The optimal treatment of elderly women with breast cancer still faces many difficulties. Life expectancy, comorbidity, and functional status influence clinical decision and survival. Standard adjuvant medical treatments should not be withheld from healthy older patients with reasonable life expectancies. In this study, adjuvant medical treatment literatures for elderly breast cancer patients, including endocrine therapy, chemotherapy, and HER2-targeted therapy, were reviewed, focusing on efficacy and toxicity of drugs for elderly women.

Objective: To study the stability of forsythiaside. Methods: The stability of forsythiaside was studied by using UV spectrum obsiving the changes of spectrums in different conditions. Results: Forsythiaside was very unstable under the condition of alkali (pH=9.40), relatively stable at the pH=6.86, 60?Cand stable at the pH= 4.03 . Conclusion: The stability of forsythiaside can be affected by the condition of the acid, alkali and temperatures obviously.

Patients with HER-2-positive advanced breast cancer were associated with poor prognosis. Meanwhile, HER-2-targeted ther-apy has dramatically improved survival and prognosis among breast cancer patients. Over the years, multiple HER-2-targeting drugs stepped into clinical practice, and the targeted agents are now considered as the standard of care in the first-line setting and beyond. This review basically summarizes the importance of HER-2-targeted therapy, the significance of the clinical trial results, and the clinical practice guidelines for the management of patients with HER-2-positive advanced breast cancer.

Adult ; Aged ; Aged, 80 and over ; Antineoplastic Agents ; adverse effects ; therapeutic use ; Brain Neoplasms ; secondary ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; secondary ; Exanthema ; chemically induced ; Female ; Humans ; Liver Neoplasms ; secondary ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Protein Kinase Inhibitors ; adverse effects ; therapeutic use ; Quinazolines ; adverse effects ; therapeutic use ; Remission Induction ; Survival Rate ; Vomiting ; chemically induced

Objective: To investigate the antidepressant effect and mechanism of the extract of mulberry leaf(EML). Methods: Male BALB/c mice were randomly assigned to receive EML(100, 200 and 400 mg/kg)or vehicle for 7 days or single administration by intragastric(ig)route. The effect of EML on spontaneous activity in mice was evaluated using the open field test. The anti-depressant activity of EML was evaluated using the tail suspension test and forced swimming test. After single ig administration of EML (100, 200 and 400 mg/kg), the effects of EML on the function central monoaminergic nervous system were evaluated using the 5-hydroxy-L-tryptophan(5-HTP)induced head-twitch test, yohimbine toxicity potentiation test and reserpine test in mice. Results: In the behavioral despair model, the continuous administration of EML(100 mg/kg)for 7 days significantly reduced the immobility time in the tail suspension test and forced swimming test. Compared with the vehicle group, the inhibitory rates of immobility time were 35.5% and 41%(P<0.05, P<0.01)in the tail suspension test and forced swimming test, respectively. The single treatment with EML(400 mg/kg)also significantly reduced the immobility time in the tail suspension and forced swimming test, and the inhibitory rates of immobility time were 29.1% and 35.3%, respectively(P<0.05). The results of spontaneous activity test showed that EML had no excitatory or inhibitory effect on the central nervous system in mice. In the 5-HTP induced head-twitch test in mice, the single treatment with EML(100 mg/kg)significantly increased the number of head-twitches in mice. In the yohimbine toxicity potentiation test, the single treatment with EML(100, 200 and 400 mg/kg)had no significant effect on the mortality rate in yohimbin-treated mice. In the reserpine test, compared with the model group, the single treatment with EML(400 mg/kg)antagonized reserpine induced ptosis (P<0.05)and had no significant effect on the decrease in rectal temperature and akinesia. Conclusion: EML showed antidepressant effect, and the action mechanism was likely related to the enhancement of the function of serotonergic nervous system.

