PURPOSE: Human tooth proteins are highly heterogeneous, comprising diverse proteins derived from a number of genes. The attempts to identify protein for activity of tooth matrix proteins have been defied by several factors. First, the amount of proteins within teeth is very small relative to many extracellular matrix proteins of other tissues. Second, the bioassay system is tedious and needed for long time. Therefore we tried to find easy techniques, which increase the product rate, and an assay of small proteins, with which amino acid sequence is possible without additional procedures. Materials and METHODS: Total protein were extracted from 300 g enamel removed teeth and 600 g teeth with 4 mol/L guanidine HCl and purified by gel chromatography. Aliquot of proteins was implanted into muscle pouches in Sprague-Dawley rats for bioassay. By SDS-PAGE and membrane blotting, molecular weight of each protein was estimated and a partial amino acid sequence was obtained. Each fraction blotted on the membrane was cut out and inserted in rat ectopic model. RESULTS: In dissociative method, total tooth proteins were obtained 1mg/ml from enamel removed teeth and 3.5 mg/ml from teeth. In SDS-PAGE, four clear bands at the sites corresponding to 66, 40, 20 and 18 kD. Especially The 66 kD band was clearly exhibited. Amino acid sequencing from tooth could be possible using PVDF membrane blotting technique. In amino acid sequencing, 66 kD protein was identified as albumin. CONCLUSION: Compared with conventional method for extraction of teeth protein and bioassay of proteins, the methods in this study were easy, time-saving and more productive technique. The matured tooth proteins omitting additional procedure of mechanical removal of enamel were simply analyzed using blotted PVDF membrane. This method seems to make a contribution as a technique for bioassay and amino acid sequencing of protein.
Amino Acid Sequence ; Animals ; Biological Assay* ; Chromatography, Gel ; Dental Enamel ; Electrophoresis, Polyacrylamide Gel ; Extracellular Matrix Proteins ; Guanidine ; Humans* ; Membranes* ; Molecular Weight ; Rats ; Rats, Sprague-Dawley ; Sequence Analysis, Protein ; Tooth*

The purpose of this study was to evaluate the stability and efficacy of biologic membrane made of freeze-dried cartilage as a barrier to facilitate guided bone regeneration in experimental non-healing bone defects in the rat mandible. Nine adult Sprague-Dawley rats (400-500g) were used in experiment. 5.0mm in diameter were created on the mandibular angle area by means of slow-speed trephine drill. In microscopic examination, dynamic immature bone forming at 2 weeks and its calcification at 4 weeks were observed. The membrane made of lyophilized cartilage taken from human costal cartilage seems to be very effective for guided bone regeneration as a biologic membrane and the scaffold for attachment of cells or local drug delivery system of growth factor, which may meet the ideal requirement of a barrier membrane and graft materials.
Adult ; Animals ; Bone Regeneration* ; Cartilage ; Drug Delivery Systems ; Humans ; Mandible ; Membranes* ; Rats ; Rats, Sprague-Dawley ; Transplants

The purpose of this study was to evaluate the resistant force of medial pterygoid muscles against the mandibular advancement and distraction to anterior, and inquire into the relationship between medial pterygoid muscles and cephalometric variables. Sixty six patients with class III malocclusion underwent bilateral sagittal splitting of ramus with intraoralvertico-sagittal ramus osteotomy for mandibular set-back. The spring scale was used to measure the resistance of medial pterygoid muscles after splitting of ramus. Skeletaldental cephalometric analysis was made and statistic package was used for correlation between resistance and cephalometric variables. The resistant force of the right medial pterygoid muscle was greater than the left one in Koreans with class III malocclusion, and the force had a linear regression relationship with facial depth. The results suggested that facial depth has significant correlation with the resistance of medial pterygoid muscle, which can be acquired from patient's cephalometric analysis.
Humans ; Linear Models ; Malocclusion ; Mandibular Advancement ; Osteotomy ; Pterygoid Muscles*

Coloring Agents ; Cytomegalovirus ; Endothelial Cells ; Humans ; Intranuclear Inclusion Bodies ; Male ; Methenamine ; Mouth ; Oral Manifestations ; Stomatitis ; Tongue ; Ulcer

Esthetics ; Facial Asymmetry ; Genioplasty ; Humans ; Jaw ; Orthognathic Surgery ; Osteotomy ; Rhinoplasty ; Seoul ; Surgery, Oral ; Surgery, Plastic

