This study was designed to identify the pathogen causing soft rot of Pinellia ternata in Qianjiang of Hubei province and screen out the effective bactericides, so as to provide a theoretical basis for the control of soft rot of P. ternata. In this study, the pathogen was identified based on molecular biology and physiological biochemistry, followed by the detection of pathogenicity and pathogenicity spectrum via plant tissue inoculation in vitro and the indoor toxicity determination using the inhibition zone method to screen out bactericide with good antibacterial effects. The control effect of the bactericide against P. ternata soft rot was verified by the leave and tuber inoculation in vitro. The phylogenetic tree was constructed based on the 16 S rDNA, dnaX gene, and recA gene sequences, respectively, and the result showed that the pathogen belonged to the same branch as the type strain Dickeya fangzhongdai JS5. The physiological and biochemical tests showed that the pathogen was identical to D. fangzhongdai, which proved that the pathogen was D. fangzhongdai. The pathogenicity test indicated that the pathogen could obviously infect leaves at 24 h and tubers in 3 d. As revealed by the indoor toxicity test, 0.3% tetramycin, 5% allicin, and 80% ethylicin had good antibacterial activities, with EC_(50) values all less than 50 mg·L~(-1). Tests in tissues in vitro showed that 5% allicin exhibited the best control effect, followed by 0.3% tetramycin and 10% zhongshengmycin oligosaccharide, and their preventive effects were better than curative effects. Therefore, 5% allicin can be used as the preferred agent for the control of P. ternata soft rot, and 0.3% tetramycin and 10% zhongshengmycin oligosaccharide as the alternatives. This study has provided a certain theoretical basis for the control of P. ternata soft rot.
Phylogeny ; Pinellia/chemistry* ; Plant Leaves ; Plant Tubers

The study aims at investigating the grade standard and the quality characteristic of Pinelliae Rhizoma for commodity specification, which provides the reference for its grade standard formulation. 42 Pinelliae Rhizoma simples were collected from 5 medicinal materials markets and 2 producing areas. Based on the previous herbalogical study and market investigation, we combined with the data analysis to select the grading indicators using SPSS software for descriptive statistical analysis, analysis of variance, K-cluster analysis and correlation analysis. According to the actual production condition, we developed the grading standards of Pinelliae Rhizoma. Moreover, we compared the internal indicators(water, total ash, leachate and guanosine) of Pinelliae Rhizoma at various grade levels, and analyzed the correlation between appearance traits and internal indicators. The herbalogical study and market research found that the Pinelliae Rhizoma was better in large, solid and white. The results from descriptive and variance analysis showed that the appearance of Pinelliae Rhizoma was significantly different in weight per grain and grain number of 500 g. Referring to the 2015 Chinese Pharmacopoeia and the production practice, we use the length, weight per grain and grain number of 500 g as the classification index of Pinelliae Rhizoma. The results from correlation analysis showed that there was no significant correlation between the appearance of Pinelliae Rhizoma and the intrinsic quality index. In addition, we found there was no significant difference in the content of the intrinsic index except for the total ash and the extract. The current study established the classification index of the product specification and grade standard of Pinelliae Rhizoma with length, weight per grain and grain number of 500 g as the index, which can provide the basis for the classification of the product specification and grade of Pinelliae Rhizoma market.
Drugs, Chinese Herbal ; standards ; Pinellia ; chemistry ; Rhizome ; chemistry

OBJECTIVETo explore the current application and features of Aconite prescriptions with incompatible herbs in grade A class three hospitals in east China and central China through a clinical study and comparative analysis.

METHODClinical prescriptions containing Aconite with incompatible herbs were collected. Association rules were utilized to analyze the compatible features of these herbs.

RESULTThis analysis found that the frequently used incompatible herba; pairs are Aconiti Lateralis Radix Praeparata-Pinelliae Rhizoma, with the support rate of 44.45%, occupying nearly half of the surveyed prescriptions; Pinelliae Rhizoma is the most frequently used herb in the two areas, with support rate up to 76.24%. Among the top 10 herbal pairs in the support rate, except for Aconiti Lateralis Radix Praeparata and Pinelliae Rhizoma, the top 10 herbs in Central China were mostly for warming the middle jiao and tonifying qi, such as Zingiberis Rhizoma, Atractylodis Macrocephalae Rhizoma and Codonopsis Radix; Whereas those in east China were mostly for activating and nourishing blood, such as Angelicae Sinensis Radix, Chuanxiong Rhizoma, and Salviae Miltiorrhizae Radix et Rhizoma. Among the top 10 herbal pairs in the support rate, except for Aconiti Lateralis Radix Praeparata-Pinelliae Rhizoma, the core herbal pairs applied in central China were mainly for resolving phlegm and warming the middle jiao, such as Pinelliae Rhizoma-Glycyrrhizae Radix et Rhizoma, Pinelliae Rhizoma-Zingiberis Rhizoma; Whereas those in east China were principally for activating blood and tonifying qi, like Atractylodis Macrocephalae Rhizoma and Pinelliae Rhizoma, Angelicae Sinensis Radix and Pinelliae Rhizoma. Among the core herbal groups in the two areas, the most frequently used herbal groups in the two areas are Aconiti Lateralis Radix Praeparata, Glycyrrhizae Radix et Rhizoma and Pinelliae Rhizoma with the support rate of 59.73%, accounting for the highest proportion among all of herbal groups.