The review is focused on the development and recent research of the first tissue-specific angiogenic molecule,endocrine gland-derived vascular endothelial growth factor(EG-VEGF).It has been found that EG-VEGF only targeted on steroidgenic endocrine organs or cells through G-protein coupled receptor,the phosphorylation of G-protein coupled receptor could activate mitogen-activated protein kinase p44/42 and phosphatidylinositol-3 kinase(PI3K) pathways signal transdution.There are many reports that believes MAPK and PI3K is involved in the regulation of proliferation,migration,survival of endothelial cells and tumor angiogenesis,so EG-VEGF may serve as a potential target for antiangiogenic therapy of cancer in the future.

OBJECTIVE:To improve automatic outpatient pharmacy drug delivery system in our hospital,and to promote the efficiency of automatic drug delivery. METHODS:The outpatient pharmacy automation system of our hospital was introduced in terms of main structure,working process and application. The work efficiency,safety and error were compared between automatic drug delivery mode and manual drug delivery mode. Drug delivery machine was improved to increase the rate of drug delivery. RE-SULTS:The outpatient pharmacy automation system of our hospital included 2 boxed drug delivery machine and 1 drug dispensing machine,etc. Compared with manual drug delivery mode,automatic drug delivery mode improved drug delivery efficiency,health security and use security,and reduced drug delivery error. Through adjusting the time setting and step of drug delivery of gearing in drug delivery machine,idle time of transmission equipment was utilized effectively,and the quantity of delivered prescriptions was increased,increasing from 220 to 320 each hour. CONCLUSIONS:The reasonable improvement of outpatient pharmacy auto-mation system makes outpatient pharmacy automation system play a bigger role,and further optimize resources.

AIM: Human herpesvirus 6B(HHV-6B) is the etiologic agent of exanthem subitum and is associated with febrile illnesses in children, neurological manifestations during primary infection, and clinical complications in organ transplant patients. The ORF U83 of HHV-6B has been identidied encoding a functional chemokine and U83 protein was detected out in brain tissues of some patients with multiple cirrhosis. The aim of this study was to establish HHV-6B U83 transgenic mouse lines, which would provide a pathological animal model for the research of the relationship between HHV-6B U83 gene and multiple cirrhosis. METHODS: Transgenic animal technique was applied in the present study. RESULTS: We successfully established 3 lines of transgenic mice with HHV-6B U83 gene integrated to their chromosomes, through constructing the DNA fragment which consisted of GFAP promotor, HHV-6B U83 and mouse protamine 1 sequence and injecting to the mouse eggs. CONCLUSION: This establishment of HHV-6B U83 transgenic mouse lines would benefit the observation and analysis of association of HHV-6B infection with neurological system diseases.

Objective To select polyethylenimine polymer with proper molecular weight to enhance the gene expression efficiency of Adenovirus (Ad).Methods Ad was resepcitively complexed with PEI-600,PEI-1 800 and PEI 25 000 by electrostatic adsorption.Afterwards,MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay was used to investigate the cytotoxicity of the complexes with a serial concentration,followed by cell transduction assay to select the best amount of PEI with fixed dose of Ad.In the further,the physicochemical characteristics of the optimized complexes,Zeta potential,and size distribution were studied.Results MTT showed the higher the PEI moderate molecular weight was,the greater the cytotoxity was.PEI-25 000 showed a greater cytotoxicity than that of PEI-600 and PEI-1 800.As compared with PEI-600 and PEI-25 000,PEI-1 800 significanlty promoted expression of Ad gene in A549 cells.PEI turned the Zeta potential of Ad from negative to positive charge.Conclusion In the three kinds of polymer/adenovirus compounds,the cytotoxicity of the compound is positively correlated with the polymer molecular weight and concentration.The greater molecular weight the polymer as well as the higher the concentration are,the higher the cytotoxicity is.

Inflammatory bowel disease(IBD),the precise etiology of which remains unknown,is comprised of two forms of chronic intestinal inflammation,ulcerative colitis and Crohn's disease.Recent researches suggest that angiopoiesis plays an important role in the development of IBD.Many cytokines can enhance angiopoiesis,such as fibroblast growth factor-2,interleukin-8,platelet-derived growth factor,transforming growth factor-? and particularly vascular endothelial growth factor.This review summarizes the angiopoietic factors correlated with IBD.