Objective:To evaluate the clinical effects of the key-way attachment in fixed restoration of mandibular first and second molar deletion.Methods:A 1 -5-year follow-up study was conducted on 1 6 patients with the reconstruction of mandibular first and second molar deletion.The key-way attachment was used as a connector in the fixed partial denture,with the third molar and 2 sec-ond premolars as the abutments.Results:During 5-year follow-up,1 3 of the fixed partial denture were good-looking,stable and functioning.The abutments were stable,without endodontic and periodontal disease.No absorption of alveolar bone was observed by X-ray examination.3 cases were failure.Bleeding on probing of third molar was observed in 1 patient 1 year after restoration.1 re-tainer of a denture on the third molar was loosing 1 .5 years after restoration.1 patient had dental caries on the third molar 5 years af-ter restoration.Conclusion:The key-way attachment as a connector is useful in fixed partial denture in patients with mandibular first and second molar deletion.

Objective To observe effects of soaking therapy with traditional Chinese medicine on athlete's foot. Methods346 cases of the treatment group (175 cases of erosive type and 171 cases of bullous type) were treated with soaking therapy by traditional Chinese medicine and 335 cases of the control group (170 cases of erosive type and 165 cases of bullous type) were treated with 3% Clotrimazolum ointment. Effects of two groups were compared.Results Effect of the treatment group was obviously more superior to that of the control group (P<0.0001). ConclusionsIt shows easy and effective that patients with athlete's foot were treated by soaking therapy with traditional Chinese medicine.

Objective To study the correlations between diopter,axis length,cornea curvature and thickness in myopia patients. Design Prospective case series.Participant 300 myopic patients who were ready for LASIK.Method 300 myopic patients who were ready for LASIK were selected randomly.They were divided into four groups according to the diopter of myopia:low myopia group (≤-3.00D)41 cases,medium myopia group(＞-3.00D,≤-6.00D)130 cases,high myopia group(＞-6.00D,≤-9.00D)95 cases,and super myopia group(＞-9.00D)34 cases.The axis length,cornea thickness,cornea eurvture and diopter of myopia were detected. Through analysis of variance(ANOVA),Pearson correlation analysis and linear regression analysis,the correlations between axis length, cornea thickness,curvature and diopter of myopia were made out in four groups.Main Outcome Measure Diopter of myopia,cornea curvature,axis length of eye and central cornea thickness.Result The axis length of low myopia group,medium myopia group,high myopia group and super myopia group was(24.32?1.73)mm,(25.33?1.49)mm,(26.04?1.92)mm,(27.47?2.57)mm respectively. There were obvious differences of axis lengths between four groups(P=0.000).The cornea curvature in high myopia group was obviously different from those in low myopia group and medium myopia group.There were no significant differences of cornea thickness among four groups(P=0.409).The axis lengths of eyes respectively had correlations with diopters(r=0.734,P=0.000),vertical and horizontal cornea curvatures(r=-0.312,P=0.000;r=-0.328,P=0.000).Conclusion The main effective factor on the diopter of myopia is the axis length for most patients.For the patients with high myopia,the cornea curvature is another effective factor.With the increase of axis length,the cornea curvature decreases,and the cornea turns flatter.(Ophthalmol CHN,2006,15:321-323)

Objective :To study the two common method of internal irradiation for nanometer-iron nuclide,and how to estimation of the absorbed dose for each of them.Methods:We chose the nanometer-iron nuclide emitting β-ray during the decay period,Respectively,Arterial injected into the liver or percutaneous puncture direct inject into the liver.Results:Through the formula,a few nanometer-iron nuclide can produced greater radiation.Conclusion:The estimation of absorbed dose depends on the therapy method.

Carcinoid Tumor ; metabolism ; pathology ; surgery ; Chromogranin A ; metabolism ; Humans ; Male ; Middle Aged ; Orchiectomy ; Teratoma ; metabolism ; pathology ; surgery ; Testicular Neoplasms ; metabolism ; pathology ; surgery

Objective To study P300 wave extraction by a new method. Methods According to the respective characteristics of the brain electrical signal and the signal analysis method, the algorithm by Wavelet Transformation (WT) combined with Independent Component Analysis (ICA), abbreviated to WICA, was applied to P300 wave extraction. Results With WT, the target component of the signal could be strengthened, while the non-target component and the noise were weakened which became weak in multi -channel ICA input signal, and then WICA algorithm could effectively separate strong target component. Conclusion WICA algorithm behaves well in P300 wave extraction.

