Taking the modern occupation education concept as the guide,oriented by adapting to the demand of occupation post,we established a new open education mechanism of medical occupation education to promote the reform of personnel training mode,curriculum and teaching content to explore the effective way of the sustainable development of local medical occupation education.

Transient receptor potential (TRP) is a kind of novel Ca2+ permeant channel.It is later found that TRP channels are expressed broadly in many organisms,tissues and cell types.It is involved in the regulation of sensory conduction and cell signal transduction.Further studies are required to assess which TRPC6(a member of TRPC subfamily) is associated with the cytosolic Ca2+ levels,development and progression of cancer and tumor cell cycle.TRPC6 may be regarded as new targets for the treatment of cancer.

For stage Ⅱ colon cancer patients after surgery,the benefit of adjuvant chemotherapy remains controversial.Several studies indicate that the patients with high-risk stage Ⅱ colon cancer can benefit from adjuvant chemotherapy after surgery.According to the clinical and pathological features,the stage Ⅱcolon cancer patients including T4 lesion,perforation,peritumoral lymphovascular invasion can benefit from the adjuvant chemotherapy of oxaliplatin.In the horizontal of molecular and genetic levels,the stage Ⅱ colon cancer patients can express high microsatellite instability (MSI-H) and cannot benefit from the chemotherapy of Fluorouracil.The guiding functions of 18q loss of heterozygosity (18q LOH),Cx43 and gene expression profiling in adjuvant chemotherapy are still unclear,and need further study.

One of the biggest differences between ordinary undergraduate education and higher vocational education is more emphasis on the cultivation of students' vocational skills so as to adapt to so-cial demand and employment. In order to overcome the deficiencies in current teaching outline of voca-tional education and to change from subject knowledge teaching to applied skill cultivating , medical and health curriculum standard including 'curriculum philosophy, curriculum goal, curriculum content, curriculum implementation' four elements was formulated based on concepts of 'education as base , practice orientation and lifelong learning' and cultivation mode combining industry standard with cur-riculum teaching goal and combing work with study. All measures taken above were aimed at promoting coordinated development of students' knowledge, ability and emotion and satisfying the social demand for talent.

Objective Perioperative fluid therapy has always been the most basic and important measure to maintain the stability of patients'cyclic system.Colloid and crystalloid,as common perioperative fluid,play different roles in clinical application due to the different molecular sizes.In the recent years,besides their contribution to maintain the stability of cyclic system,scientists have begun to pay attention to the effect on coagulation,especially their comparison.This paper mainly discusses the study status of the influence on coagulation produced by different liquids.

Objective To investigate the changes of the level of oxidative stress and cell apoptosis of secondary brain injury after traumatic brain injury,and the influence of brain-derived neutrophic factor on these parameters in rats,as well as its potential mechanisms.Methods A total 84 adult and healthy male rats was divided randomly into 2 groups:control (n =42) and traumatic brain injury (TBI) groups (n =42).The brain-derived neurotrophic factor (BDNF) group was induced using improved Feeney method and was received abdominal injections of BDNF (0.5 μg/μl) immediately after injury,the control group were received abdominal injections with the same dose sodium chloride injection immediately after injury and repeat one time everyday until the rats was killed.Each group was divided into seven subgroups by sacrificed time after injury,those are 1 h,3 h,6 h,12 h,24 h,3 d,and7 d,each subgroup got6 rats.Each subgroup was randomly selected three rats after being killed.The water content,superoxide dismutase (SOD),malonic aldehyde (MDA),and glutathione (GSH) of rats were measured contusion peri tissues brain tissue.Specimens were taken from left three rats of subgroup,which was part of the brain tissue.The expression of NF-κB p65,around the brain tissue with immunohistochemical methods were detected.TdT-mediated dUTP nick-end labeling (TUNEL) method was used to observe the peri cell apoptosis after brain contusion.Results NF-κB p65 was expressed obviously around the lesion in 1h group,and strongly expressed in TBI-3 h-12 h,and reached a peak in 24 h after the injury,while NF-κB p65 expression reduced in TBI-3 d-7 d,and still in high expression.NF-κB p65 expression strongly correlated with the degree of cerebral edema (r =0.651,P ＜0.05).For two groups,NF-κB p65 expression strongly correlated with the level of MDA (r1 =0.947,P ＜0.01;r2 =0.961,P ＜0.01).Conclusions Changes of NF-κB protein expression after brain injury were involved in a series of pathological processes of secondary brain injury,such as oxidative stress,and apoptosis,brain-derived neutrophic factor is probably through inhibit oxidative stress levels,control apoptosis,prevent the development of vasogenic cerebral edema,and reduce or mitigate secondary brain injury.

