Cases teaching can cultivate students sharper awareness of marketable thinking,and improve students skills in dealing with cases.The keys to guarantee the teaching quality are teachers,wise selection of cases and the flexible delivery in classroom.

Objective To explore the clinical efficacy of intensive pulsed light(IPL) on body hair removal.(Methods A) total of 172 patients who suffered from heavy body hairs were treated by IPL since April 2002.It took 3 to 5 procedures,with the interval of one month. Results After treatment,80 patients(47%) were cured,42(24%) improved,and 50(29%) had no effect. Conclusion The intensive pulsed light system is safe and efficient in hair removal.

Objective To analyse the MRI characteristics of the chronic clicking temporomandibular joints(TMJs).Methods 20 TMJs in 10 young chronic clicking individuals (12 clicking TMJs)and 60 TMJs in 30 young healthy volunteers were examined by MRI scanner, and sagittal oblique opened-mouth and closed-mouth position images were observed.Then comparing the MRI findings of the acquired j oints between two groups.Results The ratio of the non-double concave articular disc,slightly high signal intensity in the articular disc, abnormal changes of the condyle,articular fluid,anterior displacement of the articular disc,and increasedDraceangles in the clicking group were significantly higher than those in the control group(P<0.05).The result revealed statistically significant differences.Conclusion The MRI anatomical characteristics can be an indicator for the TMJ anomalys,and provide an obj ective radiological basis for clinical diagnosis and treatment to some extent.

Objective To observe the effect of Tongmai Oral Liquid(TOL) on renal pathological feature and tumor growth factor ?1(TGF-?1) in rats with experimental diabetic nephropathy(DN).Methods Forty SD male rats were used for the experiment.Of which,8 were in the normal group,and the other rats were given one-dose intraperitoneal injection of streptozocin(STZ),and were fed with high-fat forage every other day.The normal group received one-dose intraperitoneal injection of aseptic citric acid-sodium citrate buffer and routine forage.After feeding for 4 weeks,the rat models of DN were randomized into the model group and TOL group,which were given normal saline and TOL(2.5 g?kg-1?d-1) respectively,and the two groups were still fed with high-fat forage.After treatment for 6 weeks,the rats were executed and the pathological features and ultrastructure of renal tissue were observed under light microscope and electron microscope respectively.Mean glomerular area(MGA),mean extracellular matrix area(MMA),and the thickness of glomerular basement membrane(GBM) as well as the ratio of MMA/MGA were measured.Meanwhile,the expression of tumor growth factor ?1(TGF-?1) was detected by immunohistochemical method.Results In TOL group,the pathological features such as broadened mesentery,thick basement membrane and vacuolar degeneration in the renal tubular epithelium were relieved,MMA/MGA ratio was decreased,GBM became thin,and the expression of TGF-?1 was strong mainly in renal tubular and interstitial region while weak in glomeruli,and its area density and integral optimal density were decreased as compared with the model group(P

Menopause is inevitable physiological process of women in the process,per-imenopause syndrome is a common physical and mental diseases in middle-aged women. However,most of the middle-aged women lack health knowledge and are prone to depression,anxiety, paranoia,and other psychological problems. This has seriously affected daily lives and work. Nurses should give perimenopausal syndrome patients mental health different care and education.

Objective To evaluate the effect of ultrasonic speckle technique on cardiac function in patients with acute myocardial infarction (AMI).Methods 30 patients with AMI were selected as observation group,30 healthy people were selected as control group,and they were all detected by ultrasonic speckle technique.The differences of measurement indices of cardiac function with ultrasonic speckle technique were compared between the observation group and control group.The difference between ultrasonic speckle technique and its measured value was observed with single photon emission computed tomography(SPECT) as the gold standard.The correlation between the results of three-dimensional speckle ultrasound and SPECT in patients with AMI was analyzed.Results The parameters of LVEDV [(62.2 ± 6.4) mL vs.(80.3 ± 7.5) mL],LVESV [(21.7 ± 5.6) mL vs.(35.4 ± 7.2) mL] and LVEF [(62.6 ± 5.9)％ vs.(72.8 ± 5.6)％] in the observation group were significantly lower than those in the control group,the IVSTd [(1.1 ± 0.2) cm vs.(0.9 ± 0.2) cm] and LVPWd [(1.2 ± 0.2) cm vs.(0.8 ± 0.1) cm] were significantly higher than those in the control group,the differences were statistically significant(t =8.431,9.865,3.651,8.798,6.654,all P ＜ 0.05).In AMI patients,there were no significant differences in left ventricular parameters LVEDV,LVESV,LVEF measured between ultrasonic speckle technique and SPECT(all P ＞ 0.05).With SPECT as the gold standard,in AMI patients,the ultrasound parameters LVEDV,LVESV and LVEF were positively correlated with SPECT parameters(r =0.912,0.903,0.899,all P ＜ 0.05).Conclusion Ultrasound speckle technique can well evaluate myocardial function in patients with myocardial infarction.