CONCLUSIONThere are the combined clinical application of Aconite with incompatible herbs, mostly with Aconiti Lateralis Radix Praeparata-Pinelliae Rhizoma, but with differences in the combined application in east China and central China.

Aconitum ; chemistry ; Drug Incompatibility ; Drug Prescriptions ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Pinellia ; chemistry ; Sophora ; chemistry

OBJECTIVETo explore the influence on AM fungi infection rate and medicine quality of Pinellia ternate in the condition of three soil impact factors.

METHODSet the orthogonal test of three factors and levels. Determinate the AM fungi infection rate in early stage of mature & stage of mature of P. ternata, and the water content, water soluble extract, butanedioic acid content and alkaloid content of P. ternata tuber that be harvested also had be determinated.

RESULT AND CONCLUSIONWith the P levels to 30 mg x kg(-1) and 90 mg x kg(-1), AM fungi infection was the best when mixed inoculated of EM. Microbial agent inoculated played a decisive role in P. ternata growth and physiological activity, secondary influenced factor was P concentration, and the water stress was the minimal impact. Mixed inoculated of AM fungi and EM treatment with the low P levels (30, 90 mg x kg(-1)) proved better effect on enhancing the water extract content, anedioic acid and alkaloid content, while decreasing the water contents of P. ternata tuber.

Alkaloids ; chemistry ; Drugs, Chinese Herbal ; chemistry ; standards ; Fungi ; Pinellia ; chemistry ; microbiology ; Plant Extracts ; chemistry ; standards ; Soil

OBJECTIVETo study the change of photosynthesis and chlorophyll fluorescence characteristics of Pinellia ternata around sprout tumble.

METHODTubers of P. ternata were cultured firstly at (25 +/- 1) degrees C for certain days, and then they were coerced under 37 degrees C stress in the same artificial climate boxes. The chlorophyll contents, photosynthesis and chlorophyll fluorescence during different stages of high temperature stress were measured.

RESULT AND CONCLUSIONAfter under high temperature stress, the chlorophyll contents, the ratio of chlorophyll a/b, photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximum fluorescence (Fm), quantum yield of PS II (phi (PS II)), intrinsic photochemical efficiency of PS II (Fv/Fm) and the chlorophyll photochemical quenching (qP) decreased, but the intercellular CO2 concentration (Ci), minimal fluorescence (F0), the chlorophyll nonphotochemical quenching (NPQ) increased.

Chlorophyll ; chemistry ; metabolism ; Fluorescence ; Hot Temperature ; Photosynthesis ; Pinellia ; chemistry ; physiology ; Plant Extracts ; chemistry ; metabolism ; Stress, Physiological

OBJECTIVETo establish the seed quality grading standard of artificial tuber of Pinellia ternata.

METHODHundred-grain weight, moisture content, germination rate, viability, single-grain diameter, purity and visual form of mature artificial tuber seed from tissue culture of P. ternate were determined. The key indicator and reference indicators for artificial tubers quality grading were defined statistically by SPSS analysis.

RESULTGermination rate and viability were the primary indicators, hundred-grain weight, moisture content and purity were important reference indicators.

CONCLUSIONThe quality of each grade should reach the following requirements: for first grade, hundred-grain weight > 10 g, moisture content > 70%-80% , germination rate > 80%, viability > 85%, single-grain diameter > 0.7 cm, purity > 80%; for second grade, hundred-grain weight 8-10 g, moisture content > 70%-80%, germination rate 60%-80%, viability 70%-85%, single-grain diameter 0.3-0.7 cm, purity 75%-80%; moisture content > 70%-80%, and the others don't reach second grade was the third grade.

