1.Guidance on the management of adverse reactions induced by poly(ADP-ribose) polymerase inhibitors.
Of Zhejiang Medical Association SOCIETY OF OBSTETRICS AND GYNECOLOGY ; Of Zhejiang Medical Association SOCIETY OF UROLOGY ; Of Zhejiang Anticancer Association COMMITTEE OF BREAST CANCER ; Of Zhejiang Anticancer Association Preparation COMMITTEE OF CLINICAL PHARMACY ON ONCOLOGY ; Of Zhejiang Society For Mathematical Medicine COMMITTEE OF UROLOGY
Journal of Zhejiang University. Medical sciences 2023;51(6):765-774
The common adverse reactions caused by poly (ADP-ribose) polymerase (PARP) inhibitors include hematological toxicity, gastrointestinal toxicity and fatigue. The main prevention and treatment of hematological toxicity include: regular blood tests, referral to hematology department when routine treatment is ineffective, and being alert of myelodysplastic syndrome/acute myeloid leukemia. The key points to deal with gastrointestinal toxicity include: taking medicine at the right time, light diet, appropriate amount of drinking water, timely symptomatic treatment, prevention of expected nausea and vomiting, and so on. For fatigue, full assessment should be completed before treatment because the causes of fatigue are various; the management includes massage therapy, psychosocial interventions and drugs such as methylphenidate and Panax quinquefolius according to the severity. In addition, niraparib and fluzoparib can cause hypertension, hypertensive crisis and palpitation. Blood pressure and heart rate monitoring, timely symptomatic treatment, and multidisciplinary consultation should be taken if necessary. When cough and dyspnea occur, high resolution CT and bronchoscopy should be performed to exclude pneumonia. If necessary, PARP inhibitors should be stopped, and glucocorticoid and antimicrobial therapy should be given. Finally, more attention should be paid to drug interaction management, patient self-management and regular monitoring to minimize the risk and harm of adverse reactions of PARP inhibitors.
Humans
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Poly(ADP-ribose) Polymerase Inhibitors/adverse effects*
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Phthalazines/pharmacology*
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Poly(ADP-ribose) Polymerases
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Fatigue/drug therapy*
2.Effects of PTK787 on cell proliferation and expression of fak mRNA in K562.
Xiao-Hua DI ; Ri-Ling CHEN ; Xiao-Li LIU ; Chuan TIAN ; Ya-Nan GUO
Journal of Experimental Hematology 2010;18(3):597-600
The aim of this study was to investigate the effects of tyrosine kinase inhibitor PTK787 on cell proliferation, cell cycle and the expression of fak mRNA of human chronic myeloid leukemia (CML) cell line K562, and to explore the mechanism of PTK787 against acute myeloid leukemia. The MTT method was used to detect the effects of PTK787 in various concentrations and at different time points on proliferation of K562 cells; the flow cytometry was used to determine the effects of PTK787 in different concentrations on cell cycle of K562 cells; the RT-PCR was used to assay the expression of fak mRNA in K562 cells treated with PTK787 for 48 hours. The results showed that along with increasing of the concentration and prolonging of time, the inhibitory rate of PTK787 on K562 proliferation was gradually enhanced. The comparison between various concentration groups at same time or comparison between various time groups in same concentration showed significant differences (p < 0.05), in which the effect of 320 micromol/L PTK787 on cells was strongest, while the continuous increase of PTK787 concentration or prolong of action time did not enhance the inhibitory rate on K562 proliferation. With increasing of drug concentration, the cell proportion in G(1) phase gradually increased, the cell proportion in S phase gradually decreased, the comparison between various groups revealed significant differences (p < 0.05), however the continuous increase of drug concentration from 160 micromol/L did not obviously change the cell proportion in phases of cell cycle. With increasing of drug concentration, the expression of fak mRNA in K562 cells gradually reduced with significant differences between various groups (p < 0.05), but with continuous increase of drug concentration from 160 micromol/L, the effect of PTK787 on the expression of fak mRNA in K562 cells also did not obviously change. It is concluded that the PTK787 shows effect of anti-leukemia cells through inhibiting transformation of the K562 cells from G(1) phase into S phase and decreasing the expression of fak mRNA in cells.
Cell Cycle
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Cell Proliferation
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drug effects
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Focal Adhesion Kinase 1
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genetics
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Gene Expression Regulation, Leukemic
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Humans
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K562 Cells
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Phthalazines
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pharmacology
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Pyridines
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pharmacology
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RNA, Messenger
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genetics
3.Effects of aldose reductase on the expression of fibronectin and collagen IV in cultured rat renal mesangial cells.
Tao JIANG ; Qi CHE ; Zhong-hua ZHAO ; Xiu-rong ZHANG ; Nong ZHANG
Chinese Journal of Pathology 2005;34(3):171-174
OBJECTIVETo study the effect of aldose reductase (AR) on expression of fibronectin and collagen IV in cultured rat renal mesangial cells (MsC).
