1.A method of human serum folic acid dectetion by non-equilibrium competitive immunoassay using FITC detecting system
Hong NIE ; Weixian CHEN ; Qin ZHAO ; Ding WANG ; Qin HU ; Ping LIU ; Pu LI
Chongqing Medicine 2017;46(6):792-795
Objective To prepare anti-folic acid (FA) polyclonal antibody and develop a new non-balanced competing chemiluminescence analysis for clinical detection of FA.Methods Established the detection method by added FITC-FA-analogs and FAHRP-antibody in the light emitting plate,which coated with anti-FITC antibody,to form the immune response complex of FITC/antibody-FITC-FA-analogs/FA-antibody-HRP.Then methodology evaluation was performed to evaluate the method performance;and further compared the detecting results with non-FITC system detection system and Electrochemiluminescence system (Roche Elecsys 2010).Results The ELISA results showed that the prepared anti-FA antibodies can recognize serum FA specificly.The methodology evaluation indicated that the linear correlation coefficient of the standard curve was 0.990 0;the analytical sensitivity was 1.21 ng/mL;the range of linear detection was 1.21~ 38.80 ng/mL;The coefficient variability of intra-assay was <5 %,which was better than the results of non-FITC detection system;and the correlation coefficient was 0.908 1 compared with the Elecsys-2010 detection system.Conclusion The established chemiluminescence immunoassay for human serum FA has a good sensitivity and specificity,and suitable for clinical serum FA quantitativedetecting.
2.Development of the mouse spinal cord and neuroapoptosis
Juan DENG ; Hong ZHENG ; Xue LI ; Shuai XUE ; Lili LI ; Mengyue NIE ; Ping WU ; Jinbo DENG
Acta Anatomica Sinica 2014;(4):457-464
Objective To investigate the neural proliferation , differentiation and apoptosis of the developing spinal cord of the mouse and to discuss the mechanism of spinal cord ’ s development .Methods 5-Bromodeoxyuridine ( BrdU) assay was used to mark the proliferative neural stem cells , and the immunofluorescent stainings ( DCX, NeuN and Caspase8) were carried out to visualize the newborn neurons , mature cells and apoptotic cells in the spinal cord with 173 mice arrange from E18 to P90.Results BrdU positive neural stem cells appeared evenly in the spinal cord at early days . With age increasing , the neural stem cells differentiated into neuroglial cells and neurons .The newborn neurons in the subventricular zone migrated toward the intermediate zone ( putative gray matter ) and differentiated into mature neurons gradually .With neurons ’ concentrating towards the center , the gray matter formed an “H” shape .In the meantime , with neural differentiation , some apoptotic neurons appeared among the newborn neurons and mature neurons . Double immunostaining showed that most apoptotic neurons were newborn neurons , suggesting the neuroapoptosis more likely occurred in newborn neurons .The statistical data showed that the number of DCX , NeuN and Caspase-8 positive cells reduced with age increasing , suggesting neural differentiation and neuroapoptosis decreased during spinal cord ’ s development .Conclusion Neural proliferation , neural differentiation and neuroapoptosis occur in developing spinal cord . They work together to regulate the formation and development of the spinal cord .
3.Atorvastatin reduces the expression of COX-2 mRNA in peripheral blood monocytes in patients with acute myocardial infarction and modulates the early inflammatory response.
Ping DENG ; Shui-ping ZHAO ; Jie WU ; Shao-cai HONG ; Zhi-hong WU ; Hong-nian ZHOU ; Sai NIE
Chinese Journal of Cardiology 2005;33(11):1018-1022
OBJECTIVETo measure the effect of atorvastatin on COX-2 expression in monocytes in patients with acute myocardial infarction (AMI).
METHODSForty patients with AMI (AMI group) and 18 patients with stable coronary heart disease (control group) were enrolled, and patients with AMI were randomly given routine therapy (n = 20) and routine therapy plus atorvastatin (20 mg/day, n = 20) for a week. Peripheral blood monocytes for each participant including patients with AMI were isolated and cultured for 24 hours. During the culture, monocytes in patients with pretreatment AMI were incubated with celecoxib in different concentration (0, 0.1, 1 and 10 micromol/L). COX-2 mRNA expression in monocytes was measured by reverse transcription polymerase chain reaction (RT-PCR); concentrations of interleukin-6 (IL-6) in supernatant from monocytes and plasma hs-CRP levels were measured by using enzyme-linked immunosorbent assay (ELISA).
