Objective:To study the relation between the xenograft survival and IgG, M?, NK,CD4, CD8,MLR testing in peripheral blood of the recipient Methods:By using mouse to rat heart tissue transplantation model,4 experimental groups were established and treated by using: CCV, RPMI 1640(Ⅰ);splenocyte, anti serum with CCV therapy (Ⅱ); splenocyte, anti serum with CsA, Cy, CCV therapies (Ⅲ);splenocyte, anti serum with CsA, Cy, CCV, anti M?, anti NK, anti CD4 and anti CD8 therapies (Ⅳ) The rats received splenocytes (1?10 8) intravenously on the day -12, -8, -4, 0;anti serum (0 2 ml) on the day -10, -6, -2, 0, and CsA 〔10 mg/(kg?d) i p〕, Cy 〔20 mg/(kg?d) i p〕, CCV 〔0 2 mg/(kg?d) i p〕, anti CD4,anti CD8,anti M? and anti NK 〔250 ?g/(kg?d)〕 on the day from 0 to 6 relative to heart grafting on day 0 The graft survival, the IgG, CD4,CD8, M?, NK, MLR were studied Results: On the 7th day after transplantation, grafts in groupⅠwere destroyed, but the grafts in group Ⅱ, Ⅲ, Ⅳ survived well; on the 21st day, only grafts in group Ⅳsurvived well (P

Objective To establish the computer controlled operant behavior test system for rats,and to explore the effects of prenatal exposure to low level methylmercury on the ability of learning and memory in rats Methods The Wistar rats were perorally perfused by 0,0.01,0.05,2.00 mg/(kg?d) methylmercury chloride(MMC) during the period from the 6th day to the 9th day after conception respectively.In every dose group,8 offspring aged 10 weeks were randomly selected to be tested by operant behavior test system.The operant performance was measured in automated nocturnal 15 hour session(16∶00~7∶00).The trial pragram Differential Reinforcement of High (DRH) rate and of low (DRL) rate was carried out by a double blind method.The DRH tests required observed animals to press levers frequently,while the DRL test required the subjects to respect a blocking interval after a reinforcement for pressing lever again. Results The performances scores of 3 exposure groups at doses of 0.01,0.05 and 2.00 mg/(kg?d) methylmercury were significantly lower than that of the control group( P

Objective:To explore the possibility of inducing cell mediated immune response with HSP70 antigenic peptide complex in vitro.Methods:HSP70 peptide complex was reconstituted in vitro.Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of HLA A2 positive healthy donors.HSP70,HSP70 peptide complex or peptide was used to activate the DC individually,which will initiate homogenize T lymphocyte to form cytotoxic T lymphocyte(CTL).The cytotxicity of the CTL was detected by MTT assay.Results:It was found that peptide specific CD8 + CTL responses were readily elicited by HSP70 peptide complex or peptide.The CTL response primed by HSP70 peptide complex was more potene than peptide alone.Conclusion:The results suggest that HSP70 peptide complex is immunogenic and HSP70 can lead to great efficient CTL response,antigenic peptides and HSP70 complex may be used as peptide vaccines for cancer immunotherapy.

Objective:In view of the immunological and clinical manifestation of SIgAD.Methods:Single radial immunodiffusion technique was emploied to determine serum IgG?IgA?IgM and the enzyme linked immunosorbent assay(ELISA) had been used to measure serum IgE;anti unclear antibodies(ANA) were test with indirect immunofluorescence technique,cellular immunity was test with lymphocyte translation assay.Results:Tweenty eight patients with selective IgA deficiency were seen during the last tween years(IgA

Purpose:To evaluate the therapeutic effect of paclitaxel and platinum containing chemotherapy (cisplatin or carboplatin) in women with epithelial ovarian cancer.Methods:We assigned 16 cases with advanced epithelial ovarian cancer after initial surgery to receive paclitaxel and cisplatin or carboplatin. Pcalitaxel (135 mg/m 2,over a period of 10 hours ) was administered iv once every 3 to 4 weeks, 24 control cases were treated with "CAP" or "CVP" regimen. Results:The overall median survival was significantly longer in the paclitaxel platinum group than in the cisplatin cyclophosphamide group (median, 35 vs. 22.5 months, P

