Objective To provide data on the incidence,shape,and sizes of the femoral attach.ments of meniseofemoral ligaments (MFLs),and to determine the function of MFIJs and the role in the femoral tunnel D0sitioning in the double-bundle reconstruction of PCL.Methods Anatomical study:Thirty cadaveric knees were used to investigate the incidence and tightening-slackening patterns during the knee nexion-extension of MFLs.Data were obtained to describe the size,shape,and the relationship between the position of the femoral attachment of MFLs and that of anterolateral bundle(ALB)and the posteromedial bundle(PMB) were detetrained.Clinical study:During arthroscopic reconstruction of PCL,the presence of MFIJs Was confirmed in 1 12 patients by observation and probing,the relationship between the femoral attachment of MFLs and that of ALB and PMB were determined.Results Anatomical study:The Humphry ligament and Wrisberg ligament were presented in l(3%)and 27(90%)case respectively.The femoral attachments were approximately ellipse both.The mean area was(18.14±3.05)mm2 and(25.63±7.92)mm2 respectively.Clinical study:The Humphry ligament and Wrisberg ligament were present in 2 cases(1.7%)and 67 cases(59.8%) respectively.The incidence of MFLs in knees with isolated PCL injury was highter than that in knees with multiple ligaments injuries.Conclusion The incident of MFLs is related to the trauma of the knee.and the Wrisberg ligament plays an important role in avoiding impingement between the ALB and the intereondylar roof during deep knee flexion.The Humphry ligament and the Wrisberg ligament are landmarks for the tunnel positioning of ALB and PMB in double-bundle PCL reconstruction respectively.

Objective To study the clinical implementation strategy analysis about fast track surgery ( FTS) used in right co-lon cancer radical.Methods 72 patients with right colon cancer laparoscopic treatment in our hospital from June , 2013 to Decem-ber, 2014 were randomly selected and divided into observation group ( n=36 ) and control group ( n =36 ) by numeric randomized method.Observation group adopted treatments based on FTS while control group treated with conventional laparoscopic radical prosta-tectomy.Stress reaction, operation time, intraoperative blood loss, exhaust time, defecation time, stop the infusion time, drainage a-mount and postoperative drainage tube root out time, postoperative hospital stay and postoperative complications of two groups were sta-tistically analyzed.Results Preoperative blood cortisol, fasting blood glucose, serum IL-6 and plasma CRP level of two groups, op-eration time and compare differences of 2 groups had no statistical significance (p>0.05).Postoperative 24 h blood cortisol, fasting blood glucose, serum IL-6 and plasma CRP level of two groups were higher than that of preoperative.Postoperative 24 h blood corti-sol, fasting blood glucose, serum IL-6 and plasma CRP level of observation group were lower than that of the control group, and the difference was statistically significant (p<0.05).In observation group, the exhaust time, defecation time, drainage tube root out time, stop infusion time and postoperative hospital stay of observation group were (43.62 ±8.21) h, (50.42 ±6.85) h, (5.51 ± 1.08), (4.12 ±0.85)d and (5.42 ±1.13)d respectively, which were shorter than the (56.65 ±8.26)h , (64.78 ±8.26) h, (9.53 ±4.13)d, (7.78 ±1.43) d and (10.62 ±2.14)d of the control group, and the difference was statistically significant (p <0.05).Rainage amount of observation group[(602.54 ±165.78) ml] was less than that of the control group[(1265.28 ±572.94) ml];And the total complications incidence of observation group (8.33%) was lower than that of the control group (25.00%), and the difference was statistically significant (p<0.05).Conclusion Implementation of laparoscopic treatment based on FTS used in right colon cancer radical can effectively promote patients recovery and reduce complications.thus it is worthy of clinical promotion use.

Objective To determine the biomechanical properties of anterior cruciate ligament allograft reconstructing posterior cruciate ligament in rabbits. Methods Based on the study of anatomy and biomechanics of normal rabbit cruciate ligaments, anterior cruciate ligament allografts were employed to reconstruct the posterior cruciate ligament in rabbits. The sterilized fresh-frozen allograft of bone-anterior cruciate ligament-bone were prepared and reserved for more than 14 days under -80 ℃. Twenty-four skeletally matured New Zealand white rabbits underwent a posterior cruciate ligament reconstruction on one knee randomly, the opposite knee was served as the paired control. Rabbits were sacrificed at 6, 12, 26 and 52 weeks respectively. Evaluations of the reconstructions and contralateral controls included the geometric, structural and material properties and rupture site. Results The mean length of the grafts at 52 weeks was 101% of the control (P=0.90), the cross-sectional area was 142%; the maximum load at 52 weeks was 83% of the control, the maximum elongation was 72%, the stiffness was 92%; the maximum stress at 52 weeks was 58% of the control, the maximum strain was 72%, and the modulds was 65%; the rupture site was all at the body part of the graft. The geometric, structural and material properties of the graft were gradually similar to those of the normal posterior cruciate ligament with the elapse of the time. Conclusion The biomechanical properpies of graft with similar material properties to normal posterior cruciate ligament following posterior cruciate ligament reconstruction in rabbits was favourable. The similar material properties of graft to normal posterior cruciate ligament play the very important roles in the posterior cruciate ligament reconstruction.