RESULTSCOX-2 expression in monocytes in patients with AMI (0.92 +/- 0.13) was significantly higher than that in the control subjects (0.19 +/- 0.08), and decreased by 66% after atorvastatin (compared with that on routine therapy, P < 0.05); IL-6 secretions of monocytes in the AMI group (204.8 +/- 45.6 ng/L) increased dramatically compared with those in the control group (40.9 +/- 1.2 ng/L, P < 0.05), and reduced dramatically by 58% when incubated with 10 micromol/L celecoxib (P < 0.05) in a concentration-dependent manner; plasma levels of CRP in the AMI group (43.3 +/- 14.9 mg/L) significantly increased compared with those in the control group (1.7 +/- 0.8 mg/L), and reduced by 62% after atorvastatin (compared with those in the routine therapy group, P < 0.05). COX-2 expression in monocytes in the AMI group was positively correlated with both secretions of IL-6 and plasma level of CRP (r = 0.636 and 0.662, respectively, both P < 0.05).
CONCLUSIONSThere is an inflammatory activation in peripheral blood monocytes in patients with early AMI, and the monocytes-derived COX-2 may play an important role in promoting early inflammatory process. Atorvastatin may decrease COX-2 expression in peripheral blood monocytes in patients with AMI and cyclooxygenase-dependent pathway might be correlated with the anti-inflammation mechanism of statin.
Aged ; Atorvastatin Calcium ; Cyclooxygenase 2 ; metabolism ; Female ; Heptanoic Acids ; therapeutic use ; Humans ; Inflammation ; Interleukin-6 ; metabolism ; Leukocytes, Mononuclear ; drug effects ; metabolism ; Male ; Middle Aged ; Myocardial Infarction ; drug therapy ; metabolism ; Pyrroles ; therapeutic use ; RNA, Messenger ; genetics
4.Preliminary Study on a Potential Panel for Quality Assurance of ELISPOT
Chuntao ZHANG ; Yu WU ; Chenyan ZHAO ; Kunxue HONG ; Chunyu LIU ; Ying WANG ; Ping ZHONG ; Jianhui NIE ; Xuelin WU ; Youchun WANG
Virologica Sinica 2008;23(5):330-338
The ELISPOT assay is increasingly used for assessing cellular immune responses in clinical trials of HIV-1 or cancer vaccines. However, to date, data from clinical trials do not consistently show that immune responses are correlated with clinical endpoints. This is due in part to the lack of assay standardization and validation across laboratories and therefore, a quality control panel is required to establish competency and comparability amongst different laboratories. In this study peripheral blood mononuclear cells (PBMCs) from healthy individuals were screened and frozen in liquid nitrogen. The recovery and viability of the PBMCs and the frequencies of interferon (IFN)-γ-secreting cells after CEF peptide pool stimulation were detected after various intervals in seven different laboratories. The recovery and viability did not differ significantly after different intervals. Although the frequencies of IFN (interferon)-γ-secreting cells among thawed PBMCs (peripheral blood mononuclear cells) fluctuated after CEF peptide pool stimulation at different intervals, they were not significantly decreased compared with those among fresh PBMCs. However, the viabilities, recoveries and frequencies of IFN-γ-secreting cells differed significantly among the seven laboratories. Our results indicate that cryopreserved PBMCs could be used as a quality control panel for ELISPOT. However, the procedures for ELISPOT need to be standardized amongst different laboratories.
5.Semiconductor low level laser irradiation for exposure of hydroxyapatite orbital implants.
Hong-fei LIAO ; Qiang-juan CHEN ; Jing-lin YI ; Zhen FENG ; Xiang-rong ZHANG ; Ping-ping NIE
Chinese Journal of Plastic Surgery 2004;20(3):177-179
OBJECTIVETo evaluate the efficacy of semiconductor low level laser irradiation for the treatment of postoperative exposure of hydroxyapatite orbital implants.
