Objective To explore potential synergetic effects of interleukin-2(IL-2) with IL-18 on induction of natural killer(NK) cell activity against myeloma cells in vitro.Methods CD69 expression on NK cells were defined by Flow Cytometry;IFN-? level in supernatants of NK cell culture was measured by ELISA;pure NK cell population was obtained from peripheral blood by using cell purification kit;standard Cr51 release method was used to test NK cell cytotoxicity against myeloma cells in vitro.Results The combined application of IL-18 with low dose of IL-2(10u/mL) significantly enhanced NK cell activation,IFN-? secretion and NK cell cytolytic activity against myeloma cells(U266 and RPMI-8226) as compared to IL-18 alone,respectively.Conclusion IL-2 synergized with IL-18 to induce NK cells activates anti-myeloma cell in vitro and combined use of IL-18 and low dose IL-2 could be an alterative immuno-therapy approach for cancers including myeloma.

Objective To explore whether health examination save medical insurance fund or not. Methods To observe the sparing medical cost which 371 health examination people avoid repeated checking and shorten the length of stay during hospitalization from March 2006 to March 2007. To observe the effect of sparing medical cost because of regular health examination, early discovery diseases, pretreatment promptly and avoidance transforming to advanced diseases. Results Because of avoiding repeated checking and shortening the length of stay,everybody save the medical cost about 570 yuan,371 people can save about 21 ten thousands yuan. To statistics the average hospital cost of different courses of eight common diseases and malignant tumors from January to November at 2007, we discovered that the medical cost is large distinction between the earlier period and advanced stage of disease. For example: the medical cost of one stage of hypertension is 6608 yuan ,the two stage is 8838 yuan ,the three stage is 13 869 yuan, and the stage of hypertensive heart disease is 13 033 yuan. Parts of health examination crowd who stay at my hospital from 1996 were visited for five years ,we conformed many chronic diseases by prompt intervention can relieve and even reverse, avoiding the transformation to incurable disease which will consume a large number of strength and money. Conclusion Health examination resource is a medical treatment resource which isn't restricted by social security fund, the utilization can not only save medical cost but also discover and treat diseases earlier, thus we can save the strength and money before the advanced diseases.

This paper reports nursing of 11 patients undergoing laparoscopic radical cystectomy with orthotopic ileal neobladder. The nursing points included preoperative bowel care,psychological care,observation of drainage tubes and complications,guidance of pelvic floor muscle training and bladder training with timed voiding. Patients receiving bladder training after catheter removal,and got self-control urination in 2 weeks. Only a patient left urinary incontinence at night occasionally.

Objective To explore the correlation between the parameters of ambulatory blood pressure monitoring (ABPM) and prostatic volume in elderly patients with essential hypertension.Methods The 131 elderly patients with essential hypertension from March 2007 to December 2009 were enrolled in this study, including 102 benign prostatic hyperplasia (BPH) patients and 29 nonBPH (NBPH) patients. All patients underwent ABPM and ultrasound examination of prostate.Prostatic volumes (PV) were calculated and were compared between BPH group and NBPH group.Correlation between parameters of ABPM and PV was analyzed. Results (1)The maximal mean artery pressure (MAPmax) at night was higher in BPH group than in NBPH group [( 104.7 ± 12. 0)mm Hg vs. (99.6± 10. 1) mm Hg, P= 0.039]. (2)There were correlations between PV and MAPmax in all-day (r=0. 184, P=0. 035), MAPmax at daytime (r=0. 183, P=0. 037), MAPmax at night (r=0. 181, P=0. 040), respectively. Conclusions MAPmax of ABPM may be associated with PV in elderly patients with essential hypertension.

Objective To observe the effects of Shengqing Chinese herbs on the obesity and leptin resistance of diet-induced obesity (DIO) rats.Methods Wistar rats were produced a rat model including of 40 DIO rats and 10 DIO-R rats. The DIO rats were divided into DIO model group, sibutramine group, Jianpi Liqi group and Shengqing group, which were respectively by gavage of saline, sibutramine, Jianpi Liqi herbs (Citri reticulatae pericarpium, Aucklandiae radix, Aurantii fructus immaturus), Shengqing herbs (Bupleuri radix, Cimicifugae rhizoma, Puerariae lobatae radix), blank control group and DIO-R group were by gavage of saline. Their weight, were examined after 16 weeks gavage. Leptin and neuropeptide Y in the serum were examined. Leptin, TNF-α, and suppressors of cytokine signaling-3 (SOCS-3) in the homogenized fat were examined. Results Compared with the DIO-R group, DIO model group's body weight, NPY significantly increased (P<0.01);leptin in the serum and homogenized fat decreased (P<0.05);SOCS-3 increased (P<0.05). After drug intervention, weight of each drug group declined. Compared with the DIO model group, Jianpi Liqi group's leptin in the homogenized fat increased (P<0.05). Shengqing group's leptin in homogenized fat increased, body weight reduced, NPY and SOCS-3 levels decreased, and better than sibutramine group and Jianpi Liqi group (P<0.05,P<0.01).Conclusion Shengqing herbs can reduce obesity and inhibit leptin resistance.