Objective:To investigate the expression of membrane cofactor protein (MCP)?decay accelerating factor (DAF) and homologous restriction factor 20 (HRF 20) on the surface of CD16 +monocyte/macrophage (Mo/M?) in the urine of patients with glomerulonephritis(GN) Methods:The expression of MCP?DAF and HRF 20 on the surface of urinary CD16 +Mo/M? were tested by Flow Cytometry and the levels of urinary sC5b 9 by ELISA in 134 patients with GN that were divided into mini change (MC)?glomerulosclerosis (GS)?membranous nephropathy (MN) and proliferative glomerulonephritis (PGN) Results:The expressions of MCP?DAF and HRF 20 on the surface of urinary CD16 +Mo/M? and the levels of urinary sC5b 9 in the patients with GS ?MN or PGN were significantly higher than those in controls (P

Objective:To explore the possibly mechanisms of inducing allogeneic chimerism and prolonging allografts survival in recipients by immature dendritic cells (imDCs) Methods:Bone marrow cells (BMCs) derived from donor (C57BL/6) were used to generate imDCs The splenocytes of recipients were pretreated by inactivated imDCs in vitro, or the recipients (Balb/C) were injected of inactivated imDCs via vein in vivo Then collected the splenocytes mixed with the inactivated splenocytes from donor to detect the responsiveness Mixed lymphocyte reaction was also used to evaluate the reactivity of the chimerism mice to the donor splenocytes At the same time the diversion of Th1/Th2 paradigm was studied by semi quantitative RT PCR Results:The splenocytes conditioned with imDCs pretreatment expressed hypo responsiveness to the donor stimulation, and the immunized mice also proliferated less degree compared with the naive mice The hyporeactivity was evidently seen within 72 hours after stimulation by donor splenocytes There was significant difference between them The chimerism mice showed unresponsiveness to donor antigens, while reactivity to the third party antigens was retained The result of RT PCR suggested, to some extent, there was a diversion of Th1/Th2 paradigm in the establishment of chimerism in the model Conclusion:The putative mechanism of immature dendritic cells inducing the generation of allogeneic chimerism may based on the hypo responsiveness produced by imDCs, and there may also exist some kinds of diversion of Th1/Th2 paradigm

Objective To investigate the effect of a self made liposome perfluocarbon ultrasound contrast agent in enhancing grey scale imaging of normal kidney.Methods The kidneys of 10 normal rabbits were imaged with second harmonic imaging before and after intravenous injection of the liposome contrast agent at a dose of 0.01 ml/kg. The subjective scores and video intensities were used to evaluate contrast effects. Results The renal cortex,medulla and sinus grey scale imaging were greatly enhanced after injection, and the notable enhancement time of renal cortex,medulla and sinus could last for 52 min,2 min 20 s,1 min 40 s,respectively. Conclusions The self made liposome perfluocarbon contrast agent can significantly long time enhance the grey scale of the kidney.

Objective To measure the width of space in front of the femoral neck at different age of normal children for diagnosis such as hip disease in children as bilateral transient synovitis of the hip. Methods Four hundred and twenty normal children below 14 years old were divided into 14 groups according to their age. Every group contained 30 children. They underwent bilateral ultrasonographic assessment. The width of space in front of the femoral neck was measured and collected. The mean width value in each group was compared mutually by statistical analysis (t test). Results The mean width of space in front of the femoral neck of normal children below 14 years old was 2.0 - 5.9 mm, the range was 1.2 - 7.8 mm. Conclusions The width of space in front of the femoral neck is different at different age of normal children, the mean value below 14 years old

Objective:To investigate the effect of glucocorticoid on treating class IV lupus nephritis(LN) by comparing the content of serum transforming growth factor beta(TGF ? 1).Methods:The study consisted of 33 objects,15 health controls as well as 18 cases of IV LN.The level of serum TGF ? 1 was analyzed by ELISA.Results:The level of serum TGF ? 1 was greatly reduced after the patients were treated with glucorcorticoid.Conclusion:Timely and sufficient glucocorticoid may prevent IV LN from developing into glomerulosclerosis.