METHODS22 cases with postoperative exposure of hydroxyapatite orbital implants were divided into three groups according to the size of implants exposure. The exposure wound in the 3 groups was irradated with semiconductor low level laser 5 min per day for 5-15 days. The follow-up period ranged from 2 to 24 months.
RESULTSIn the group with less then 3 mm of exposure, the wound healed in 1 week after 5-10 days irradiation; in the group with implant exposure of 4-7 mm, the would healed in 1-2 weeks after 10-15 days irradiation; in the group with implant exposure of 8-10 mm, the would healed in 2-3 weeks after 10-15 days irradiation. Compared with the treatments of drugs and/or surgical repair, which was used for another 20 cases of exposure of hydroxyapatite orbital implants, semiconductor low level laser increased healing rate obviously in the groups with implant exposure of 4-7 mm and 8-10 mm (P = 0.019, 0.018).
CONCLUSIONSemiconductor low level laser has better effects than drugs and/or surgical repair for exposure of hydroxyapatite orbital implants.
Adolescent ; Adult ; Aged ; Child ; Durapatite ; therapeutic use ; Eye ; pathology ; radiation effects ; Female ; Follow-Up Studies ; Humans ; Low-Level Light Therapy ; methods ; Male ; Middle Aged ; Orbital Implants ; adverse effects ; Postoperative Complications ; etiology ; radiotherapy ; Semiconductors ; Treatment Outcome
6.Chemotherapy and radiotherapy for non-Hodgkin's lymphomas of the nasal cavity.
Da-hong NIE ; Wei-han HU ; Yuan-hong GAO ; Yong WU ; Shu-ping ZHANG
Chinese Journal of Oncology 2004;26(5):312-314
OBJECTIVETo investigate the effects of different treatments on the prognosis of patients with non-Hodgkin's lymphomas of the nasal cavity.
METHODSA retrospective study of 59 patients who suffered from stage I(E) primary non-Hodgkin's lymphomas of the nasal cavity was presented. They were treated by radiotherapy and chemotherapy of CHOP regimen, in which 33 patients received chemotherapy plus radiotherapy, 8 patients received radiotherapy plus chemotherapy, 10 patients received chemotherapy alone, and 8 patients received radiotherapy alone. Survival analysis was performed by Kaplan-Meier method, the difference between groups was evaluated by log-rank test, and the comparison of rates was carried out by chi(2) test.
RESULTSThe overall 1-, 3- and 5-year survival rates were 71.2%, 42.0% and 38.5%, respectively. There was no significant difference among the patients received different treatments (chi(2) = 2.98, P = 0.3943), but the patients received radiotherapy plus chemotherapy seemed to have a better survival curve than other patients. The 1-, 3- and 5-year survival rates were 84.2%, 67.7% and 62.0% for lesion limited in nasal cavity but 50.0%, 14.3% and 14.3% for lesion extended and involved the adjacent structures (chi(2) = 10.46, P = 0.0012). As the initial therapy, 24 patients who received chemotherapy of more than 3 cycles, and 16 patients who received radiotherapy of more than 40 Gy, and the complete response (CR) rates were 25.0% and 75.0% (chi(2) = 9.697, P = 0.002). Among 43 patients received chemotherapy, the CR rates for those who received 2, 3 - 4 and 5 - 6 cycles were 10.5%, 25.0% and 25.0%, respectively (chi(2) = 1.467, P = 0.48). Patients who received chemotherapy plus radiotherapy have higher rates of both complication and treatment-related mortality, but the difference was not statistically significant (P = 0.202 and 0.693).
CONCLUSIONFor stage I non-Hodgkin's lymphomas of the nasal cavity, radiotherapy should be the first treatment to get early local control. Chemotherapy may be followed at the discretion of the pathological grade and clinical staging, or IPI.