Objective To explore the anti-apoptosis mechanism of serum containing Xin Mai Tong decoction in myocardial cell under oxidative stress.Methods The neonate rat myocardial cell was cultivated and the model of myocardial cell apoptosis was induced with 400 ? mol/L H2O2.The cell viability was determined by MTT method,myocardial cell apoptosis was determined by TUNEL method,and the intra-cellular expression of HSP70,NF-? B and caspase-3 in myocardial cell was determined by immunocytochemistry.Results Administration of 400 ? mol/L H2O2 for 1 hour was harmless to myocardial cell,the expression of HSP70 being slightly increased.However,apoptosis was induced in myocardial cells at the 6 th hour after 400? mol/L H2O2 administration.The serum containing Xin Mai Tong significantly attenuated the decrease of cell viability and apoptosis which was induced by oxidative stress.Increased expression of HSP70 was induced by the serum containing Xin Mai Tong,and the activation of NF-? B and caspase-3 were significantly inhibited.Conclution The serum containing Xin Mai Tong decoction shows a significant inhibitory effect on apoptosis induced by H2O2,and the mechanism may be through increasing protein expression of HSP70,inhibiting the activation of NF-? B and decreasing the expression of caspase-3.

Abstract: A new selaginellin derivative named as selaginellin S (1) was isolated from the whole plants of Selaginella pulvinata (Hook. et Grev.) Maxim. (Selaginellaceae), together with a known one (selaginellin M, 2). Compounds 1 and 2 were separated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were determined on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments, as well as ECD calculations. Compound 1 is a key intermidiant in the biosynthesis pathway of selaginellins. Compound 2 is first reported in this plant.
Biphenyl Compounds ; chemistry ; isolation & purification ; Cyclohexanones ; chemistry ; isolation & purification ; Molecular Structure ; Selaginellaceae ; chemistry

Objective To establish the animal model(rabbit) of shoulder instability for providing the experimental basis of related studies.Methods shoulder joints of 6 skeletally matured New Zealand white rabbits were measured with the anatomical characteristics and joint motion range in order to determine the operative approach,the similarities of skeletal structure,surrounding muscles,joint capsule and ligaments between rabbit and human were compared.Eighteen rabbits,weighing between 2.5 and 3kg were randomized into 2 groups(experimental group 12 and control 6).In experimental group,instability model was established by means of the operation procedure as follows:the glenohumeral joint of right shoulder was exposed by cutting the subscapularis tendon,then the anteroinferior labrum and surrounding capsular ligament were excised.For control group,sham operation was performed by only curing the skin and superficial tissue,the subscapularis tendon and glenoid structures was kept intact.The specimens of 6 rabbits were harvested 6 weeks after operation.The remaining rabbits of both experimental and control groups were killed 12 weeks post the operation for specimens processing.The motion range and instability were measured in all specimens.Results The gross anatomy of shoulder ioint in rabbit is similar to human.In experimental group,the stability is showed less than control,grade 2 anterior instability was found.The motion range was restricted in all directions,such as external and internal rotation,extension and abduction compared to control group.Conclusion Rabbit is suitable to establish animal model for shoulder instability and related iniuries,the animal model which was designed by this study could provide valuable information for the research of anterior shoulder instability.

This study examined the effect of astilbin on the proliferation of rat aortic smooth muscle cells (RASMCs) induced by angiotensin II (AngII) and explored the possible mechanisms. Cell proliferation model of RASMCs was induced by treatmente with AngII. Cells were randomly divided to 8 groups. Normally cultured VSMCs serves as blank control group; in AngII model group, cells were treated with AngII at 10(-7) mol/L; in three astilbin groups, cells were treated with 10, 15, 30 mg/L of astilbin; in three AngII+astilbin groups, cells were treated with AngII (at 10(-7) mol/L) and astilbin at 10, 15, 30 mg/L. Cell proliferation ability was detected by MTT method and the cell cycles and proliferation index were flow cytometrically determined. The expression of c-myc mRNA was assessed by using reverse transcription polymerase chain reaction (RT-PCR), and the expression of NF-κB in RASMCs was immunocytochemically observed. Our results showed that MTT metabolism in RASMCs in the basic and AngII stimulated situation was inhibited by astilbin, and the cells numbers of G(0)/G(1) phase were increased and that of G(2)/S phase were decreased markedly. Not only highly expression of c-myc gene stimulated by AngII could be inhibited by Astilbin significantly, but also the expression of NF-κB protein can be down regulated by Astilbin. We are led to conclude that astilbin astilbin can inhibit the AngII-mediated proliferation of RASMCs by blocking the transition of RASMCs from G(0)/G(1) phase to S phase and by down-regulating the expression of NF-κB, c-myc gene.

