Phosphoglucomutase was studied by polyacrylamide gel electrophoresis in the Thailand and Hawaii isolates of Parastrongylus cantonensis (also known as Angiostrongylus cantonensis). Two loci were present. The faster-moving locus (PGM-1) was polymorphic in the Hawaii isolate, represented by two alleles - the faster-moving, less common Pgm-1A and the slower-moving, more common Pgm-1B . It was monomorphic for the faster-moving allele Pgm-1A in the Thailand isolate. The slower-moving locus (PGM-2) was invariant, with a single band of enzyme activity, in the female worms of both the Thailand and Hawaii isolates. There was no detectable enzyme activity at this PGM-2 locus in the male worms of both isolates. The non-expression or 'null' PGM-2 phenotype in the male worms was presumed to be sex- limited. The present findings differ significantly in several aspects (polymorphic locus, proportion of polymorphic loci, heterozygosity, deviations from Hardy-Weinberg expectations, sex-limited expression) from the Japan isolate of P. cantonensis reported in the literature.
Gender ; 7-hydroxy-5,11-dioxotetranorprostane-1,16-dioic acid ; Thailand ; Phosphoglucomutase ; Hawaii

OBJECTIVE: PGM1 genotyping by PCR-SSCP analysis. METHODS: Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes. RESULTS: 2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains. CONCLUSION PGM1 system typed by PCR-SSCP is useful for forensic identification.
Alleles ; Asian People/genetics* ; China ; DNA/genetics* ; Gene Frequency ; Genotype ; Humans ; Phosphoglucomutase/genetics* ; Polymerase Chain Reaction/methods* ; Polymorphism, Genetic ; Polymorphism, Single-Stranded Conformational ; Sensitivity and Specificity

Aged ; Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Carcinoma, Signet Ring Cell ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Gastrectomy ; methods ; Histiocytic Sarcoma ; metabolism ; pathology ; surgery ; Humans ; Lymphatic Metastasis ; Male ; Phosphoglucomutase ; metabolism ; Stomach Neoplasms ; metabolism ; pathology ; surgery

OBJECTIVETo study the histogenesis of giant cell tumor (GCT) and factors related to tumor recurrence, invasiveness and malignant transformation.

METHODSThe clinical features, radiologic classification, surgical approach, pathologic findings, immunophenotypes and follow-up data of 123 cases of GCT were analyzed.

RESULTSThere was a significant correlation between tumor recurrence and radiographic classification (P = 0.032), over-expression of CD147 (P = 0.034) and p53 (P = 0.005), and surgical approach (P = 0.0048) in GCT. The biologic behavior showed no correlation with intramedullary infiltration, cortical bone involvement, parosteal soft tissue extension, tumor thrombi, fusiform changes of mononuclear tumor cells, mitotic count, Ki-67 index, coagulative tumor necrosis, secondary aneurysmal bone cyst formation, and adjoining bony reaction. The positive rate of p63 in stromal cells of GCT (79.7%, 94/118) was significantly higher than that in chondroblastoma (44.7%, 21/47), osteosarcoma (22.2%, 10/45) and other giant cell-rich tumors.

CONCLUSIONSGCT is a bone tumor of low malignant potential. It is sometimes characterized by locally invasive growth, active proliferation, coagulative necrosis, secondary aneurysmal bone cyst and surrounding bony reaction. It is difficult to predict the biologic behavior of GCT. Over-expression of p53 in the tumor cells and CD147 in all components of GCT correlate with tumor invasiveness, recurrence and malignant transformation. Selection of suitable surgical approach with reference to radiologic classification is considered as an important factor in reducing the recurrence rate.

Adolescent ; Adult ; Aged ; Basigin ; metabolism ; Bone Neoplasms ; diagnostic imaging ; drug therapy ; metabolism ; pathology ; surgery ; Chemotherapy, Adjuvant ; Female ; Follow-Up Studies ; Giant Cell Tumor of Bone ; diagnostic imaging ; drug therapy ; metabolism ; pathology ; surgery ; Humans ; Male ; Membrane Proteins ; metabolism ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Recurrence, Local ; Osteosarcoma ; pathology ; Phosphoglucomutase ; metabolism ; Radiography ; Tumor Suppressor Protein p53 ; metabolism ; Young Adult