An elicitor from fungus Mycenae sp. enhanced the extracellular pH of protocorms of Dendrobium candidum in two stages and also inspired the activities of PAL, POD and LOX. The different elicitors were different in enhancing the pH. The activities of PAL and POD ascended twice after elicitors were applied. The protocorms treated twice by elicitor had the higher PAL activity.

OBJECTIVEThe suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth.

METHODThe growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied.

RESULT AND CONCLUSIONThe diluted MS medium promotes the growth of protocorms but inhibit differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.

Carbohydrates ; pharmacology ; Culture Media ; Dendrobium ; growth & development ; Fungi ; physiology ; Hydrogen-Ion Concentration ; Plant Growth Regulators ; pharmacology ; Plants, Medicinal ; growth & development ; Symbiosis ; physiology ; Tissue Culture Techniques