Two isomers of nitrochlorobenzene (o-, and p-NCB) were treated by a Pd/Fe catalyst in aqueous solutions through catalytic amination and dechlorination. Nitrochlorobenzenes are rapidly converted to form chloroanilines (CAN) first through an amination process, and then rapidly dechlorinated to become aniline (AN) and Cl(-), without the involvement of any other intermediate reaction products. The amination and dechlorination reaction are believed to take place predominantly on the surface site of the Pd/Fe catalysts. The dechlorination rate of the reductive degradation of the two isomers of nitrochlorobenzene (o-, and p-NCB) in the presence of Pd/Fe as a catalyst was measured experimentally. In all cases, the reaction rate constants were found to increase with the decrease in the Gibbs free energy (correlation with the activation energy) of NCBs formation; the activation energy of each dechlorination reaction was measured to be 95.83 and 77.05 kJ/mol, respectively for o- and p-NCB. The results demonstrated that p-NCBs were reduced more easily than o-NCBs.
Catalysis ; Industrial Waste ; prevention & control ; Iron ; chemistry ; Isomerism ; Kinetics ; Metals ; chemistry ; Nitrobenzenes ; chemistry ; Palladium ; chemistry ; Structure-Activity Relationship ; Waste Disposal, Fluid ; methods ; Water ; chemistry ; Water Purification ; methods

OBJECTIVESTo provide the data on the shape, sizes, and locations of the attachments of the anterolateral bundle (ALB) and posteromedial bundle (PMB) of the posterior cruciate ligament (PCL) to the tibia, and to determine the reference landmarks and the methods for tibial tunnel positioning in double-bundle PCL reconstruction using double-double tunnel.

METHODSThirty cadaveric knees were used as specimens. PCLs were separated into bundles according to the PCL tension pattern during knee flexion-extension, and the functional bundle in each bundle was determined. Data were obtained to describe the size, shape, position, and center of the attachments of PCL bundles. The reference landmarks and methods for tibial tunnels positioning in double-bundle PCL reconstruction using double-double tunnel were determined.

RESULTSThe PCL insertion site was situated in the posterior intercondylar fossa. The longitudinal axis of the tibial attachment of PCL proceeded from proximal medial to distal lateral, and the mean angle between them and the tibial shaft was (16.5 +/- 1.4) degrees . The tibial insertion site of ALB and PMB were arranged in the proximal and distal on the whole, the tibial attachment of ALB could generally be described as rhomb in shape, and the mean area of it was (90 +/- 20) mm(2); the tibial attachment of PMB was rectangle in shape, the mean area of it was (96 +/- 32) mm(2), there was no statistic difference between the area of them (P > 0.05). There were functional bundles in both ALB and PMB persistently, which attached to the distal-lateral portion of the tibial attachment of ALB and the distal-medial portion of the tibial attachment of PMB respectively, they were both oval in shape, the mean area of them were (35 +/- 12) mm(2) and (36 +/- 6) mm(2) respectively, the difference between them was no statistic significant (P > 0.05). The mean distance between the centres of the tibial attachments of the functional bundles of ALB and that of PMB was (12.7 +/- 1.9) mm. The medial tibial spine, lateral tibial spine and the bony ridge on the posterior-proximal tibia were the key anatomic landmarks that could be used to aid in placement of independent tibial tunnels for a 2-bundle PCL reconstruction.

CONCLUSIONSThe tibial attachment of the PCL is sufficiently large to allow for placement of 2 independent tunnels. There are functional bundles in both ALB and PMB persistently and the optimum position for the tibial tunnel in double-bundle PCL reconstruction using double-double tunnel should be located in the attachment sites of the functional bundles of ALB and PMB.

Aged ; Female ; Humans ; Male ; Middle Aged ; Posterior Cruciate Ligament ; anatomy & histology ; surgery ; Tibia ; anatomy & histology ; surgery

Mechanical properties and biological evaluation of buffalo horn material were examined in this study. The effects of sampling position of buffalo horn on mechanical properties were investigated with uniaxial tension and micron indentation tests. Meanwhile, the variation of element contents in different parts of buffalo horn was determined with elemental analysis, and the microstructure of the horn was measured with scanning electron microscopy. In addition, biological evaluation of buffalo horn was studied with hemolytic test, erythrocyte morphology, platelet and erythrocyte count, and implantation into mouse. Results showed that the buffalo horn had good mechanical properties and mechanical characteristic values of it gradually increased along with the growth direction of the horn, which may be closely related to its microstructure and element content of C, N, and S in different parts of the buffalo horn. On the other hand, because the buffalo horn does not have toxicity, it therefore does not cause hemolysis of erythrocyte and has a good affinity with it. Buffalo horn has good histocompatibility but meanwhile it may induce the platelet adhesion and aggregation. Even so, it does not continue to rise to induce a large number of platelet to aggregate with resulting blood clotting. Therefore, the buffalo horn material has been proved to possess good blood compatibility according to the preliminary evaluation.
Animals ; Biocompatible Materials ; Biomechanical Phenomena ; Buffaloes ; Erythrocytes ; Horns ; chemistry ; ultrastructure ; Mice ; Microscopy, Electron, Scanning