Objective A prokaryotic expression vector was constructed by inserting the coding sequence of mouse sperm specific lactate dehydrogenase C into pET-28a(+)and the recombinant mLDHC44 protein was purified by Ni+-NTA agrose.Methods The cDNA of mouse sperm specific lactate dehydrogenase C was obtained by RT-PCR,with total RNA of mouse testis tissues as templates.The coding sequence of mouse LDHC4 was amplified by PCR with specific primers.This recombinant vector was transformed into Escherichia coli BL21(DE3).The recombinant mLDHC4 protein was induced by isopropy-?-D-thiogalactoside(IPTG)and identified by sodium dodecyl sulfate polyacrylamide electrophoresis and LDH activity determination.After purified with Ni+-NTA agrose,the mLDHC4 protein was probed with antisera from the pVAX1-mLDHC4 vaccine(the eukaryotic expression vector of mouse sperm specific lactate dehydrogenase C)immunized BALB/c mice by Western blot analysis.Results After digested with BamH I-EcoR I,the recombinant plasmids produced right fragment which was about 1000bp.Sequencing showed that the sequence of the cloned fragment was in agreement with sequence in GenBank.This recombinant vector was named as pET-28a(+)-mLDHC4.With induction of IPTG,The recombinant protein with molecular weight of about 35 kD was expressed and the enzyme activity of this protein was high.After purified with Ni+-NTA agrose,this mLDHC4 protein formed a specific band by sodium dodecyl sulfate polyacrylamide electrophoresis and probed with antisera from immunized BALB/c mice and then formed a specific band in the nitrocellulose membrane.Conclusion The coding sequence of mouse lactate dehydrogenase subunit C had been cloned into the prokaryotic expression vector pET-28a(+)and the mLDHC4 protein could be expressed at a high level,the specificity of this protein was high and the activity was strong.

Objective To investigate the effect of fosinopril on transforming growth factor-?1 (TGF-?1) expression in myocardial tissue of streptozotocin(STZ)-induced diatetic rats. Methods A total of 30 SD rats were enrolled in the study, 10 of them were set as normal controls, others were created the model of diabetes by injecting STZ, and then equally divided into fosinopril group and untreated group. Myocardial TGF-ft was observed in all three groups after 5 weeks. Results The expression of TGF-fi, in myocardial tissue was weakly positive in normal group, strongly positive in untreated group and positive in fosinopriol treat group, with absorbance being 0. 009 ? 0. 004,0. 198 ?0. 007 and 0. 104 ? 0. 005,respectively. Conclusion Fosinopril may partly suppress the increased TGF-?1 expression in myocardial tissue of diatetic rats. It may decrease significantly the myocardial injury of diabetic rats.

Objective To improve the biocompatibility of the metallic stent used for interventional treatment. Methods Inner coatings, outer coatings and micro-porous coatings were made using the solution-injection method, and salt crystallite with demanded size was added in order to develop micro-porous coatings. Results The optical concentrations of polyurethane solution and of salt crystallite (for micro-porous coatings) were obtained, and the corresponding processes were developed. Conclusion The poly-coated stent retains its elasticity, and it become very convenient to develop stents carrying drugs. Its effects on animals ard under test.

Expression of endogenous ouabain in placenta and the concentrations of serum ET-1 and NO were examined in 30 patients with hypertensive disorder complicating pregnancy (HDCP) and 30 healthy pregnant women to investigate the effect of endogenous ouabain on HDCP. Compared with the healthy pregnant group, the expression of endogenous ouabain dramatically increased in the HDCP groups (P<0.01). There was a significantly positive correlation between the expression of endogenous ouabain with ET-1 (r=0.5567, P<0.01), while the correlation of endogenous ouabain and NO was significantly negative (r=-0.6895, P<0.01). As expected, the correlation between ET-1 and NO was negative (r=-0.7796, P<0.01). ET-1 concentrations of maternal and cord sera in HDCP groups were significantly higher in comparison with healthy pregnant group (P<0.01). On the contrast, NO concentrations were much lower in the maternal and cord sera of HDCP groups as compared with healthy pregnant group (P<0.01). Our data suggest that endogenous ouabain is directly involved in the nosogenesis of HDCP, with accompanying decreased NO and the elevated of ET-1.
Case-Control Studies ; Endothelin-1/*blood ; Hypertension, Pregnancy-Induced/*metabolism ; Nitric Oxide/*blood ; Ouabain/*metabolism ; Placenta/*metabolism