1.Preparation of transferrin and RGD co-modified PLGA nanoparticles and its targeting to melanoma
Chinese Journal of Biochemical Pharmaceutics 2014;37(4):19-21
Objective To prepare transferrin and Arg-Gly-Asp polypeptide co-modified nanoparticles(TF/RGD-NPs)and evaluate its targeting efficiency to melanoma.Methods The co-modified nanoparticles were prepared by emulsion method and its appearance,particle size and Zeta potential were evaluated.The cellular uptake experiment and melanoma tumor spheroids penetration test were used to evaluate the affinity and ability to penetrate tumor tissues of TF/RGD-NPs to melanoma B16 cells. Results The particle diameter of co-modified nanoparticles was(113.4 ±12.5)nm and the Zeta potential was(4.53 ±2.15)mV.In vitro uptake test demonstrated that the efficacy of cellular uptaken TF/RGD-NPs by B16 cells were 2.7 times and 2.9 times to TF-NPs and RGD-NPs,respectively,the differences were all significant(P<0.05 ).Tumor spheroid penetration test results showed that TF/RGD-NPs has good affinity to melanoma cells.Conclusion TF/RGD-NPs can target to melanoma B16 cell efficiency in vitro,it may be serve as a potential drug delivery system for targeting melanoma.
2.Effect of embelin and doxorubicin on the proliferation of MCF-7 cell and growth of MCF-7 bearing nude mice
Chinese Journal of Biochemical Pharmaceutics 2014;37(4):5-7,11
Objective To explore the effect of X-linked inhibitor of apoptosis protein(XIAP)combined with doxorubicin on the proliferation of MCF-7 cell and growth of MCF-7 bearing nude mice.Methods The anti-proliferation efficiency of doxorubicin and embelin were determined by MTT assay.MCF-7 cell apoptosis induced by embelin and doxorubicin were detected by Flow cytometry.Anti-tumor ability of embelin and doxorubicin were evalued with tumor spheroids test.MCF-7 cell were xenografted to mice to establish the animal model,which was used to evaluate the effect of anti-tumor. Results Compared with saline group,embelin group and doxorubicin group,the combination(doxorubicin +embelin)group inhibited the growth of MCF-7 cells effectively(P<0.01).The combination group induced the apoptosis of MCF-7 cells more effectively than doxorubicin alone(P<0.01), and significantly inhibit the growth of tumor in vitro and in vivo than other groups(P<0.01).Conclusion The combination of doxorubicin and embelin may be used as a potentially effective treatment method for breast cancer.
3.Clinical observation of chemotherapy combined with yiqi xiaozheng granule in treating advanced malignant tumor.
Hong SUN ; Ping-ping LI ; Yan-zhi CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(5):382-383
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
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Cisplatin
;
administration & dosage
;
Drug Therapy, Combination
;
Drugs, Chinese Herbal
;
therapeutic use
;
Female
;
Fluorouracil
;
administration & dosage
;
Humans
;
Intestinal Neoplasms
;
drug therapy
;
Lung Neoplasms
;
drug therapy
;
Male
;
Phytotherapy
;
Tetrahydrofolates
;
administration & dosage
4.Desmoplastic small round cell tumor of ovarian: report of a case.
Ping-ping SUN ; Ji LI ; Zhi-yong LIANG
Chinese Journal of Pathology 2010;39(3):197-198
12E7 Antigen
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Adult
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Antigens, CD
;
metabolism
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Cell Adhesion Molecules
;
metabolism
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Desmin
;
metabolism
;
Desmoplastic Small Round Cell Tumor
;
metabolism
;
pathology
;
surgery
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Diagnosis, Differential
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Female
;
Humans
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Neoplasm Recurrence, Local
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Neuroblastoma
;
metabolism
;
pathology
;
Ovarian Neoplasms
;
metabolism
;
pathology
;
surgery
;
Phosphopyruvate Hydratase
;
metabolism
;
Rhabdomyosarcoma, Embryonal
;
metabolism
;
pathology
;
Sarcoma, Ewing
;
metabolism
;
pathology
;
Vimentin
;
metabolism
5.Biofeedback therapy for myogenic fecal incontinence
Daqing SUN ; Ping ZHAO ; Yuli CHEN ; Jinliang LI ; Ping ZHANG
Chinese Journal of General Surgery 2001;0(07):-
Objective To evaluate the effect of biofeedback therapy for the treatment of myogenic fecal incontinence. MethodsA general assessment about anorectal function was made on 17 cases receiving biofeedback therapy including muscle power training,sensory training and coordination training. Results The clinical scores before and after biofeedback therapy were 1.66?0.23,3.80?0.42 respectively,with an effective rate of 82%. The anus maximum contracting pressure elevated,(73?20) mm Hg vs. (123?30) mm Hg; myoelectric amplitude increased,(122?32) ?V vs. (230?41) ?V;Contracting time prolonged,(4.1?2.0) s vs. (9.4?3.0) s; The sensory threshold was lowered,(50?12) ml vs. ( 20? 10) ml;The feel-contract time increased,(3.1?0.4) s vs. (1.2?0.3) s. Positive rectal contraction reflex was seen in 41% patients before therapy compared with 82% after therapy. Conclusions Biofeedback therapy increases contractility of sphincter,decreases threshold of rectal sensory,and is a therapy of choice for myogenic fecal incontinence.
6.Successful treatment of paraquat poisoning with hemolytic anemia: a report of 1 case.
Bao-quan SUN ; Bo ZHAO ; Ping LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(8):624-624
Anemia, Hemolytic
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chemically induced
;
therapy
;
Female
;
Humans
;
Paraquat
;
poisoning
;
Poisoning
;
therapy
;
Young Adult
7.Expert's Comment.
Chinese Journal of Pediatrics 2011;49(8):616-617
Female
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Humans
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Male
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Sepsis
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complications
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Wernicke Encephalopathy
;
diagnosis
9.Influence of different P_(ET)CO_2 on the quantitative EEG and auditory evoked potential hides during inhalation anesthesia
Yongxing SUN ; Xuesong ZHANG ; Ping LI
Chinese Journal of Anesthesiology 1995;0(12):-
Objective To investigate the effect of different end-tidal PCO2 (PETCO2) on the quantitative electro-encephalogram (qEEG) and auditory evoked potential index (AAI) in patient undergoing elective general surgery under inhalation anesthesia. Methods Thirty-eight ASA Ⅰ-Ⅱ patients aged 22-48 yrs weighing 49-79 kg undergoing elective general surgery under inhalation anesthesia were studied. The intra-operative blood-loss was expected to be
10.Analysis of Correlative Factors of Neonatal Stress Ulcer Prognosis
jing, LI ; jian-hua, SUN ; ping, HUANG
Journal of Applied Clinical Pediatrics 2006;0(18):-
Objective To analyze the correlative factors of prognosis of neonatal stress-induced ulcer bleeding.Methods Fifty-four cases of NICU admitted stress-induced ulcer bleeding newborns were divided into mild group and severe group according to bleeding endurance,transfusion requirement,and clinical turnover.Compared with gender,gestational age,birth weight,and score for neonatal acute physiology(SNAP) Ⅱ and score for neonatal acute physiologyⅡ perinatal extension(SNAP-PE) Ⅱ of two groups.Results Based on the output of SPSS statistical package,the SNAP Ⅱand SNAP-PE Ⅱ had a significant deviation between two subgroups((?~2=)15.207,10.311 all P