OBJECTIVETo explore surgical management and the effects of surgical treatment of severely comminuted fractures of humeral intercondylar through approach of osteotomy olecranon with double-plate internal fixation.

METHODSThirty patients of severely comminuted fractures of humeral intercondylar were treated by double-plate internal fixation through osteotomy olecranon. There were 19 males and 11 females, with an average age of 42.3 years ranging from 21 to 62. According to AO/ASIF classification, there were 17 cases of type C2 and 13 of C3. Twenty-one cases were applied with reconstructive plate medially and laterally, 9 cases were applied with reconstructive plate laterally and 1/3 tubular plate medially.

RESULTSAll patients were followed-up for 6 months to 3 years (means 1.5 years). The complications such as bone defect, ossifying myositis, tardive ulnar nerve compression, internal fixation fail were no occurenced. According to Aitken-Rorabeck scale the function of the elbow showed excellent in 16 cases, good in 10, fair in 2 and poor in 2.

CONCLUSIONAnatomical reduction surgically reconstructed stabilization of the elbow and early active rehabilitation are crucial factors of functional rehabilitation of the elbow. The technique of osteotomy olecranon with double-plate internal fixation for treatment of severely comminuted fractures of humeral intercondylar is a good approach.

Adult ; Female ; Fracture Fixation, Internal ; methods ; Fractures, Comminuted ; therapy ; Humans ; Humerus ; surgery ; Male ; Middle Aged ; Osteotomy ; methods ; Rehabilitation ; Treatment Outcome ; Young Adult

Objective: To observe the inhibitory effects of biotic royal jelly on the ascitic hepatoma cell H22. Methods: Mice bearing H22 tumor were fed on different types of royal jelly: No.1, 2 and 3. Their anti-tumor effects were observed in vivo. The general royal jelly and normal saline were observed as control. Results: Among these biotic royal jelly, the biotic royal jelly No.1 showed obvious tumor-inhibiting and survival-prolonging effects. In addition, it increased the number of WBC and augmented the amount of IL-2 and IFN-γ; the pathological study also indicated the denaturing and necrosis of most tumor cells with nuclei constraining and cell membrane rupturing, and large amount of lymphocytes and plasmacytes infiltrating around the mass. Conclusion: The No.1 biotic royal jelly has obvious anti-tumor effect, and it may take effects by inhibiting or killing tumor cells and improving the immunity of the host.

OBJECTIVETo compare the accuracy of preoperative T staging of gastric cancer by oral and intravenous contrast-enhanced gastric ultrasonography.

METHODSOne hundred and forty three patients who had been diagnosed as gastric cancer by endoscopic biopsy and confirmed by pathology after operation were examined by oral and intravenous contrast-enhanced gastric ultrasonography, and they were divided into satisfied group and non-satisfied group according to the 2-D image quality of lesion. The results were compared with postoperative pathologic findings.

RESULTSAll the patients with gastric cancer presented regional gastric wall thickening. Among them, 117 cases were clearly presented with good image quality. The remaining 26 cases were presented with vague profile, the ulcerative surface of lesion was filled with hyperechogenicity combined with rear shadow. The accuracy of oral contrast-enhanced ultrasonography in determining the T stage of gastric cancer was 74.1%. The accuracy in satisfied group and non-satisfied group was 78.6% and 53.8%, respectively. The enhancement pattern of 143 cases was showed as hyperenhancement during the arterial phase and hypoenhancement during the portal phase in DCUS. The accuracy of double contrast-enhanced ultrasongraphy in determining the T stage of gastric cancer was 86.7%, but the accuracy in satisfied group and non-satisfied group was 88.9% and 76.9%, respectively. There was a significant difference between the two methods (χ(2) = 9.031, P < 0.01).

CONCLUSIONDCUS is more accurate than oral contrast-enhanced ultrasonography as a useful diagnostic method for preoperative T staging of gastric cancer.

Adult ; Aged ; Contrast Media ; Female ; Humans ; Male ; Middle Aged ; Neoplasm Staging ; methods ; Preoperative Period ; Stomach Neoplasms ; diagnostic imaging ; pathology ; Ultrasonography ; methods

OBJECTIVETo investigate the effect of COX1 and COX2 on angiogenesis and endothelial progenitor cell mobilization in rats with experimental myocardial infarction (MI).

METHODSThe rats were randomly divided into 3 groups: MI group, MI plus rofecoxib group and MI plus valeryl salicylate group. At the 7th day after operation, circulating EPCs, plasma VEGF and HIF-1alpha mRNA of ischemic myocardium were measured. At the 28th day post operation, capillary densities were also measured in ischemic myocardium.