Adolescent ; Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; therapeutic use ; Child ; Child, Preschool ; Cobalt Radioisotopes ; Combined Modality Therapy ; Cyclophosphamide ; administration & dosage ; Doxorubicin ; administration & dosage ; Female ; Humans ; Lymphoma, Non-Hodgkin ; drug therapy ; radiotherapy ; Male ; Middle Aged ; Nasal Cavity ; Nose Neoplasms ; drug therapy ; radiotherapy ; Prednisone ; administration & dosage ; Prognosis ; Retrospective Studies ; Survival Analysis ; Vincristine ; administration & dosage
7.Performance of Automatic Blood Cell Analysis Parameters for Differential Diagnosis of Myelodysplastic Syndromes and Aplastic Anemia
Qun NIE ; cheng Zhuo CHEN ; ping Li NIE ; Yu ZHOU ; yu Hong ZHANG ; xin Jian LI
Journal of Modern Laboratory Medicine 2017;32(5):74-77,82
Objective To evaluate the performance of automated blood cell analysis parameters for differential diagnosis of myelodysplastic syndromes (MDS) and aplastic anemia (AA).Methods Data of automatic blood cell analysis parameters at diagnosis of confirmed patients with MDS and AA from December 2002 to February 2011 in Peking University Shenzhen Hospital were retrospectively reviewed.Results 33 cases of MDS and 36 cases of AA were recruited in this study.Based on the evaluable data,mean corpuscular hemoglobin concentration (MCHC) (328.58 ± 17.24 g/L vs 342.47±18.75 g/L,n=33/36) was significantly lower (P=0.002 1),while monocyte percentage (MONO%) (11.48±9.99 vs 6.94±2.50,n=32/34),platelet distribution width (PDW%) (13.51±4.24 vs 10.62±3.68,n=20/22) and platelet hematocrit (PCT%)(0.11 ±0.10 vs 0.04±0.07,n=11/15) were markedly higer (all P<0.05) in patients with MDS than that of AA.No significantly differences for other blood cell analysis parameters were seen between patients with MDS and AA.Under the condition of best cut-off value,areas under the ROC curve of MCHC,MONO%,PDW and PCT were 0.706 (95% confidence interval:0.584~0.809),0.666 (0.540~0.778),0.668 (0.506~0.805) and 0.745 (0.538~0.894) respectively.MONO% and MCHC had high specificities (97.06% and 88.89%) and positive predictive values (93.3% and 80.0%) for differential diagnosis of MDS from AA.Conclusion MONO% and MCHC may be used as simple indicators for differential diagnosis of MDS and AA.
8.Relationship between CYP1A1 gene polymorphisms and urinary 1-hydroxypyrene levels in coke oven workers.
Ji-sheng NIE ; Hong-mei ZHANG ; Jian-ya SUN ; Ping ZENG ; Ling ZHANG ; Qiao NIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(5):270-275
OBJECTIVETo study the associations of CYP1A1 gene polymorphisms with levels of urinary 1-hydroxypyrene among coke oven workers.
METHODS223 male workers from a coke plant (76, 82 and 65 workers in oven top group, oven-side group and oven-bottom group respectively) and 119 controls without occupational polycyclic aromatic hydrocarbons exposure were selected. The MspI gene polymorphism in CYP1A1 3' flanking region and the genotypes at I462V site in exon 7 of CYP1A1 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific amplification (ASA).
RESULTSThe urinary 1-hydroxypyrene of coke oven workers in oven-top, oven-side and oven-bottom (3.77+/-0.64, 3.57+/-0.49, 3.26+/-0.80 micromol/mol Cr) were significantly higher than controls (2.80+/-1.02 micromol/mol Cr) (P<0.01). The urinary 1-hydroxypyrene was not significantly different among MspI genotypes in CYP1A1 3' flanking region (P>0.05). In oven-top group and oven-side group, the subjects with Val/Val genotype in exon 7 of CYP1A1 had significantly higher urinary 1-hydroxypyrene levels than those with Ile/Val or Ile/Ile genotype, and urinary 1-hydroxypyrene of Ile-Val genotype were also significantly higher than Ile/Ile genotype (P<0.01). Multivariate logistic regression analysis showed that the coke oven workers (OR in oven top group, oven-side group and oven-bottom group was 24.926, 4.226 and 6.729 respectively) and subjects with m2/m2 genotype in CYP1A1 3' flanking region (OR=4.031) or with Val/Val or Ile/Val genotype in exon 7 of CYP1A1 (OR were 5.524 and 3.811) had elevated urinary 1-hydroxypyrene (greater than 95 percentile of control group, 3.876 micromol/mol Cr).
