃ to 42 ℃. The PL and Ⅰ-Ⅳ IPL prolonged or shortened more significantly with the temperature changed. The PL of wave N1, N2 and N3 and N1-N3 IPL of ECochG also prolonged or shortened as the body temperature perature,the total prolonged and shortened values of Ⅰ -Ⅳ IPL representing the central transmission time of brainstem were much greater than that of wave Ⅰ PL representing the peripheral conduction time, .and the total prolonged and shortened values of thePL of wave Ⅱ , Ⅲ and Ⅳ originating from the brainstem centers were much greater than that of wave Ⅰ originating fromthe auditory nerve. The total prolonged and shortened values of N1-N3 IPL were greater than that of wave N1 PL representing the peripheral conduction time, and the total prolonged and shortened values of the PL of wave N2 and N3 were much greater than that of wave N1 originating from the auditorynerve.CONCLUSION: The significant effects of body temperature on ECochG are similar to those of BAEP, and the origin of wave N2 and N3 of ECochG is higher than that of wave N1 that originates from the auditory nerve.

AIM： To study the effects of nabumetone on immunological fuctions in mice METHODS： After nabumetone was given ig to mice， the weight of thymus and spleen， phagocytizing function of macrophages， PHA－ induced lymphocyte transformation were observed respectively in mice RESULTS： The weight of thymus and spleen was decreased， the phagocytizing function of macrophages was reduced， PHA－ induced lymphocyte transformation was markedly inhibited with nabumetone in mice CONCLUSION： The immunological function in mice was significantly inhibited with nonsteriod anti－ inflammatory drug－ nabumetone

A total of 104 patients with cervical squamous cell cancer (Figo Ⅰ b2-Ⅱ b) were recruited during March 2006 and October 2011.One group (A,n =50) received preoperative intravenous neoadjuvant chemotherapy while another group (B,n =54) had interventional arterial chemotherapy plus bilateral uterine artery embolization.Surgery was performed for those with a change of cancer size either as complete or partial remission at Week 2 post-chemotherapy according to the WHO evaluation criteria.Otherwise radiotherapy was implemented.The ratio of surgery after chemotherapy was 70％ (n =35) in Group A versus 87％ (n =47) in Group B (P ＜ 0.05).For Group A,there were blood vessel invasion (n =6,17％),lymph node metastasis (n =8,23％) and positive margin (n =1,3％).For Group B,(n =7,15％),(n =9,21％) and (n =1,2％).There was no significant inter-group statistic difference (P ＞ 0.05).Preoperative chemotherapy is effective for cervical squamous cancer (tumor diameter ＞ 4 cm)with fewer side effects.

Objective To explore the relationship between viral encephalitis(VE)and Borna disease virus(BDV)infection,and discuss the clinical features of viral encephalitis patients infected by BDV.Methods The p24 gene fragment of BDV in cerebrospinal fluid mononuclear cells(CSFMC)from 32 patients with viral encephaliris and 34 healthy individuals were examined by fluorescence quantitative nested reverse transcriptase polymerase chain reaction(FQ-nRT-PCR),in which, β-actin was used as internal reference. The clinical manifestations of patients with positive BDV p24 were watched. And the gene sequence,homology and amino acid sequence of positive products were analyzed. Results The positive rate(4/32,12.5%)of BDV p24 in CSFMC of patients with viral encephalitis was significantly higher than that of healthy individuals(0/34,0%),P＜0.05,and the numbers of copies of BDV p24 in CSFMC of VE patients were more than 102/μl. Compared with the sequences of the standard strain Ⅴ and the virus strain H1766 of BDV from horse supplied by GenBank,the homology of the gene sequence of positive product of CSFMC was 95.35% and 98.84% with gene mutation in 4 sites(nt1649 T→C, nt1656 G→A,nt1670 C→T, nt1676 C→T). The genetic relationship between the positive product and the virus strain BDV from horse was the nearest. The clinical features of VE patients with positive BDV p24 were mainly by mental and behavior disorders. Conclusion The occurrence of VE patients in the city of Zunyi in Guizhou province may be associated with BDV infection. The clinical features of VE patients with positive BDV p24 were mainly mental and behavior disorders.