BACKGROUND: This is a retrospective study of 42 pregnancies from 33 women with prosthetic heart valves who were on anticoagulation regimen prior to or during their pregnancy. MATERIAL AND METHOD: Of the 17 women with bioprosthesis, 15 had 21 pregnancies following cessation of the anticoagulation therapy which resulted in the delivery of 20 healthy babies and 1 abortion. Remaining 2 had 3 pregnancies maintained with heparin, resulting in 2 healthy babies and 1 spontaneous abortion. RESULT: Among 16 women with mechanical heart valves, there were 7 pregnancies during which warfarin was used and this was associated with 4 fetal wastages(2 therapeutic abortion, 1 spontaneous abortion and 1 stillbirth with cerebral hemorrhage). However, in pregnancies where heparin was used, there was no fetal wastage. A patient who did not take anticoagulant for the first trimester and took warfarin for the remaining period and a patient who did not take anticoagulant during pregnancy delivered normal babies. There was an other fetal wastage in a patient on anti-platelet therapy for the first trimester and warfarin therapy for the remaining periods. There was 1 minor petechial complication in a heparin administered group. CONCLUSION: The study indicates that woman with bioprosthetic heart valves can go through pregnancy without undue risks or complications. On the other hand, the use of warfarin during pregnancy in women with mechanical heart valves, was shown to be associated with unacceptable high risk for the fetus. However, in the same group of women, judicious use of heparin during pregnancy was accompanied by a much reduced risk. The safety and adequate therapeutic range of heparin usage under such circumstances are subject to further studies.
Abortion, Spontaneous ; Abortion, Therapeutic ; Bioprosthesis ; Female ; Fetus ; Hand ; Heart Valve Prosthesis ; Heart Valves* ; Heart* ; Heparin ; Humans ; Pregnancy Trimester, First ; Pregnancy* ; Retrospective Studies ; Stillbirth ; Warfarin

PURPOSE: To describe systemic complications of unconscious patients' survival six hours following cardiopulmonary resuscitation (CPR), and to evaluate the relationship between several variables and death. METHODS: We conducted a retrospective analysis of 153 cases of non-traumatic cardiac arrest that received CPR in a tertiary emergency department from April 1997 to June 2007. We carried out analyses on survival cases, and studied general characteristics and systemic complications to ascertain relationships between the survival and death groups. RESULTS: Sixty of 153 patients initially survived, but the survival rate quickly decreased to 30.1% within the first three days and then decreased slowly thereafter. In the 153 patients, hyperamylasemia occurred in 101 patients (66.0%), acute liver injury in 75(49.0%), sepsis in 67(43.8%), seizure and refractory shock in 66(43.1%), pneumonia in 65(42.5%), acute renal failure (ARF) in 64(41.8%), acute respiratory distress syndrome (ARDS) in 30(19.6%), and disseminated intravascular coagulation (DIC) in 26(17.0%). Of nine types of complications encountered, the incidences of hyperamylasemia, ARDS, ARF, refractory shock and DIC were significantly higher in patients who died than in those who survived (73.1% vs 55.0%, 28.0% vs 6.7%, 54.8% vs 21.7%, 66.7% vs 6.7%, and 23.7% vs 6.7%, respectively). CONCLUSION: Systemic complications occurred with high frequency in comatose survivors after cardiopulmonary resuscitation. Hyperamylasemia, ARDS, ARF, refractory shock and DIC were significantly associated with death.
Acute Kidney Injury ; Cardiopulmonary Resuscitation ; Coma ; Dacarbazine ; Disseminated Intravascular Coagulation ; Emergencies ; Heart Arrest ; Humans ; Hyperamylasemia ; Incidence ; Liver ; Pneumonia ; Respiratory Distress Syndrome, Adult ; Retrospective Studies ; Seizures ; Sepsis ; Shock ; Survival Analysis ; Survival Rate ; Survivors ; Unconscious (Psychology)

