Process of multiply NS4 gene of hepatitis C virus by PCR technique used with cDNA supplied from ARN. Couple of specific preys for NS4 gene and catalytic enzyme was Deep Vent ADN polymerase. Suitable MgSO4 level to multiply NS4 was 4mM. Fasten prey temperature was factor affecting to specificity of product. Result of electrophoresis showed that the most suitable temperature was 55oC and 57OC. In 200 µM dNTPs level, PCN product was sharp and correct to primary predictable size, in 50 or 100 µM dNTPs level, PCR wasn’t viewed, in 150 µM level, PCR was more blear than using 200 µM level. So dNTPs level was 200 µM
Hepacivirus ; Polymerase Chain Reaction ; Genes ; Enzymes

Study serum of 443 health adults over 20 years old, negativeness with HIV and HBV and serum of 408 health childrens from 2 to 10 years old in Ha noi, Hai Phong and Thanh Hoa. Measles antigen were refined from Vero cell causing infection of Edmonton strain by Gradi extra centrifugal method with Sucrose density 20-60%. HA value of 1/11,024 showed high antigen specificity. 6 bunchs of protein were correllated with specific polypeptid strings of measles virus. After that, technical process and MAC ELISA-IgG were completed. Results: rate of measles immune response in adults was 100%, in which IgG value was high in 24%, moderate in 73.1% and low in 2.8%. Rate of serum IgG value in children was different to adults: positive serum 88%, negative 8.5%. Negative rate showed that the vaccine boosters were necessary
Measles ; Diagnosis ; Immunoglobulin G

The evaluation trial was conducted from August 2004 to March 2005 in the laboratory of the National Institute of Malariolgy, Parasitology and Entomology, Ha Noi. Bioassays with An.dirus and Ae.aegypti (reared mosquitoes) were made according to the WHO method for evaluation of susceptibility and residual effects of the insecticide on the painted and nylon wall surface. Ale lose sprayed at the dose of 30mg ai/m2 were found to produce residual effect for 6-7 months on the painted wall surfaces (mosquito mortalities: 51.1%-67.8%) and for 4-5 months on nylon wall surface (mosquito mortality: 55.6%-67.8%).
Malaria ; Pyrethrins ; Laboratories

2 patients received the microsurgical flap. Both flaps has been living well, the functions were recovered well. The shape of face was relatively equal. The patients received a false maxila and teeth. The moving functions of the leg donated the calcaneal bone were well recovered without influencing the life. The conclusion: the defct of facio-maxillary area is a major lession that should be treated. The technique of microsurgery with using the calcaneal bone flap helps reconstructing the facio-maxillary area.
Therapeutics ; Maxillary Diseases ; Surgical Flaps

This retrospective study was undertaken to characterize the endoscopic and histopathologic pattern of patients with gastro-esophageal reflux with esophagistis. We investigated 58 patients (24 women, 34 men: mean age 44.33 years). The histopathologic pattern was classified in 3 grades: Grade 1: 19 patients, grade 2: 18 patients, grade 3: 21 patients. The sex ratio, age, endoscopic and particular histopathologic pattern was discussed. We conclude that the gastro-esophageal reflux disease is a common complaint in adult population. Despite it is not a severe damage, it can complicate to Barret’s oesophagus, a premalignant lesion of esophageal adenocarcinoma.
Gastroesophageal Reflux ; diagnosis ; Esophagitis

Our study were carried out on 5 patients (3 males, 2 females, the average age was 37.6) in order to evaluate primarily the regime of anti-retrovirus with didanosin and ididavir on 5 HIV patients at some stages of disease. The obvious effectiveness of combination found in the first 3 months, the tolerance was rather good, therefore the drugs can be used for along time. However, the results were satisfactory when using early and the patients were not addicted
HIV ; Acquired Immunodeficiency Syndrome

30 patients with the maxillary ferment tumor treated in the Institute of Face- Maxilla- Tooth during 1994- 1997 participated to a study. The results haves shown that age of disease acquiring were 16-30 years old. The morbidity rate of this disease in female was more frequent than this in male. The early diagnosis of disease impacts significantly on the progress, efficacy of the treatment and opportunity of functional rehebitation after treatment. The tumor in the corner of the mandibular teeth were frequent and related with reaimed teeth. Therefore, the abnormality in this area should be considered. X-ray played very important role in the diagnosis
Maxillary Neoplasms ; Maxilla ; diagnosis

The most vulnerable age group is 31-50 years old (52.3% of patients) with a sex ratio of male/female was 1/1, and the main causes of the great majority of cases are undetermined. The treatment is mainly preservative at a surgical institution and the severe complications must be promptly interventional surgical.
Pancreatitis ; epidemiology ; therapeutics ; surgery

105 pregnant women received the spinal anesthesia by dolargan for the abdominal delivery in Son Tay Hospital during 1-12/2000. The duration of action of the drug, duration of analgesia, efficacy of anesthesia, situation of fetus, influences of the spinal anesthesia on some functions, postoperative time were monitored. The results have shown that the spinal anesthesia is a good method of anesthesia in the abdominal delivery. The care of mother is more convenient than other methods of anesthesia, the cost is lower and the safety for mother and baby is high.
Anesthesia, Spinal ; Cesarean Section

The authors presented 37 cases of thymomas operated at Viet Duc Hospital during 1998-2002. The results: 31 males and 6 females with medium age 46.8. The microscopic features were: medullary thymoma: 1, mixt type: 6, predominantly cortical thymomas: 4, cortical thymomas: 4, squamous cell carcinomas: 11 (4 keratinazing and 7 nonkeratinazing, basaloid carcinomas: 2, clear cell carcinoma: 1, small cell carcinoma: 6, sarcomatous carcinoma: 1 and anaplastic carcinoma: 1. Eleven cases developed the superior vena caval syndrome and most of these indicated the malignancy in the microscopic features. The relations between clinical and pathological aspects were discussed.
Thymoma