METHODSAR expression plasmid vector (pCDNA3-AR) was constructed by restriction endonuclease digestion and ligation procedures. Stable expression of AR in MsC was established by Lipofectin transfection. Western blot and immunofluorescence analyses were performed to verify the transfection efficiency. Expression of fibronectin and collagen IV proteins were analyzed using Western blot.
RESULTSExpression of fibronectin and collagen IV in naive MsC treated with TGF-beta1 was upregulated in comparison to that of the untreated naive MsC (P < 0.01). MsC transfected with pCDNA3-AR showed a remarkable increase of expression of fibronectin and collagen IV (P < 0.01). Aldose reductase inhibitors (Sorbinil and Zopolrestat) significantly inhibited the expression of fibronectin and collagen IV in naive MsC (P < 0.05).
CONCLUSIONSOverexpression or inhibition of AR activity significantly alters the expression of fibronectin and collagen IV proteins in cultured rat MsC, suggesting that AR plays a significant role in the pathogenesis of glomerulosclersis.
Aldehyde Reductase ; antagonists & inhibitors ; genetics ; metabolism ; Animals ; Benzothiazoles ; pharmacology ; Cells, Cultured ; Collagen Type IV ; metabolism ; Fibronectins ; metabolism ; Genetic Vectors ; Imidazolidines ; pharmacology ; Mesangial Cells ; metabolism ; Phthalazines ; pharmacology ; Plasmids ; Rats ; Recombinant Proteins ; genetics ; metabolism ; Transfection ; Transforming Growth Factor beta1 ; pharmacology
4.Effect of glycine site/NMDA receptor antagonist MRZ2/576 on the conditioned place preference and locomotor activity induced by morphine in mice.
Yong-ping ZHU ; Zai-hao LONG ; Ming-lan ZHENG ; Ralf BINSACK
Journal of Zhejiang University. Science. B 2006;7(12):998-1005
OBJECTIVETo study the effect of glycine site/NMDA (N-methyl-D-aspartate) receptor antagonist MRZ2/576 on the conditioned place preference (CPP) and locomotor activity induced by morphine in mice.
METHODSDifferent doses (1.25, 2.5 and 5 mg/kg, i.p.) of MRZ2/576 were used to evaluate the effect of MRZ2/576 on the acquisition and expression of CPP induced by morphine (5 mg/kg) in mice. In addition, we examined the locomotor activity of mice in conditioning and testing phase of CPP paradigm.
RESULTSMRZ2/576 alone could not establish place preference, but a 5 mg/kg dose of MRZ2/576 could block both acquisition and expression of morphine-induced CPP. In testing phase of CPP, there was no statistical difference for locomotor activity between the groups; injection of MRZ2/576 showed a dose-dependent decrease of locomotor activity on both control and morphine-treated mice, especially 5 mg/kg of MRZ2/576 significantly suppressed the locomotor activity of mice.
CONCLUSIONBased on the present results, we assume that MRZ2/576 can antagonize the rewarding effect of morphine, suggesting that this glycine site/NMDA receptor antagonist could be used to treat addictions due to its light side effect profile.
Animals ; Conditioning (Psychology) ; drug effects ; Excitatory Amino Acid Antagonists ; pharmacology ; Magnesium ; physiology ; Male ; Mice ; Mice, Inbred ICR ; Morphine ; pharmacology ; Motor Activity ; drug effects ; Phthalazines ; pharmacology ; Receptors, N-Methyl-D-Aspartate ; antagonists & inhibitors
5.Synthesis and immunosuppressive effects of novel phthalazine ketone derivatives.
Ya-Li WANG ; Qing-He WANG ; Hong-Guang YANG ; Bo-Jun HAO ; Guo-Dong LIANG ; Chong-Guo JIANG ; Mao-Sheng CHENG
Acta Pharmaceutica Sinica 2013;48(10):1579-1584
A series of phthalazine ketone compounds were synthesized and the structures were confirmed by H NMR and HR-MS spectrum. All target compounds were obtained through 7 steps, including selective reduction, nitration, bromination, ring enlargement, reduction, Knoevenagel and acylated reaction. The compounds were evaluated for their immunosuppressive effects of T-cell proliferation and inhibitory activity of IMPDH type II in vitro, as well as their structure-activity relationship were assessed. Several compounds exhibited strong immunosuppressive properties, especially compounds 7f and 7h, with IC50 values of 0.093 micromol x L(-1) and 0.14 micromol x L(-1) respectively, which were superior to mycophenolic acid. The information obtained from the studies may be useful for further research on the immunosuppressive agents.