CONCLUSIONBAP concentration of work environment contributes to the elevated urinary 1-hydroxypyrene levels, and the exposed BAP levels were regulated by the CYP1A1 MspI and I462V genotypes. Genetic polymorphism of CYP1A1 gene could be a susceptible biomarker in coke oven workers which was involved in the individual susceptibility on metabolism of PAHs.
Adult ; Benzo(a)pyrene ; adverse effects ; Coke ; Cytochrome P-450 CYP1A1 ; genetics ; Humans ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Polymorphism, Genetic ; Pyrenes ; pharmacokinetics ; Urine ; chemistry
9.Regional genotyping and epidemiological characteristics regarding Yersinia pestis isolates in Shaanxi Province, China
hong Cui AN ; bao Bao CHEN ; Wen LYU ; min Shou NIE ; ping Suo FAN ; xin Yang SUN
Chinese Journal of Zoonoses 2017;33(10):916-919
We typed Yersinia pestis isolated from plague foci of Shaanxi Province using different region (DFR) and analyzed epidemiological characteristics.Twenty-three DFRs primers and PMT1 (plasmid) primer were used to verify the DFR genomovars and 48 Yersinia pestis were involved to analyze DFR profiles and epidemiological characteristics.In the same year,the genotypes of Yersinia pestis isolated from different infected vector and animals were basically the same.Three genomovars named Genomovar 11,17,and 20 were verified in 48 Yersinia pestis strains in Shaanxi Province.The main genotypes were different in different epidemic years.In 1987-1988 and 2000-2001 years,genomovar 17 was major genomovar and genomovar 20 in 2006 year.In conclusion,the dominant genotypes were different in different epidemic years.As time goes on,DFR genomovars of Yersinia pestis undergone the evolution of gene deletion,which changes genomovar 17 into genomovar 20.
10.Retrospective analysis for cancer screening among 898 elderly male patients
Xin NIE ; Ping ZHANG ; Yongqiang ZHANG ; Hong SHI ; Lin LI
Chinese Journal of Geriatrics 2019;38(4):409-411
Objective To investigate the distribution,treatment and prognosis of cancer among elderly male patients.Methods Clinical data of 898 male subjects aged 60-105 years with a median age of 82 years who participated in cancer screening in gerontology center of Beijing Hospital from January 1995 to January 2015 were collected in this retrospective study.Low-dose computed tomography,gastroenterological endoscope,alpha fetoprotein plus abdominal ultrasonography,and prostate specific antigen were used to screen lung cancer,gastroenteric cancer,liver cancer,and prostate cancer respectively.The incidences of malignant tumors,treatments and efficacies were collected during the study period.Results The 136 cases(85.0%)were diagnosed as malignant tumors by screening and 24 cases(15.0 %)by clinical symptoms.Three of the most common cancers were lung cancer(46 cases,28.8 %),prostate cancer(33 cases,20.6 %),and colorectal cancer(15 cases,9.4 %).The median age of cancer diagnosis was 73 years.The prevalence of cancer was higher in males aged over 70 years than in males aged under 70 years(18.1% or 112/620 vs.7.2%,20/278,P < 0.01).The median survival time after cancer diagnosis was 5 years (range of 1-20 years).Compared with patients diagnosed by clinical symptoms,patients diagnosed by screening had higher early detection rate of cancer(97.8% or 133/136 vs.54.2% or 13/24,P <0.01),higher definitive therapy rate(79.4% or 108/136 vs.58.3% or 14/24,P<0.05),and lower recurrence rate(6.5% or 7/108 vs.28.6% or 4/14,P<0.01).Conclusions The positive rate of cancer screening is high in males over 70 years old.Elderly males could benefit from early diagnosis and treatment by cancer screening.