Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans

Objective　To evaluate the diagnositic value of exercise stress echocardiography (ESE) in patients with pacemaker-dependnet.Methods　Forty-six patients remained pacemaker dependent with a fixed heart rate throughout ESE and coronary angiographic studies.Results　Thirty-four of 46 patients completed the ESE. Coronary artery disease was confirmed in 28 of 34 who had coronary angiograms. ESE had a sensitivity of 75% and specificity of 83% in pacemaker-dependent patients.Conclusions　ESE has preserved diagnostic utility in pacemaker dependent patients with absolute chronotropic incompetence.

Acupuncture ; education ; Acupuncture Points ; Humans ; Meridians

Acupuncture Points ; Humans

Objective To evaluate the use of bunching forceps during radical prostatectomy. Methods 14 cases of radical prostatectomy/cystectomy were performed with the use of bunching forceps to control bleeding from the dorsal vein complex. Results The procedure resulted in minimal bleeding from dorsal complex and was helpful in rhabdosphincter. Conclusions The bunching forceps is very valuable for controlling bleeding from dorsal vein complex and for preserving rhabdosphincter.

Objective To investigate clinical efficacy and safety of carboprost tromethamine injection combined with low B -Lynch suture for intractable postpartum hemorrhage placenta previa.Methods 125 cases of intractable postpartum hemorrhage placenta previa were divided into two groups according to treatment(n =62)with the observation group(n =63),the control group was administered oxytocin,and the line in the placental separation surface 8 interrupted suture;the observation group was used carboprost tromethamine injection combined with low B -Lynch suture.Clinical efficacy,blood loss,bleeding,postoperative bleeding 2h,24h after blood loss,transfusion rate and index sex hormone levels were compared before and after treatment.Results (1)After treatment,total effec-tive rate of the control group and observation group was 77.42%(48 /62)and 95.24%(60 /63)respectively.There was a statistically significant difference between the two groups(P <0.05);(2)Blood loss,bleeding time,2h after blood loss,blood loss and transfusion rate after 24h in the observation group were (1 608.37 ±43.01)mL,(8.89 ± 1.09)min,(101.18 ±8.73)mL,(221.17 ±20.58)mL and 4.76%,respectively,which were significantly lower than those of the control group [(1 992.28 ±55.62)mL,(12.83 ±1.77)min,(145.49 ±12.19)mL,(255.54 ± 23.30)mL and 16.13%](t =4.559,5.128,3.928,3.382,χ2 =5.559,all P <0.05);(3)before and after treatment indicator hormone(E2 ,FSH and LH)levels were not significantly different(P >0.05),and after treatment,the index level differences were not statistically significant(P >0.05);(4)the two groups during treatment,liver and kidney functions were normal,three cases of the control group and two cases of the observation group occured nausea and vomiting.Conclusion Carboprost tromethamine injection combined with low B -Lynch suture has significant effect, a small amount of bleeding,high security for intractable postpartum hemorrhage placenta previa,which should be promoted.

Objective The gene encoding part of HTLV(Human Tcell leukemia viruses) envelope protein gp21 was cloned and expressed in order to prepare diagnostic agent for detecting HTLV.Methods The gene encoding part of HTLV envelope protein gp21 was obtained by combining four oligonucleotides into a long DNA fragment,then cloned and subcloned into Tvector and pGEX2T respectively.Expression was performed under induction in a fusion way that target protein gene was inserted downstream the carrying protein gene of pGEX2T which makes purification of target protein convenient.At last,Target protein was purified by affinity chromatography in glutathione sepharose 4B column and its antigenicity was confirmed by immunoblotting.Results and conclusion Sequence analysis showed it was feasible to obtain a target gene by ligating short oligonucleotides;As expected,target protein was expressed which exhibited strong antigenicity after being subcloned into fusion expression vector pGEX2T.

Objective To explore the effects of basic fibroblast growth factor(bFGF) pre-inducing on marrow stroma cells(MSCs) which were induced into dopaminergic neurons.Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats.MSCs were cultured,proliferated and purified by passage culture.After being induced by bFGF,cultuered MSCs were divided into experimental groups(GM1 group,GDNF group and GDNF+ GM1 group) and control group.The surface markers of the differentiated neuron,such as neurone specific enolase(NSE),glial fibrillary acidic protein(GFAP) and TH were detected by immunocytochemistry after MSCs were cultured in induction media for 3 days and 7 days.Results In control group,the NSE expression of MSCs was very low.Many NSE-positive cells and TH-positive cells were found in the experimental groups at 3 or 7 days after induction.The percentage of NSE-positive and TH-positive cells of GDNF+GM1 group was significantly more than the other experimental groups(all P