Adenocarcinoma ; pathology ; Gallbladder Neoplasms ; pathology ; Humans

Objective To investigate the role of nuclear factor ?B activation in the onset of acute coronary syndrome(ACS).Methods 76 patients with acute myocardial infraction(AMI),41 patients with unstable angina pectoris(UAP),43 patients with stable angina pectoris(SAP)and 20 normal controls were enrolled.NF-?B activation in monocytes in peripheral blood monocyte was determined by ELISA with the NF-?B p65 Kit the at 3 and 5 days after admission.Results The activity of NF-?B in monocytes of peripheral blood in AMI patients and UAP patients was significantly higher than that in SAP patients and normal controls(P

Objective To explore the anti-apoptosis mechanism of serum containing Xin Mai Tong decoction in myocardial cell under oxidative stress.Methods The neonate rat myocardial cell was cultivated and the model of myocardial cell apoptosis was induced with 400 ? mol/L H2O2.The cell viability was determined by MTT method,myocardial cell apoptosis was determined by TUNEL method,and the intra-cellular expression of HSP70,NF-? B and caspase-3 in myocardial cell was determined by immunocytochemistry.Results Administration of 400 ? mol/L H2O2 for 1 hour was harmless to myocardial cell,the expression of HSP70 being slightly increased.However,apoptosis was induced in myocardial cells at the 6 th hour after 400? mol/L H2O2 administration.The serum containing Xin Mai Tong significantly attenuated the decrease of cell viability and apoptosis which was induced by oxidative stress.Increased expression of HSP70 was induced by the serum containing Xin Mai Tong,and the activation of NF-? B and caspase-3 were significantly inhibited.Conclution The serum containing Xin Mai Tong decoction shows a significant inhibitory effect on apoptosis induced by H2O2,and the mechanism may be through increasing protein expression of HSP70,inhibiting the activation of NF-? B and decreasing the expression of caspase-3.

Objective To prepare the anti-hUART1 polyclonal antibody and investigate the subcellular localization of hURAT1 protein. Methods The full-length hURAT1 gene was obtained by RT-PCR and inserted into the fusion expression vector pEGFP-N3,the predicated antigen epitope was cloned into GST fusion protein expression plasmid pGEX-5X-1, transformed into E. coli BL21 cells for expressing recombinant GST-hURAT1 protein induced by IPTG. The purified hURAT1-GST fusion protein was employed to immunize rabbit for preparing the polyclonal antibody. The expression of hURAT1 was analyzed by western-blot and immunohistochemistry in human kidney. pEGFP-hURAT1 was transfected into the LLC-PK1 cell in order to observe the subcellular localization of the gene using confocal microscopy. Results Specific anti-hURAT1 rabbit polyclonal antibody was obtained, and both Western-blot and immunohistochemistry showed that hURAT1 was expressed in the human kidney brush border,localized in the apical membrane of the LLC-PK1 cell. Conclusions hURAT1 protein was a membrane protein located in renal proximal tubule, which could be detected in the apical membrane. The anti-hURAT1 polyclonal antibody could be used for studying the physiological function of hURAT1 and its pathology.

Humans ; Lymphoma ; classification ; pathology ; Lymphoma, B-Cell ; classification ; pathology ; Lymphoma, T-Cell ; classification ; pathology ; Lymphoproliferative Disorders ; pathology ; Pseudolymphoma ; pathology ; Skin Neoplasms ; classification ; pathology ; World Health Organization

Animals ; Blood Cell Count ; Blood Cells ; Decompression Sickness ; blood ; Male ; Rabbits

Cell Physiological Phenomena ; Electromagnetic Fields

OBJECTIVE: To assess the effect of disinfectant (Cavicide) with benzethon chloramine and isopropanol as main active ingredients disinfectant on dental impression accuracy. METHODS: The effect of Cavicide on three impression materials (alginate, polyether and vinylpolysiloxane) were assessed using a standard model. The standard model was digitized by an extraoral scanner (IScan D103i, Imetric). For each kind of impression materials, thirty impressions were taken following the manufactures' instruction in the same conditions. Subsequently, the impressions were randomly divided into three groups, with ten impressions in each group. After the impression taking was completed, the three groups underwent pure water rinse for 1 min (blank control, BC), 2% glutaraldehyde solution immersion disinfection for 30 min (glutaraldehyde, GD), and Cavicide solution spray disinfection for 5 min (Cavicide, CC), respectively. All the impressions were digitized by the extraoral scanner (IScan D103i, Imetric) after disinfection and exported to a dedicated three-dimensional analysis software (Geomagic Qualify 2014, Geomagic, USA). In the software, the digital models of the impressions were trimmed to teeth and then superimposed with the digitized standard model via best-fit alignment. Root mean square (RMS) was used to evaluate the deviations between the impression and the standard model. The deviation in the anterior and posterior regions was evaluated respectively. One-way ANOVA test and the LSD post-hoc test were used to compare the deviations between the three groups (P < 0.05). The color map of each superimposition was saved for visual analysis. RESULTS: For the polyether and vinylpolysiloxane materials, the difference between the three groups was not statistically significant (P=0.933, P=0.827). For the alginate material, the difference in posterior region between group GD and group BC, as well as group GD and group CC were statistically significant (GD vs. BC, P=0.001; GD vs. CC, P=0.002), while the difference between group BC and group CC was not statistically significant (P=0.854). The visual analysis showed an obvious deviation in the buccal-lingual direction in group GD. CONCLUSION Disinfectant (Cavicide) with benzethon chloramine and isopropanol as main active ingredients using spray disinfection has no effect on the accuracy of the alginate, polyether and vinylpolysiloxane impressions.
2-Propanol ; Chloramines ; Dental Impression Materials ; Dental Impression Technique ; Disinfectants ; Disinfection ; Models, Dental

Early hematoma enlargement in intracerebral hemorrhage seriously influences the prognosis of patients.This article reviews the incidence,diagnostic criteria,influence factors and prevention and treatment of early hematoma enlargement in intracerebral hemorrhage.

OBJECTIVE: Polymorphism of interleukin-23 receptor (IL-23R) gene was found being related with the susceptibility to several immune-related diseases. For the terminal differentiation of IL-17-producing effecter T-helper cells in vivo, the IL-23R gene is very important. As proved recently, Th17 cells might have a great influence to the pathogenesis of allergic airways disease. Our intention was paid to find any association between the polymorphisms in the IL-23R gene and allergic rhinitis(AR) in Chinese population. METHOD: A group of patients was involved in a case-control comparison, consisted of 239 AR patients and 271 control Chinese subjects. The study claimed to take some blood samples for DNA extraction and select 3 single-nucleotide polymorphisms in IL-23R individually genotyped by the PCR-RFLP method. RESULT: Comparison with the controls, a great growing prevalence of the homozygous rs7517847 GG genotype and G allele appeared in AR patients (P < 0.05, respectively). Besides, a great increased frequency of the GGA haplotypes was presented in AR patients in comparison with the controls (P < 0.05). CONCLUSION The results demonstrated a important association pattern between polymorphisms in IL-23R and AR in Chinese population. Between rs7517847 in an SNP of IL-23R and AR, a great association was identified.
Adolescent ; Adult ; Aged ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptors, Interleukin ; genetics ; Rhinitis, Allergic ; genetics ; Young Adult