Objective: To explore the clinical efficacy of positive airway pressure biphasic (BiPAP) non-invasive ventilation for treating the patients with severe pre-eclampsia combining acute heart failure (AHF).
Methods: A total of 84 patients with severe pre-eclampsia combining AHF treated in our hospital from 2008-01 to 2014-12 were retrospectively studied. The patients were divided into 2 groups: Control group, the patients received routine treatment for pre-eclampsia and AHF,n=41 and Observation group, based on routine treatment, the patients received assistant BiPAP ventilation,n=43. The changes at before and 3h after treatment of cyanosis, dyspnea, pulmonary rales, heart rate (HR), respiratory rate (RR), arterial oxygen saturation (SaO2), arterial partial pressure of oxygen (PaO2), arterial carbon dioxide partial pressure (PaCO2), pH value and plasma levels of BNP were compared between 2 groups.
Results:①Comparison of before vs after treatment in both groups: HR (times/min) in Control group (90±8 vs 110±14) and Observation group (80±6 vs 112±12); RR (times/min) in Control group (24±5 vs 33±8) and Observation group (18±4 vs 35±7); PaCO2 (mmHg) in Control group (41.3±4.3 vs 48.4±5.6) and Observation group (29.7±5.4 vs 47.8±3.9); BNP (ng/L) in Control group (87.50±8.00 vs 133.00±8.00) and Observation group (69.50±8.30 vs 138.00±6.92); SaO2 (%) in Control group (93.0±3.7 vs 80.5±4.7) and Observation group (97.1±3.4 vs 81.2±4.2); PaO2 (mmHg) in Control group (80.3±5.8 vs 80.5±4.7) and Observation group (89.1±6.2 vs 53.2±5.4), allP<0.05.②After treatment, compared with Control group, Observation group presented obviously decreased HR, RR, PaCO2 and BNP; signiifcantly increased SaO2 and PaO2, allP<0.05. PH was similar between 2 groups,P>0.05.
Conclusion: Assistant BiPAP ventilation may treat the patients with severe pre-eclampsia combining AHF, it could improve HF symptom and hypoxia. The clinical signiifcance should be conifrmed by further investigation.

Objective To evaluate t he efficacy of fluoxetine in treating tinnitus patients with depress. Methods A randomized,open-labeled,controlled clinical tri al was conducted.There were 31 patients in control group and 26 p a tents in fluoxetine group.The efficacy was assessed by tinnitus patients with Ra ting Scale and Hamilton Depression Rating Scale (HAMD). Results The total effective rate of fluoxetine group was 71.11%, and there was significant improvement rate in depression after 1 month f luoxetine treatm ent. Conclusion Fluoxetine is an effective drug for tinnitus p atients with depression.

Objective To study the effect of CsA in a therapeutic dose on the hepatic ultrastructure in rats.Methods Twenty-four SD rats were divided into 3 groups.The control group was orally administered olive oil,and the other 2 groups were orally given CsA 25 mg/kg and 50 mg/kg daily respectively.After 2 weeks.the liver samples were taken for ultrathin sections.The stereological method was used to quantify some hepatic ultrastructural parameters including volume density (Vv),surface density(Sv),numerical density(Nv) and specific surface(δ).Results CsA caused an increases of Vv and Sv and a decrease of δ in endoplasmic reticulum;a decreases of Vv and Nv in microbody;decreases of Vv in Golgi complex and glycogen inclusions;an increases of Vv and Nv in lipid inclusions.Conclusion CsA in a therapeutic dose could cause the change of hepatic ultrastructure of rat.

Carbon monoxide(CO)has a significant anti-inflammatory action.Carbon monoxide releasing molecule(CORM),as a new donor of CO,is used widely in biological study.The article reviewed the antiinflammatory action of CO and the progress of CORM.