The procedure using affinity chromatography for purification of G6PD from rabbit erythrocytes has been developed. This method consists of three following steps: ion exchange chromatography on DEAE - Sephadex A50, precipitation with ammonium sulfate and affinity chromatography on 2'5' ADP - Sepharose 4B. The first and the second step are directly derived from the procedure of NguyÔn H÷u ChÊn. By our method, the accumulative purification of G6PD from rabbit erythrocytes was 10243 fold. A specific activity of 69.66 IU/mg protein and a final yield of 40% were obtained.
Isolation & purification ; Erythrocytes ; Chromatography

PSA is a protein with antigen properties, generated by epicyte of prostate gland, excretes into microtube and then comes mostly into sperma, litter into serum and lymphatic fluid with unclear mechanism. PSA in these are determined by chemicoimmune. The average human serum PSA is 2.5 ng/ml. PSA is specific antigen with cells of prostate-gland and the application of PSA test in diagnostics and monitoring is significant in clinical. Especially, the level of PSA help to early diagnose the prostatic cancer and distinguished diagnose between prostatomegaly and prostatic cancer.
Prostatic Neoplasms ; Antigens ; diagnosis

The hyperlipidemia was usually found out in the community. It can result in some diseases as ischaemic heart disease, cerebralvascular disease, acute myocardial infarction. Some parameters of lipid were studied in women (total cholesterol, high density lipoprotein - cholesterol - HDLC, low density lipoprotein - cholesterol - HDLC, triglyceride and total lipid levels as compared to reference limit. 17 of 97 women had hyperlipidemia (7.39 +/- 3.1 mmol/l), 5 of them elevated total cholesterol (>6.3 mmol/l), 9 of them elevated triglyceride (>2.3 mmol/l) and 3 of them elevated l cholesterol.
Hyperlipidemia ; Myocardial Infarction

The urine parameters for 24h of two people groups were studied. The first group consists of third year students who were living in Ha Noi Medical University (HMU) dormitory. The second group consists of patients with kidney failure who were in C6 department of B¹ch Mai Hospital. The obtained results as follows: (1) the urine parameters for 24 hours of the first group were in normal limits but urine volume decreases, nitrite and nitrate levels increase. Almost of the urine parameters for 24 hours of the second group were abnormal
Kidney Failure ; diagnosis

Background: Obstetric complications always are the risk factors which impact on pregnant women, especially no having care and treatment on time. Objectives: (1) To determine the rate of obstetric complications in some communes of Hoai Duc district, Ha Tay province. (2) To identify some factors which may have impact obstetric complications in the community. Subjects and method: Conducting a cross \ufffd?sectional survey with 363 women randomly chosen for sampling from the list of 1.530 women of reproductive age having terminated their pregnancy dressed for a six \ufffd?month period (from 1 October 2004 to 31 March 2005) in 10 communes of Hoai Duc district, Ha Tay province. Results: The obstetric complications rate was 9.9%. Obstetric complications recorded under the research often had the following forms: hemorrhage (94.4%), infection and pre \ufffd?eclampsia/eclampsia, each of them (2.8%). obstetric complications regularly happened with women of over 40 years old (20%). Those complications were closely linked to vaginal bleeding symptom during pregnant period with p<0.05. Conclusion: Obstetric complication rate was 9.9%, mostly hemorrhage. There was close relation between obstetric complication with vaginal bleeding symptom during pregnant period.
Obstetric Labor Complications ; Residence Characteristics ;

Background: Gestational diabetes is a special form of diabetes mellitus, which is initially diagnosed during gestation. In Vietnam, routine screens for gestational diabetes are not performed. The risk factors of the disease are not taken into account during early screening. Many pregnancies are later diagnosed when complications present themselves. Objective: To determine the prevalence and discover the risk factors for Gestational Diabetes (GD) among pregnant women, who followed up at the Department of Obstetrics and Gynecology, Bach Mai Hospital. Subject and methods: A cross-sectional, prospective study was conducted on 415 pregnant women, who had 24-28 weeks of gestation, followed up at the Department of Obstetrics and Gynecology, Bach Mai Hospital from December, 2006 to April, 2007. All were asked about the risk factors and given an oral glucose tolerance test with 75g of glucose. Results: The prevalence of GD in general population accounted for 7.9%. The prevalence of GD, who had BMI \u226523, family history with 1st degree relative of diabetes and positive glycosuria at gestational age under 24 weeks in subgroups were 25.8%, 37.8% and 55.6%, respectively. None of the pregnant women who had a child weighed \u22654.000g at the previous delivery was diagnosed as GD. However, all thepregnant women, who had history of abnormal glucose tolerance, were GD. Conclusion: Prevalence of GD is increasing. BMI\u226523, family history with 1st degree relative of diabetes and positive glycosuria at age of gestation <24 weeks are considered as high risk factors of GD.
gestational diabetes

