OBJECTIVE: PGM1 genotyping by PCR-SSCP analysis. METHODS: Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes. RESULTS: 2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains. CONCLUSION PGM1 system typed by PCR-SSCP is useful for forensic identification.
Alleles ; Asian People/genetics* ; China ; DNA/genetics* ; Gene Frequency ; Genotype ; Humans ; Phosphoglucomutase/genetics* ; Polymerase Chain Reaction/methods* ; Polymorphism, Genetic ; Polymorphism, Single-Stranded Conformational ; Sensitivity and Specificity