Animals
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Cell Proliferation
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drug effects
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Female
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IMP Dehydrogenase
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metabolism
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Immunosuppressive Agents
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chemical synthesis
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chemistry
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pharmacology
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Inhibitory Concentration 50
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Mice
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Mice, Inbred BALB C
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Phthalazines
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chemical synthesis
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chemistry
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pharmacology
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Spleen
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cytology
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Structure-Activity Relationship
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T-Lymphocytes
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drug effects
6.Effects of aldose reductase transfection on the proliferation of rat mesangial cells in vitro.
Qi CHE ; Tao JIANG ; Yi-feng LIN ; Hui LI ; Nong ZHANG
Chinese Journal of Pathology 2005;34(7):417-420
OBJECTIVETo study the effects of aldose reductase (AR) on the proliferation of rat mesangial cells (MsC) in vitro and to investigate its mechanism.
METHODSCell proliferation was assessed by MTT colorimetric assay. Cell cycle and apoptosis were analyzed by flow cytometry. The growth of normal MsC and AR transfected MsC was compared. The proliferation of PDGF-BB and cellular growth stimulation by 10% NBS were investigated using AR inhibitors (ARI) Sorbinil and Zopolrestat. The effects of PDGF-BB on the expression of AR, p65 and c-Jun were assessed by Western blot. Activation of AP-1 was measured by EMSA.
RESULTSAR expression of transfected MsC was distinctly higher than that of the control. Transfected MsC grew quicker than normal cells. ARI partially inhibited the proliferation of transfected MsC under the stimulation of PDGF-BB and 10% NBS, whereas 10% NBS had no effect on normal MsC. PDGF-BB upregulated the expression of AR and c-Jun, but had no effect on p65. The upregulation of c-Jun and the activation of AP-1 could be attenuated by ARI.
CONCLUSIONAR may participate in the pathological proliferation of MsC through the pathway related to the activation of AP-1.
Aldehyde Reductase ; antagonists & inhibitors ; genetics ; metabolism ; Animals ; Benzothiazoles ; pharmacology ; Cell Cycle ; Cell Proliferation ; Cells, Cultured ; Genetic Vectors ; Imidazolidines ; pharmacology ; Mesangial Cells ; cytology ; metabolism ; Phthalazines ; pharmacology ; Platelet-Derived Growth Factor ; pharmacology ; Proto-Oncogene Proteins c-jun ; metabolism ; Proto-Oncogene Proteins c-sis ; Rats ; Transcription Factor AP-1 ; metabolism ; Transfection
7.Effect of PKC signalling pathway and aldose reductase on expression of fibronectin induced by transforming growth factor-β1 in human mesangial cells.
Ping HUANG ; Yue-juan ZHANG ; Yuan HUANG ; Jing-jing ZHAO ; Tao JIANG ; Nong ZHANG
Chinese Journal of Pathology 2010;39(6):405-409
OBJECTIVETo study the effect of PKC signalling pathway and aldose reductase (AR) on the expression of fibronectin (FN) induced by transforming growth factor-β1 (TGF-β1).
METHODSHuman mesangial cells (HMCs) were cultured and transfected with pcDNA3-AR, and subject to AR gene silencing with small interfering RNA (siRNA) and then the cell was treated with recombinant human TGF-β1. The AR mRNA expression in the HMCs was examined using real time RT-PCR and protein expression of AR and FN was detected by Western blotting.
RESULTSThe cultured HMC treated with TGF-β1 showed increased expression of AR and FN, the normal HMC showed not reduced expression of FN after incubation with single inhibitors of AR.Pre-incubation of cells with inhibitors of AR and PKC, then the different groups of cells were treated with TGF-β1, and the induction effect on FN expression was suppressed (34%) in HMC. HMCs transfected with AR showed a strong protein expression of FN, which was increased by 3.6-fold after treatment with TGF-β1 (P < 0.05), and the induction effect on FN expression was suppressed by GÖ6983 (42%) in HMCs (P < 0.05). The HMC with AR gene knock-down by siRNA showed a decreased expression of AR and 90% decrease of FN protein in HMCs (P < 0.01), and TGF-β1-induced up-regulation of FN was significantly suppressed by siRNA (12%) in HMCs (P < 0.01).
CONCLUSIONSAR is capable of regulating FN expression only in the presence of TGF-β1, and this reaction is possibly accomplished through the activation of PKC signalling pathway.
Aldehyde Reductase ; antagonists & inhibitors ; biosynthesis ; genetics ; Benzothiazoles ; pharmacology ; Carbazoles ; pharmacology ; Cells, Cultured ; Fibronectins ; metabolism ; Gene Knockdown Techniques ; Humans ; Indoles ; Maleimides ; Mesangial Cells ; cytology ; metabolism ; Phthalazines ; pharmacology ; Protein Kinase C ; antagonists & inhibitors ; metabolism ; RNA, Messenger ; metabolism ; RNA, Small Interfering ; genetics ; Signal Transduction ; Transfection ; Transforming Growth Factor beta1 ; pharmacology ; Up-Regulation