Background: In Vietnam, there are a number of studies on the application of ATRA in treating acute promyelocytic leukemia (AML \u2013 M3) but they have still faced with certain difficulties. Objectives: (1). Study PML/RAR alpha fusion gene on the patients diagnosed with AML \u2013 M3. (2). Study the index of hematology of the PML/RAR alpha positive group. Subject and Method: 21 patients with acute promyelocytic leukemia (M3) were studied. All patients were examined with morphology, coagulation and cytogenetic tests and RNA were extracted from leukemic cells and PCR for PML/RAR alpha fusion transcript. Result and conclusion: PML/RAR alpha positive in 67% including 4 patients which were not discovered t(15; 17) by cytogenetic technique. Rates of three subtype (bcr1, bcr2 and bcr3) of PML/RAR alpha were 7 patients (50%), 3 patients (21,5%) and 4 patients (28,5%), respectively. WBC and bone marrow cells of PML/RAR alpha positive group were 5.08+/-3.87 and 155.82+/-106.21. D \u2013 Dimer level was 1954.89+/-1575.28; 93% of patients in the PML/RAR alpha positive group had DIC.
Acute promyelocytic leukemia ; M3 ; PML/RAR alpha

Background: Chromosome mutation type t(8;21) has quite a high frequency in acute myelogenous leukemia, which accounted for about 15% among adult patients. From 2001, the WHO has a new classification for acute myelogenous leukemia based on genetic mutations. Form had AML1/ETO were arranged into genetic mutation group with better prognosis and ability to fully recover after chemotherapy with a high dose of cytarabin. Objective: Study AML1/ETO fusion gene on the patients diagnosed with Acute Myelogenous Leukemia (AML), as well as the clinical features and some haematologic parameters of the AML1/ETO positive group. Subject and methods: 76 patients with AML were treating in the National Institute of Hematology & Blood Transfusion and the Department of Hematology & Blood Transfusion of Bach Mai Hospital from April 2007 to July 2008. These patients were studied for clinical examination, morphology and RNA were extracted from leukemic cells and PCR for AML1/ETO fusion transcript was performed. Results and conclusions: The incidence of AML1/ETO positive in the AML patients was 24%. The incidence of AML1/ETO positive in AML-M2 was 28%. In the AML1/ETO positive group: median age was 26.94+/-9.22; rate of severe anemia, hemorrhage, fever, infection, hepatomegaly, splenomegaly, lymphadenopathy and gum hypertrophy was 44%, 33%, 28%, 11%, 44%, 28%, 17% and 6%, respectively. Median hemoglobin, WBC, platelet, bone marrow cell count, % blast in peripheral blood and in bone marrow was 84.41+/-28.97 g/l, 29.42+/-31.36 g/l, 42.12+/-33.83 g/l, 215.93+/-134.42 g/l, 56.21+/-26.58% and 65.14+/-16.12%, respectively.
acute myelogenous leukemia ; AML1/ETO fusion gene

Introduction: Antiviral resistance has been reported in seasonal influenza A viruses and avian influenza A(H5N1) viruses in Viet Nam, raising concerns about the efficacy of treatment. Methods: We analysed specimens from two sources during the period 2009–2012: influenza-positive samples from influenza-like illness patients at sentinel clinics in northern Viet Nam and isolates from patients with confirmed A(H5N1) infections. Pyrosequencing was used to detect mutations: H275Y [for A(H1N1) and A(H5N1)], E119V [for A(H3N2)] and I117V [for A(H5N1)]. A neuraminidase inhibition assay was used to determine the Inhibitory Concentration 50 (IC50) values for all influenza A and B isolates. Results: There were 341 influenza A positive samples identified; influenza A(H1N1)pdm09 was identified most frequently (n = 215). In 2009, oseltamivir resistance was observed in 100% (19 of 19) of seasonal A(H1N1) isolates and 1.4% (3/215) of A(H1N1)pdm09 isolates. This H275Y mutation was not found in influenza subtypes A(H5N1) or A(H3N2) isolates. Discussion: In Viet Nam, seasonal and A(H5N1) influenza vaccines are not currently available; thus, effective treatment is required. The presence of oseltamivir-resistant viruses is therefore a concern. Active surveillance for oseltamivir resistance among influenza viruses circulating in Viet Nam should be continued.

Background: Bronchoscopy is an essential technique for diagnosis of some lung diseases by taking samples for histological and cytological tests. Objective: To assess the role of transbronchial lung biopsy procedure in diagnosis some parenchymal pulmonary diseases. Subjects and method: A retrospective study conducted in 50 patients with diffuse and localized lung diseases, who were admitted to the Department of Respiratory in Bach Mai Hospital, from January 2003 to December 2005. Results: Transbronchial lung biopsy confirmed the diagnosis in 30 of 50 (60%) cases; with localized malignant lesions (12%), diffuse lung disease (16%), tuberculosis (28%), and cystic fibrosis (4%). Following transbronchial lung biopsy, two patients had pneumothorax (1 patient had chest tube drainage) and five patients had hemoptysis but no of them required further treatment. Conclusions: Transbronchial lung biopsy offer good diagnostic accuracy and low complication rate.
Bronchoscopy/ contraindications ; Lung Diseases/ diagnosis