Objective To explore the relation between serum Hcy,Cys C levels and type2 diabetic macro-vascular complications and its clinical significance.Methods The serum levels of Hcy and Cys C were measured with immunoturbidimetry in 80 cases of type 2 diabetic patients with macrovascular complications and 80 cases of type 2 diabetes without macrovascular complications,and in 80 healthy subjects which was seleted as control.Results Serum levels of Hcy and Cys C of type 2 diabetic patients with macrovascular complications were (26.42 ± 5.75)μmol/L and (2.30 ±0.40)μmol/L,which were higher than those of patients without macrovascular complica-tions for (15.31 ±3.13)μmol/L and (1.51 ±0.27)μmol/L(t =15.17,14.64,all P <0.05);Moreover,which were also higher than those in the healthy control group for (8.86 ±1.62)μmol/L and (0.85 ±0.14)μmol/L(t =26.29, 30.56,all P <0.05).The serum levels of Hcy and Cys C of type 2 diabetic patients with carotid arteries increased were (32.53 ±6.46)μmol/L and (2.83 ±0.52)μmol/L,which were higher than those of patients without carotid arteries for (18.46 ±3.63)μmol/L and (1.65 ±0.30)μmol/L(t =11.64,12.06,all P <0.05).In patients of type 2 diabetic with macrovascular complications,Hcy was positively correlated with serum Cys C(r =0.617,P <0.05). Conclusion sCD40L and YKL -40 may have important role in the pathogenesis of type 2 diabetic patients with ma-crovascular complications,which can be used as a valuable reference target in the diagnosis and monitoring of vascular complications in elderly type 2 diabetes,and it may be one of the important independent risk factors of patients with type 2 diabetes.

Gestational diabetes refers to the first occurrence or discovery of different degrees of abnormal glucose tolerance during pregnancy, which is one of the most common complications during pregnancy. As the incidence rate increased year by year, the complications of mother and child increased significantly. In recent years, gestational diabetes mellitus has been paid more and more attention. Most of the current researches are about the diagnosis and treatment of gestational diabetes 24 weeks and later, and the theoretical basis for the screening method and treatment of early gestational diabetes is still lacking. With the further understanding of early gestational diabetes mellitus and its adverse maternal and infant pregnancy outcomes, it is imperative to actively carry out the research on the screening and treatment of early gestational diabetes mellitus.

OBJECTIVETo perform a transcriptomics study in differential genes after Xianglian External Lotion (XEL) induced the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole.

METHODSBroth microdilution antifungal susceptibility test was used to detect minimal inhibitory concentration (MIC) of drug-resistant Candida albicans strains induced by XEL. Transcriptome sequencing (RNA-Seq) was used to determine and compare the transcription of primary drug-resistant Candida aIbicans strains and sensitive strains induced by XEL. High expressed genes and signaling pathways strains were analyzed by gene ontology (GO) method.

RESULTSXEL could induce drug-resistant strains of the 6th generations to recover sensitivity. Transcriptome sequencing showed that, as compared with primary drug-resistant strains, there were 165 genes with up-regulated RPKM index and 144 genes with down-regulated RPKM index after XEL induction. GO analyses found that all genes were mainly classified as GO:0015903 (fluconazole transport).

CONCLUSIONSXEL could induce the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole. By analyzing transcriptomes, authors speculated that XEL could recover strain sensitivity to fluconazole by opening fluconazole transport pathway.

Antifungal Agents ; pharmacology ; therapeutic use ; Candida ; Candida albicans ; drug effects ; genetics ; Candidiasis ; drug therapy ; Drug Resistance, Fungal ; drug effects ; genetics ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Fluconazole ; pharmacology ; Microbial Sensitivity Tests

Child ; Chromobacterium ; Female ; Humans ; Sepsis ; drug therapy ; etiology ; microbiology ; mortality