RESULTCompared with the MI group and the MI plus valeryl salicylate group, circulating EPCs, plasma VEGF, HIF-1alpha mRNA and capillary densities of ischemic myocardium were all decreased in MI plus rofecoxib group.

CONCLUSIONThe present study revealed that COX2 play an important role with angiogenesis and endothelial progenitor cell mobilization in rat with experimental MI by modulating expression of VEGF and HIF-1alpha.

Animals ; Cyclooxygenase 2 ; biosynthesis ; Cyclooxygenase Inhibitors ; pharmacology ; Endothelial Cells ; cytology ; Female ; Hypoxia-Inducible Factor 1, alpha Subunit ; biosynthesis ; Myocardial Infarction ; drug therapy ; pathology ; Neovascularization, Physiologic ; drug effects ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; metabolism ; Vascular Endothelial Growth Factor A ; blood

OBJECTIVETo investigate the diagnostic value of contrast-enhanced ultrasonography (CEUS) in preoperative T-staging of gastric cancer.

METHODSA sulfur hexafluoride-filled microbubble ultrasound contrast agent and a continuous real-time imaging technique of contrast pulse sequencing were used. Normal gastric wall was examined by CEUS in 8 healthy volunteers and the results were compared with the findings on multislice computed tomography. Sixty-two patients with gastric cancer proved by biopsies who received preoperative CEUS examination were involved in this study, and the CEUS result was compared with postoperative pathological findings.

RESULTSThe normal gastric wall presented a one-layer structure in the portal venous phase and a three-layer structure in the arterial and equilibrium phase including a slightly hyper-enhanced inner layer, a hypo-enhanced intermediate layer, and a markedly hyper-enhanced outer layer, which corresponded histologically to the mucosal, submucosal, and muscular-serosal layer, respectively. The accuracy of transabdominal ultrasonography and CEUS in determining the T stage of gastric cancer was 72.9% and 88.1% respectively, and the difference was statistically significant (chi(2)=4.37, P=0.036).

CONCLUSIONSCEUS shows the normal gastric wall as a one- or a three-layer structure, which provides a theory base for CEUS in preoperative T-staging of gastric cancer. CEUS is a useful diagnostic method for preoperative T-staging of gastric cancer.

Adult ; Aged ; Contrast Media ; Female ; Humans ; Male ; Middle Aged ; Neoplasm Staging ; methods ; Stomach Neoplasms ; diagnostic imaging ; pathology ; Sulfur Hexafluoride ; Ultrasonography ; methods

Virus is a kind of simple non-cellular structure microorganism which contains only one nucleic acid and protein shell. With the development of molecular biology, as an important biological resource, the preservation value of microbial strains (viruses) has been paid more and more attention. As a cheap, simple and time-saving method, low temperature preservation has been widely used in the preservation of microorganisms, compared with the passage method and the drying method which is easy to be contaminated by other microorganisms. However, there are few and no in-depth studies on low temperature storage of viruses. Based on this, according to the characteristics of the virus specific cell, the viruses can be divided into animal virus (phage), plant virus and bacterial virus (phages). This article reviews preservation temperature, low temperature protective agent, and other factors on the influence of different kinds of virus effect of low temperature preservation, preservation technology problems and development trend, in order to further improve the microbial status of low temperature preservation research to provide the reference.

OBJECTIVETo evaluate the efficacy and safety of capecitabine as first-line therapy in patients with advanced and recurrent colorectal cancer.

METHODSFrom December 2000 to November 2001, sixty patients with advanced and recurrent colorectal cancer received first-line capecitabine treatment given at a dose of 1250 mg/m(2) twice daily, on days 1 - 14 every 21 days. At least 2 cycles were administered.

RESULTSThe overall response rate was 23.3% with 14 PR, 24 SD (40.0%) and 15 PD. The median survival time was 14.7 months. The survival rate was 63.9% at 12-months and 33.4% at 24-months. Grade III-IV adverse effects were diarrhea in 4 patients (6.6%), anemia in 2 (3.3%) and hand-foot syndrome (HFS) in 1 (1.7%); Grade I-II adverse effects were hyperpigmentation in 20 (33.3%), HFS in 18 (30.0%) and diarrhea in 10 (16.7%).

CONCLUSIONCapecitabine is an efficacious and better-tolerated alternative treatment for the patients with advanced and recurrent colorectal cancer.

Adult ; Aged ; Antimetabolites, Antineoplastic ; therapeutic use ; Capecitabine ; Colorectal Neoplasms ; drug therapy ; mortality ; Deoxycytidine ; adverse effects ; analogs & derivatives ; therapeutic use ; Female ; Fluorouracil ; analogs & derivatives ; Humans ; Male ; Middle Aged ; Survival Rate