Thalassemias are a group of inherited disease which is caused by globin gene defects.Identification and quantification of hemoglobin play a very important role for the screening and diagnosis of thalassemia,especially the quantitative analysis of Hb A2 and Hb F.Through past 60 years,the methods for Hemoglobin analysis have been developed from starch gel electrophoresis,paper electrophoresis,cellulose acetate electrophoresis,agarose gel electrophoresis,column chromatography,high-performance liquid chromatography till capillary electrophoresis,Its development closely associates with the research on thalassemia.No matter what kind of methods is chosen,quality control is very important.

The application of radio frequency identification(RFID) and wireless network to neonates' management can effectively avoid occupying infants by mistake.This technology can provide an advanced accessorial instrument in hospital management and upgrade the hospital's professional level.

OBJECTIVE:To establish a method for test of bacterial endotoxin on cefalotin sodium for injection by gel clot.METHODS:Cefalotin sodium for injection was diluted in water for determination of bacterial endotoxin and allocated to sample negative and sample positive controls to determine its maximum noninterference concentration;Then bacterial endotoxins were diluted into solutions of different concentrations with the test solutions,and interference test was carried out by using tachy?pleus amebocyte lysate sensitivity compound nucleus.RESULTS:The maximum noninterference concentration of cefalotin sodium for injection was6.68mg/ml;And cefalotin sodium at the concentration of1.67mg/ml did not interfere with the test for bacterial endotoxins.CONCLUSION:The present method can replace pyrogen test with rabbit for examination of bacterial endotoxin in cefalotin sodium for injection.

Objective To study on the effect of joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract.Methods 78 vitreoretinal diseases patients complicated with cataract(86 eyes)were recruited.All the cases accepted vitrectomy and cataract extraction at the same time.The vision improving situation and postoperative complications of the treatment were analyzed.Results After the joint surgery of anteriorposterior,the visual improvement rate was 88.4％(76/86 eyes),and with unchanged vision in 7.0％,and decreased vision in 4.7％.In 4 patients with decreased vision,1 case was a secondary neovascular glaucoma,and 2 cases were macular hemorrhage or hiatus,1 case was a retinal detachment.In phase Ⅰ,72 eyes did not implant artificial lens,63 eyes(87.5％)got postoperatively improved vision,and 14 eyes implanted artificial lens,and all of them got improved vision(100.0％),and the difference was not statistically significant(P ＞ 0.05).The total postoperative complication rate was 10.5％(9/86 eyes).Conclusion Joint surgery of anterior-posterior in the treatment of vitreoretinal diseases complicated with cataract had determined curative effect and lower complication rate,which deserved further study and widely using.

Objective To understand the total score of health management knowledge demand and self evaluation score of known health management knowledge,in order for hospitals to take more reasonable and effective health management education for patients after discharge.Methods From March to May of 2012,700 patients in a certain three level of first-class general hospital were selected before their discharge,using self-made health management questionnaire for investigation.The total score of health management knowledge demand and score of known health management knowledge among 700 patients with different demographic characteristics underwent statistics.Results After investigation,the results showed that patients with different nationalities,payment method,the average monthly income,family history,residence and intend demonstrated statistical significance in total score of health management knowledge requirement.Patients with different occupation,culture degree and accept frequency showed statistical significance in known health management knowledge.Conclusions According to the current investigation of total score of different health management knowledge demand and known health management knowledge in patients with different demographic characteristics,the hospital should take corresponding adjustment for health management mission to ensure the health management quality of patients after discharge,and improve the health management level of patients after discharge.

China ; Humans ; Liver ; surgery ; Translational Medical Research

Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Glomerulonephritis, IGA ; diagnosis ; drug therapy ; Humans ; Kidney Failure, Chronic ; diagnosis ; drug therapy ; Medicine, Chinese Traditional ; Nephrotic Syndrome ; diagnosis ; drug therapy ; Phytotherapy

Aim To investigate the effect of allopurinol and benzbromarone on serum level of uric acid, hepatic xanthine oxidase(XOD) activity and intestinal expression of glucose transporter(GLUT) 2 and 5 in rats with fructose-induced hyperuricemia.Methods Wistar rats were fed with 10% fructose in drinking water for consecutive 8 weeks to induce HUA.Treatment with 5 mg·kg-1 allopurinol or 10 mg·kg-1 benzbromarone were intragastricly administered from 5~8 weeks.Serum level of uric acid and XOD activity in liver were tested.Expression of GLUT2 and GLUT5 in intestine was analyzed by immunohistochemistry staining and Western blot.Results Treatment with allopurinol or benzbromarone significantly decreased the serum level of uric acid in fructose-induced hyperuricemic rats.At the same time, allopurinol treatment significantly reduced the XOD activity in liver and GLUT5 expression in intestine.Nevertheless, benzbromarone treatment did not show inhibitory effect on hepatic XOD activity and intestinal GLUT5 expression.In addition, neither allopurinol nor benzbromarone showed inhibitory effect on GLUT2 expression in intestine.Conclusions Allopurinol decreases serum level of uric acid in fructose-induced hyperuricemic rats.The mechanism is related to reducing XOD-mediated uric acid production in liver, and decreasing GLUT5-mediated fructose absorption in intestine.

OBJECTIVE:To study the effect of selenium chitosan on differentiation of HL60 cells and its relationship with the c-myc and c-fos protein expression of cells. METHODS:HL60 cells were divided into 5 groups,i.e. 25 mg?L-1 selenium chitosan group,50 mg?L-1 selenium chitosan group,100 mg?L-1 selenium chitosan group,blank control group and positive control group (dimethy sulfoxide). The differentiation of HL60 cells was measured by NBT-reduced method after administration and amount of positive cells was detected. FCM was used to examine the c-myc and c-fos protein expression of cells. RESULTS:As compared with blank control group,differentiation effect of selenium chitosan groups were similar to that of positive control group; NBT-reduced positive cells were increased while c-myc protein expression of cells was decreased(P

0.1 mV. The rabbits were randomly divided into 4 equal groups of 12 animals in each group: Ⅰ control group in which normal saline 0.2-0.3 ml?kg-1 was injected through epidural catheter; Ⅱ epidural group in which 1% lidocaine 2-3 mg?kg-1 was injected epidurally; Ⅲ propofol group in which propofol was infused iv at 20 mg ? kg-1 ?h-1 for 30 min; Ⅳ combined epidural-propofol group in which epidural 1% lidocaine and propofol iv were both administered. Blood samples were taken before and 4 h, 8 h after ligation for determination of serum concentrations of myocardial troponin I (cTn I ) and MDA and serum SOD activity.The myocardial tissue was taken from the infarct area for determination of the apoptotic myocyte count (TUNEL) and p53 and Bcl-2 protein expressions (LSAB) . Results In control group serum cTn I and MDA increased and SOD decreased significantly after ligation of LAD. Thoracic epidural anesthesia, propofol iv infusion and especially the combination of them suppressed the increase in serum cTn I and MDA and decrease in serum SOD induced by ligation of LAD.In epidural-propofol group and propofol group the apoptotic myocytes count and p53 protein expression significantly decreased, the Bcl-2 protein expression increased as compared with those in control group. Conclusion Thoracic epidural anesthesia combined with propofol can significantly protect myocardium from injury induced by myocardial infarction. This action is mediated by the depression of myocyte apoptosis and the generation of oxygen-derived free radicals.