1.Recovery and Adsorption Rate of Murine Norovirus Using NanoCeram(R) Filters.
Yun Hee KIM ; Seok Jea JANG ; Ji Youn PARK ; Jung Hwan OH ; Geun Su KIM ; Tae Seung KIM ; Oh Sang KWON ; Jin Seok HAN ; Won Hwa JHEONG
Journal of Bacteriology and Virology 2011;41(1):55-61
This study investigated the recovery and absorption rates of murine norovirus, a surrogate for human norovirus, by using NanoCeram(R) filters which served as a tool for recovering viruses. In the study, two types of NanoCeram(R) filters were employed: one was a cartridge type and the other was a disc type (phi 47 mm) whose surface area is 75 times smaller than the cartridge type. The analytical method was the real-time reverse transcription-polymerase chain reaction (RT-PCR). The study found that the average recovery rates of the cartridge type and the disc type were 30.9% and 29.5% respectively. Since these two rates were very close to each other, the adsorption rate of the cartridge type could be predicted with the disc type. Analyzing recovery and absorption rates of the disc type based on different filtered volumes showed that when the volume increased from 0.5 L to 20 L, the average recovery rate rose from 14.78% to 30.41 %, while the average absorption rate dropped from 56.33% to 10.48%. The increase in turbidity from less than 1 NTU to less than 3 NTU raised the average recovery rate from 47.23% to 82.84%.
Absorption
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Adsorption
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Humans
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Norovirus
2.Follow control of upper limb rehabilitation training based on Kinect and NAO robot.
Xiaoguang LIU ; Simin LI ; Tie LIANG ; Jun LI ; Cunguang LOU ; Hongrui WANG ; Xiuling LIU
Journal of Biomedical Engineering 2022;39(6):1189-1198
Gesture imitation is a common rehabilitation strategy in limb rehabilitation training. In traditional rehabilitation training, patients need to complete training actions under the guidance of rehabilitation physicians. However, due to the limited resources of the hospital, it cannot meet the training and guidance needs of all patients. In this paper, we proposed a following control method based on Kinect and NAO robot for the gesture imitation task in rehabilitation training. The method realized the joint angles mapping from Kinect coordination to NAO robot coordination through inverse kinematics algorithm. Aiming at the deflection angle estimation problem of the elbow joint, a virtual space plane was constructed and realized the accurate estimation of deflection angle. Finally, a comparative experiment for deflection angle of the elbow joint angle was conducted. The experimental results showed that the root mean square error of the angle estimation value of this method in right elbow transverse deflection and vertical deflection directions was 2.734° and 2.159°, respectively. It demonstrates that the method can follow the human movement in real time and stably using the NAO robot to show the rehabilitation training program for patients.
Humans
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Upper Extremity
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Robotics/methods*
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Stroke Rehabilitation/methods*
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Elbow Joint
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Physical Therapy Modalities
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Biomechanical Phenomena
3.Dynamic investigation of tibial biomechanical property endured persistent intensive stress.
Cheng LI ; Guoping LI ; Fuxing PEI
Journal of Biomedical Engineering 2007;24(2):324-328
For investigating the changes in continuous biomechanical nature of bone bearing intensive stress in vivo, we adopted the animal model of persistent intensive stress. Thirty-seven rabbits were involved in the experiment; they were randomly divided into control group (5 rabbits) and trained group (32 rabbits). All animals were forced to jump and run about 300 times everyday in the electric stimulation cage so as to simulate the persistent intensive stress on tibias for different periods. Subsequently, all animals were sacrificed at different times (1-11 weeks), and all tibias of them were collected for biochemical investigation. By torsion destroy test, the experiment showed that the changes of biomechanical nature appeared obviously in a period of 6 weeks or so in the experiment. Furthermore, during the 2nd-3rd week of each period, the biomechanical indexes decreased strikingly; the abilities of deformation resistant dropped, the twisting rigidity decreased (69.7% lower than control, P< 0.01), the flexible index inereased (203.2% higher than control, P<0.05), the angle of twisting destroy increased (102.9% greater than control, P< 0.05). And the tibial mechanical strength declined too; the destroy torsion, energy absorption and energy absorb density decreased (most of them being 50% lower than control, even accounting for 34.5% of control; P< 0.05 or P< 0.01). We noticed that the biomechanical properties of bone endured persistent intensive stress presented periodicity and the period of change in bone biomechanical nature covered about 6 weeks in the experiment. Therefore, we have ground to conclude that a weakness period did exist when rabbit tibias endured 2-3 weeks persistent intensive stress, and such weakness did exist at the 2nd-3rd week of each training period.
Adaptation, Physiological
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Animals
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Biomechanical Phenomena
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Bone Density
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Male
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Physical Conditioning, Animal
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Rabbits
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Random Allocation
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Stress, Mechanical
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Tibia
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physiology
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Time Factors
4.A Standing Balance Evaluation Method Based on Largest Lyapunov Exponent.
