1.Relationship between lysophosphatide acid acyltransferase beta and tumor - review.
Journal of Experimental Hematology 2008;16(4):975-978
Phosphatide acid (PA) is a kind of multifunctional bioactive phospholipid. It has been proved that PA produced by lysophosphatide acid acyltransferase (LPAATbeta) was involved in several signalling pathways in tumor cells, leading to the proliferation, apoptosis, migration, invasion, respiratory burst, expression and release of cytokine form tumor cells. The fact that expression of LPAATbeta was higher in tumor tissues than in their homologous normal tissues, and that antitumor effect of inhibitng LPAATbeta on solid tumor and hematological malignancy suggested that the targeting LPAATbeta would be a promising method of antitumor treatment. In this paper, the relevant basic and preclinical researches of LPAATbeta on antitumor treatment were summarized.
Acyltransferases
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antagonists & inhibitors
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genetics
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metabolism
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Humans
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Neoplasms
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drug therapy
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enzymology
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Phosphatidic Acids
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metabolism
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physiology
2.Phospholipase D activates HIF-1-VEGF pathway via phosphatidic acid.
Songyi HAN ; Jeongsoon HUH ; Wooseong KIM ; Seongkeun JEONG ; Do Sik MIN ; Yunjin JUNG
Experimental & Molecular Medicine 2014;46(12):e126-
Growth factor-stimulated phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine (PC), generating phosphatidic acid (PA) which may act as a second messenger during cell proliferation and survival. Therefore, PLD is believed to play an important role in tumorigenesis. In this study, a potential mechanism for PLD-mediated tumorigenesis was explored. Ectopic expression of PLD1 or PLD2 in human glioma U87 cells increased the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) protein. PLD-induced HIF-1 activation led to the secretion of vascular endothelial growth factor (VEGF), a HIF-1 target gene involved in tumorigenesis. PLD induction of HIF-1alpha was significantly attenuated by 1-butanol which blocks PA production by PLD, and PA per se was able to elevate HIF-1alpha protein level. Inhibition of mTOR, a PA-responsive kinase, reduced the levels of HIF-1alpha and VEGF in PLD-overexpressed cells. Epidermal growth factor activated PLD and increased the levels of HIF-1alpha and VEGF in U87 cells. A specific PLD inhibitor abolished expression of HIF-1alpha and secretion of VEGF. PLD may utilize HIF-1-VEGF pathway for PLD-mediated tumor cell proliferation and survival.
Cell Line, Tumor
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Epidermal Growth Factor/metabolism
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Gene Expression Regulation, Neoplastic
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Glioma/genetics/*metabolism
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit/genetics/metabolism
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Phosphatidic Acids/*metabolism
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Phospholipase D/genetics/*metabolism
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*Signal Transduction
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Transfection
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Vascular Endothelial Growth Factor A/*metabolism
3.STAT3 is involved in phosphatidic acid-induced Bcl-2 expression in HeLa cells.
Hye Jin CHOI ; Jung Han LEE ; Shin Young PARK ; Ju Hwan CHO ; Joong Soo HAN
Experimental & Molecular Medicine 2009;41(2):94-101
Phosphatidic acid (PA), the product of a PLD-mediated reaction, is a lipid second messenger that participates in various intracellular signaling events and is known to regulate a growing list of signaling proteins. We found that Bcl-2 was upregulated by PA treatment in HeLa cells. However, how PA upregulates Bcl-2 expression has not yet been studied. In this study, we tried to discover the mechanisms of Bcl-2 up-regulation by PA treatment in HeLa cells. Treatment with PA resulted in significantly increased expression of Bcl-2 in HeLa cells. Moreover, PA-induced Bcl-2 expression was blocked by mepacrine, an inhibitor of PLA2, but not by propranolol, an inhibitor of PA phospholyhydrolase (PAP). Treatment of 1,2-dipalmitoryl-sn-glycero-3-phosphate (DPPA) also increased Bcl-2 expression. These results indicate that Bcl-2 expression is mediated by lysophosphatidic acid (LPA), not by arachidonic acid (AA). Thereafter, we used MEK1/2 inhibitor, PD98059 to investigate the relationship between ERK1/2 MAPK and PA-induced Bcl-2 expression. PA-induced Bcl-2 expression was decreased when ERK1/2 was inhibited by PD98059. The transcription factor such as STAT3 which is controlled by ERK1/2 MAPK was increased along with Bcl-2 expression when the cells were treated with PA. Furthermore, STAT3 siRNA treatments inhibited PA-induced Bcl-2 expression, suggesting that STAT3 (Ser727) is involved in PA-induced Bcl-2 expression. Taken together, these findings indicate that PA acts as an important mediator for increasing Bcl-2 expression through STAT3 (Ser727) activation via the ERK1/2 MAPK pathway.
Enzyme Inhibitors/pharmacology
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Gene Expression Regulation, Neoplastic
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Hela Cells
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Humans
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Mitogen-Activated Protein Kinase Kinases/genetics/metabolism
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Phosphatidic Acids/*genetics/metabolism
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Propranolol/pharmacology
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Proto-Oncogene Proteins c-bcl-2/*genetics/metabolism
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Quinacrine/pharmacology
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RNA, Small Interfering/genetics
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STAT3 Transcription Factor/*genetics/metabolism