The incident of false aneurysm of internal maxillary artery in the oral and maxillofacial region is known to be very rare. One case of false aneurysm in the internal maxillary artery is presented, which was experienced in our department. The etiology of this case is regarded as the mandibular condyle fracture, and/or iatrogenic trauma during open reduction. Clinically, there were systolic bruit on auscultation, pulsation and massive bleeding during operation. The lesion was finally diagnosed with angiography and treated by embolization procedure. False aneurysm can cause so massive bleeding as to threat the life of the patients. Therefore accurate diagnosis and treatment is very important. Angiography enables the solid diagnosis for the clinical one. And as the embolization has many advantages over the ligation, it can be a good treatment method.
Aneurysm, False* ; Angiography ; Auscultation ; Diagnosis ; Hemorrhage ; Humans ; Ligation ; Mandibular Condyle ; Maxillary Artery*

Ameloblastomas are generally considered to be benign tumors of odontogenic epithelial origin with high local recurrence. Rarely ameloblastomas exhibit malignant behavior with development of metastases. In this report, we present a 19-year-old woman with ameloblastoma in the right ascending ramus and multiple recurrences. Eleven years after first therapy, ameloblastoma metastasized to ipsilateral submandibular lymph node. We also review literature about cause, treatment and work-up of malignant ameloblastoma.
Ameloblastoma* ; Female ; Humans ; Lymph Nodes* ; Mandible* ; Neoplasm Metastasis* ; Recurrence ; Young Adult

OBJECTIVE: Acute Renal Failure is a clinical syndrome characterized by a sudden decrease in renal function which was previously normal. Despite advances in medical care, prognosis in ARF is variable according to the influence of demographic factors, severity of ARF, nature of disease causing ARF, coexisting disease, treatments applied, and complications. We studied the recent changes of clinical feature of ARF. METHODS: We studied retrospectively 245 patients with ARF who had been hospitalized at Kyung Hee University Hospital between February 1988 and March 1993. RESULTS: 1) Male to female sex ratio was 1.8 : 1, and the incidence was high in above fifth decade (67.8%). 2) Acute renal failure was classified, according to clinical background, into medical group 79.6% (195 cases) and surgical group 20.4% (50 cases), and oliguric group 40.8% (100 cases) and non-oliguric group 59.2% (145 cases). 3) Acute renal failure due to medical causes included ARF by hemorrhagic fever with renal syndrome (25.6%), drugs and chemicals (17.9%), sepsis (17.4%) and systemic infection (7.7%) etc. ARF due to surgical causes included ARF by multiple trauma (34%), various surgical procedures (30%), surgical sepsis (14%), burn (12%) etc. 4) During admission, the expired patients had more severe biochemical and clinical characteristics including high BUN and serum potassium (p<0.01), lower serum albumin (p<0,01) than those of survivor. 5) Infections as the cause of ARF were 107 cases (43.7%), which included hemorrhagic fever with renal syndrome 50 cases, sepsis 31 cases, urinary tract infection 7 cases and respiratory tract infection 6 cases etc. The most common infecting organism was Hantavizus (50.5%). There was a greater number of gram-negative organisms than gram-positive organisms (34.1% vs 9.9%). 6) The overall mortality rate in patients with ARF was 31.4Fo. The presumptive causes of death were underlying disease (59.7%) such as sepsis, acute poisoning, cardiogenic and hypovolemic shock, and respiratory failure (14.3%), hyperkalemia (9.1%), pulmonary edeme (6.5%), and metabolic aidosis (2.6%) in order of frequency. 7) The highest mortality rate was 42.6% in patients above 50 years old. Mortality rate in patients with ARF due to surgical causes (52.0%) was significantly high than that of medical causes (26.2%) (p<0.05). Among the expired patients, oliguric group was 72.7%. In conclusion, there have been major trends in the clinical features of acute renal failure in this study. Especially, significant increase in the number of elderly patients, non-oliguric patients, and medical causes such as hemarrhagic fever with renal syndrome or sepsis were observed. Survival rate significantly decreased with increasing age, in acute renal failure by surgical causes, in oligurie patients, and in the presence of complicating factors such as sepsis or shock.
Acute Kidney Injury* ; Aged ; Burns ; Cause of Death ; Demography ; Female ; Fever ; Hemorrhagic Fever with Renal Syndrome ; Humans ; Hyperkalemia ; Incidence ; Male ; Middle Aged ; Mortality ; Multiple Trauma ; Poisoning ; Potassium ; Prognosis ; Respiratory Insufficiency ; Respiratory Tract Infections ; Retrospective Studies ; Sepsis ; Serum Albumin ; Sex Ratio ; Shock ; Survival Rate ; Survivors ; Urinary Tract Infections