Kun LIU ; Hongrui WANG ; Jinzhuang XIAO ; Qing ZHAO
Journal of Biomedical Engineering 2015;32(6):1212-1216
In order to evaluate the ability of human standing balance scientifically, we in this study proposed a new evaluation method based on the chaos nonlinear analysis theory. In this method, a sinusoidal acceleration stimulus in forward/backward direction was forced under the subjects' feet, which was supplied by a motion platform. In addition, three acceleration sensors, which were fixed to the shoulder, hip and knee of each subject, were applied to capture the balance adjustment dynamic data. Through reconstructing the system phase space, we calculated the largest Lyapunov exponent (LLE) of the dynamic data of subjects' different segments, then used the sum of the squares of the difference between each LLE (SSDLLE) as the balance capabilities evaluation index. Finally, 20 subjects' indexes were calculated, and compared with evaluation results of existing methods. The results showed that the SSDLLE were more in line with the subjects' performance during the experiment, and it could measure the body's balance ability to some extent. Moreover, the results also illustrated that balance level was determined by the coordinate ability of various joints, and there might be more balance control strategy in the process of maintaining balance.
Acceleration
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Biomechanical Phenomena
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Humans
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Models, Biological
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Motion
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Nonlinear Dynamics
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Postural Balance
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physiology
5.Progress of research on human tolerance to impact acceleration.
Bingkun LIU ; Honglei MA ; Shizhong JIANG
Journal of Biomedical Engineering 2010;27(2):444-447
Impact acceleration is one of the factors to which human body is exposed in aerospace exploring. When the impact level is greater than human tolerance, it usually results in human injuries which may be fatal. Therefore, in order to reduce or avert the risk of serious injury from crash impact, human tolerance to impact acceleration is a crucial consideration in aircraft since the beginning of aviation. The study on human tolerance to impact acceleration has become a cynosure in the realm of modern biomechanics. So this paper reviews the progress of the researches.
Acceleration
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adverse effects
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Accidents, Aviation
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Adaptation, Physiological
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Aerospace Medicine
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Biomechanical Phenomena
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Computer Simulation
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Humans
6.Effects of pre-treatment on Cu2+ absorption of Penicillium janthinellum strain GXCR.
Xiaoting HUANG ; Changbin SUN ; Xiaoling CHEN ; Huijuan QIN ; Mei HU ; Yuan YUAN ; Youzhi LI
Chinese Journal of Biotechnology 2009;25(1):76-83
In order to effectively increase capacity of Cu2+ absorption by Penicillium from Cu2+-containing aqueous solution and to study the mechanisms of absorption, effects of eight pre-treatment methods on Cu2+ absorption of Penicillium janthinellum strain GXCR were compared. The results showed that the efficiency of Cu2+ absorption obviously increased through pre-treatment by homogenization, homogenization-basification (NaOH), oven dry (80 degrees C), homogenization-salinification (NaCl), homogenization-detergent and homogenization-polarization (C2H6SO), but significantly decreased after acidification pretreatment with H2SO4. In comparison with the previous reports, the pretreatment in a homogenization-NaOH way could more efficiently enhance the Cu2+ absorption capacity of this fungus. Homogenization-basification (0.5 mol/L NaOH) increased Cu2+ biosorption by 47.95%. The Cu2+ absorption of the mycelia treated by homogenization-basification followed Langmuir isotherm equation, suggesting a surface absorption process. After four cycles of absorption-desorption, mycelia pretreated by homogenization-alkalization still had 70.82% of Cu2+ biosorption efficiency. Infrared reflectance analysis indicated that alkalization treatment made marked effects on molecular groups of C-H, C=O, and C=O in COOH on the mycelial surfaces, and -OH was a key Cu2+-binding group. It is therefore suggested that the Cu2+ absorption by the GXCR is likely to be a chemical absorption process through Cu2+ binding with -OH group on the mycelia.
Adsorption
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Biodegradation, Environmental
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Copper
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metabolism
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Penicillium
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metabolism
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Physical Phenomena
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Sodium Hydroxide
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chemistry
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Waste Disposal, Fluid
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methods
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Water Pollutants, Chemical
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metabolism
7.Development of artificial disc nucleus materials (semicrystalline polyvinyl alcohol hydrogel elastomers).
Zhengqiu GU ; Yuanzheng MA ; Jin GAO ; Jiantao LIU ; Yong LI
Journal of Biomedical Engineering 2004;21(3):347-349
For the purpose of developing of disc nucleus replacement (semicrystalline PVA hydrogel elastomers) and explorating the possibility of clinical application, in this study,the aqueous PVA solution froze at -20 degrees C for 6-12 h, and then thawed at room temperature for 1-2 h. The same process was repeated 1-3 times. After the specimen was dehydrated in vacuum, a kind of artificial disc nucleus materials (semicrystalline PVA hydrogel elastomers ) was formed. DSC and mechanical tests were conducted to investigate the influence of aqueous PVA solution concentration, of dehydrating in vacuum, and of gamma-ray irradiating upon the degree of crystallity and mechanical properties of PVA hydrogel elastomers.
