1.Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms.
Danielle KIAN ; Cesar Armando Contreras LANCHEROS ; Igor Alexandre Campos DAMIANI ; Tamiris Zanforlin Olmos FERNANDES ; Phileno PINGE-FILHO ; Marcia Regina Machado DOS SANTOS ; Jose Franco DA SILVEIRA ; Celso Vataru NAKAMURA ; Joao Santana DA SILVA ; Sueli Fumie YAMADA-OGATTA ; Lucy Megumi YAMAUCHI
The Korean Journal of Parasitology 2015;53(4):483-488
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
Amino Acid Sequence
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Chagas Disease/*parasitology
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Gene Expression Regulation, Developmental
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Humans
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Life Cycle Stages
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Molecular Sequence Data
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Protozoan Proteins/*genetics/metabolism
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Sequence Alignment
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Trypanosoma cruzi/*genetics/*growth & development/isolation & purification/metabolism