Germination ; Pinellia ; chemistry ; physiology ; Plant Tubers ; chemistry ; physiology ; Quality Control ; Seeds ; chemistry ; physiology

In the clinical practice of traditional Chinese medicine, Pinelliae Rhizoma is a commonly used traditional Chinese medicinal material, with a very important medicinal value, but its raw products have certain toxicity. According to the basic theory of traditional Chinese medicine, after Pinelliae Rhizoma is processed, its toxic and side effects can be reduced, there by ensuring its medication safety in clinic. Based on the processing characteristics of Pinelliae Rhizoma replication method, this article discusses the changes before and after processing of Pinelliae Rhizoma with multiple materials and multiple processes. This method does not have a unified process. One or more materials are added, and various processes, such as dipping, soaking, bleaching, or steaming, cooking or combined methods are adopted. Then, Pinelliae Rhizoma is repeatedly processed to meet the specified quality requirements. By different processing methods, the efficacy changes accordingly, and Pinelliae Rhizoma can be used for the treatment of different diseases. The article starts in the two directions of multi-materials and multi-processes, and summarizes the effects of multi-materials or multi-processes on the chemical composition and efficacy, as well as the processing mechanism of reducing toxicity and increasing efficiency, in the expectation of reducing the toxicity and enhancing the efficacy of Pinelliae Rhizoma. The medicinal scope provides a reference and theoretical basis for further studies on the processing mechanism. An in-depth study is conducted to make Pinelliae Rhizoma more safe and effective in clinic application, and ensure its clinical efficacy. It will provide reference for future studies on quality control, active ingredients and new drug development of the processing of pinellia slices. It has laid a scientific foundation for exploring the best processing techno-logy, and provided a theoretical basis for solving the production problems of processed Pinelliae Rhizoma products, so as to improve the production efficiency and the quality of medicines.
Drugs, Chinese Herbal/chemistry* ; Medicine, Chinese Traditional ; Pinellia/chemistry* ; Rhizome/chemistry* ; Technology, Pharmaceutical

OBJECTIVETo study the processing technics of prepared slice of Rhizoma Dioscoreae for its industrial production.

METHODThe effect factors, such as stiring temperature (60, 80, 100 degrees C), stiring time (10, 20, 30 min) and the proportion of pieces and wheat bran (100: 5, 100: 10, 100: 15) were evaluated by orthogonal method. The content of allantoin in Rhizoma Dioscoreae was determine by HPLC method.

RESULTThe amount of wheat bran could affect the content of allation significantly, and stiring temperature and stiring time almost had no effect on content of allantoin. The processing technics was tested by industrial produce, according to 11 batches from 4 growing regions. The result showed that the quality of industrial product was stable.

CONCLUSIONThat the amount of wheat bran can affect the quality of Rhizoma Dioscoreae greatly. The determined technics is simple and suitable for prepared slice of Rhizoma Dioscoreae industrival manufacture.

Allantoin ; analysis ; Dietary Fiber ; analysis ; Drug Compounding ; methods ; Drugs, Chinese Herbal ; chemistry ; Pinellia ; chemistry ; Technology, Pharmaceutical

OBJECTIVETo study the photosynthetic characteristics and chlorophyll fluorescence parameters of Pinellia ternate from different sources, in order to provide basis for cultivation and breeding.

METHODPigment contents were compared among different sources by the Lichtenthaler method. The light-response and diurnal photosynthesis course of P. ternata from different sources were analyzed by LI-6400 system, and chlorophll fluorescence parameters were analyzed by PAM-2100 system.

RESULTThe difference of photosynthetic characteristics among the sources were remarkable. The light compensation point (LCP) of P. ternate from different sources were 11.57-17.88 micromol x m(-2) x s(-1), the light saturation point (LSP) were 1 000-1 300 micromol x m(-2) x (s-1). All the sources have obvious "midday depression". P. ternate cultivated in Xinjiang showed a higher light saturation point (LSP) and better chlorophyll fluorescence parameters.

CONCLUSIONP. ternata cultivated in Xinjiang showed better photosynthetic characteristics and chlorophyll fluorescence parameters than the others.

China ; Chlorophyll ; chemistry ; Fluorescence ; Light ; Photosynthesis ; Pinellia ; chemistry ; physiology ; radiation effects

OBJECTIVETo study the dynamic accumulation of dry matter and index components and provide reference to the manual cultural technique of Pinellia ternata.

METHODSeedlings of Taizhou P. ternata were used as the pot experiment material, and the contents of free total organic acid and guanosine of each organ were determined at different stages of spring and autumn period.

RESULTP. ternata had two growth climax in spring and autumn. Under the same condition of cultivation and management, dry matter largely accumulated in spring. The contents of free total organic acid and guanosine was ascending during the growth period, but dropped during sprout tumble bolting and sprout tumble stage.

CONCLUSIONHigh temperature and bolting affect the accumulation of organic acids significantly, it is reasonable to prevent the temperature stress in production.

Acids ; metabolism ; Guanosine ; metabolism ; Hot Temperature ; Pinellia ; chemistry ; metabolism ; Seasons ; Seedlings ; chemistry ; metabolism