Biocompatible Materials
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Biomechanical Phenomena
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Cartilage, Articular
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Chemical Phenomena
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Chemistry, Physical
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Crystallization
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Elastomers
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Humans
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Intervertebral Disc
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surgery
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Materials Testing
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Polyvinyl Alcohol
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chemical synthesis
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chemistry
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Prostheses and Implants
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Prosthesis Design
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Tensile Strength
8.The sEMG-force relationship during ramp contractions of biceps brachii in non-fatigue exercises.
Chao SONG ; Jian WANG ; Lan-ping LOU
Chinese Journal of Applied Physiology 2010;26(1):97-101
OBJECTIVETo evaluate the capability of C(N) and determinism% (DET%) together with the linear indices, we detected the surface electromyography (sEMG) signal changes during ramp contractions of biceps brachii.
METHODS5 seconds sEMG signals of 11 male volunteers were recorded to analyze its changes of the linear indices AEMG, mean power frequency(MPF), MF and the non-linear indices C (N), DET%.
RESULTSAverage electromyography (AEMG) inclined linearly from 112.14 microV to 1277.18 microV while DET% displayed an decrease from 74.95 to 46.7 during the 5 voluntary contractions. The spectrum parameters MPF/MF and C(N) leveled off with the increasing of the force.
CONCLUSIONThe linear index AEMG inclined linearly while MPF and MF leveled off with the increasing of the force. The non-linear index DET% declined while C(N) leveled off during the 5 seconds ramp contractions.
Adolescent ; Adult ; Arm ; Biomechanical Phenomena ; Electromyography ; Humans ; Male ; Muscle Contraction ; physiology ; Muscle Fatigue ; physiology ; Muscle, Skeletal ; physiology ; Physical Endurance ; Young Adult
9.Research progresses on ergonomics assessment and measurement methods for push-pull behavior.
Yan ZHAO ; Dongxu LI ; Shengpeng GUO
Journal of Biomedical Engineering 2011;28(5):1052-1056
Pushing and pulling (P&P) is a common operating mode of operator's physical works, and plays an important role in evaluation of human behavior health and operation performance. At present, there are many research methods of P&P, and this article is a state-of-art review of the classification of P&P research methods, the various impact factors in P&P program, technical details of internal/external P&P force measurement and evaluation, the limitation of current research methods and the future developments in the ergonomics field.
Biomechanical Phenomena
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Ergonomics
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methods
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Hand Strength
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physiology
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Humans
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Low Back Pain
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prevention & control
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Physical Exertion
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Posture
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physiology
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Task Performance and Analysis
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Weight-Bearing
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physiology
10.Bilateral Femoral Derotatioal Osteotomy in Spastic Diplegia: Outcome Assessment of Single Stage Multilevel Surgery.
Chin Youb CHUNG ; Moon Seok PARK ; Byung Wook SONG ; In Ho CHOI ; Tae Joon CHO ; Won Joon YOO
The Journal of the Korean Orthopaedic Association 2007;42(6):711-717
PURPOSE: To evaluate the outcome of multilevel surgery including femoral derotational osteotomy, and analyze the effect of bilateral femoral derotational osteotomy on the gait of spastic diplegia. MATERIALS AND METHODS: The medical records of 26 spastic diplegics, who had undergone a bilateral intertrochanteric femoral derotational osteotomy, bilateral distal hamstring lengthening, bilateral rectus femoris transfer, and bilateral heel cord lengthening, were evaluated. There were 16 boys and 10 girls with a mean age of 7.6 years. The pre- and post-operative gait analysis, functional assessment score, and physical examination were archived and analyzed. RESULTS: In the physical examination, the femoral anteversion, hip flexion contracture, and popliteal angle decreased significantly, while the ankle range of dorsiflexion increased significantly. In three dimensional gait analysis, the cadence remained constant while the walking speed improved significantly. In transverse plane kinematics, the mean pelvic rotation did not show any difference but foot progression angle fell into the normal range after surgery. In the sagittal plane, the maximal hip extension and H3 power generation improved significantly, while the mean anterior pelvic tilt decreased significantly. The functional assessment score improved from 7 to 9, which was significant. CONCLUSION: The walking ability of spastic diplegia with in-toeing, stiff knee and tip toeing gait improved after single stage multilevel surgery including a femoral derotational osteotomy. Femoral derotation osteotomy without psoas lengthening improved the anterior pelvic tilt despite the distal hamstring lengthening
Ankle
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Biomechanical Phenomena
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Cerebral Palsy*
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Contracture
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Female
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Foot
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Gait
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Heel
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Hip
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Humans
;
Knee
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Medical Records
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Muscle Spasticity*
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Osteotomy*
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Physical Examination
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Quadriceps Muscle
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Reference Values
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Toes
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Walking