Objective To explore the nutritional condition of primary liver cancer inpatients by means of nutrition risk screening 2002 (NRS2002) and mini nutritional assessment,and compare their feasibility.Methods A total of 80 cases of liver cancer patients hospitalized in Tianjin Third Central Hospital from August 2012 to March 2013 were collected.We investigated the incidence of malnutrition with the scales of NRS2002 and MNA.Comparative analysis of the relationship between the two methods and the traditional single index,liver function was conducted in order to find the more appropriate one for nutritional evaluation.Results NRS2002 got a 33.7％ nutrition risk incidence and MNA showed a 45.0％ risk rate.NRS2002 showed better correlation with traditional individual indicators than MNA.Conclusions Compared with MNA,NRS2002 is more reliable for nutritional assessment in primary liver cancer inpatients.

OBJECTIVE: To prepare Baicalin dispersible tablets and establish a quality control method for this preparation. METHODS: The formula of Baicalin dispersible tablets was optimized by orthogonal experiment with disintegrating time as index. The content and dissolution rate of the tablets were determined by HPLC. RESULTS: The optimized formula was as follows: the contents of crospovidone, sodium starch glycolate and amylum pregelatinisatum were 40%, 6% and 2%, respectively. The dispersible tablets prepared in accordance with the optimize formula could disintegrate completely within 3 minutes and its dissolution rate could reach beyond 90% in 15 minutes. CONCLUSION: The preparation process is simple and feasible, and the prepared Baicalin dispersible tablets had a rapid disintegration and high dissolution rate.

Objective To investigate the antitumor effect of flavopiridol in ovarian cancer. Methods After the treatment with flavopiridol of AO cells,cell apoptotic rate and cell cycle distribution were detected by flow eytometer and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling(TUNEL).Real time PCR was used to detect the expression of cyclin D and active caspase-3 in AO cells.Subcutaneous tumor models and abdominally spread tumor models of human ovarian carcinoma using AO ceils in BALB/c nude mice were established.The mouse survival rates were measured for abdominally spread tumor models and the volume of tumor nodules was determined for subcutaneous tumor models following the treatments of flavopiridol.TUNEL was used to detect cell apoptosis,and immunohistochemistry was used to measure microvessel density(MVD)in tumor tissues. Results AO cells showed apoptotic rates of 4.1%,10.7% and 7.6% following the treatments with flavopiridol at 150,300 and 500 nmol/L respectively,accompanied by an increase in G_1 progression and a decrease in S phase progression.The level of active caspase-3 increased(2.55 vs 2.49)and the level of cyclin D expression decreased significantly(0.25 vs 0.69,P

Objective To study the protective effects of ulinastatin (UTI) on canine islets isolated by automated method and to determine whether the addition of UTI during the in situ pancreatic perfusion process may improve islet recovery. Methods There was no significant difference in donor-related factors (age, gender, warm ischemia time, cold storage time, weight and pancreas weight) between the two experimental groups. Twenty donors were randomly divided into two groups. Pancreases were in situ perfused via abdominal aorta using cold hypertonic citric potassium solution (HC-A) 2 500 ml, group 1 (HC-A group, n =10). The pancreases were perfused with cold HC-A and UTI ( 10 000 U/kg), group 2 (HC-A+UTI group, n =10). Intraductal collagenase V (4℃) delivery with controlled perfusion was done, the pancreas was dissociated in system of Ricordi Chamber and their purified islets were separated with Ficoll density gradient centrifugation. The digestion time, islets remaining trapped in exocrine tissue, final islet purity, insulin and C-pep secretory activity, and islet recovery were observed. The purified islets were observed under light microscopy and electronic microscopy. Results UTI supplementation did not affect the digestion time, islets remaining trapped in exocrine tissue, final islet purify, insulin and C-pep secretory activity and their ultrastructure, and islet recovery was increased. In the HC-A+UTI group the yield of islets was [( 6.17 ? 2.86 )?10 4] IEQ, while in the HC-A group, that was [( 3.42 ? 1.47 )?10 4] IEQ ( P